scholarly journals Bioethanol Production from Food Waste Applying the Multienzyme System Produced On-Site by Fusarium oxysporum F3 and Mixed Microbial Cultures

Fermentation ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 39 ◽  
Author(s):  
George Prasoulas ◽  
Aggelos Gentikis ◽  
Aikaterini Konti ◽  
Styliani Kalantzi ◽  
Dimitris Kekos ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Ethanol Production ◽  
Mixed Culture ◽  
Food Waste ◽  
Bioethanol Production ◽  
Microbial Cultures ◽  
Multienzyme System ◽  
Mixed Microbial Cultures ◽  
The Cost ◽  
Hydrolysis Of

Waste management and production of clean and affordable energy are two main challenges that our societies face. Food waste (FW), in particular, can be used as a feedstock for the production of ethanol because of its composition which is rich in cellulose, hemicellulose and starch. However, the cost of the necessary enzymes used to convert FW to ethanol remains an obstacle. The on-site production of the necessary enzymes could be a possible solution. In the present study, the multienzyme production by the fungus Fusarium oxysporum F3 under solid state cultivation using different agroindustrial residues was explored. Maximum amylase, glucoamylase, endoglucanase, b-glucosidase, cellobiohydrolase, xylanase, b-xylosidase and total cellulase titers on wheat bran (WB) were 17.8, 0.1, 65.2, 27.4, 3.5, 221.5, 0.7, 0.052 and 1.5 U/g WB respectively. F. oxysporum was used for the hydrolysis of FW and the subsequent ethanol production. To boost ethanol production, mixed F. oxysporum and S. cerevisiae cultures were also used. Bioethanol production by F. oxysporum monoculture reached 16.3 g/L (productivity 0.17 g/L/h), while that of the mixed culture was 20.6 g/L (productivity 1.0 g/L/h). Supplementation of the mixed culture with glucoamylase resulted in 30.3 g/L ethanol with a volumetric productivity of 1.4 g/L/h.

Production of poly-3-hydroxybutyrate from mixed culture

Biologia ◽  
2016 ◽  
Vol 71 (7) ◽  
Author(s):  
Thomas Shalin ◽  
Raveendran Sindhu ◽  
Ashok Pandey ◽  
Vincenza Faraco ◽  
Parameswaran Binod
Keyword(s):  
Mixed Culture ◽  
Biodegradable Polymers ◽  
Biodegradable Polymer ◽  
The Other ◽  
Cost Difference ◽  
Microbial Cultures ◽  
Phb Production ◽  
Mixed Microbial Cultures ◽  
Main Barrier ◽  
The Cost

AbstractPoly-3-hydroxybutyrate (PHB) is a biodegradable polymer produced by many bacteria. Some of the properties are similar to thermoplastics like polypropylene, hence finding application PHB can directly replace non-biodegradable polymers. But the main barrier has been the cost difference. The utilization of mixed microbial cultures facilitates the use of complex substrates and thus can reduce the cost of PHB production. In the present study, mixed culture systems, where metabolite produced by one organism may be assimilated by the other organism, were employed.

Key drivers influencing the commercialization of ethanol-based biorefineries

1969 ◽  
Vol 16 (3) ◽  
Author(s):  
Anuj K Chandel ◽  
Om V Singh ◽  
Gajula Chandrasekhar ◽  
Linga Venkateswar Rao ◽  
Mangamoori Lakshmi Narasu
Keyword(s):  
Ethanol Production ◽  
Large Scale ◽  
Fossil Fuels ◽  
Process Integration ◽  
Raw Materials ◽  
Cellulase Production ◽  
Bioethanol Production ◽  
Recent Developments ◽  
Key Drivers ◽  
The Cost

The imposition of ethanol derived from biomass for blending in gasoline would make countries less dependent on current petroleum sources, which would save foreign exchange reserves, improve rural economies and provide job opportunities in a clean and safe environment. The key drivers for successful commercial ethanol production are cheap raw materials, economic pretreatment technologies, in-house cellulase production with high and efficient titers, high ethanol fermentation rates, downstream recovery of ethanol and maximum by-products utilization. Furthermore, recent developments in engineering of biomass for increased biomass, down-regulation of lignin synthesis, improved cellulase titers and re-engineering of cellulases, and process integration of the steps involved have increased the possibility of cheap bioethanol production that competes with the price of petroleum. Recently, many companies have come forward globally for bioethanol production on a large scale. It is very clear now that bioethanol will be available at the price of fossil fuels by 2010. This article intends to provide insight and perspectives on the important recent developments in bioethanol research, the commercialization status of bioethanol production, the step-wise cost incurred in the process involved, and the possible innovations that can be utilized to reduce the cost of ethanol production.

scholarly journals From Food Waste to Volatile Fatty Acids towards a Circular Economy

2021 ◽  
Author(s):  
Mónica Carvalheira ◽  
Anouk F. Duque
Keyword(s):  
Fatty Acids ◽  
Food Waste ◽  
Circular Economy ◽  
Volatile Fatty Acids ◽  
Animal Feed ◽  
Anaerobic Fermentation ◽  
Industrial Sector ◽  
Added Value ◽  
Microbial Cultures ◽  
Mixed Microbial Cultures

The food industrial sector generates large amounts of waste, which are often used for animal feed, for agriculture or landfilled. However, these wastes have a very reach composition in carbon and other compounds, which make them very attractive for valorization through biotechnological processes. Added value compounds, such as volatile fatty acids (VFAs), can be produced by anaerobic fermentation using pure cultures or mixed microbial cultures and food waste as carbon source. Research on valuable applications for VFAs, such as polyhydroxyalkanoates, bioenergy or biological nutrient removal, towards a circular economy is emerging. This enhances the sustainability and the economic value of food waste. This chapter reviews the various types of food waste used for VFAs production using mixed microbial cultures, the anaerobic processes, involved and the main applications for the produced VFAs. The main parameters affecting VFAs production are also discussed.

scholarly journals Direct Ethanol Production from Lignocellulosic Materials by Mixed Culture of Wood Rot Fungi Schizophyllum commune, Bjerkandera adusta, and Fomitopsis palustris

Fermentation ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 21 ◽  
Author(s):  
Sakae Horisawa ◽  
Akie Inoue ◽  
Yuka Yamanaka
Keyword(s):  
Ethanol Production ◽  
Mixed Culture ◽  
Consolidated Bioprocessing ◽  
Schizophyllum Commune ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignocellulosic Materials ◽  
Bjerkandera Adusta ◽  
Fomitopsis Palustris ◽  
The Cost

The cost of bioethanol production from lignocellulosic materials is relatively high because the additional processes of delignification and saccharification are required. Consolidated bioprocessing (CBP) simultaneously uses the multiple processes of delignification, saccharification, and fermentation in a single reactor and has the potential to solve the problem of cost. Some wood-degrading basidiomycetes have lignin- and cellulose-degrading abilities as well as ethanol fermentation ability. The white rot fungus Schizophyllum commune NBRC 4928 was selected as a strong fermenter from a previous study. The lignin-degrading fungus Bjerkandera adusta and polysaccharide-degrading fungus Fomitopsis palustris were respectively added to S. commune ethanol fermentations to help degrade lignocellulosic materials. Bjerkandera adusta produced more ligninase under aerobic conditions, so a switching aeration condition was adopted. The mixed culture of S. commune and B. adusta promoted direct ethanol production from cedar wood. Fomitopsis palustris produced enzymes that released glucose from both carboxymethylcellulose and microcrystalline cellulose. The mixed culture of S. commune and F. palustris did not enhance ethanol production from cedar. The combination of S. commune and cellulase significantly increased the rate of ethanol production. The results suggest that CBP for ethanol production from cellulosic material can be achieved by using multiple fungi in one reactor.

scholarly journals Designing Efficient Processes for Sustainable Bioethanol and Bio-Hydrogen Production from Grass Lawn Waste

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2889
Author(s):  
Georgia Antonopoulou
Keyword(s):  
Simultaneous Saccharification And Fermentation ◽  
Pichia Stipitis ◽  
Bioethanol Production ◽  
Alkali Pretreatment ◽  
Pachysolen Tannophilus ◽  
Microbial Cultures ◽  
Separate Hydrolysis And Fermentation ◽  
Mixed Microbial Cultures ◽  
Pretreatment Conditions ◽  
Simultaneous Saccharification

The effect of thermal, acid and alkali pretreatment methods on biological hydrogen (BHP) and bioethanol production (BP) from grass lawn (GL) waste was investigated, under different process schemes. BHP from the whole pretreatment slurry of GL was performed through mixed microbial cultures in simultaneous saccharification and fermentation (SSF) mode, while BP was carried out through the C5yeast Pichia stipitis, in SSF mode. From these experiments, the best pretreatment conditions were determined and the efficiencies for each process were assessed and compared, when using either the whole pretreatment slurry or the separated fractions (solid and liquid), the separate hydrolysis and fermentation (SHF) or SSF mode, and especially for BP, the use of other yeasts such as Pachysolen tannophilus or Saccharomyces cerevisiae. The experimental results showed that pretreatment with 10 gH2SO4/100 g total solids (TS) was the optimum for both BHP and BP. Separation of solid and liquid pretreated fractions led to the highest BHP (270.1 mL H2/g TS, corresponding to 3.4 MJ/kg TS) and also BP (108.8 mg ethanol/g TS, corresponding to 2.9 MJ/kg TS) yields. The latter was achieved by using P. stipitis for the fermentation of the hydrolysate and S. serevisiae for the solid fraction fermentation, at SSF.

Enzymatical hydrolysis of food waste and ethanol production from the hydrolysate

2011 ◽  
Vol 36 (4) ◽  
pp. 1259-1265 ◽  
Author(s):  
Shoubao Yan ◽  
Jun Li ◽  
Xiangsong Chen ◽  
Jingyong Wu ◽  
Pingchao Wang ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Food Waste ◽  
Hydrolysis Of

scholarly journals Assessment of Long-Term Fermentability of PHA-Based Materials from Pure and Mixed Microbial Cultures for Potential Environmental Applications

Water ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 897
Author(s):  
Neda Amanat ◽  
Bruna Matturro ◽  
Marta Maria Rossi ◽  
Francesco Valentino ◽  
Marianna Villano ◽  
...  
Keyword(s):  
Environmental Remediation ◽  
Volatile Fatty Acids ◽  
Rapid Onset ◽  
Pilot Scale ◽  
Point Of View ◽  
Microbial Cultures ◽  
Extraction And Purification ◽  
Fermentation Pattern ◽  
Mixed Microbial Cultures

The use of polyhydroxyalkanoates (PHA) as slow-release electron donors for environmental remediation represents a novel and appealing application that is attracting considerable attention in the scientific community. In this context, here, the fermentation pattern of different types of PHA-based materials has been investigated in batch and continuous-flow experiments. Along with commercially available materials, produced from axenic microbial cultures, PHA produced at pilot scale by mixed microbial cultures (MMC) using waste feedstock have been also tested. As a main finding, a rapid onset of volatile fatty acids (VFA) production was observed with a low-purity MMC-deriving material, consisting of microbial cells containing 56% (on weight basis) of intracellular PHA. Indeed, with this material a sustained, long-term production of organic acids (i.e., acetic, propionic, and butyric acids) was observed. In addition, the obtained yield of conversion into acids (up to 70% gVFA/gPHA) was higher than that obtained with the other tested materials, made of extracted and purified PHA. These results clearly suggest the possibility to directly use the PHA-rich cells deriving from the MMC production process, with no need of extraction and purification procedures, as a sustainable and effective carbon source bringing remarkable advantages from an economic and environmental point of view.

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