scholarly journals Pollen Morphology and Variability of Abies alba Mill. Genotypes from South-Western Poland

Forests ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1125
Author(s):  
Dorota Wrońska-Pilarek ◽  
Monika Dering ◽  
Jan Bocianowski ◽  
Kacper Lechowicz ◽  
Wojciech Kowalkowski ◽  
...  
Keyword(s):  
Pollen Morphology ◽  
Geographical Location ◽  
Abies Alba ◽  
Intraspecific Variability ◽  
Pollen Grains ◽  
Polar Axis ◽  
Seed Orchard ◽  
Diagnostic Features ◽  
Pollen Shape ◽  
First Time

The objective of this study was to investigate pollen morphology and ranges of intraspecific variability of Abies alba Mill. Pollen grains were collected from nine clonal seed orchards of A. alba in the Sudety Mountains, (South-Western Poland). At each seed orchard, 4–6 grafts were selected. Each individual (graft) was represented by 30 pollen grains and 1440 pollen grains were measured totally. Eight quantitative and four qualitative features of pollen grains were analysed. The diagnostic features of pollen grains for the studied species were: Exine surface of pollen corpus (cappa and leptoma) and sacci, the length of the polar axis (P), pollen shape (P/E ratio), and a new trait—saccus shape (A/B ratio — saccus width (A) to his length (B)). Pollen features made possible to differentiate seven individual genotypes (samples). To our knowledge, this is the first time that the intraspecific and interindividual variability of pollen grains of A. alba were investigated. The most different were the pollen grains from samples—genotypes 13 (Bystrzyca Kłodzka) and 18 (Jugów), and also (although to a lesser extent) genotypes—11 (Kamienna Góra), 30, 31 (Jugów), and 44 (Szklarska Poręba). No significant relationships were observed between the pollen grain traits and the geographical location of the collection sites.

scholarly journals Morphology of pollen in Ferula genus (Apiaceae)

PhytoKeys ◽  
2021 ◽  
Vol 179 ◽  
pp. 111-128
Author(s):  
Birol Başer ◽  
Mehmet Sagıroglu ◽  
Gulden Dogan ◽  
Hayri Duman
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Morphological Characteristics ◽  
Pollen Grains ◽  
Polar Axis ◽  
Average Linkage ◽  
Pollen Shape ◽  
First Time ◽  
Scanning Electron

In this study, the pollen morphology of all Ferula species distributed throughout the country of Turkey was studied with light and scanning electron microscopy for the first time. The aim is to identify the pollen morphological characteristics of 23 Ferula species. The pollen is radially symmetrical, isopolar and tricolporate in all examined species. Pollen grains are prolate and perprolate with the polar axis ranging from 22.28 to 40.47 µm and the equatorial axis from 13.70 to 18.73 µm. Their polar shapes are triangular, triangular to subtriangular and circular to subcircular. Several types of exine ornamentations have been observed on pollen through the use of scanning electron microscopy. The dendrogram constructed by using Average Linkage of the examined data revealed two main groups. It was determined that some pollen characteristics are more useful for classification than others. In particular, P, E, the ratio of P/E (pollen shape) and ornamentation in the polar and equatorial views are the most valuable variables for discrimination the Ferula species.

scholarly journals Pollen morphology of the Balkan-Carpathian endemic Campanula lingulata Waldst. & Kit. (Campanulaceae)

2016 ◽  
pp. 75-82
Author(s):  
Marina Macukanovic-Jocic ◽  
Snezana Jaric
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Endemic Species ◽  
Pollen Morphology ◽  
Average Length ◽  
Pollen Grains ◽  
Polar Axis ◽  
First Time ◽  
Scanning Electron

Palynomorphological characteristics of Campanula lingulata, the Balkan-Carpathian endemic species growing in Serbia, have been investigated using light microscopy and scanning electron microscopy for the first time, in order to provide some information helpful for a better understanding of the taxonomic position of this species within the genus, as well as to contribute to the pollen atlas of Serbian apiflora. The pollen grains are radially symmetrical, isopolar, 3-zonoporate and medium-sized monads oblate-sphaeroidal in shape. Mean of the polar axis (P) is 27.6?1.9 ?m, while the average length of the equatorial axis (E) is 28.8?1.6 ?m. The apertures are operculate. The sculpturing pattern of the exine is microre?ticulate-microechinatae. The exine surface is covered with evenly distributed supratectal spinules of variable length and sparse granules. The longest supratectal spinules are 0.64?0.05 ?m in length and the smallest sculptural elements are less than 0.2 ?m high. The microechinae density per sample area of 5 ?m x 5 ?m averages 17.4?2.4.

scholarly journals Pollen morphology of Malvaceae genera from Saudi Arabia and its taxonomic significance

2021 ◽  
pp. 725-742
Author(s):  
Kadry Abdel khalik ◽  
Suad Al- Ruzayza ◽  
Abdullah Assiri ◽  
Ahmed Elkordy
Keyword(s):  
Saudi Arabia ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar Axis ◽  
Morphological Characters ◽  
Average Height ◽  
Prolate Spheroidal ◽  
Oblate Spheroidal ◽  
Equatorial Diameter ◽  
Pollen Shape

Pollen morphology of 20 species belong to seven genera (Abutilon, Althaea, Hibiscus, Malva, Pavonia, Senra and Sida) of Malvaceae from Saudi Arabia were studied by using light microscope (LM) and scanning electron microscope (SEM). Quantitative and qualitative pollen morphological characters which vary among investigated taxa are found in the pollen polarity, symmetry, size, shape, polar axis, equatorial diameter, P/E ratio, average height and width of spine, aperature character and spine index. The pollen grains vary from spheroidal, prolate spheroidal, oblate spheroidal to suboblate. All taxa were characterized by relatively large to medium sized pollen grains, numerous pores scattered irregularly all over the grain, and echinate sculpturing. Sida ovata is the largest size pollen grain (138.95) µm. On the other hand, Malva parviflora showed the smallest pollen size (52.28 µm). The average height and width of spine varied greatly among studied taxa. The highest spines (20.65µm) found in Sida ovata, while the shortest (3.19 µm) was found in Abutilon pannosum. Results of the pollen shape, size, and exine sculpture characters offered useful data for evaluating the taxonomy of Malvaceae both on subgeneric and sectional levels. A key for the identification of the investigated taxa based on pollen grains characters is also provided

scholarly journals Pollen morphology of section Cheirolepis Boiss. of the genus Centaurea L. (Asteraceae) in Turkey

2019 ◽  
Vol 26 (2) ◽  
pp. 131-148
Author(s):  
Burcu Yilmaz Çitak ◽  
Hüseyin Dural ◽  
Tuna Uysal ◽  
Nur Münevver Pinar
Keyword(s):  
Electron Microscope ◽  
Clustering Analysis ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Base Length ◽  
Diagnostic Features ◽  
Spine Length ◽  
Pollen Characters ◽  
Pollen Shape ◽  
Scanning Electron

In this paper, the palynomorphology of 17 taxa of section Cheirolepis in Turkey, were investigated by light (LM) and scanning electron microscope (SEM). Detailed descriptions of the pollen grains were given for each taxon and a well-resolved dendrogram was generated through numerical analysis of palynological diagnostic features. The pollen grains were found to be radially symmetric, isopolar, and generally 3-zonocolporate, with the exception of C. derderiifolia, C. kotschyi var. floccosa, and C. saligna, which were also 4-zonocolporate. The shape of the pollen grains were prolatespheroidal, with the polar axes of 32.76–46.26 μm and equatorial axes of 31.86–45.82 μm. The sculpturing of the pollen grains was generally scabrate-perforate or rarely microechinate-perforate. The spines were conical with a changing base length. The length and the width of the spines varied between 0.48 and 2.28 μm and 0.4 and 3.39 μm, respectively. The number of perforations at the base of the spines ranged in two or morerows and they varied between 5 and 30. The number of spines was 16–70 in 10 μm2. The results of this study showed that the polar axes, equatorial axes, aperture type, pollen shape, spine length, perforation number, and number of spines in 10 μm2 are essential for distinguishing the studied taxa. The taxa were grouped by clustering analysis of selected pollen characters using the UPGMA method.

Pollen morphology of some Verbascum (Scrophulariaceae) taxa in Turkey

Phytotaxa ◽  
2018 ◽  
Vol 333 (2) ◽  
pp. 209 ◽  
Author(s):  
AYŞE ÖZTÜRK ◽  
KUDRET BETÜL GÜNEY ◽  
BARIŞ BANİ ◽  
KERİM GÜNEY ◽  
FAİK AHMET KARAVELİOĞULLARI ◽  
...  
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar Axis ◽  
Prolate Spheroidal ◽  
Oblate Spheroidal ◽  
General Terms ◽  
Surface Ornamentation ◽  
Pollen Shape ◽  
Axis Length ◽  
Scanning Electron

Pollen morphology of six Verbascum (Scrophulariaceae) taxa belonging to five different groups in the genus [V. georgicum (group E), V. nudatum var. nudatum (group F), V. cariense (group F), V. bithynicum (group H), V. pycnostachyum (group K), and V. lasianthum (group L)] was studied. Pollen slides were prepared according to the Woodhouse method and were examined with light-microscopy (LM) and Scanning Electron Microscopy (SEM). All studied pollen grains are generally monads, though tetrads (5 %) were observed in Verbascum nudatum var. nudatum. Pollen grains of all studied taxa were determined as isopolar and radially symmetric. In general terms, aperture types are trizonocolporate, but also trisyncolpate (V. lasianthum 10 %, V. pycnostachum 1 %, and V. georgicum 4 %) and trizonocolpate (V. cariense 10 %, V. bithynicum 10%, V. lasianthum 90%, V. pycnostachyum 9%, and V. georgicum 96 %) aperture types were observed. The shapes of the pollen grains were determined as prolate, subprolate, prolate-spheroidal and oblate-spheroidal. The sculpture was reticulate in all cases. The results of the study corroborate that morphological features, such as pollen shape, polar axis length (P), equatorial axis length (L), aperture type, surface ornamentation, muri thickness, and lumina shape, show differences which can be taken into consideration in the systematic discrimination within the groups, and also for discrimination of the groups in the genus.

scholarly journals Pollen morphology of Quercus (subgenus Quercus, section Quercus) in Iran and its systematic implication

2012 ◽  
Vol 81 (1) ◽  
pp. 33-41 ◽  
Author(s):  
Parisa Panahi ◽  
Mohammad R. Pourmajidian ◽  
Asghar Fallah ◽  
Mehdi Pourhashemi
Keyword(s):  
Pollen Morphology ◽  
Morphological Characteristics ◽  
Pollen Grains ◽  
Structural Pattern ◽  
Pollen Characters ◽  
The Family ◽  
Pollen Shape ◽  
First Time ◽  
The Relationship ◽  
Systematic Implication

For the first time, pollen morphology of 9 (4 spp. and 5 subspp.) taxa representing lobed leaved oaks of Iran in the family of Fagaceae has been examined and illustrated using light microscopy and scanning electron microscopy of acetolysed material. Detailed pollen morphological characteristics are given for <em>Quercus</em> section. The pollen grains are single, isopolar, radially symmetrical, tricolpate, tricolporoidate or tricolporate. Pollens were studied to show all possible characteristics like shape, size, apertures, wall thickness, etc., with special reference to the specific features of each pollen type such as structural, sculptural and suprasculptural patterns. There is considerable variation in pollen morphology between taxa so that, three types of pollen shape, five types of structural pattern, two types of sculptural pattern, five types of suprasculptural pattern and three types of perfora distribution are defined. Furthermore, the relationship between pollen morphology and taxonomy is discussed. Overall, pollen characters are shown to be a useful and informative tool for assessing taxonomic position within <em>Quercus</em> section in Iran.

scholarly journals Pollen morphology of Rosa gallica L. (Rosaceae) from Southern Poland

2011 ◽  
Vol 74 (4) ◽  
pp. 297-304 ◽  
Author(s):  
Dorota Wrońska-Pilarek ◽  
Krystyna Boratyńska
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar Axis ◽  
Pollen Grain ◽  
The Other ◽  
Diagnostic Features ◽  
Linear Arrangement ◽  
Pleistocene Refugia ◽  
Southern Poland ◽  
Rosa Gallica

The morphology of pollen grains of <em>Rosa gallica</em> was studied on the basis of material from 15 natural localities in southern Poland. It was ascertained that the diagnostic features of pollen grains of <em>R. gallica</em> were: the presence of deep, often Y-shaped striae in a linear arrangement; numerous perforations of various, rather big diameters on the bottom of striae; the occurrence of operculum on the membrane of ectocolpus; the presence of costae ectocolpi and fastigium in the area of endopori. The most significant differences among the particular samples studied concern the shape of grains. The differences between pollen grains of 15 individuals of <em>Rosa gallica</em> from southern Poland are generally slight. The most discriminating measured character is length of equatorial axis of pollen grain and length of ectocolpi and thickness of exine along polar axis. The remaining differentiate the individuals with smaller significance. The differences between Silesian samples and all the other examined ones may result from origin of different Pleistocene refugia.

scholarly journals Pollen Morphology of Some Turkish Salvia L. (Lamiaceae: Mentheae) Species

2020 ◽  
Vol 38 ◽  
Author(s):  
K. AKTA ◽  
C. ÖZDEMIR ◽  
M. ÖZKAN ◽  
P. BARAN
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar Axis ◽  
Radial Symmetry ◽  
Exine Ornamentation ◽  
Pollen Shape ◽  
Axis Length ◽  
Different Characteristics ◽  
Scanning Electron

ABSTRACT: In this presented work, the pollen morphologies of twenty-one taxa, nine of them endemic from Turkey, belonging to the genus Salvia (Lamiaceae: sub-family Nepetoideae: tribe Mentheae: sub-tribe Salviinae), S. aethiopis L., S. argentea L., S. aytachii Vural & Adigüzel, S. blepharochlaena Hedge & Hub.-Mor., S. cadmica Boiss., S. ceratophylla L., S. cryptantha Montbret & Aucher, S. frigida Boiss., S. fructicosa Miller, S. halophile Hedge, S. napifolia Jacq., S. microstegia Boiss. & Bal., S. recognita Fisch. & Mey. S. sclarea L., S. smyrnaea Boiss. S. suffruticosa Montbret & Aucher, S. tchihatcheffii (Fisch. & Mey.) Boiss., S. tometosa Bertol, S. verbenaca L., S. viridis L., and S. wiedemanni Boiss. and collected throughout the Turkey, have been intensively studied by using light (LM) and a scanning electron (SEM) microscopy. The objectives of this study are to investigate the pollen morphologies of twenty-one taxa of Turkish Salvia (nine of which are endemic) and to present similar and different characteristics of the taxa. The investigated whole pollen grains had a structure of both isopolar and radial symmetry. Overall, types of aperture were mostly hexacolpate, but it was also determined that the aperture type of S. viridis was both hexacolpate and octacolpate. The pollen grains had characteristic shapes of spheroidal, suboblate, prolate and subprolate. The exine ornamentation was bireticulate and reticulate-perforate. It was determined by the results of palynological studies that pollen morphology changed amongst these studied taxa. As a conclusion, the results of the present study show that palynological characters such as pollen shape, polar axis length (P), equatorial axis length (E), aperture numbers and types and exine ornamentation, exhibit remarkable differences amongst the studied taxa.

scholarly journals Pollen morphology of four endemic species of Pedicularis L. from alpine zone of the Deosai Plateau, Himalayan range

2012 ◽  
Vol 19 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Abida Bano ◽  
Mushtaq Ahmad ◽  
Mir Ajab Khan ◽  
Muhammad Zafar ◽  
Shazia Sultana ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Alpine Zone ◽  
Exine Sculpturing ◽  
Plant Taxon ◽  
Pollen Shape ◽  
First Time ◽  
Fertility Estimation ◽  
Scanning Electron

The pollen morphology with special reference to exine sculpturing of four species of the genus Pedicularis L. has been examined by light and scanning electron microscope. Comparative pollen analysis was made based on the type of pollen, shape in polar and equatorial views, P/E ratio, exine thickness and sculpturing of pollen. In this study, two types of pollen aperture configuration known in the genus were observed i.e., trisyncolpate and bi-syncolpate. Pollen grains with microscabrate ornamentation were found in bi-syncolpate pollen for the first time. Pollen fertility estimation ranged from 87-95%, which shows that pollen flora of selected species is well established in Alpine zone.DOI: http://dx.doi.org/10.3329/bjpt.v19i1.10935Bangladesh J. Plant Taxon. 19(1): 1-5, 2012 (June)

scholarly journals Pollen morphology of Polish species from the genus Rubus L. (Rosaceae) and its systematic importance

2019 ◽  
Author(s):  
Kacper Lechowicz ◽  
Dorota Wrońska-Pilarek ◽  
Jan Bocianowski ◽  
Tomasz Maliński
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar Axis ◽  
The Other ◽  
Individual Species ◽  
Bridge Structure ◽  
Interspecific Variability ◽  
Exine Ornamentation ◽  
First Time ◽  
Systematic Importance

AbstractThe genus Rubus L. (Rosaceae) has as yet not been investigated satisfactorily in terms of palynology. This genus is taxonomically very difficult due to the large number of species and problems with their delimitation, as well as very different distribution areas of particular species. The aim of this study was to investigate pollen morphology and for the first time the ranges of intrageneric and interspecific variability of Rubus species, as well as verify the taxonomic usefulness of these traits in distinguishing studied taxa from this genus. They were analysed for 11 quantitative pollen characteristics and the following qualitative ones: exine ornamentation, pollen outline and shape, as well as bridge structure. Analyses were conducted on a total of 1740 pollen grains, which represent 58 blackberry species belonging to a majority of subgenera and all the sections and series found in Poland. The diagnostic characters included exine ornamentation (exine ornamentation type, width and direction of grooves and striae, number and diameter of perforations) and length of the polar axis (P). The arrangement of the examined species on the dendrogram does not corroborate division of the genus Rubus into subgenera, sections and series currently adopted in taxonomy. The lack of dependence may result from apomixis observed in Rubus, which could reduce natural variability. Pollen features should be treated in taxonomy as auxiliary, because they fail to differentiate several (10) individual species, while the other ones create groups with similar pollen traits.

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