scholarly journals Molecular Data Reveal Unrecognized Diversity in the European Ganoderma resinaceum

Forests ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 850
Author(s):  
Kateřina Náplavová ◽  
Terézia Beck ◽  
Peter Pristaš ◽  
Svetlana Gáperová ◽  
Martin Šebesta ◽  
...  
Keyword(s):  
Sequence Data ◽  
Large Subunit ◽  
Elongation Factor ◽  
Molecular Data ◽  
Urban Trees ◽  
Translation Elongation ◽  
Ionization Time ◽  
Basidiomycetous Fungus ◽  
Flight Mass Spectrometry ◽  
First Time

Ganoderma resinaceum Boud. is commonly found in Mediterranean region, but rarely in Western, Central or Eastern Europe. It is a parasitic basidiomycetous fungus causing stem decay—especially in urban trees. A collection of nine fungal specimens from Slovakia (Central Europe), morphologically identified as G. resinaceum, was recently studied on the basis of sequence data from the internal transcribed spacer (ITS) regions. Analyses showed that the collections clustered into two separate groups. In this study—for the first time—the sequences of other molecular markers, namely partial translation elongation factor (tef1-α) region and partial 25S large subunit ribosomal RNA gene (25S LSU rRNA), as well as matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI–TOF MS) were obtained and used to evaluate the genetic variability of G. resinaceum. All these analyses confirm the existence of two previously unrecognized genotypes within the morphospecies.

Affinities of the Boletus chromapes group to Royoungia and the description of two new genera, Harrya and Australopilus

2012 ◽  
Vol 25 (6) ◽  
pp. 418 ◽  
Author(s):  
Roy E. Halling ◽  
Mitchell Nuhn ◽  
Todd Osmundson ◽  
Nigel Fechner ◽  
James M. Trappe ◽  
...  
Keyword(s):  
New Genus ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Sister Group ◽  
Molecular Data ◽  
Translation Elongation ◽  
Spore Deposit ◽  
Large Subunit Rdna ◽  
Translation Elongation Factor 1Α

Harrya is described as a new genus of Boletaceae to accommodate Boletus chromapes, a pink-capped bolete with a finely scabrous stipe adorned with pink scabers, a chrome yellow base and a reddish-brown spore deposit. Phylogenetic analyses of large-subunit rDNA and translation elongation factor 1α confirmed Harrya as a unique generic lineage with two species, one of which is newly described (H. atriceps). Some Chinese taxa were recently placed in a separate genus, Zangia, supported by both morphology and molecular data. Multiple accessions from Queensland, Australia, support the synonymy of at least three species in a separate Australian clade in the new genus, Australopilus. The truffle-like Royoungia is also supported as a separate lineage in this clade of boletes. Even though it lacks stipe characters, it possesses the deep, bright yellow to orange pigments in the peridium. Additional collections from Zambia and Thailand represent independent lineages of uncertain phylogenetic placement in the Chromapes complex, but sampling is insufficient for formal description of new species. Specimens from Java referable to Tylopilus pernanus appear to be a sister group of the Harrya lineage.

Morphological characters and phylogenetic analysis reveal a new species of Phellinus with hooked hymenial setae from Vietnam

Phytotaxa ◽  
2018 ◽  
Vol 356 (1) ◽  
pp. 91 ◽  
Author(s):  
LIN ZHU ◽  
XING JI ◽  
JING SI ◽  
BAO-KAI CUI
Keyword(s):  
Rna Polymerase Ii ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characters ◽  
Molecular Data ◽  
Translation Elongation ◽  
The Status ◽  
Large Subunit Rdna ◽  
Elongation Factor 1 ◽  
A New Species

Phellinus vietnamensis sp. nov. is described from Vietnam based on morphological characters and molecular data. Morphologically, it is characterized by perennial, pileate basidiomata, a dimitic hyphal system, hooked hymenial setae, and colorless, broadly subglobose to ovoid, thick-walled basidiospores 5.5–6 × 4.8–5.2 μm. Phylogenetically, the status of Phellinus vietnamensis is strongly supported based on sequences of the nuclear internal transcribed spacer (ITS) regions, the translation elongation factor 1-α gene (EF1-α) nuclear large subunit rDNA (nrLSU) and the second largest subunits of RNA polymerase II (RPB2).

A new species and a new combination in the genus Aureoboletus (Boletales, Boletaceae) from southern China

Phytotaxa ◽  
2015 ◽  
Vol 222 (2) ◽  
pp. 129 ◽  
Author(s):  
Nian-Kai Zeng ◽  
MING ZHANG ◽  
ZHI-QUN Liang
Keyword(s):  
Rna Polymerase Ii ◽  
Southern China ◽  
Large Subunit ◽  
Line Drawing ◽  
Elongation Factor ◽  
Molecular Data ◽  
New Combination ◽  
Translation Elongation ◽  
Microscopic Features ◽  
A New Species

Two lineages of Aureoboletus (Boletales, Boletaceae) from southern China were revealed by using molecular data based on combined dataset of the nuclear ribosomal large subunit RNA (nrLSU), the translation elongation factor apha-1 (tef1-a) and the largest subunit of RNA polymerase II (rpb1). One of them corresponds with the previous morphology-based taxon, viz. Boletellus longicollis, another one is different from those taxa described based on morphological features. And, thus, Auroboletus clavatus sp. nov. and A. longicollis comb. nov. were proposed. A detailed description, colour photos of fresh basidiomata, and a line-drawing of microscopic features of the two taxa were provided.

Alanomyces, a new genus of Aplosporellaceae based on four loci phylogeny

Phytotaxa ◽  
2017 ◽  
Vol 297 (2) ◽  
pp. 168 ◽  
Author(s):  
ROHIT SHARMA ◽  
GIRISH KULKARNI ◽  
MAHESH S. SONAWANE
Keyword(s):  
Sequence Data ◽  
Large Subunit ◽  
Elongation Factor ◽  
Fruit Trees ◽  
Fruit Rot ◽  
Close Relative ◽  
Natural Forests ◽  
Translation Elongation ◽  
Dna Sequence Data ◽  
Large Subunit Rdna

The Botryosphaeriales is a cosmopolitan fungal order and genera belonging to it are common opportunistic pathogens which mostly infect woody plants. They cause fruit rot, dieback, trunk rot, canker and similar diseases killing trees of natural forests, plantations and fruit trees. Relatively recently, some new families (including family Aplosporellaceae) had been established within this order delineating from family Botryosphaeriaceae. In the present study, two strains (MMI00067 and MMI00068) were isolated from soil attached to the base of a macrofungal stipe collected from the forest of Anuppur (Amarkantak), Madhya Pradesh, India. Strains were identified by DNA sequence data of four loci viz., internal transcribed spacer (ITS) of rDNA, large subunit rDNA (LSU), translation elongation factor (tef) and partial β-tubulin (βtub). Based on the phylogenetic analysis, a new fungal genus Alanomyces is proposed and is positioned within Aplosporellaceae along with Aplosporella. It is characterized by fast growing, dark greenish-black colony, long neck-like multilocular-papillate, black pycnidia with separate ostiole and small <10 µm in length, cylindrical, hyaline, guttulate spermatia. The genus Alanomyces is phylogenetically distinct from its close relative Aplosporella and proposed herein as a new monotypic genus with A. indica as type species.

New species of Nectria and Thyronectria from Xinjiang, China

Phytotaxa ◽  
2020 ◽  
Vol 433 (4) ◽  
pp. 253-264
Author(s):  
RONG MA ◽  
SHENG-NAN LI ◽  
YING ZHAO ◽  
MIN WANG ◽  
THEMIS J. MICHAILIDES ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Alpha Actin ◽  
First Time ◽  
Elongation Factor 1

Nectria berberidis sp. nov. and Thyronectria berberidicola sp. nov. isolated from Berberis heteropoda in Xinjiang Uygur Autonomous Region, China, are described and illustrated. Nectria berberidis is characterized by clavate asci (50–87 × 8–12 μm) with ellipsoidal to fusiform, 1-septate ascospores. Thyronectria berberidicola is characterized by clavate asci (117–25.9 × 63.7–117.9 μm) with ellipsoidal to fusiform ascospores that have 5–8 transverse septa and 1(–2) longitudinal septum. Ascospores bud to produce hyaline, bacillar ascoconidia. Phylogenetic analyses based on alpha-actin (ACT), the internal transcribed spacer (ITS), the large nuclear ribosomal RNA subunit (LSU), translation elongation factor 1-alpha (TEF1) and the β-tubulin (TUB) sequence data revealed that isolates of N. berberidis and T. berberidicola form a distinct clade within Nectria and Thyronectria, respectively. In addition, Nectria nigrescens is reported for the first time in China.

scholarly journals Re-collection of Dermea prunus in China, with a description of D. chinensis sp. nov.

MycoKeys ◽  
2019 ◽  
Vol 50 ◽  
pp. 79-91
Author(s):  
Ning Jiang ◽  
Cheng-Ming Tian
Keyword(s):  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Generic Concept ◽  
Frequent Use ◽  
Morphological And Molecular Data ◽  
First Time ◽  
Asexual Morphs

Dermea was protected against its synonym, Foveostroma, due to its well-circumscribed generic concept and more frequent use. We describe and illustrate Dermeachinensissp. nov. based on its morphological characteristics and a molecular analysis of the internal transcribed spacer (ITS) and large subunit (LSU) sequence data. Dermeachinensis is isolated from Betulaalbosinensis with sexual and asexual morphs and can be distinguished from D.molliuscula on Betula trees by its aseptate and wider ascospores. The connection between the two morphs is proved based on sequence data. Here, we describe the asexual morph of D.pruni for the first time based on morphological and molecular data from the same host and country of origin, and compare it with other species of Prunus.

scholarly journals New species of Retiboletus (Boletales, Boletaceae) from China based on morphological and molecular data

MycoKeys ◽  
2020 ◽  
Vol 67 ◽  
pp. 33-44
Author(s):  
Hai-Ying Liu ◽  
Yan-Chun Li ◽  
Tolgor Bau
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Large Subunit ◽  
Elongation Factor ◽  
Molecular Data ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Line Drawings ◽  
Morphological And Molecular Data ◽  
Elongation Factor 1

Species of the genus Retiboletus in China were investigated based on morphology and phylogenetic analysis of DNA sequences from the nuclear ribosomal large subunit (nrLSU) and the translation elongation factor 1-α gene (TEF1-α). Nine species were recovered from China, including two new and seven known species. The new species, namely Retiboletus ater and R. sinogriseus, from southwestern and northeastern China respectively, are documented and illustrated in this paper. Retiboletus ater is morphologically characterized by its black to grayish black pileus, white to grayish hymenophore, black to blackish stipe and white to grayish white context. Retiboletus sinogriseus is morphologically characterized by its brown to grayish-brown pileus, yellow to grayish-yellow hymenophore, pale yellow to brownish stipe and yellow to brownish-yellow context. Descriptions and line drawings of these two novel species and their comparisons with allied taxa are presented.

scholarly journals One New Species and Two New Host Records of Apiospora from Bamboo and Maize in Northern Thailand with Thirteen New Combinations

Life ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1071
Author(s):  
Xingguo Tian ◽  
Samantha C. Karunarathna ◽  
Ausana Mapook ◽  
Itthayakorn Promputtha ◽  
Jianchu Xu ◽  
...  
Keyword(s):  
Sequence Data ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
New Combinations ◽  
New Host ◽  
Translation Elongation ◽  
Northern Thailand ◽  
Dna Sequence Data ◽  
New Host Records

The genus Apiospora is known as a cosmopolitan genus, found across various substrates. In this study, four Apiospora taxa were obtained from the decaying stems of bamboo and maize in northern Thailand. Apiospora collections were compared with known species based on the morphological characteristics and the DNA sequence data of internal transcribed spacer (ITS), the partial large subunit nuclear rDNA (LSU), the translation elongation factor 1-alpha gene (TEF1-α) and beta-tubulins (TUB2). Apiospora chiangraiense sp. nov. and two new host records (Ap. intestini and Ap. rasikravindra) are introduced here based on the morphological characteristics and multi-locus analyses. Additionally, thirteen species previously identified as Arthrinium are introduced as new combinations in Apiospora, viz., Ap. acutiapica, Ap. bambusicola, Ap. biserialis, Ap. cordylines, Ap. cyclobalanopsidis, Ap. euphorbiae, Ap. gelatinosa, Ap. locuta-pollinis, Ap. minutispora, Ap. pseudorasikravindrae, Ap. septate, Ap. setariae and Ap. sorghi.

scholarly journals Multigene Phylogeny and Morphology Reveal Three Novel Species and a Novel Record of Agaricus From Northern Thailand

2021 ◽  
Vol 12 ◽  
Author(s):  
Chanyawat Jaichaliaw ◽  
Jaturong Kumla ◽  
Santhiti Vadthanarat ◽  
Nakarin Suwannarach ◽  
Saisamorn Lumyong
Keyword(s):  
Phylogenetic Trees ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Translation Elongation ◽  
Northern Thailand ◽  
Three New Species ◽  
First Time

Agaricus is a saprophytic mushroom genus widely distributed throughout the world. In this study, a survey of the Agaricus species carried out around Chiang Mai University in northern Thailand from 2018 to 2019 yielded 12 collections. Morphological characteristics and phylogenic analyses based on the internal transcribed spacers (ITS) and a fragment of the large subunit (LSU) of the nuclear ribosomal DNA (rDNA), and a fragment of the translation elongation factor 1-alpha (tef1) genes were investigated. The results revealed that these collections belong to six species including Agaricus erectosquamosus, Agaricus pallidobrunneus, Agaricus subrufescens, and three new species. Agaricus thailandensis sp. nov. was found to belong to Agaricus sect. Minores, which is placed in Agaricus subg. Minores. Aagricus pseudoerectosquamosus sp. nov. was placed in Agaricus sect. Brunneopicti within Agaricus subg. Pseudochitonia. Furthermore, Agaricus lannaensis remains an incertae sedis in Agaricus subg. Pseudochitonia. Additionally, this study was proposed that A. pallidobrunneus was discovered in Thailand for the first time. Full descriptions, color photographs, illustrations, and phylogenetic trees are provided.

scholarly journals Debunking Korunomyces

2021 ◽  
Author(s):  
Bruno W. Ferreira ◽  
Janaina L. Alves ◽  
Pedro W. Crous ◽  
Robert Barreto
Keyword(s):  
Dna Sequences ◽  
Large Subunit ◽  
Elongation Factor ◽  
Its Region ◽  
New Combination ◽  
Lsu Rdna ◽  
Translation Elongation ◽  
Miconia Calvescens ◽  
Pure Cultures ◽  
First Time

Abstract Korunomyces is a genus including fungi that produce stipitate, profusely branched, multicellular asexual reproductive structures (propagules) on leaves and in culture. Three species have been described in the genus: Korunomyces terminaliae – the type species, K. prostratus and K. zapatensis. No molecular studies have ever been conducted to elucidate the phylogenetic placement of Korunomyces. Recently, DNA sequences were obtained from pure cultures of K. prostratus and K. terminaliae, enabling an elucidation of their taxonomic placement. Isolates of K. prostratus obtained from diseased tissues of Miconia calvescens were observed for the first time to form pycnidial conidiomata in culture. A multi-gene phylogeny, including the large subunit of the nrDNA (nc LSU rDNA), internal transcribed spacer (ITS) region, polymerase II second largest subunit (RPB2) and translation elongation factor 1-α (TEF1), placed K. prostratus and K. terminaliae within Coniella (Schizoparmaceae). As Korunomyces is younger than Coniella, it is reduced to synonymy, and a new name and a new combination are proposed for these two species, namely: Coniella ferreirense nom. nov. and Coniella prostrata comb. nov. An emended description of Coniella to include the occasional formation of distinct and elaborate asexual propagules is also provided.

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