scholarly journals Deciphering S-RNase Allele Patterns in Cultivated and Wild Accessions of Italian Pear Germplasm

Forests ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1228
Author(s):  
Stefania Bennici ◽  
Mario Di Guardo ◽  
Gaetano Distefano ◽  
Giuseppina Las Casas ◽  
Filippo Ferlito ◽  
...  

The genus Pyrus is characterized by an S-RNase-based gametophytic self-incompatibility (GSI) system, a mechanism that promotes outbreeding and prevents self-fertilization. While the S-genotype of the most widely known pear cultivars was already described, little is known on the S-allele variability within local accessions. The study was conducted on 86 accessions encompassing most of the local Sicilian varieties selected for their traits of agronomic interest and complemented with some accessions of related wild species (P. pyrifolia Nakai, P. amygdaliformis Vill.) and some national and international cultivars used as references. The employment of consensus and specific primers enabled the detection of 24 S-alleles combined in 48 S-genotypes. Results shed light on the distribution of the S-alleles among accessions, with wild species and international cultivars characterized by a high diversity and local accessions showing a more heterogeneous distribution of the S-alleles, likely reflecting a more complex history of hybridization. The S-allele distribution was largely in agreement with the genetic structure of the studied collection. In particular, the “wild” genetic background was often characterized by the same S-alleles detected in P. pyrifolia and P. amygdaliformis. The analysis of the S-allele distribution provided novel insight into the contribution of the wild and international cultivars to the genetic background of the local Sicilian or national accessions. Furthermore, these results provide information that can be readily employed by breeders for the set-up of novel mating schemes.

2020 ◽  
Author(s):  
Alexander Harkness ◽  
Yaniv Brandvain

1SummaryTraditionally, we expect that self-incompatibility alleles (S-alleles), which prevent self-fertilization, should benefit from negative-frequency dependent selection and rise to high frequency when introduced to a new population through gene flow. However, the most taxonomically widespread form of self-incompatibility, the ribonuclease-based system ancestral to the core eudicots, functions through nonself-recognition, which drastically alters the process of S-allele diversification.We analyze a model of S-allele evolution in two populations connected by migration, focusing on comparisons among the fates of S-alleles originally unique to each population and those shared among populations.We find that both shared and unique S-alleles originating from the population with more unique S-alleles were usually fitter than S-alleles from the population with fewer. Resident S-alleles were often driven extinct and replaced by migrant S-alleles, though this outcome could be averted by pollen limitation or biased migration.Nonself-recognition-based self-incompatibility will usually either disfavor introgression of S-alleles or result in the whole-sale replacement of S-alleles from one population with those from another.


Author(s):  
Е. V. Bezlepkina ◽  
А. А. Gulyaeva ◽  
А. V. Pikunova

Self-incompatibility is one of the most important mechanisms used by flowering plants to prevent self-fertilization and, consequently, to provide the genetic diversity of population. The self-incompatibility in Prunus is controlled by two genes as minimum: S (self-incompatibility) and SFB (S haplotype-specific F-box protein).  S gene is represented in the population by a multiple allele series. Compatibility in pollination is dependent on the S allele combination of the cultivars. When S allele in the pollen is identical with one of the S alleles of the mother plant fertilization is arrested. Thus, both self-fertilization and fertilization by pollen of closely related plants (having identical S alleles) are prevented. This mechanism may be interrupted in the case of mutations in the S or SFB gene, which leads to the appearance of self-compatibility cultivars, and polyploidization, such as in sour cherry. The investigation of the S gene allele polymorphism of sweet cherry cultivars of VNIISPK breeding was performed as a part of the study of the gene collection of stone crops. Both consensus (PaConsI, PaConsII) and allele-specific (S1, S5, S9, S10) primers were used. The S-genotype of cultivars Adelina (S3/S5), Poezia (S3/S5), Siana (S3/S6), Orlovskaia feia (S3/S5) and Trosnianskaia (S5/S6) were established. The S-genotype of cultivars Malish, Podarok Orlu, Orlovskaia rozovaia and Orlovskaia yantarnaia was determined partially, as these cultivars have unique previously undescribed or very rare S alleles. Podarok Orlu variety has S9 allele and undescribed one. Malish and Orlovskaia yantarnaia varieties have S6 allele and S17 or S30 alleles supposedly, for these alleles specific primers have not yet been developed. Orlovskaya rozovaia has S6 allele and undescribed previously one.


HortScience ◽  
2004 ◽  
Vol 39 (5) ◽  
pp. 943-947 ◽  
Author(s):  
Wim Broothaerts ◽  
Ilse Van Nerum ◽  
Johan Keulemans

Apple cultivars display a self-incompatibility system that restricts self-fertilization and fertilization between cultivars bearing identical S-alleles. There has been considerable progress in identification of S-alleles in apple in recent years and methods are now available for the accurate S-genotyping of cultivars. Following a recently revised numerical identification system for apple S-alleles, we present the first extensive compilation of apple cultivars with their S-genotypes. This list contains data from our own investigations using S-allele-specific PCR methodology, including a number of new data, as well as published data from various other sources. Eighteen different S-alleles are discriminated, which allowed the determination of the S-genotypes for 150 diploid or triploid European, American, and Japanese cultivars. Many of these cultivars are cultivated worldwide for their fruit. Also included are a number of old, obsolete cultivars and a few nondomestic genotypes. We observed a wide variation in the frequency of S-alleles in the apple germplasm. Three S-alleles (S2, S3, and S9) are very common in the cultivars evaluated, presumably as a result of the widespread use of the same breeding parents, and seven alleles are very rare (S4, S6, S8, S16, S22, S23, S26).


2014 ◽  
Vol 4 (3) ◽  
pp. 95-98
Author(s):  
Iana Proskurkina

Abstract The growing number of foreign applicants looking forward to getting education in Ukrainian medical universities makes us find the ways how to improve and make effective the pre-professional training system of foreign medical applicants for further education. The article deals with the issues of the history of formation and development of the preprofessional training system of foreign medical applicants in Ukraine. On the ground of the electronic databases of the official websites of higher educational establishments, the data on years of opening first offices of the dean, departments and preparatory faculties for foreign medical applicants in Ukrainian medical universities are analyzed and systematized. Also the data on the setting up preparatory faculties at other universities who carry out licensed training of foreign students of the medical profile are presented. The data on the operating and management of such institutions in the system of the University administration are generalized. It’s revealed that during the years of its functioning the pre-professional training has changed, in particular the system was commercialized and the institutions involved in training foreign applicants have been reorganized. The modern trends in teaching foreign medical students at the preparatory faculties of the Ukrainian medical universities are displayed. Based on the analysis of the data it is concluded that the system of the pre-professional training of foreign medical applicants was set up in the 50s-60s years of the twentieth century. During this time, some positive experience in the preparation of future international medical specialists has been gained. The system of the pre-professional training of foreign medical applicants has been comprehensively improved and an effective system of managing foreign medical applicants has been created.


2019 ◽  
pp. 134-197
Author(s):  
V.E. . Sergei

The article is dedicated to the history of the Military Historical Museum of Artillery, Engineering and Signal Corps. The author examines the main stages of the museums formation, starting with the foundation of the Arsenal, established in St. Petersburg at the orders of Peter the Great on August 29th 1703 for the safekeeping and preservation of memory, for eternal glory of unique arms and military trophies. In 1756, on the base of the Arsenals collection, the General Inspector of Artillery Count P.I. created the Memorial Hall, set up at the Arsenal, on St. Petersburgs Liteyny Avenue. By the end of the 18th century the collection included over 6,000 exhibits. In 1868 the Memorial Hall was transferred to the New Arsenal, at the Crownwork of the Petropavlovsky Fortress, and renamed the Artillery Museum (since 1903 the Artillery Historical Museum). A large part of the credit for the development and popularization of the collection must be given to the historian N.E. Brandenburg, the man rightly considered the founder of Russias military museums, who was the chief curator from 1872 to 1903. During the Civil and Great Patriotic Wars a significant part of the museums holdings were evacuated to Yaroslavl and Novosibirsk. Thanks to the undying devotion of the museums staff, it not only survived, but increased its collection. In the 1960s over 100,000 exhibits were transferred from the holdings of the Central Historical Museum of Military Engineering and the Military Signal Corps Museum. In 1991 the collection also received the entire Museum of General Field Marshal M.I. Kutuzov, transferred from the Polish town of Bolesawjec. The Military Historical Museum of Artillery, Engineering and Signal Coprs is now one of the largest museums of military history in the world. It holds an invaluable collection of artillery and ammunition, of firearms and cold steel arms, military engineering and signal technology, military banners, uniforms, a rich collection of paintings and graphic works, orders and medals, as well as extensive archives, all dedicated to the history of Russian artillery and the feats of our nations defenders.Статья посвящена истории создания ВоенноИсторического музея артиллерии, инженерных войск и войск связи. Автор рассматривает основные этапы становления музея, начиная с основания Арсенала, созданного в СанктПетербурге по приказу Петра I 29 августа 1703 года для хранения и сохранения памяти, во имя вечной славы уникального оружия и военных трофеев. В 1756 году на базе коллекции Арсенала генеральный инспектор артиллерии граф П. И. создал мемориальный зал, установленный при Арсенале, на Литейном проспекте СанктПетербурга. К концу 18 века коллекция насчитывала более 6000 экспонатов. В 1868 году Мемориальный зал был перенесен в Новый Арсенал, на венец Петропавловской крепости, и переименован в Артиллерийский музей (с 1903 года Артиллерийский Исторический музей). Большая заслуга в развитии и популяризации коллекции принадлежит историку Н.Е. Бранденбургу, человеку, по праву считавшемуся основателем российских военных музеев, который был главным хранителем с 1872 по 1903 год. В годы Гражданской и Великой Отечественной войн значительная часть фондов музея была эвакуирована в Ярославль и Новосибирск. Благодаря неусыпной преданности сотрудников музея, он не только сохранился, но и пополнил свою коллекцию. В 1960х годах более 100 000 экспонатов были переданы из фондов Центрального исторического военноинженерного музея и Музея войск связи. В 1991 году коллекцию также получил весь музей генералфельдмаршала М. И. Кутузова, переданный из польского города Болеславец. Военноисторический музей артиллерии, инженерных войск и войск связи в настоящее время является одним из крупнейших музеев военной истории в мире. Здесь хранится бесценная коллекция артиллерии и боеприпасов, огнестрельного и холодного оружия, военной техники и сигнальной техники, военных знамен, обмундирования, богатая коллекция живописных и графических работ, орденов и медалей, а также обширные архивы, посвященные истории русской артиллерии и подвигам защитников нашего народа.


1971 ◽  
Vol 3 (4) ◽  
pp. 473-492 ◽  
Author(s):  
Laurie Taylor

Editorial note. March 17th, 1971 was the fiftieth anniversary of the opening by Marie Stopes of her birth control clinic in Holloway, London, the first of its kind in the UK and possibly in the world. In recognition of this notable event, the Board of the Marie Stopes Memorial Foundation, in conjunction with the University of York, has established a Marie Stopes Memorial Lecture to be given annually for a term of years. The first of the series was delivered on 12th March in the Department of Sociology, University of York, by Mr Laurie Taylor of that department. In introducing the speaker, Dr G. C. L. Bertram, the Chairman, emphasized the great contribution made by Marie Stopes to human welfare and gave a brief history of the clinic, which was soon moved to Whitfield Street. On Marie Stopes' death in 1958 the Memorial Foundation was set up to manage the clinic, still in Whitfield Street, and as a working monument to a great women.Mr Taylor's script is printed below as delivered and it will be seen that the lecture was a notable one. Not only that, but it was delivered with the verve of a Shakespearean actor and the members of the large and appreciative audience will not readily forget the occasion.


2005 ◽  
Vol 14 (1) ◽  
pp. 23-37 ◽  
Author(s):  
Louis Balthazar

This paper's objective is to bring forth some elements which confirm the following hypothesis : Canada is consigned to continentalism, namely to economic and cultural integration with the United States though this fact is shrouded in a Canadian nationalism of sorts. The continentalist mentality is rooted in the history of British North America, inhabited mostly by refugees from America who have remained inherently "Yankees" in spite of their anti-americanism. The Confederation itself is based on a sort of complicity with the United States. More recently there were talks of a "North American nationality", and continentalism both cultural and economic has come to be seen as a 'force of nature" which the governments, at the most, put into a chanelling process. Still, it is possible for Canadian nationalism to exist provided it does not go beyond the threshold whence it would run headlong into the continental mentality. Canada has defined itself through an international or non-national perspective far too long for today's nationalism not to remain weak and poorly established. But the Americans whose "manifest destiny" has succeeded in spreading over Canada without even their having tried to hoist their flag there find it to their advantage to maintain some form of Canadian sovereignty. Canada as a "friendly nation" can be of use to Washington. That is why there are almost as many advocates for Canada's independence in the United States as there are north of the border. Canadian nationalism can thus further the interests of some Canadian elites without seriously prejudicing continental integration which can very well afford not to be set up into formalized structures.


2010 ◽  
Vol 35 (3) ◽  
pp. 291-292
Author(s):  
B. ANANTHAKRISHNAN

Modern academic training for theatre in India has a history of just over fifty years (since independence). The National School of Drama (NSD) was set up in 1957, but the prime objective of the institution at that time was to generate professionals to develop children's theatre and rural theatre. Although India possessed a wide range of traditional performance cultures throughout the country, from rituals to folk performances and classical performances, the NSD was modelled on the Royal Academy of Dramatic Arts (RADA) since the new institution was led by a graduate of RADA, Professor Ebrahim Alkazi, who put the institution on a functional track. Thus the toolkit used during the initial days was primarily based on Western models conducive to realism rather than growing organically out of the actual practices of the different forms of Indian performance. This early orientation remains today, emphasizing the creation of referential meanings on the stage through conventional methods and devices, taken as the unshakable organizing principle of theatre practice.


Materials ◽  
2021 ◽  
Vol 14 (8) ◽  
pp. 2077
Author(s):  
Oliver Zeman ◽  
Michael Schwenn ◽  
Martin Granig ◽  
Konrad Bergmeister

The assessment of already installed anchorages for a possible exceeding of the service load level is a question that is gaining more and more importance, especially in building maintenance. Bonded anchors are of particular interest here, as the detection of a capacity reduction or load exceedance can cause damage to the concrete-bonded mortar behavior. This article investigates the extent to which ultrasonic methods can be used to make a prediction about the condition of anchorages in concrete and about their load history. A promising innovative assessment method has been developed. The challenges in carrying out the experimental investigations are the arrangement of the transducers, the design of the test set-up and the applicability of direct, indirect or semidirect ultrasonic transmission. The experimental investigations carried out on a test concrete mix and a bonded anchor system show that damage to the concrete structure can be detected by means of ultrasound. The results indicate the formation of cracks and therefore a weakening of the response determined by means of direct, indirect and semidirect ultrasonic transmission. However, for application under non-laboratory conditions and on anchors with unknown load history, the calibration with a reference anchor and the identification of the maximum load is required. This enables a referencing of the other loaded anchors to the unloaded conditions and allows an estimation of the load history of individual anchors.


Author(s):  
Efim I. Pivovar ◽  

The article covers the activities of the Association called Business Centre for Economic Development of the Commonwealth of Independent States. The author examines in detail the twenty-year history of this organisation, its creation, goals and objectives, features of the structure, participants and partners, and the formation of competence areas. The article emphasises that over the years of its existence, the Association has become an important and effective platform for interaction among business structures, industry associations, banking and financial institutions, entrepreneurs, the expert and scientific community as well as the media of the Commonwealth countries. Considering the Association’s key areas and forms of activity the author highlights the annual International Economic Forums involving business leaders of the Commonwealth member states, which contribute to the strengthening and development of multilateral economic relations in the post-Soviet space. The article examines in great detail the work of each Forum, its participants (both individuals and legal entities), the topics of discussions and speeches of individual speakers from various Commonwealth countries, which, in the author’s opinion, are of crucial importance for the activities of the Association called Business Centre for Economic Development of the Commonwealth of Independent States and the entire Commonwealth, as well as for the development of Eurasian integration. In addition, the article focuses on the final documents of the Forums: the author studies their main provisions with the set up in them goals, tasks, proposals and priorities.


Sign in / Sign up

Export Citation Format

Share Document