scholarly journals Screening and Molecular Identification of Novel Pectinolytic Bacteria from Forest Soil

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 40
Author(s):  
Sarita Shrestha ◽  
Janak Raj Khatiwada ◽  
Xiaodong Zhang ◽  
Chonlong Chio ◽  
Aristide Laurel Mokale Kognou ◽  
...  
Keyword(s):  
Forest Soil ◽  
Fruit Juice ◽  
Xylanase Activity ◽  
Plant Fiber ◽  
Sesame Seeds ◽  
Rdna Sequencing ◽  
Juice Extraction ◽  
Pectinase Activity ◽  
Industrial Demand ◽  
Fiber Processing

Pectinases are a group of enzymes with broad application, including in plant fiber processing, pectic wastewater treatment, paper pulping, fruit juice extraction, and clarification. With an increasing industrial demand for these enzymes, it is useful to isolate organisms that produce large amounts of pectinase and possess wide ranges of stability factors like temperature and pH. In this study, 17 out of 29 bacteria (58.62%) from forest soil samples were pectinolytic. However, only four bacteria (S-5, S-10, S-14, and S-17) showed high pectin hydrolysis zones (ranging from 0.2 cm to 1.7 cm). These four bacteria were identified based on colony morphology, microscopic characterization, biochemical characteristics, and 16S rDNA sequencing. They were designated as Streptomyces sp. (S-5, S-14), Cellulomonas sp. (S-10), and Bacillus sp. (S-17). Interestingly, bacteria showed cellulase and xylanase activity in addition to pectinase. The quantitative assay for pectinase activity of the four isolates provided proof that they are pectinase producers and can be considered potential candidates for industrial uses. The crude enzyme extracts of these bacteria are applicable in oil and juice extraction from sesame seeds and apples, respectively.

scholarly journals Screening of a Novel Polysaccharide Lyase Family 10 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression and Characterization

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2774 ◽  
Author(s):  
Yan Zhao ◽  
Ye Yuan ◽  
Xinyu Zhang ◽  
Yumei Li ◽  
Qiang Li ◽  
...  
Keyword(s):  
Textile Industry ◽  
Pectate Lyase ◽  
Paenibacillus Polymyxa ◽  
Polygalacturonic Acid ◽  
Optimal Conditions ◽  
Citrus Pectin ◽  
Plant Fiber ◽  
Polysaccharide Lyase ◽  
Glycosidic Bonds ◽  
Fiber Processing

Pectate lyase (EC 4.2.2.2) catalyzes the cleavage of α-1,4-glycosidic bonds of pectin polymers, and it has potential uses in the textile industry. In this study, a novel pectate lyase belonging to polysaccharide lyase family 10 was screened from the secreted enzyme extract of Paenibacillus polymyxa KF-1 and identified by liquid chromatography-MS/MS. The gene was cloned from P. polymyxa KF-1 genomic DNA and expressed in Escherichia coli. The recombinant enzyme PpPel10a had a predicted Mr of 45.2 kDa and pI of 9.41. Using polygalacturonic acid (PGA) as substrate, the optimal conditions for PpPel10a reaction were determined to be 50 °C and pH 9.0, respectively. The Km, vmax and kcat values of PpPel10a with PGA as substrate were 0.12 g/L, 289 μmol/min/mg, and 202.3 s−1, respectively. Recombinant PpPel10a degraded citrus pectin, producing unsaturated mono- and oligogalacturonic acids. PpPel10a reduced the viscosity of PGA, and weight loss of ramie (Boehmeria nivea) fibers was observed after treatment with the enzyme alone (22.5%) or the enzyme in combination with alkali (26.3%). This enzyme has potential for use in plant fiber processing.

Gamma Irradiation Usage in Fruit Juice Extraction

Fruit Juices ◽  
2018 ◽  
pp. 423-435 ◽  
Author(s):  
Ratish Ramanan Kalaiselvan ◽  
Anandakumar Sugumar ◽  
Mahendran Radhakrishnan
Keyword(s):  
Gamma Irradiation ◽  
Fruit Juice ◽  
Juice Extraction

scholarly journals Pectin Degradation in Fruit Juices by Pectinase from Meyerozyma sp. VITPCT75 Isolated from Phyllanthus emblica

2021 ◽  
Author(s):  
B. Meena ◽  
V.G. Sowmeya ◽  
Archa B. Praveen ◽  
A. Swetha ◽  
D. Naga Sarath Chandra ◽  
...  
Keyword(s):  
Metal Ion ◽  
Storage Stability ◽  
Fruit Juice ◽  
Partial Purification ◽  
Phyllanthus Emblica ◽  
Pectin Degradation ◽  
Juice Clarification ◽  
Pectinase Activity ◽  
Pectinase Production ◽  
Fruit Juice Clarification

This study aimed to identify and characterize a pectinase-producing novel yeast from the fermented juice of Phyllanthus emblica and apply the enzyme for fruit juice clarification. Among the five pectinase-producing yeasts, isolate-1 exhibited the highest pectinase activity and was further used in this study. Based on morphological, physiological, and 18SrRNAanalyses, isolate-1 was recognized as a new strain sharing 99% sequence homology with other Meyerozyma strains and was thus designated as Meyerozyma sp. VITPCT75. The strain produced pectinase optimally at a temperature and pH of 25oC and 7, respectively. Maximum pectinase production was observed after 4-days incubation. The enzyme exhibited optimum activity at the temperature of 25 °C and pH 7.0. The enzyme was more stable at a temperature and pH of 20 °C and 7, respectively. Storage stability studies revealed that the enzyme was stable at -20 °C. The cell-free supernatant was partially purified using ammonium sulfate and solvent precipitation. Acetone at a concentration of 20% assured an adequate partial purification. The molecular weight of pectinase was determined as 6 kDa. The enzymatic metal ion preference-related studies revealed that Ca²z, Kz, Cu²z, Fe²z, and Ba²z ions enhanced, Ni²z ions moderately inhibited, and Mn²z ions intensely inhibited the enzymatic activity. Neither Na+ and Mg2+ ions nor EDTA affected the enzyme activity. When subjected to fruit juice clarification, the enzyme significantly reduced the viscosity of the juice.

scholarly journals Isolation of thermotolerant Bacillus subtilis DCH4 from Chancos hot spring (Carhuaz, Peru) with potential to degrade lignocellulosic agriculture wastes

2020 ◽  
Vol 27 (1) ◽  
pp. 067-078
Author(s):  
Carmen Tamariz-Angeles ◽  
Jasmine Lázaro-Palomino ◽  
Percy Olivera-Gonzales ◽  
Alberto Castañeda-Barreto ◽  
Gretty K. Villena
Keyword(s):  
Bacillus Subtilis ◽  
Hot Spring ◽  
Xylanase Activity ◽  
Biofuel Production ◽  
Ex Situ ◽  
Sequencing Analysis ◽  
Endoglucanase Activity ◽  
Rdna Sequencing ◽  
Hydrolytic Activities ◽  
Domain 3

It was isolated bacteria strains from three different types of samples: fresh water, in situ baits and ex situ enrichment. Serial dilutions were prepared and culture was carried at 50 °C using a Basal-Saline medium. Isolated strains were screened for endoglucanase and xylanase activities with qualitative (Congo Red) and quantitative (DNS) methods. Molecular 16S rDNA sequencing analysis was performed for taxonomic identification. It was isolated 31 strains of which 14 showed hydrolytic activities and belonged to Bacillus subtilis and Bacillus licheniformis species. Moreover, the strain B. subtilis DCH4 showed the highest endoglucanase activity at 45°C and pH 5, and xylanase activity at 55°C and pH 6. Then, DCH4 was cultivated by submerged fermentation with two different media supplemented with sugar cane bagasse, wheat straw, or quinoa stalk to evaluate its saccharification capability. Likewise, it was screening its xylanase and cellulase genes employing specific primers; the amplicons obtained were sequenced, and analyzed. It was found that, enzymatic extracts of DCH4 prepared with cane bagasse or quinoa stalk media achieved the highest endoglucanase and xylanase activities. According to molecular analysis of genes involved in the hydrolytic process, the endoglucanase and xylanase activities exhibited by DCH4 could be attributed to a bifunctional cellulase conformed by endo-beta-1,4-glucanase (GH5) joined to cellulose binding domain 3 (CBM3), and an endo-1,4-beta-xylanase (GH11), respectively. Further transcriptomic experiments would be considered to accomplish optimization strategies for biofuel production from lignocellulosic biomass.

Use of Fruit Juice in the Diets of Young Children

PEDIATRICS ◽  
1995 ◽  
Vol 95 (3) ◽  
pp. 433-433
Author(s):  
William J. Klish
Keyword(s):  
United States ◽  
Young Children ◽  
Fruit Juice ◽  
The United States ◽  
Information Resources ◽  
Adequate Supply ◽  
The Past ◽  
Juice Extraction ◽  
Excessive Consumption

Fruit juice is a modern addition to the infant's diet due to the development of technology for juice extraction and an adequate supply of fruit. It has been marketed as healthy, natural, and nutritious. As a result, parents (in trying to do what is best for their infants) have been feeding juices in increasing quantities. In 1992, $164 million was spent on bottled baby juice in the United States according to Information Resources, Inc, a marketing company that tracks the consumer industry. For the past several decades, pediatricians have recognized that excessive consumption of fruit juice has been associated with diarrhea, gassiness, stomachaches, and other abdominal complaints.

Economic efficiency and investment potential in the smallholder crop sector in Malawi

2000 ◽  
Vol 27 (7/8/9/10) ◽  
pp. 968-979 ◽  
Author(s):  
Julius H. Mangisoni
Keyword(s):  
Crop Production ◽  
Net Present Value ◽  
Fruit Juice ◽  
Financial Analysis ◽  
Cost Ratio ◽  
Full Potential ◽  
Extraction Plant ◽  
Benefit Cost Ratio ◽  
Juice Extraction ◽  
Benefit Cost

The crop sector in Malawi faces a number of constraints which must be overcome before agriculture can reach its full potential. This paper uses the policy analysis matrix (PAM) to assess efficiency in the crop sector and financial analysis to explore the potential of investing in a fruit juice extraction plant in Malawi. The PAM revealed that farmers in Malawi are efficient producers but they face negative incentives in the production and marketing of their products. The nominal protection coefficients (NPCs) for both maize and beans were less than 1 while those of inputs were more than 1. Similarly, the effective protection coefficients (EPCs) were less than 1, implying that the combined effect of transfers and tradable inputs is reducing the private profitability of the systems. On the other hand, the financial analysis demonstrated that Malawi can sustain a fruit juice extraction plant. The project had a positive net present value and a benefit/cost ratio greater than 1 (1.16). Recommendations made from the study relate to the need for diversification into fruits that can sustain a fruit juice extraction plant, encouraging private sector participation and reducing disincentives to crop production.

Sign in / Sign up

Export Citation Format

Share Document