scholarly journals Formula Fertilization Promotes Phoebe bournei Robust Seedling Cultivation

Forests ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 781
Author(s):  
Zhi-Jian Yang ◽  
Xiao-Hui Wu ◽  
Steven C. Grossnickle ◽  
Lai-He Chen ◽  
Xin-Xiao Yu ◽  
...  

Phoebe bournei is a rare and endangered woody species and the success of its plantation development is dependent upon proper seedling cultivation. This study explored the regulation of N, P and K fertilizer and the interaction of these macronutrients on the growth of Phoebe bournei seedlings. To determine the optimum rate and ratio of N–P–K fertilizer in seedling cultivation, we used the unique “3414” incomplete orthogonal regression design to evaluate the effects of N–P–K fertilization on seedling morphological development. One-year-old Phoebe bournei bareroot seedlings were grown for one growing season under the defined fertilization regime, with their morphological development determined by measuring seedling attributes—root, stem, leaves and total biomass, root collar diameter and seedling height. These attributes were then combined to calculate the following indices: height-diameter ratio, shoot-root ratio and the seedling quality index (QI). Results showed that the N–P–K fertilizer had significant and beneficial effect on seedling cultivation. N effect was highest, followed by K and P. The three-way N×P×K interaction effect was strong, and the two-way interactions effect was highest for N×P, followed by P×K and N×K. At the “2” level of N (0.532 g·plant−1), P (P2O5, 0.133 g·plant−1), and K fertilizer (K2O, 0.356 g·plant−1), seedling growth and biomass accumulation were at their maximum. Unary, binary, and ternary quadratic fertilizer effect function equations of QI were established. Through comparative analysis, the ternary quadratic model was the optimal model and through a simulation–optimization, the optimal N–P–K fertilizer rates were 0.373~0.420 g·plant−1 (N), 0.086~0.106 g·plant−1 (P2O5), 0.243~0.280 g·plant−1 (K2O), with a N–P–K ratio of 1:0.20:0.43~1:0.65:0.75.

Author(s):  
Muhammad Zeeshan Mehmood ◽  
Ghulam Qadir ◽  
Obaid Afzal ◽  
Atta Mohi Ud Din ◽  
Muhammad Ali Raza ◽  
...  

AbstractSeveral biotic and abiotic stresses significantly decrease the biomass accumulation and seed yield of sesame crops under rainfed areas. However, plant growth regulators (such as Paclobutrazol) can improve the total dry matter and seed production of the sesame crop. The effects of the paclobutrazol application on dry matter accumulation and seed yield had not been studied before in sesame under rainfed conditions. Therefore, a two-year field study during 2018 and 2019 was conducted with key objectives to assess the impacts of paclobutrazol on leaf greenness, leaf area, total dry matter production and partitioning, seed shattering, and seed yield of sesame. Two sesame cultivars (TS-5 and TS-3) were treated with four paclobutrazol concentrations (P0 = Control, P1 = 100 mg L−1, P2 = 200 mg L−1, P3 = 300 mg L−1). The experiment was executed in RCBD-factorial design with three replications. Compared with P0, treatment P3 improved the leaf greenness of sesame by 17%, 38%, and 60% at 45, 85, and 125 days after sowing, respectively. However, P3 treatment decreased the leaf area of sesame by 14% and 20% at 45 and 85 days after sowing than P0, respectively. Compared with P0, treatment P3 increased the leaf area by 46% at 125 days after sowing. On average, treatment P3 also improved the total biomass production by 21% and partitioning in roots, stems, leaves, capsules, and seeds by 23%, 19%, 23%, 22%, and 40%, respectively, in the whole growing seasons as compared to P0. Moreover, under P3 treatment, sesame attained the highest seed yield and lowest seed shattering by 27% and 30%, respectively, compared to P0. This study indicated that by applying the paclobutrazol concentration at the rate of 300 mg L−1 in sesame, the leaf greenness, leaf areas, biomass accumulation, partitioning, seed yield, and shatter resistance could be improved. Thus, the optimum paclobutrazol level could enhance the dry matter accumulation and seed production capacity of sesame by decreasing shattering losses under rainfed conditions.


2020 ◽  
Vol 48 (2) ◽  
pp. 572-587
Author(s):  
Wenting XU ◽  
Miao ZHANG ◽  
Chen WANG ◽  
Xiongzhen LOU ◽  
Xiao HAN ◽  
...  

Phoebe bournei, a plant species endemic to China, is a precious timber tree and widely used in landscaping. This tree contains numerous secondary metabolites, underscoring its potential economic value. However, studies on this species, including molecular genetic research, remain limited. In this study, both a somatic embryogenesis (SE) technical system and Agrobacterium-mediated genetic transformation were successfully employed in P. bournei for the first time. The SE technical system was constructed using immature embryos as original material. The primary embryo and embryonic callus induction rates were 30.66% and 41.67%, respectively. The highest rate of embryonic callus proliferation was 3.84. The maximum maturity coefficient and germination rate were 53.44/g and 39%, respectively. Agrobacterium-mediated genetic transformation was performed using the SE technical system, and the highest transformation rate was 11.24%. The results presented here are the first to demonstrate an efficient approach to achieve numerous P. bournei plantlets, which serves as the basis for artificial cultivation and resource conservation. Furthermore, the genetic transformation platform constructed in this study will facilitate assessment of gene function and molecular regulation.


2020 ◽  
Vol 12 (6) ◽  
pp. 23
Author(s):  
Yuxin He ◽  
Chao Liu ◽  
Heping Xie ◽  
Jingchen Wang ◽  
Yang Wang ◽  
...  

Agriculture is a crucial area to be considered when exploring and exploiting the use of deep-underground space. We investigated the feasibility of deep-underground seed storage by keeping canola seed in either envelopes or sealed packages at four depths below the Earth’s surface (0, 240, 690, and 1410 m) at a gold mine in northeastern China. We studied the effects of storage depth and duration by conducting germination tests with the stored seed. The results showed that the rate of germination was reduced in seed stored at deeper levels and was also lower at all depths after a more prolonged period of storage. Seeds from sealed packages exhibited better resistance to the deep-underground environment than seeds kept in envelopes. However, measurements of hypocotyl lengths and biomass accumulation revealed that the germination of seeds stored in deep-underground was initially inhibited but recovered well compared with the control as the storage depth increased. The total biomass of the hypocotyl increased as the depth of seed storage deepened, indicating the existence of a compensatory effect on seed germination. The findings suggest that short-term deep-underground storage of seeds in sealed packages would improve the germination performance of cultivated canola in terms of the hypocotyl length and biomass accumulation and might be considered as a pre-sowing strategy.


Author(s):  
Petr Salaš

Reserve, slow-release fertilizers (SRF) enable to simplify the whole system of plant nutrition and fertilisation. Tabletted fertilizers of the Silvamix series represent a prospective product of Czech provenience. At our university, these fertilizers have been tested and used since the year 1991. Ornamental woody species grown in containers were investigated in two stages. Experiments with ornamental plants were established using one-year-old cuttings and seedlings of the following deciduous and evergreen woody species:Cotoneaster dammeri Skogholm,Berberis thunbergii,Potentilla fruticosa Snowflake,Ligustrum vulgare AtrovirensandPicea omorika. After planting into containers, fertilizers in the dose of 1 tablet (i.e. 10 g) per litre of substrate were applied either to roots level or on the soil surface in the container. Silvamix in the dose of 5 g.l-1was used as the tested fertilizer in the second stage. It was applied during the planting in the form of tablets and/or a powder. Control plants were fertilized in the course of growing season using a common agricultural fertilizer Cererit Z. The annual plants increments were measured. These experiments demonstrated a long-term optimum effect of this product on woody species and an equal quality and efficiency of its tabletted and powdered forms.


Author(s):  
Huan Pablo de Souza ◽  
Mauro Valdir Schumacher ◽  
Aline Aparecida Ludvichak ◽  
Dione Richer Momolli ◽  
Claudiney Do Couto Guimarães ◽  
...  

The objective of this study was to quantify the biomass and the macronutrient stock in an experiment of fertilization with Eucalyptus urophylla, planted in arenized soil at 12 months-old, in Rio Grande do Sul, Brazil. The experiment had a completely randomized design with five treatments (T1, T2, T3, T4 and T5) with three replications. The treatments T2, T3, T4 and T5, received increasing doses of triple superphosphate. On the other hand, the T1 treatment was the only one to receive natural phosphate in planting. For the determination of the biomass, fifteen trees were felled and separated in the following components: leaves, branches, stem bark, stem wood and roots. Samples of the components were collected and transported to the laboratory for biomass determination and chemical analysis. The total biomass varied between the treatments, with highest biomass accumulation of 6.83 Mg ha-1 occurring in T5. The T1 presented the highest biomass for roots representing 33.4% of the total biomass. The biomass distribution among the different components in the decreasing order was: roots > stem wood > leaves > branches > stem bark, for all the treatments. The treatment with higher doses of fertilizers (T5) presented the highest amount of nutrient accumulation in the total biomass (131.26 kg ha-1). The concentration and accumulation of nutrients presented the following trend K > N > Ca > Mg > P > S. Analyzing the different components of biomass, the highest amounts of nutrients followed the order: leaves > roots > stem wood > branches > stem bark. Fertilization influenced the biomass production of E. urophylla in arenized soil in the Pampa biome, but without significant differences to date (12 months). The leaves had the highest concentration of macronutrients, with the exception of Ca, which was higher in the bark. The K was the element that presented highest accumulation in whole tree. The plantation of eucalyptus with fertilization management may be an alternative for the economic use of arenized soil.


2016 ◽  
Vol 25 (10) ◽  
pp. 1086 ◽  
Author(s):  
Kellie A. Uyeda ◽  
Douglas A. Stow ◽  
John F. O'Leary ◽  
Christina Tague ◽  
Philip J. Riggan

Chaparral wildfires typically create even-aged stands of vegetation that grow quickly in the first 2 decades following a fire. Patterns of this growth are important for understanding ecosystem productivity and re-establishment success, but are logistically challenging to measure over long time periods. We tested the utility of a novel method of using shrub growth rings to estimate stand-level biomass accumulation at an annual time scale in southern California chaparral. We examined how temporal variation in precipitation and spatial variation in solar irradiation influence that accumulation. Using field measurements and a relationship between stem basal area and aboveground biomass, we estimated current biomass levels in an 11-year-old chaparral stand, and used growth-ring diameters to estimate growth in each year from age 4 to 11 years. We found that annual growth as measured by shrub growth rings tracked closely with patterns of annual precipitation, but not with time since fire. Solar irradiation was not found to be a significant covariate with total biomass by plot, possibly due to sampling area limitations. The close relationship of annual biomass accumulation with annual precipitation indicates that shrub growth-ring measurements can provide a useful metric of stand-level recovery.


2005 ◽  
Vol 40 (4) ◽  
pp. 390-400 ◽  
Author(s):  
Allen C. Cohen ◽  
Fanrong Zeng ◽  
Patrick Crittenden

Bioassays were conducted to examine the effects of raw soybean extract in artificial diet on the development, survival, adult weight and biomass accumulation of Lygus hesperus Knight. The diet containing raw soybean extract significantly reduced survival of L. hesperus. Total biomass per rearing unit and survival from eggs to adults were significantly less for L. hesperus fed diet containing raw soybean extract than it was for those fed heat-treated extract, diet with extraction buffer but no extract, or control (standard) diet. Development period, weight of individual adults, and egg production were not significantly different among the four treatments. However, development time was greater for the L. hesperus exposed to raw soy extract, and egg production, as well as individual adult weights, were consistently lower than those fed on the other treatments. Raw and autoclaved soy extracts were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), revealing differences in protein banding patterns, including those from total protein and glycoprotein profiles. Activity of soy trypsin inhibitor (STI) also was measured in raw and heated samples, and it was determined that autoclaving greatly decreased the inhibition activity of this protein. The heat-based deactivation of STI, and the disappearance of most protein bands are most likely associated with the denaturation of proteins and formation of large aggregates that fail to migrate in an electrophoretic field.


2000 ◽  
Vol 16 (5) ◽  
pp. 689-708 ◽  
Author(s):  
Marc K. Steininger

A study was conducted on the effect of extended land-use on secondary forest biomass accumulation in the Amazon. Structural measurements were made in a series of secondary forest stands, from 4–30 y old, in Brazil and Bolivia. Half of the stands were forest regrowth following clearance and only 1 y of cultivation; the other half were regrowth following 4 y or more of continuous pasture in Brazil and three or more rotations of medium-fallow agriculture in Bolivia. Above-ground live biomass was estimated using published allometric equations. Total biomass ranged from 17 to 207 Mg ha−1. Biomass of pioneer trees was poorly related to stand age, while that of later-successional trees increased linearly with age. Total biomass accumulation in Bolivia averaged 5.4 Mg ha−1 y−1 over the entire age sequence. Biomass accumulation for regrowth following short-term use was not greater than that for regrowth following medium-fallow agriculture. In Brazil, biomass accumulation averaged 9.1 Mg ha−1 y−1 over the first 12 y of regrowth and 5.9 Mg ha−1 y−1 over the entire age sequence. Biomass accumulation was significantly slower, around 5.0 Mg ha−1 y−1, for regrowth following continuous pasture than for regrowth following 1 y of cultivation.


2005 ◽  
Vol 4 (12) ◽  
pp. 1982-1989 ◽  
Author(s):  
Jarrod R. Fortwendel ◽  
Wei Zhao ◽  
Ruchi Bhabhra ◽  
Steven Park ◽  
David S. Perlin ◽  
...  

ABSTRACT The Ras family of GTPase proteins has been shown to control morphogenesis in many organisms, including several species of pathogenic fungi. In a previous study, we identified a gene encoding a fungus-specific Ras subfamily homolog, rasB, in Aspergillus fumigatus. Here we report that deletion of A. fumigatus rasB caused decreased germination and growth rates on solid media but had no effect on total biomass accumulation after 24 h of growth in liquid culture. The ΔrasB mutant had an irregular hyphal morphology characterized by increased branching. Expression of rasBΔ113-135, a mutant transgene lacking the conserved rasB internal amino acid insertion, did not complement the deletion phenotype of delayed growth and germination rates and abnormal hyphal morphology. Virulence of the rasB deletion strain was diminished; mice infected with this strain exhibited ∼65% survival compared to ∼10% with wild-type and reconstituted strains. These data support the hypothesis that rasB homologs, which are highly conserved among fungi that undergo hyphal growth, control signaling modules important to the directional growth of fungal hyphae.


2006 ◽  
Vol 24 (4) ◽  
pp. 213-217
Author(s):  
S.M. Scheiber ◽  
Richard C. Beeson

Abstract Begonias (Begonia semperflorens) were transplanted into an open-sided clear polyethylene covered shelter to evaluate the effect of root ball condition on establishment and growth. Three root ball classes were evaluated: non root-bound (NRB; 6 week old plants), root-bound (RB; 10 week old plants), and root-bound with the bottom 1 cm of the root ball removed (RBM). Non root-bound plants had greater growth rates for both height and faster rates of accumulation for cumulative root dry mass and new root dry mass relative to the other treatments tested. Cumulative shoot dry mass, new shoot dry mass, and total biomass accumulation rates were slower among RB plants compared to other rootball conditions. Mean canopy size, shoot dry mass, and biomass of NRB were significantly less at transplant; however all parameters were comparable among treatments 12 weeks later. Final mean shoot to root ratios were lower for the NRB treatment relative to RBM. Results indicate smaller, NRB transplants establish faster in the landscape. Furthermore, rootball manipulation is not recommended as it had no significant effect on root establishment or canopy growth of this annual bedding plant in the landscape.


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