scholarly journals Genetic Diversity and Structure of Pinus densiflora Siebold & Zucc. Populations in Republic of Korea Based on Microsatellite Markers

Forests ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 750
Author(s):  
Ji-Young Ahn ◽  
Jei-Wan Lee ◽  
Kyung-Nak Hong
Keyword(s):  
Genetic Diversity ◽  
Genetic Differentiation ◽  
Microsatellite Markers ◽  
Environmental Changes ◽  
Pinus Densiflora ◽  
Republic Of Korea ◽  
Conifer Species ◽  
Principal Coordinates ◽  
Genetic Diversity And Structure ◽  
The Republic

Pinus densiflora Siebold & Zucc. is a widely distributed conifer species in the Republic of Korea with economic and ecologic importance. However, P. densiflora is negatively influenced by various factors, such as forest fires, clearing of large numbers of trees infected with Pinus disease, and dieback. We aimed to use microsatellite markers to estimate the genetic diversity, differentiation, and structure of P. densiflora populations in the Republic of Korea. A total of 1844 samples from 60 populations were evaluated using 11 polymorphic microsatellite markers. The observed heterozygosity and expected heterozygosity were 0.652 and 0.673, respectively. The mean genetic differentiation among the populations was 0.013. Moreover, P. densiflora showed high genetic diversity and low genetic differentiation compared with conifer species, including Pinus species with similar life histories. Principal coordinates analysis and Bayesian clustering showed that P. densiflora has a weak geographical structure. The P. densiflora population at Mt. Halla, Jeju Island, showed the lowest genetic diversity and significant genetic differentiation compared with other mainland populations due to genetic drift and restricted gene flow. These findings can be useful for designing new conservation, management, and breeding strategies for P. densiflora populations in response to future environmental changes.

scholarly journals Genetic Structure and Eco-Geographical Differentiation of Lancea tibetica in the Qinghai-Tibetan Plateau

Genes ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 97 ◽  
Author(s):  
Xiaofeng Chi ◽  
Faqi Zhang ◽  
Qingbo Gao ◽  
Rui Xing ◽  
Shilong Chen
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Gene Flow ◽  
Tibetan Plateau ◽  
Environmental Changes ◽  
Restricted Gene Flow ◽  
Principal Coordinates Analysis ◽  
Principal Coordinates ◽  
Genetic Diversity And Structure ◽  
Tanggula Mountains

The uplift of the Qinghai-Tibetan Plateau (QTP) had a profound impact on the plant speciation rate and genetic diversity. High genetic diversity ensures that species can survive and adapt in the face of geographical and environmental changes. The Tanggula Mountains, located in the central of the QTP, have unique geographical significance. The aim of this study was to investigate the effect of the Tanggula Mountains as a geographical barrier on plant genetic diversity and structure by using Lancea tibetica. A total of 456 individuals from 31 populations were analyzed using eight pairs of microsatellite makers. The total number of alleles was 55 and the number per locus ranged from 3 to 11 with an average of 6.875. The polymorphism information content (PIC) values ranged from 0.2693 to 0.7761 with an average of 0.4378 indicating that the eight microsatellite makers were efficient for distinguishing genotypes. Furthermore, the observed heterozygosity (Ho), the expected heterozygosity (He), and the Shannon information index (I) were 0.5277, 0.4949, and 0.9394, respectively, which indicated a high level of genetic diversity. We detected high genetic differentiation among all sampling sites and restricted gene flow among populations. Bayesian-based cluster analysis (STRUCTURE), principal coordinates analysis (PCoA), and Neighbor-Joining (NJ) cluster analysis based on microsatellite markers grouped the populations into two clusters: the southern branch and the northern branch. The analysis also detected genetic barriers and restricted gene flow between the two groups separated by the Tanggula Mountains. This study indicates that the geographical isolation of the Tanggula Mountains restricted the genetic connection and the distinct niches on the two sides of the mountains increased the intraspecific divergence of the plants.

scholarly journals The Relationship between Tree-Ring Growths of Pinus densiflora and Climate from Three Mountains in Central Region, the Republic of Korea

Atmosphere ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 878
Author(s):  
Chang-Hyun Park ◽  
Ui-Cheon Lee ◽  
Soo-Chul Kim ◽  
Kwang-Hee Lee
Keyword(s):  
Tree Ring ◽  
National Parks ◽  
Central Region ◽  
Climatic Factors ◽  
Pinus Densiflora ◽  
Ring Width ◽  
Republic Of Korea ◽  
Logarithmic Curve ◽  
The Republic ◽  
The Relationship

To analyze the relationship between climatic factors (monthly mean temperature and total precipitation) and tree-ring growths of Pinus densiflora from the central region of the Republic of Korea, more than 20 trees were sampled from three national parks. The tree-ring chronology of Mt. Bukhan covering the period of 1917–2016 was assessed, as well as that of Mt. Seorak across 1687–2017 and Mt. Worak across 1777–2017. After cross-dating, each ring-width series was double-standardized by first fitting a logarithmic curve and then a 50 year cubic spline. Climate-growth relationships were computed with bootstrap correlation functions. The result of the analysis showed a positive response from the current March temperature and May precipitations for tree-ring growth of Pinus densiflora. It indicates that a higher temperature supply during early spring season and precipitation during cambium activity are important for radial growths of Pinus densiflora from the central region in the Republic of Korea.

scholarly journals Revelation of genetic diversity and structure of wild Elymus excelsus (Poaceae: Triticeae) collection from western China by SSR markers

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e8038
Author(s):  
Yanli Xiong ◽  
Wenhui Liu ◽  
Yi Xiong ◽  
Qingqing Yu ◽  
Xiao Ma ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Western China ◽  
Tibet Plateau ◽  
Group Method ◽  
Breeding Strategies ◽  
Population Genetic Differentiation ◽  
Principal Coordinates ◽  
Geographical Regions ◽  
Genetic Diversity And Structure

Hosting unique and important plant germplasms, the Qinghai-Tibet Plateau (QTP), as the third pole of the world, and Xinjiang, located in the centre of the Eurasian continent, are major distribution areas of perennial Triticeae grasses, especially the widespread Elymus species. Elymus excelsus Turcz. ex Griseb, a perennial forage grass with strong tolerance to environmental stresses, such as drought, cold and soil impoverishment, can be appropriately used for grassland establishment due to its high seed production. To provide basic information for collection, breeding strategies and utilization of E. excelsus germplasm, microsatellite markers (SSR) were employed in the present study to determine the genetic variation and population structure of 25 wild accessions of E. excelsus from Xinjiang (XJC) and the QTP, including Sichuan (SCC) and Gansu (GSC) of western China. Based on the 159 polymorphic bands amplified by 35 primer pairs developed from three related species, the average values of the polymorphic information content (PIC), marker index (MI), resolving power (Rp), Nei’s genetic diversity (H) and Shannon’s diversity index (I) of each pair of primers were 0.289, 1.348, 1.897, 0.301 and 0.459, respectively, validating that these SSR markers can also be used for the evaluation of genetic diversity of E. excelsus germplasms, and demonstrating the superior versatility of EST-SSR vs. G-SSR. We found a relatively moderate differentiation (Fst = 0.151) among the XJC, SCC and GSC geo-groups, and it is worth noting that, the intra-group genetic diversity of the SCC group (He = 0.197) was greater than that of the GSC (He = 0.176) and XJC (He = 0.148) groups. Both the Unweighted Pair Group Method with Arithmetic (UPGMA) clustering and principal coordinates analysis (PCoA) divided the 25 accessions into three groups, whereas the Bayesian STRUCTURE analysis suggested that E. excelsus accessions fell into four main clusters. Besides, this study suggested that geographical distance and environmental variables (annual mean precipitation and average precipitation in growing seasons), especially for QTP accessions, should be combined to explain the population genetic differentiation among the divergent geographical regions. These data provided comprehensive information about these valuable E. excelsus germplasm resources for the protection and collection of germplasms and for breeding strategies in areas of Xinjiang and QTP in western China.

scholarly journals Study of the gene pool of the winter rye Secale cereale L. of the Republic of Belarus using microsatellite markers

2021 ◽  
Vol 66 (2) ◽  
pp. 215-222
Author(s):  
V. S. Mandrusova ◽  
I. S. Gordej ◽  
O. M. Lyusikov ◽  
V. E. Shimko ◽  
I. A. Gordej
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Gene Pool ◽  
Winter Rye ◽  
Interpopulation Variability ◽  
Content Index ◽  
Secale Cereale L ◽  
New Varieties ◽  
The Republic

In this work, the genetic diversity of the modern gene pool of the winter rye (S. cereal L.) of the Republic of Belarus from 20 actual breeding samples was investigated using 15 microsatellite (SSR) markers to develop divergent crossing combinations in breeding for heterosis. It was shown that the formed set of SSR markers is highly effective – the informational content index (PIC) varied from 0.50 to 0.83 and averaged 0.72. The most effective microsatellite markers (SCM28, SCM43, SCM101 and SCM102) were identified and can be successfully used to study the genetic diversity of rye. It has been established that the modern gene pool of the winter rye of the Republic of Belarus is generally characterized by fairly wide genetic diversity (interpopulation variability) – all collection samples are characterized by a unique allelic composition of the studied microsatellite loci. Based on investigation results, a hierarchical clustering dendrogram was constructed, which made it possible to determine the most genetically divergent combinations of crosses. The information obtained can be used for the development of an effective scheme allowing to develop new varieties and hybrids in the practical breeding of rye for heterosis.

scholarly journals Genetic Diversity and Structure of Latvian Tilia cordata Populations

2021 ◽  
Vol 75 (4) ◽  
pp. 261-267
Author(s):  
Dainis Edgars Ruņģis ◽  
Baiba Krivmane
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Vegetative Reproduction ◽  
Growth Conditions ◽  
Climatic Conditions ◽  
Tilia Cordata ◽  
High Genetic Diversity ◽  
Genetic Diversity And Structure ◽  
Broadleaf Tree ◽  
Broadleaf Species

Abstract Changing climatic conditions are transforming the ecological and silvicultural roles of broadleaf tree species in northern Europe. Small-leaved lime (Tilia cordata Mill.) is distributed throughout most of Europe, and is a common broadleaf species in Latvia. This species can tolerate a broad range of environmental and ecological conditions, including temperature, water availability, and soil types. The aim of this study was to assess the genetic diversity and differentiation of Latvian T. cordata populations using nuclear microsatellite markers developed for Tilia platyphyllos. After testing of 15 microsatellite markers, Latvian T. cordata samples were genotyped at 14 micro-satellite loci. Latvian T. cordata populations had high genetic diversity, and were not overly isolated from each other, with moderate gene flow between populations. No highly differentiated populations were identified. Vegetative reproduction was identified in most analysed populations, and almost one-third of analysed individuals are of clonal origin. T. cordata has high timber production potential under the current climatic and growth conditions in Latvia, and therefore this species has potential for use in forestry, as well as playing a significant role in maintaining biodiversity and other ecosystem services.

Evaluation of genetic diversity and structure of Vietnamese goat populations using multi locus microsatellite markers

2017 ◽  
Vol 148 ◽  
pp. 43-50 ◽  
Author(s):  
Le Thi Thuy ◽  
Dinh Van Binh ◽  
Nguyen Trong Binh ◽  
Luu Quang Minh ◽  
Tran Thi Thu Thuy ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Genetic Diversity And Structure

scholarly journals Insight into the Current Genetic Diversity and Population Structure of Domestic Reindeer (Rangifer tarandus) in Russia

Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1309
Author(s):  
Veronika Kharzinova ◽  
Arsen Dotsev ◽  
Anastasiya Solovieva ◽  
Olga Sergeeva ◽  
Georgiy Bryzgalov ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Rangifer Tarandus ◽  
Allelic Richness ◽  
Environmental Changes ◽  
Principal Component ◽  
Genetic Structuring ◽  
Habitat Location ◽  
The Republic ◽  
Insight Into

To examine the genetic diversity and population structure of domestic reindeer, using the BovineHD BeadChip, we genotyped reindeer individuals belonging to the Nenets breed of the five main breeding regions, the Even breed of the Republic of Sakha, the Evenk breed of the Krasnoyarsk and Yakutia regions, and the Chukotka breed of the Chukotka region and its within-breed ecotype, namely, the Chukotka–Khargin, which is bred in Yakutia. The Chukotka reindeer was shown to have the lowest genetic diversity in terms of the allelic richness and heterozygosity indicators. The principal component analysis (PCA) results are consistent with the neighbor-net tree topology, dividing the reindeer into groups according to their habitat location and origin of the breed. Admixture analysis indicated a genetic structuring of two groups of Chukotka origin, the Even breed and most of the geographical groups of the Nenets breed, with the exception of the Murmansk reindeer, the gene pool of which was comprised of the Nenets and apparently the native Sami reindeer. The presence of a genetic component of the Nenets breed in some reindeer inhabiting the Krasnoyarsk region was detected. Our results provide a deeper insight into the current intra-breeding reindeer genetic diversity, which is an important requirement for future reindeer herding strategies and for animal adaptation to environmental changes.

scholarly journals Genetic diversity and phylogeography of Jeju Orthohantavirus (Hantaviridae) in the Republic of Korea

Virology ◽  
2020 ◽  
Vol 543 ◽  
pp. 13-19
Author(s):  
Seung-Ho Lee ◽  
Won-Keun Kim ◽  
Kyungmin Park ◽  
Jin Sun No ◽  
Geum-Young Lee ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Republic Of Korea ◽  
The Republic

Genetic diversity and structure of local apple cultivars from Northeastern Spain assessed by microsatellite markers

2012 ◽  
Vol 8 (6) ◽  
pp. 1163-1180 ◽  
Author(s):  
Jorge Urrestarazu ◽  
Carlos Miranda ◽  
Luis G. Santesteban ◽  
José B. Royo
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Genetic Diversity And Structure ◽  
Apple Cultivars

Recent distribution changes affect geographic clines in genetic diversity and structure of Pinus densiflora natural populations in Japan

2013 ◽  
Vol 304 ◽  
pp. 407-416 ◽  
Author(s):  
Masakazu G. Iwaizumi ◽  
Yoshiaki Tsuda ◽  
Masato Ohtani ◽  
Yoshihiko Tsumura ◽  
Makoto Takahashi
Keyword(s):  
Genetic Diversity ◽  
Pinus Densiflora ◽  
Natural Populations ◽  
Genetic Diversity And Structure
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