scholarly journals Mitochondrial DNA Haplotypes Influence Energy Metabolism across Chicken Transmitochondrial Cybrids

Genes ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 100
Author(s):  
Minghua Kong ◽  
Hai Xiang ◽  
Jikun Wang ◽  
Jian Liu ◽  
Xiben Zhang ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Oxygen Consumption ◽  
Primary Cells ◽  
Functional Differences ◽  
Native Chicken ◽  
Primary Cell Lines ◽  
Mitochondrial Dna Haplotype ◽  
Chicken Breeds ◽  
Tibetan Chicken ◽  
Shed Light

The association between mitochondrial DNA haplotype and productive performances has been widely reported in chicken breeds. However, there has not been physiological evidence of this seen previously. In this study, chicken transmitochondrial cells were generated using the nucleus of the DF-1 cell line and mitochondria of primary cell lines derived from two native chicken breeds, Tibetan chicken and Shouguang chicken. Generally, Tibetan chicken primary cells showed a stronger metabolic capacity than Shouguang chicken primary cells. However, the Tibetan chicken cybrids had a dramatic drop in relative mtDNA copies and oxygen consumption. Higher rates of oxygen consumption (OCR) and expression levels of mitochondrial biogenesis and fusion genes were observed in Shouguang chicken cybrids, potentially reflecting that the mitochondrial DNA haplotype of Shouguang chicken had better coordination with the DF-1 nucleus than others. Meanwhile, mitonuclear incompatibility occurred in Tibetan chicken cybrids. The results demonstrate functional differences among mitochondrial DNA haplotypes and may shed light on the interaction between the mitochondria and nucleus in Gallus gallus domesticus.

Genetic diversity of Jiangsu native chicken breeds assessed with the mitochondrial DNA D-loop region

2017 ◽  
Vol 59 (1) ◽  
pp. 34-39 ◽  
Author(s):  
X. X. Jia ◽  
J. X. Lu ◽  
X. J. Tang ◽  
Y. F. Fan ◽  
S. H. Huang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Loop Region ◽  
Native Chicken ◽  
D Loop ◽  
Chicken Breeds

​​Differential Disease Resistance of Indian Native Chicken Breeds to Experimental P. multocida Infection

2021 ◽  
Author(s):  
T.R. Kannaki ◽  
E. Priyanka ◽  
M. Abhilash ◽  
Santosh Haunshi
Keyword(s):  
Experimental Infection ◽  
Pasteurella Multocida ◽  
Clinical Signs ◽  
Mortality Rates ◽  
Susceptibility Pattern ◽  
Intranasal Route ◽  
Bacterial Diseases ◽  
Death Time ◽  
Native Chicken ◽  
Chicken Breeds

Background: Native chicken breeds are considered more disease tolerant than exotic chicken breeds especially for the bacterial diseases. Aseel, Ghagus and Vanaraja chicken breeds/ variety were evaluated for the disease tolerance/susceptibility pattern after experimental infection with P. multocida A:1 isolate. Methods: A total of 72 birds of three breeds viz., Aseel, Ghagus and Vanaraja (n=24 each) were divided into three groups. The birds were inoculated with 2.5x106 CFU/ml of virulent Pasteurella multocida A:1 isolate through intraperitoneal (I/P) and intranasal (I/N) routes at 12 weeks of age. Clinical signs, morbidity, mortality rates and lesions were observed in the infected birds. Result: The mortality rates were 83.3% in Assel breed against 100% in both Ghagus and Vanaraja breed in intraperitoneally infected groups. Upon intranasal infection, the mortality was 83.3% in Assel and Vanaraja breed against 100% in Ghagus breed. Aseel birds showed significantly better survivability and longer death time than Ghagus breed upon experimental infection with Pasteureall multocida A:1 isolate. Vanaraja breed showed tolerance comparable to Aseel in experimental infection via intranasal route.

Mitochondrial DNA Haplotype Diversity in Two Portunid Populations Found along the Georgia and South Carolina Coasts

2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Amy Abdulovic-Cui ◽  
Sierra Mannix ◽  
Austin Coleman ◽  
Brittany White ◽  
Keturah Mingledolph ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
South Carolina ◽  
Haplotype Diversity ◽  
Mitochondrial Dna Haplotype

scholarly journals Mitochondrial fission is required for proper nucleoid distribution within mitochondrial networks

2021 ◽  
Author(s):  
Hema Saranya Ilamathi ◽  
Mathieu Ouellet ◽  
Rasha Sabouny ◽  
Justine Desrochers-Goyette ◽  
Matthew A Lines ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Network Structure ◽  
Mitochondrial Fission ◽  
Healthy Population ◽  
Primary Cells ◽  
Mitochondrial Homeostasis ◽  
Mtdna Replication ◽  
Mitochondrial Networks ◽  
Regular Spacing

Mitochondrial DNA (mtDNA) maintenance is essential to sustain a functionally healthy population of mitochondria within cells. Proper mtDNA replication and distribution within mitochondrial networks are essential to maintain mitochondrial homeostasis. However, the fundamental basis of mtDNA segregation and distribution within mitochondrial networks is still unclear. To address these questions, we developed an algorithm, Mitomate tracker to unravel the global distribution of nucleoids within mitochondria. Using this tool, we decipher the semi-regular spacing of nucleoids across mitochondrial networks. Furthermore, we show that mitochondrial fission actively regulates mtDNA distribution by controlling the distribution of nucleoids within mitochondrial networks. Specifically, we found that primary cells bearing disease-associated mutations in the fission proteins DRP1 and MYH14 show altered nucleoid distribution, and acute enrichment of enlarged nucleoids near the nucleus. Further analysis suggests that the altered nucleoid distribution observed in the fission mutants is the result of both changes in network structure and nucleoid density. Thus, our study provides novel insights into the role of mitochondria fission in nucleoid distribution and the understanding of diseases caused by fission defects.

scholarly journals First Record of a Novel Mitochondrial DNA Haplotype of a Marble Trout Lineage of Salmo Trutta in Turkey

10.4194/ga452 ◽  
2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Fevzi Bardakci ◽  
Nazan Acar ◽  
Tulin Arslan ◽  
Riadh Badraoui
Keyword(s):  
Mitochondrial Dna ◽  
Salmo Trutta ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Aegean Sea ◽  
First Record ◽  
Unique Haplotype ◽  
Marble Trout ◽  
Mitochondrial Dna Control Region ◽  
Mitochondrial Dna Haplotype

A new record of a marble trout mtDNA haplotype known to be restricted to Adriatic basin (called marmoratus lineage within Salmo trutta complex) has been reported from Eşen Stream in the Aegean Sea basin of southeastern Turkey, based on sequence data of the mitochondrial DNA control region. The results of this study showed a single unique haplotype from this population, called MATR1. Phylogenetic analyses of this haplotype along with other haplotypes belonging to different mitochondrial DNA lineages of the S. trutta complex confirmed the existence of the marmoratus lineage in Turkey, suggesting a possible river capture between the Adriatic and Aegean Sea basins until the last (Würmian) marine regression.

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