scholarly journals Effects of Hormones and Epigenetic Regulation on the Callus and Adventitious Bud Induction of Fraxinus mandshurica Rupr.

Forests ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 590
Author(s):  
Lei Yu ◽  
Xinyu Li ◽  
Hongmei Tian ◽  
Hualing Liu ◽  
Ying Xiao ◽  
...  
Keyword(s):  
Callus Induction ◽  
Adventitious Bud ◽  
Stem Explants ◽  
Adventitious Buds ◽  
Regeneration System ◽  
Fraxinus Mandshurica ◽  
Asexual Propagation ◽  
Bud Induction ◽  
Callus Regeneration ◽  
Adventitious Bud Induction

Fraxinus mandshurica Rupr. (hereafter “F. mandshurica”) is known as one of northeast China′s important, valuable hardwood timber species. However, tissue culture and micropropagation of the species are difficult and have low efficiency, limiting asexual propagation. In this manuscript, stem explants were utilized to establish an effective regeneration system through adventitious bud organogenesis. The factors influencing callus regeneration in vitro were determined, and callus regeneration technology was established. The mechanism of adventitious bud formation was analyzed. Thidiazuron (TDZ) played a crucial role in the formation of adventitious buds. Elevated concentrations of TDZ were beneficial to callus induction and low concentrations of 6-benzyladenine (BA) led to loose state callus formation. The order of callus induction rates for different explants was stem cotyledon (100%) > segment (98.54%) > hypocotyl (92.56%) > root (50.71%). The effects of exogenous addition of 6-BA and TDZ on the endogenous hormone content of plants during the regeneration of adventitious buds were also assessed, as well as the expression characteristics of genes related to the regeneration pathway. The comprehensive analysis results showed that the suitable medium for callus induction and adventitious bud differentiation was c12 medium (MSB5 + 30 g/L sucrose + 7 g/L Agar + 5 mg/L 6-BA + 8 mg/L TDZ + 2 mg/L glycine + 0.1 mg/L IBA + 5% coconut water). The induction rates of callus and adventitious buds were 99.15% and 33.33%. The addition of 2.4 mg/L of the DNA demethylation reagent 5-azacytidine (5-aza) and 0.15 mg/L of the histone deacetylase inhibitor trichostatin A (TSA) increased the rates of adventitious bud induction by 17.78% over the control. This further laid the foundation for large-scale cultivation of excellent varieties and genetic transformation techniques.

scholarly journals Effects of different factors on adventitious bud induction from stem explants of Ludisia discolor

2021 ◽  
Vol 245 ◽  
pp. 03024
Author(s):  
Ying Liu ◽  
Xiaohao Li ◽  
Jingye Chen ◽  
Yingbin Xue ◽  
Yinling Zhu
Keyword(s):  
Tissue Culture ◽  
Theoretical Basis ◽  
Culture Conditions ◽  
Adventitious Bud ◽  
Stem Explants ◽  
Regeneration Rate ◽  
Bud Regeneration ◽  
Bud Induction ◽  
Adventitious Bud Regeneration ◽  
Adventitious Bud Induction

In this study, the stem explants of Ludisia discolor were used as experimental materials to investigate the effects of 6-BA, NAA, Cu2+ and Ag+ on the induction of adventitious bud regeneration, and to analyze the most suitable culture conditions for stem explants regeneration. The results showed that when the medium was supplemented with 1.0 mg/L 6-BA, 0.75 mg/L NAA, 0.25 mg/L CuSO4, or 6.4 mg/L AgCl, the best regeneration effects would be gained, respectively. And the adventitious bud regeneration rate reached the maximum, which were 61.67%, 83.67%, 80.95% and 87.63%, respectively. The results of this study provided a theoretical basis for tissue culture and rapid propagation of L. discolor.

ZYGOTIC EMBRYO CULTURE OF TAXUS CHINESIS VAR. MAIREI AND PLANT REGENERATION THROUGH ORGANOGENESIS

1999 ◽  
Vol 47 (4) ◽  
pp. 287-289 ◽  
Author(s):  
Deyu Xie ◽  
Zhongchen Guo
Keyword(s):  
Plant Regeneration ◽  
Embryo Culture ◽  
Adventitious Roots ◽  
Zygotic Embryo ◽  
Adventitious Bud ◽  
Ms Medium ◽  
Adventitious Buds ◽  
Zygotic Embryo Culture ◽  
Bud Induction ◽  
Adventitious Bud Induction

When different media were compared for their effect on zygotic embryo culture, DCR was found more effective than MS medium. From these embryos, adventitious bud induction was achieved by optimizing formular conditions as described in this paper. Adventitious buds were formed from calli derived from hypocotyls of germinated embryos. Finally, adventitious roots were induced and complete plantlets were obtained.

scholarly journals Basal stem cluster bud induction and efficient regeneration for the Tibetan endemic medicinal plant Swertia conaensis

2021 ◽  
Vol 49 (2) ◽  
pp. 12152
Author(s):  
Yin-Kai XI ◽  
Heng-Yu HUANG
Keyword(s):  
Plant Regeneration ◽  
Medicinal Plant ◽  
Orthogonal Experiment ◽  
Occurrence Rate ◽  
Adventitious Bud ◽  
Ms Medium ◽  
Regeneration System ◽  
Bud Induction ◽  
Endemic Medicinal Plant

The artificial rapid propagation system for Swertia conaensis T. N. Ho et S. W. Liu was explored to screen the appropriate plant regeneration method and to provide an efficient propagation mode, useful for artificial breeding technology or for further research and development of the Tibetan endemic medicinal plant. In this study, the most suitable explant and hormone were chosen according to single factor test. Next, the effects of different hormone combinations on basal stem cluster bud induction, callus induction, adventitious bud occurrence and plant regeneration were investigated by using complete combination and orthogonal experiment. The obtained results showed that the explants suitable for in vitro of S. conaensis were stem tips with leaves, which were regenerated through the method of basal stem cluster bud occurrence in the MS medium with 2.0 mg∙L-1 6-BA, 0.5 mg∙L-1 NAA, but the proliferation coefficient was low, only 3.16 after 40 days of culture. Subsequently, the proliferation coefficient failed to improve, irrespective of change of the concentration ratio of 6-BA and NAA. Therefore, in the orthogonal experiment of adding ZT, the MS medium with 1.0 mg∙L-1 ZT, 0.5 mg∙L-1 NAA and 2.5 mg∙L-1 6-BA induced a large number of callus green and compact, with 86.30% callus occurrence rate. After 40 days of culture, the rate of adventitious bud occurrence was 96.55% and the proliferation coefficient was high (10.37). The rooting rate was 100% in the 1/2MS medium with 0.5 mg∙L-1 NAA. The survival rate of regenerated plants was more than 95%. Indirect organogenesis was more efficient than direct organogenesis in in vitro culture of S. conaensis. In this study, the efficient and stable regeneration system of S. conaensis was achieved through the method of explant to callus to adventitious buds, which provided an effective way to an endangered species.

scholarly journals Induction of Adventitious buds from stem explants in Aoectochilus formosanus

2019 ◽  
Vol 131 ◽  
pp. 01131
Author(s):  
Yingbin Xue ◽  
Fengyi Xiao ◽  
Xiaohao Li ◽  
Huamei Chen ◽  
Gangshun Rao ◽  
...  
Keyword(s):  
Culture Conditions ◽  
Adventitious Bud ◽  
Naphthaleneacetic Acid ◽  
Stem Explants ◽  
Ms Medium ◽  
Adventitious Buds ◽  
High Concentration ◽  
Diethyl Aminoethyl Hexanoate ◽  
Adventitious Bud Regeneration ◽  
Stem Segments

In order to explore the optimal culture conditions for adventitious bud regeneration of stem explants in Aoectochilus formosanus, the stem segments from the sterile seedlings were used as explants, and different concentrations of diethyl aminoethyl hexanoate (DA-6), kinetin (KT), Cu2+ and glutamine (Gln) were separately added into MS medium containing 6-benzylaminopurine (6-BA) and naphthaleneacetic acid (NAA), and the induction rate and the induction multiple of adventitious buds were recorded and analyzed. The results showed that the regeneration of adventitious buds could be promoted, when 2 mg/L DA-6, 0.4 mg/L KT and 15 mg/L Gln were added in mediums. However, the effect of Cu2+ on the regeneration of adventitious buds in A. formosanus was enhanced by low concentration and suppressed by high concentration, and the best concentration of Cu2+ was 5 mg/L.

Adventitious bud induction in Eucalyptus globulus Labill

2001 ◽  
Vol 37 (3) ◽  
pp. 388-391 ◽  
Author(s):  
Greg Nugent ◽  
Stephen F. Chandler ◽  
Phil Whiteman ◽  
Trevor W. Stevenson
Keyword(s):  
Eucalyptus Globulus ◽  
Adventitious Bud ◽  
Bud Induction ◽  
Adventitious Bud Induction
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