scholarly journals Genome-Wide Identification of WRKY Transcription Factors in Chinese jujube (Ziziphus jujuba Mill.) and Their Involvement in Fruit Developing, Ripening, and Abiotic Stress

Genes ◽  
2019 ◽  
Vol 10 (5) ◽  
pp. 360 ◽  
Author(s):  
Xin Chen ◽  
Ruihong Chen ◽  
Yanfeng Wang ◽  
Cuiyun Wu ◽  
Jian Huang
Keyword(s):  
Transcription Factors ◽  
Fruit Ripening ◽  
Transcript Abundance ◽  
Northern China ◽  
Tissue Expression ◽  
Chinese Jujube ◽  
Wrky Transcription Factors ◽  
Genome Wide ◽  
Jujube Fruit ◽  
Ziziphus Jujuba

Chinese jujube (Ziziphus jujuba Mill.) is an economically important fruit crop in China and mainly cultivated on land with high salinity and drought conditions in northern China. WRKY transcription factors (TFs) are involved in plant development and in responses to multiple abiotic stresses. In this study, we identified 61 and 52 putative ZjWRKY TFs in ‘Junzao’ and ‘Dongzao’ at the genome-wide level. Tissue expression profiling showed that 7 genes were constitutively expressed at high level in all tissues of ‘Junzao’. Transcriptome analysis revealed that 39 ZjWRKY genes were expressed during ‘Junzao’ jujube fruit ripening. Among these genes, the transcript abundance of 19 genes were differentially expressed between ‘Junzao’ and ‘Qingjiansuanzao’ fruit. In addition, RT-qPCR analyses revealed that 30, 14, and 18 ZjWRKY genes responded to drought, NaCl, and ABA treatments, respectively. Taken together, ZjWRKY genes expression dynamics during jujube fruit development, ripening, and their differences between jujube and wild jujube would provide insights into their possible roles regulating fruit ripening. In addition, those ZjWRKY genes responded strongly to drought and salt stress, which provide candidate ZjWRKY genes for facilitating tolerance breeding.

scholarly journals Genome-Wide Identification and Analysis of the WRKY Gene Family and Cold Stress Response in Acer truncatum

Genes ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1867
Author(s):  
Yan Li ◽  
Xiang Li ◽  
Jiatong Wei ◽  
Kewei Cai ◽  
Hongzhi Zhang ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Stress Response ◽  
Cold Stress ◽  
Gene Family ◽  
Gene Families ◽  
Regulatory Function ◽  
Wrky Gene ◽  
Wrky Transcription Factors ◽  
Biotic Stresses ◽  
Genome Wide

WRKY transcription factors constitute one of the largest gene families in plants and are involved in many biological processes, including growth and development, physiological metabolism, and the stress response. In earlier studies, the WRKY gene family of proteins has been extensively studied and analyzed in many plant species. However, information on WRKY transcription factors in Acer truncatum has not been reported. In this study, we conducted genome-wide identification and analysis of the WRKY gene family in A. truncatum, 54 WRKY genes were unevenly located on all 13 chromosomes of A. truncatum, the highest number was found in chromosomes 5. Phylogenetic relationships, gene structure, and conserved motif identification were constructed, and the results affirmed 54 AtruWRKY genes were divided into nine subgroup groups. Tissue species analysis of AtruWRKY genes revealed which were differently exhibited upregulation in flower, leaf, root, seed and stem, and the upregulation number were 23, 14, 34, 18, and 8, respectively. In addition, the WRKY genes expression in leaf under cold stress showed that more genes were significantly expressed under 0, 6 and 12 h cold stress. The results of this study provide a new insight the regulatory function of WRKY genes under abiotic and biotic stresses.

scholarly journals Genome wide analysis of stress responsive WRKY transcription factors in Arabidopsis thaliana

2016 ◽  
Vol 4 (4) ◽  
pp. 279 ◽  
Author(s):  
Shaiq Sultan ◽  
Muhammad Amjid Ali ◽  
Rana Muhammad Atif ◽  
Farrukh Azeem ◽  
Habibullah Nadeem ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factors ◽  
Chromosomal Localization ◽  
Evolutionary Relationship ◽  
Wrky Transcription Factors ◽  
Specific Sequence ◽  
Multiple Sequence ◽  
Genome Wide Analysis ◽  
Functional Homology ◽  
Genome Wide

WRKY transcription factors are a class of DNA-binding proteins that bind with a specific sequence C/TTGACT/C known as W-Box found in promoters of genes which are regulated by these WRKYs. From previous studies, 43 different stress responsive WRKY transcription factors in Arabidopsis thaliana, identified and then categorized in three groups viz., abiotic, biotic and both of these stresses. A comprehensive genome wide analysis including chromosomal localization, gene structure analysis, multiple sequence alignment, phylogenetic analysis and promoter analysis of these WRKY genes was carried out in this study to determine the functional homology in Arabidopsis. This analysis led to the classification of these WRKY family members into 3 major groups and subgroups and showed evolutionary relationship among these groups on the base of their functional WRKY domain, chromosomal localization and intron/exon structure. The proposed groups of these stress responsive WRKY genes and annotation based on their position on chromosomes can also be explored to determine their functional homology in other plant species in relation to different stresses. The result of the present study provides indispensable genomic information for the stress responsive WRKY transcription factors in Arabidopsis and will pave the way to explain the precise role of various AtWRKYs in plant growth and development under stressed conditions.

scholarly journals Genome-wide identification of MAPKKK genes and their responses to phytoplasma infection in Chinese jujube (Ziziphus jujuba Mill.)

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Zhiguo Liu ◽  
Lixin Wang ◽  
Chaoling Xue ◽  
Yuetong Chu ◽  
Weilin Gao ◽  
...  
Keyword(s):  
Chinese Jujube ◽  
Genome Wide ◽  
Phytoplasma Infection ◽  
Ziziphus Jujuba

Genome-wide identification, characterization, and expression analysis of the expansin gene family in Chinese jujube (Ziziphus jujuba Mill.)

Planta ◽  
2018 ◽  
Vol 249 (3) ◽  
pp. 815-829 ◽  
Author(s):  
Lu Hou ◽  
Zhiyong Zhang ◽  
Suhan Dou ◽  
Yadong Zhang ◽  
Xiaoming Pang ◽  
...  
Keyword(s):  
Gene Family ◽  
Expression Analysis ◽  
Chinese Jujube ◽  
Expansin Gene ◽  
Genome Wide ◽  
Ziziphus Jujuba

scholarly journals Genome-wide analysis of WRKY transcription factors in Aquilaria sinensis (Lour.) Gilg

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yan-Hong Xu ◽  
Pei-Wen Sun ◽  
Xiao-Lin Tang ◽  
Zhi-Hui Gao ◽  
Zheng Zhang ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Wrky Transcription Factors ◽  
Aquilaria Sinensis ◽  
Genome Wide Analysis ◽  
Genome Wide

scholarly journals Genome-Wide Organization and Expression Profiling of the SBP-Box Gene Family in Chinese Jujube (Ziziphus jujuba Mill.)

2017 ◽  
Vol 18 (8) ◽  
pp. 1734 ◽  
Author(s):  
Shuang Song ◽  
Heying Zhou ◽  
Songbai Sheng ◽  
Ming Cao ◽  
Yingyue Li ◽  
...  
Keyword(s):  
Gene Family ◽  
Expression Profiling ◽  
Chinese Jujube ◽  
Genome Wide ◽  
Ziziphus Jujuba

scholarly journals Genome-Wide Characterization of R2R3-MYB Transcription Factors in Pitaya Reveals a R2R3-MYB Repressor HuMYB1 Involved in Fruit Ripening through Regulation of Betalain Biosynthesis by Repressing Betalain Biosynthesis-Related Genes

Cells ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1949
Author(s):  
Fangfang Xie ◽  
Qingzhu Hua ◽  
Canbin Chen ◽  
Zhike Zhang ◽  
Rong Zhang ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Gene Family ◽  
Fruit Ripening ◽  
Phylogenetic Analyses ◽  
Luciferase Reporter ◽  
Molecular Characteristics ◽  
Genome Data ◽  
Genome Wide ◽  
Hylocereus Undatus ◽  
R2r3 Myb

The MYB (myeloblastosis) superfamily constitutes one of the most abundant transcription factors (TFs) regulating various biological processes in plants. However, the molecular characteristics and functions of MYB TFs in pitaya remain unclear. To date, no genome-wide characterization analysis of this gene family has been conducted in the Cactaceae species. In this study, 105 R2R3-MYB members were identified from the genome data of Hylocereus undatus and their conserved motifs, physiological and biochemical characteristics, chromosome locations, synteny relationship, gene structure and phylogeny were further analyzed. Expression analyses suggested that three up-regulated HuMYBs and twenty-two down-regulated HuMYBs were probably involved in fruit ripening of pitaya. Phylogenetic analyses of R2R3-MYB repressors showed that seven HuMYBs (HuMYB1, HuMYB21, HuMYB48, HuMYB49, HuMYB72, HuMYB78 and HuMYB101) were in clades containing R2R3-MYB repressors. HuMYB1 and HuMYB21 were significantly down-regulated with the betalain accumulation during fruit ripening of ‘Guanhuahong’ pitaya (H. monacanthus). However, only HuMYB1 had R2 and R3 repeats with C1, C2, C3 and C4 motifs. HuMYB1 was localized exclusively to the nucleus and exhibited transcriptional inhibition capacities. Dual luciferase reporter assay demonstrated that HuMYB1 inhibited the expression of betalain-related genes: HuADH1, HuCYP76AD1-1 and HuDODA1. These results suggested that HuMYB1 is a potential repressor of betalain biosynthesis during pitaya fruit ripening. Our results provide the first genome-wide analyses of the R2R3-MYB subfamily involved in pitaya betalain biosynthesis and will facilitate functional analysis of this gene family in the future.

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