scholarly journals Serendipitous In Situ Conservation of Faba Bean Landraces in Tunisia: A Case Study

Genes ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 236 ◽  
Author(s):  
Elyes Babay ◽  
Khalil Khamassi ◽  
Wilma Sabetta ◽  
Monica Marilena Miazzi ◽  
Cinzia Montemurro ◽  
...  
Keyword(s):  
Faba Bean ◽  
Agricultural Research ◽  
In Situ Conservation ◽  
Grain Legumes ◽  
Gene Bank ◽  
Genetic Constitution ◽  
Special Focus ◽  
North West ◽  
The North

Cultivation of faba bean (Vicia faba L.) in Tunisia is largely based on improved varieties of the crop. However, a few farmers continue to produce local cultivars or landraces. The National Gene Bank of Tunisia (NGBT) recently launched a collection project for faba bean landraces, with special focus on the regions of the North West, traditionally devoted to cultivating grain legumes, and where around 80% of the total national faba bean cultivation area is located. The seed phenotypic features of the collected samples were studied, and the genetic diversity and population structure analyzed using simple sequence repeat markers. The genetic constitution of the present samples was compared to that of faba bean samples collected by teams of the International Center for Agricultural Research in the Dry Areas (ICARDA) in the 1970s in the same region, and stored at the ICARDA gene bank. The results of the diversity analysis demonstrate that the recently collected samples and those stored at ICARDA largely overlap, thus demonstrating that over the past 50 years, little genetic change has occurred to the local faba bean populations examined. These findings suggest that farmers serendipitously applied international best practices for in situ conservation of agricultural crops.

Comparative evaluation of red clover genotypes with the “Pskovskiy mestnyy dvuukosnyy” variety

2021 ◽  
pp. 16-21
Author(s):  
А.М. Мазин ◽  
Э.Э. Егги
Keyword(s):  
Agricultural Research ◽  
Negative Impact ◽  
Plant Genetic Resources ◽  
Red Clover ◽  
Parent Material ◽  
Agricultural Research Institute ◽  
North West ◽  
High Productivity ◽  
The North ◽  
Research Institute

В статье рассмотрены приёмы восстановления высокоурожайного сорта клевера лугового Псковский местный двуукосный селекции Псковского НИИСХ с целью возвращения его в сельскохозяйственное производство Северо-Западного региона РФ. Сорт Псковский местный двуукосный отличается высокой урожайностью, продуктивным долголетием и устойчивостью к неблагоприятным факторам среды. Имеет повышенный спрос у производственников Псковской области. Однако отсутствие семеноводства, проблемы с выдачей сортовых и посевных документов сдерживают его распространение. Большой интерес представляет использование данного сорта в селекционной работе. В 2018–2020 годах в питомнике ОП Псковского НИИСХ были изучены семь образцов этой культуры (П-101–П-107), полученных из хозяйств области. Посеянные семена собраны с неапробированных посевов предположительно сорта Псковский местный двуукосный, который возделывался в хозяйствах многие годы. Для сравнительного анализа оригинал сорта получили из коллекции ВИР (К-31744). В результате определения типа созревания образцов по количеству междоузлий на стебле и построения вариационных кривых образцы П-103, П-106, П-107 отнесли к позднеспелому типу клевера лугового, что указало на их несоответствие оригиналу. Наиболее близкими по этому показателю к оригиналу оказались образцы П-101, П-102, П-104, П-105. По морфологическим признакам наибольшее сходство с оригиналом имели образцы П-101, П-102, П-103. Методом SDS-электрофореза белковых спектров выявили три позиции, по которым исследованные образцы неапробированных посевов (П-101, П-102, П-103) отличались от К-31744. Для восстановления сорта Псковский местный двуукосный в качестве исходного материала перспективно использование образцов П-101 и П-102 как наиболее схожих с оригиналом по срокам созревания и морфологии. Метод SDS-электрофореза позволяет контролировать селекционные образцы на идентичность сортам клевера лугового. This article reports on the experiment aimed at improving the performance of red clover “Pskovskiy mestnyy dvuukosnyy” to be cultivated in the North-West region of Russia. The variety was bred at the Pskov Agricultural Research Institute and has high productivity, productive longevity and resistance to environmental stresses. This variety is well-known among the farmers in the Pskov region. However poor seed production together with paper issues have negative impact on its wide cultivation. “Pskovskiy mestnyy dvuukosnyy” is a valuable material for plant breeding. In 2018–2020 the Pskov Agricultural Research Institute collected seven red clover specimens (P-101–P-107) from the regional farms. The collected seeds potentially belonged to the “Pskovskiy mestnyy dvuukosnyy” variety. The original variety was obtained from the Federal Research Center N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) as a control (K-31744). As a result, P-103, P-106, and P-107 were shown to be long-seasoned and therefore differed from the control. P-101, P-102, P-104, and P-105 were more similar to the control in their maturation rate. However, P-101, P-102, P-103 morphologically were closer to the original variety. SDS-PAGE showed that P-101, P-102, and P-103 differed from the control (K-31744). P-101 and P-102 were used as parent material to improve “Pskovskiy mestnyy dvuukosnyy” since they were more similar to the one.

scholarly journals Advances in fruit breeding in Nepal

2020 ◽  
Vol 3 (1) ◽  
pp. 301-319
Author(s):  
Tek P Gotame ◽  
Ishwori P Gautam ◽  
Surendra L Shrestha ◽  
Jiban Shrestha ◽  
Bal Krishna Joshi
Keyword(s):  
Fruit Development ◽  
Agricultural Research ◽  
National Level ◽  
Germplasm Collection ◽  
Gene Bank ◽  
Special Focus ◽  
Research Station ◽  
Fruit Breeding ◽  
Tropical Fruit ◽  
Protocol Standardization

Nepal has spent about six decades on fruit development and research in different species. Fruit breeding particularly local and exotic germplasm collection started after 1950s and has gained momentum after the formation of commodity programme in 1972 AD. Major researches in the past were focused on indigenous and exotic genotype collection, evaluation, selection, propagation protocol standardization and adoptive trials. Some good ground works have already been done in major fruits such as apple, pear, plum, persimmon, kiwifruit, citrus, litchi, guava, pomegranate, walnut, papaya, banana and mango. The major public institutions involved in fruit breeding are Nepal Agricultural Research Council (NARC) following Agriculture and Forestry University. Some of the private nurseries like Everything Organic Nursery, Kavre and Technology Demonstration Centre of ICIMOD, Lalitpur were also involved in introduction and maintenance of indigenous and exotic fruit species. National Centre for Fruit Development, Kirtipur; Tropical Region Horticulture Centre, Nawalpur; Temperate Horticulture Farm, Satbanj, and Horticulture Farm, Marpha collected many local and exotic fruits and maintained at field gene bank. Horticulture Research Station, Rajikot has introduced 25 spur type apple cultivars and maintained in field gene bank. National Citrus Research Programme, Paripatle has introduced, collected and maintained 130 genotypes including exotic and indigenous landraces of citrus. Two varieties of acid lime ‘Sunkagati-1’ and ‘Sunkagati-2’ have been released and one variety ‘Terhathum Local’ has been registered. ‘Khoku Selection’ of mandarin orange has also been registered. Banana varieties 'Malbhog', 'Willium Hybrid' and 'G9' has been selected by participatory varietal selection and registered.  To strengthen fruit breeding in the nation, NARC needs to be restructured with special focus on fruit researches. Establishment of national fruit commodity programs along with establishment of Tropical Fruit Research Station in Province 2 and Temperate Fruit Research Station in Province 5 at national level can streamline NARC's fruit breeding researches.

Evolution of Grain Legumes. I. Mediterranean Pulses

1984 ◽  
Vol 20 (4) ◽  
pp. 275-296 ◽  
Author(s):  
J. Smartt
Keyword(s):  
Faba Bean ◽  
Geographic Origin ◽  
Grain Legumes ◽  
Grass Pea ◽  
The North ◽  
Fertile Crescent ◽  
The Common ◽  
Points Of View ◽  
The Mediterranean ◽  
Cultivated Populations

SUMMARYThe evolution of the Mediterranean pulses, the common pea (Pisum sativum L.), the faba bean (Vicia faba L.), the grass pea (Lathyrus sativus L.), the lentil (Lens culinaris Medik.) and the chickpea (Cicer arietinum L.), is considered from the points of view of geographic origin and subsequent dissemination. All appear to have originated in the Fertile Crescent, the most significant subsequent spread being to the north of the Mediterranean basin in the case of the common pea and the faba bean and to the east of it in the case of the other three species. The wild progenitor type is known for all species except the faba bean. In the grass pea the extent of divergence between wild and cultivated populations is small, possibly due to its use predominantly for forage and less as a pulse. Considerable divergence has occurred in other species, where use as a pulse is more important.

scholarly journals On the Late Pliocene stone tools of the Quranwala Zone, north-west sub-Himalayas, India

Antiquity ◽  
2017 ◽  
Vol 91 (357) ◽  
Author(s):  
Vidwan Singh Soni ◽  
Anujot Singh Soni
Keyword(s):  
Stone Tools ◽  
Fossil Bone ◽  
Question Mark ◽  
North West ◽  
Cut Marks ◽  
The North ◽  
Geological Processes ◽  
Animal Bones ◽  
Fossil Bones

A recent study of the Quranwala Zone (QZ) of the north-west sub-Himalayas, India, presents evidence for anthropic activity during the Pliocene that includes a number of stone tools found in association with fossil animal bones with cut marks. Based on the date of the Pliocene rock outcrop, the tools and bones are suggested to date from 2.6 Ma (Gaillard et al. 2016). There is, however, a question mark over the context of these tools within an outcrop of Pliocene rocks and, hence, over the date of these tools and the fossil bones. The trench from which they were excavated at Masol 2 (Gaillard et al.2016: fig. 3) lies in a depression at the bottom of a slope; the description provided in section 2 of the paper by Gaillard et al. (2016) suggests that the stone tools may not have been in situ within the Pliocene levels, but had accumulated there and were mixed with the fragments of fossil bone due to geological processes. Moreover, many of the stone tools, such as the ‘simple choppers’ found in association with the fossil animal bones (Gaillard et al.2016: figs 6, 8, 9), are usually found on much more recent sites and are therefore unlikely to date from 2.6 Ma.

scholarly journals Analysis of Genetic Diversity and Population Structure of Orobanche foetida Populations From Tunisia Using RADseq

2021 ◽  
Vol 12 ◽  
Author(s):  
Amal Boukteb ◽  
Shota Sakaguchi ◽  
Yasunori Ichihashi ◽  
Mohamed Kharrat ◽  
Atsushi J. Nagano ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Faba Bean ◽  
Isolation By Distance ◽  
Distance Effect ◽  
Wild Plant ◽  
Clustering Methods ◽  
North West ◽  
North East ◽  
The North

Orobanche foetida Poiret is a holoparasitic plant that lacks chlorophyll and totally depending on its host for its growth. Orobanche foetida parasitizes host plant roots and extract nutrient and water via a haustorium. Although O. foetida distributes in the Mediterranean region as a wild plant parasite, it parasitizes faba bean causing serious damages which may reach 90% yield losses in Tunisia. Analysis of genetic diversity of the parasite is important to better understand its evolution and spread, remained largely unknown. In this work, we present the first study on genetic diversity and population structure using the robust technique Restriction-site-Associated DNA sequencing (RADseq) for Orobanche spp. We collected 244 samples of O. foetida from 18 faba bean fields in the north of Tunisia including 17 populations from the north-west and one population form the north-east. To overcome the difficulty of SNP discovery in O. foetida genome as a non-model and tetraploid plant, we utilized three different informatics pipelines, namely UNEAK, pyRAD and Stacks. This study showed that genetic differentiation occurred in the Tunisian O. foetida emphasizing the isolation by distance effect. However, no strong population clustering was detected in this work basing on the three data sets and clustering methods used. The present study shed the light on the current distribution and the genetic variation situation of the fetid broomrape in Tunisia, highlighting the importance of understanding the evolution of this parasite and its genetic background. This will aid in developing efficient strategies to control this parasite and its expansion in Tunisia and worldwide.

scholarly journals International Coconut Genetic Resources Network (COGENT): Its history and achievements

CORD ◽  
2005 ◽  
Vol 21 (02) ◽  
pp. 34
Author(s):  
Pons Batugal
Keyword(s):  
Latin America ◽  
Genetic Resources ◽  
International Development ◽  
Agricultural Research ◽  
Plant Genetic Resources ◽  
In Situ Conservation ◽  
Research Network ◽  
Ex Situ

The International Coconut Genetic Resources Network (COGENT) is a global research network organized by the International Plant Genetic Resources Institute (IPGRI) in 1992 with support from member countries, the Consultative Group on International Agricultural Research (CGIAR), partner institutions, donor agencies, and by regional and international development organizations.                           In the last 12 years, COGENT has been fully operational with 38 member coconut producing countries in five regions (South Asia; Southeast and East Asia; South Pacific; Africa and the Indian Ocean; and Latin America and the Caribbean). It has successfully developed and disseminated to coconut breeders and curators worldwide the International Coconut Genetic Resources Database (CGRD). The CGRD contains characterization data and some pictures of 1,416 accessions which are conserved by national programmes in 28 sites in 23 countries. To further secure conserved germplasm, a COGENT multi-site International Coconut Genebank has been established to conserve 200 important accessions in each region. Coconut varieties with multi-purpose uses are being identified, documented and promoted. The performance of promising 38 high-yielding hybrids are being evaluated in a multilocation  trial involving four African and three Latin America/Caribbean countries to identify suitable varieties and hybrids for resource-poor farmers. Farmers’ varietal preferences in 15 countries are being evaluated. Diversity-linked income-generating activities are being used as a strategy to promote in situ and on-farm conservation and germplasm utilization have been initiated in 15 countries. Protocols for in vitro embryo culture, cryopreservation, morphometric and molecular marker-based methods for locating and characterizing diversity; pest risk assessment and germplasm health management are being developed, tested and upgraded. Strategies and techniques for farmer participatory research, collecting, characterization and ex situ and in situ conservation are being refined.   To strengthen the coconut research capability of COGENT member countries, the COGENT Secretariat and IPGRI have organized 39 country need assessment missions  and conducted 41 workshops and meetings involving 994 coconut researchers to share information and technologies, discuss issues and common problems and opportunities and how to address them; conducted 40 training courses involving 765 participants from 41 countries; supported 274  research and training/capacity building activities  in 30 countries; and led the establishment of the Global Coconut Research for Development Programme (PROCORD). IPGRI and COGENT's current priority involves the further promotion of more effective conservation and use of coconut genetic resources, both regionally and globally.

scholarly journals Potato mosaic viruses which infect plants of tuber-bearing Solanum spp. growing in the VIR field gene bank

2019 ◽  
Vol 23 (3) ◽  
pp. 304-311 ◽  
Author(s):  
E. V. Rogozina ◽  
N. V. Mironenko ◽  
N. A. Chalaya ◽  
Yu. Matsushita ◽  
H. Yanagisawa
Keyword(s):  
Potato Virus ◽  
North American ◽  
Solanum Species ◽  
Gene Bank ◽  
North American Species ◽  
Rt Pcr ◽  
Virus Diseases ◽  
North West ◽  
The North ◽  
Solanum Spp

Potato crop is particularly affected by virus diseases, and potato virus Y (PVY) currently considered the most important pathogen distributed worldwide as a diversity of strains. Wild and cultivated tuber-bearing species of the genus Solanum L., stored in the VIR collection, are used as the initial material in creation domestic potato varieties (Solanum tuberosum L.) resistant to virus diseases. The preservation and rational utilization of the potato collection is based on regular phytosanitary monitoring, including quarantine objects, foremost PSTVd (potato spindle tuber viroid). The aim of the work is to examine plants of tuber-bearing Solanum species in the field gene bank of VIR for the presence of PSTVd and PVX (potato virus X), PVS (potato virus S), PVM (potato virus M) and PVY, which are the most common viruses on potatoes in the North-West District of Russia. We examined clonal plants of 137 genotypes representing 31 species of the section Petota of the genus Solanum L. A diagnostic was carried out using ELISA, RT-PCR and indicator plants. No PSTVd was found in the studied plants, but a plural infestation by mosaic viruses was detected, more than half of the tested clones are infected with two or more viruses. In the studied samples, only 17 genotypes (12 %) are not infected by PVX, PVS, PVM and PVY according to the ELISA test. There are statistically significant differences in the virus infestation of Solanum species with different origins, according to Pearson’s chi-squared test. Among the studied genotypes of wild relatives of potatoes, the proportion of those affected by PVY was significantly higher in the South American than in the North American species (χ2 = 4.56, p = 0.03); the proportion of genotypes affected by PVХ was significantly higher in the North American species (χ2 = 8.81, p = 0.003), the critical value was χ2 = 3.841. PVY strains were identified by multiplex RT-PCR in 37 genotypes of Solanum spp. We found that 27 genotypes are infected by a common PVYO strain, two genotypes are infected by PVYNW (A) and PVYNW (B) strains, respectively, seven genotypes are infected by a mixture of PVYO +PVYNW (A) strains, and one is infected by a mixture of PVYO +PVYNTN-NW (SYRI)+SYRIII strains. The recombinant strains of PVY are detected in the North-West District of Russia for the first time. Coherency of the results of PVY strains detection by various (immunological, molecular and biological) methods is discussed.

scholarly journals Model-based aviation advice on distal volcanic ash clouds by assimilating aircraft in-situ measurements

2016 ◽  
Author(s):  
G. Fu ◽  
A. W. Heemink ◽  
S. Lu ◽  
A. J. Segers ◽  
K. Weber ◽  
...  
Keyword(s):  
Volcanic Ash ◽  
Transport Model ◽  
Real Life ◽  
Forecast Accuracy ◽  
In Situ Measurements ◽  
North West ◽  
The North ◽  
Ash Plume ◽  
Potential Improvement

Abstract. The forecast accuracy of distal volcanic ash clouds is important for providing valid aviation advice during volcanic ash eruption. However, because the distal part of volcanic ash plume is far from the volcano, the influence of eruption information on this part becomes rather indirect and uncertain, resulting in inaccurate volcanic ash forecasts in these distal areas. In our approach, we use real-life aircraft in-situ observations, measured in the North-West part of Germany during the 2010 Eyjafjallajokull eruption, in an ensemble-based data assimilation system combined with a volcanic ash transport model to investigate the potential improvement on the forecast accuracy with regard to the distal volcanic ash plume. We show that the error of the analyzed volcanic ash state can be significantly reduced through assimilating real-life in-situ measurements. After a continuous assimilation, it is shown that the aviation advice for Germany, the Netherlands and Belgium can be significantly improved. We suggest that with suitable aircrafts measuring once per day across the distal volcanic ash plume, the description and prediction of volcanic ash clouds in these areas can be greatly improved.

WELL-BORE INSTABILITY IN THE NORTH WEST SHELF OF AUSTRALIA

1994 ◽  
Vol 34 (1) ◽  
pp. 1
Author(s):  
C.P. Tan ◽  
D.R. Willoughby ◽  
R.R. Hillis ◽  
S. Zhou ◽  
H-B Mühlhaus ◽  
...  
Keyword(s):  
Research Centre ◽  
Well Bore ◽  
Cooperative Research ◽  
Stress Regime ◽  
North West ◽  
The North ◽  
In Situ Stresses ◽  
Stress Changes ◽  
Contour Plots

Well-bore instability, experienced mainly in shales, has resulted in significant drilling delays and abandonment of wells in the North West Shelf of Australia. Although these problems may be induced by physico-chemical interactions, there is an increasing awareness that instability in this region is principally associated with in situ stresses that are high relative to the strength of the materials.This paper describes the research undertaken by the Australian Petroleum Cooperative Research Centre to assist industry in understanding and managing stress-induced well-bore instability in this region. To conduct such stability analyses the basic information required includes knowledge of the orientation and magnitude of the principal in situ stresses and the strength and deformation response of the materials to stress changes imposed by drilling. The required data can be determined using welllogging, analytical and laboratory techniques.Analytical methods can be used to examine the relationship between well-bore stability and changes introduced through drilling. Spreadsheets based on the analytical methods have been produced and applied to the assessment of drilling alternatives and/or design of some well-bores in the North West Shelf.The application of the critical mud weight contour plots and mud weight stability profiles produced by the spreadsheets in assessing drilling alternatives, selection of optimum well-bore alignment and mud weight design are demonstrated through examples. The analyses showed that counter to intuitive expectations, an inclined well may be more stable than a vertical well depending on the well-bore direction, deviation angle and stress regime.

IV.—Note on the Fossils from the Glacial Deposits of the North-West Coast of the Island of Lewis, Outer Hebrides

1876 ◽  
Vol 3 (12) ◽  
pp. 552-555 ◽  
Author(s):  
R. Etheridge
Keyword(s):  
West Coast ◽  
Long Island ◽  
The Other ◽  
Glacial Deposits ◽  
Geological Society ◽  
North West ◽  
The North ◽  
Outer Hebrides

In a paper, read before the Geological Society of London (June 25, 1873), “ On the Glacial Phenomena of the Long Island or Outer Hebrides,” Mr. James Geikie mentioned the occurrence of two descriptions of stony clay in the Long Island. The first of these deposits or “Bottom Till” occupies the low ground of the island, and is derived from the disintegration chiefly of gneissie rocks, and so far as observation went proved to be unfossiliferous. The other deposit was found in the north of Lewis, along the coast, and consists of two beds of unstratified stony clay containing fragments of shells, the lower resting on rock in situ.

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