scholarly journals Arbuscular Mycorrhizal Fungi Mitigate Nitrogen Leaching under Poplar Seedlings

Forests ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 325
Author(s):  
Fengru Fang ◽  
Chunyan Wang ◽  
Fei Wu ◽  
Ming Tang ◽  
Russell Doughty
Keyword(s):  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Root Surface ◽  
Root Surface Area ◽  
N Leaching ◽  
Soil N ◽  
Total N ◽  
N Level ◽  
N Concentration

The leaching of soil nitrogen (N) has become one of the most concerning environmental threats to ecosystems. Arbuscular mycorrhizal (AM) fungi have important ecological functions, however, their influence on soil N leaching and the mechanism of action remain unclear. We conducted a two-factor (N application level × AM inoculation) experiment on poplar, and for the first time, comprehensively analyzed the mechanism by which AM fungi influence soil N leaching. The results showed that, under optimum (7.5 mM) and high (20 mM) N levels, the nitrate (NO3−) and ammonium (NH4+) concentrations of leachate in the AM inoculated treatment (+AM) were lower than in the non-inoculated treatment (−AM), with significant reductions of 20.0% and 67.5%, respectively, under high N level, indicating that AM inoculation can reduce soil N leaching and that it is more effective for NH4+. The arbuscular and total colonization rates gradually increased, and the morphology of spores and vesicles changed as the N level increased. Under optimum and high N levels, +AM treatment increased the root N concentration by 11.7% and 50.7%, respectively; the increase was significant (p < 0.05) at the high N level, which was associated with slightly increased transpiration and root activity despite reductions in root surface area and root length. Additionally, the +AM treatment increased soil cation exchange capacity (CEC), soil organic carbon (SOC), and significantly (p < 0.05) increased the proportions of macroaggregates (but without significant change in microaggregates), causing soil total nitrogen (TN) to increase by 7.2% and 4.7% under optimum and high N levels, respectively. As the N levels increased, the relative contributions of AM inoculation on N leaching increased, however, the contributions of plant physiological and soil variables decreased. Among all of the variables, SOC had important contributions to NH4+ and total N in the leachate, while root N concentration had a higher contribution to NO3−. In conclusion, AM fungi can mitigate soil N leaching and lower the risk of environmental pollution via enhancing N interception by the inoculated fungi, increasing N sequestration in plant roots, and by improving soil N retention.

scholarly journals Response of growth and drought tolerance of Acacia seyal Del. seedlings to arbuscular mycorrhizal fungi

2020 ◽  
Vol 66 (No. 6) ◽  
pp. 264-271 ◽  
Author(s):  
Abdalla Ahmed ◽  
Abdelmalik Abdelmalik ◽  
Thobayet Alsharani ◽  
Bdulaziz Al-Qarawi Al-Qarawi ◽  
Ibrahim Aref
Keyword(s):  
Drought Tolerance ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Glomus Intraradices ◽  
Arbuscular Mycorrhizal ◽  
Arid Environment ◽  
Root Surface ◽  
Root Surface Area ◽  
Root Tips ◽  
Acacia Seyal

Considering the improvement of acacia species growth in arid and semi-arid environment, a pot experiment was conducted to evaluate the role of arbuscular mycorrhizal fungi (AMF); Funneliformis mosseae (syn. Glomus mosseae), Rhizophagus intraradices (syn. Glomus intraradices) and Claroideoglomus etunicatum (syn. Glomus etunicatum) on growth and drought tolerance of Acacia seyal Del. seedlings under drought cycles (7, 14, 21 and 28 days). AMF-inoculated seedlings showed a clear colonisation percentage (36–68%). AMF treatment significantly improved seedlings shoot and root growth under all drought cycles compared to non-AMF control seedlings. Moreover, AMF treatment enhanced seedlings drought resistance by increasing root surface area (root length increased by 483.76% and root tips number increased by 1 463.94% under 28 days of drought cycle), there was a strong linear relation between proline accumulation, AMF and drought stress (proline content decreased in treated seedlings by 31.3% and 14.3% and increased by 97.5% and 80.4% in untreated seedlings under drought cycles of 21 and 28 days, respectively). In conclusion, the AMF inoculation improved growth and enhanced drought tolerance of A. seyal seedlings and can be used as a natural biostimulator for acacias seedlings establishment in arid areas.

scholarly journals Improvement of Root System Architecture in Peach (Prunus persica) Seedlings by Arbuscular Mycorrhizal Fungi, Related to Allocation of Glucose/Sucrose to Root

2011 ◽  
Vol 39 (2) ◽  
pp. 232 ◽  
Author(s):  
Qiang-Sheng WU ◽  
Guo-Huai LI ◽  
Ying-Ning ZOU
Keyword(s):  
Root System ◽  
System Architecture ◽  
Root Length ◽  
Mycorrhizal Fungi ◽  
Prunus Persica ◽  
Arbuscular Mycorrhizal ◽  
Root Surface ◽  
Root System Architecture ◽  
Root Surface Area ◽  
Projected Area

Root system architecture (RSA) is used to describe the spatial configuration of a root system in the soil, which substantially determines the capacity of a plant to take up nutrients and water. The present study was to assess if arbuscular mycorrhizal fungi (AMF), Glomus mosseae, G. versiforme, and Paraglomus occultum would alter RSA of peach (Prunus persica L. Batsch) seedlings, and the alteration due to mycorrhization was related to allocation of glucose/sucrose to root (Aglucose/sucrose). Inoculation with G. mosseae and G. versiforme significantly increased leaf, stem, root and total fresh weights, compared with non-AMF treatment. Mycorrhizal alterations of RSA in peach plants were dependent on AMF species, because only G. mosseae and G. versiforme but not P. occultum markedly increased root length, root projected area, root surface area and root volume. For the distribution of root length classes, AMF mainly increased 0-1 and 3-4 cm root length classes, which is AMF species dependent. Inoculated seedlings with Glomus species recorded significantly higher root sucrose and leaf and root glucose concentrations and lower root sucrose concentrations than un-inoculated control. Compared with the non-AMF treatment, G. mosseae and G. versiforme generally increased the Aglucose and Asucrose, but P. occultum significantly decreased the Aglucose and Asucrose. Asucrose or Aglucose was significantly positive correlated with root length, root projected area and root surface area. The results suggest that AMF modified variables of RSA in peach, which is AMF species dependent and related to Aglucose and Asucrose.

Long-term effects on soil-water nitrogen and pH of clearcutting and simulated disc trenching of previously nitrogen-fertilised pine plots

2018 ◽  
Vol 48 (10) ◽  
pp. 1115-1123
Author(s):  
Eva Ring ◽  
Lars Högbom ◽  
Staffan Jacobson ◽  
Gunnar Jansson ◽  
Hans-Örjan Nohrstedt
Keyword(s):  
Soil Water ◽  
Site Preparation ◽  
Low Fertility ◽  
N Leaching ◽  
Soil N ◽  
Long Term Effects ◽  
Total N ◽  
N Concentration ◽  
Experimental Plots

Forest fertilisation with nitrogen (N) typically increases N leaching for 1–2 years. Some studies have reported effects also after clearcutting. This study presents an analysis of soil-water chemistry data from the 3rd to the 15th year after clearcutting of fertilised experimental plots on a low-fertility site in Sweden. Before clearcutting in 1987, study plots had been fertilised with NH4NO3 in 1967, 1974, and 1981, resulting in total applications ranging from 0 to 1800 kg N·ha−1. In 1989, disc trenching was simulated by manual digging on small subplots within the fertilised main plots. Soil-water samples were collected at a depth of 50 cm. Previous N fertilisation and site preparation, respectively, affected (p < 0.05) the total N and NO3–-N concentrations and pH of soil water, but no statistical interaction between fertilisation and site preparation was found. The NO3–-N concentration was elevated for total N applications above 720 kg·ha−1 (mean NO3–-N concentration of 0.93 mg·L−1 for 1080 kg N·ha−1, 1.6 mg·L−1 for 1440 kg N·ha−1, and 2.4 mg·L−1 for 1800 kg N·ha−1 compared with 0.20 mg·L−1 for the control) and lower after simulated disc trenching (0.63 mg·L−1) than in nontrenched soil (1.3 mg·L−1). The elevations in the soil-water NO3–-N concentration for the fertiliser treatments seemed to be related to changes in the soil N store created by previous fertilisation.

scholarly journals Slash-and-Burn Practices Decrease Arbuscular Mycorrhizal Fungi Abundance in Soil and the Roots of Didierea madagascariensis in the Dry Tropical Forest of Madagascar

Fire ◽  
2018 ◽  
Vol 1 (3) ◽  
pp. 37
Author(s):  
Alícia Barraclough ◽  
Pål Olsson
Keyword(s):  
Low Income ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Total N ◽  
Slash And Burn ◽  
Carbon And Nitrogen ◽  
Fungal Abundance ◽  
Am Symbiosis ◽  
Network Disruption

Deforestation and the use of fire to clear land have drastic effects on ecosystem functioning and compromise essential ecosystem services, especially in low-income tropical countries such as Madagascar. We evaluated the effects of local slash-and-burn practices on soil nutrients and arbuscular mycorrhizal (AM) fungi abundance in a southwestern Madagascar forest. Nine sampling plot pairs were established along the border of a reserve within the Fiherenana–Manombo (pk-32) complex, where soil and seedling root samples of the endemic tree Didierea madagascariensis were taken. We analysed soil extractable PO43−, NH4+, and NO3− as well as total soil carbon and nitrogen. We analysed AM fungal abundance in soil and roots through fatty acid marker analysis (NLFA and PLFA 16:1ω5), spore extraction, and root staining. Slash-and-burn caused an increase in pH and doubled the plant available nutrients (from 7.4 to 13.1 µg PO43− g−1 and from 6.9 to 13.2 µg NO3− g−1). Total C and total N increased in deforested soil, from 0.6% to 0.84% and from 0.06% to 0.08%, respectively. There was a significant decline in AM fungi abundance in soil, with a decrease in soil NLFA 16:1ω5 from 0.2 to 0.12 nmol/g. AM fungi abundance in D. madagascariensis roots was also negatively affected and colonization decreased from 27.7% to 16.9% and NLFA 16:1ω5 decreased from 75.7 to 19 nmol/g. Together with hyphal network disruption, increased nutrient availability caused by burning is proposed as an explanation behind AM decline in soil and roots of D. madagascariensis. This is the first study to report the effects of slash-and-burn on AM symbiosis in Madagascar’s dry forests, with likely implications for other tropical and subtropical dryland forests worldwide where slash-and-burn is practiced.

scholarly journals Symbiotic relationship of am fungi with root of phyllanthus amarus in western ghats, of tamil nadu

2021 ◽  
Vol 8 (1) ◽  
pp. 65-68
Author(s):  
Venkatachalapathi A ◽  
Nagarajan N
Keyword(s):  
Mycorrhizal Fungi ◽  
Western Ghats ◽  
Tamil Nadu ◽  
Higher Plants ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Root Surface ◽  
Symbiotic Relationship ◽  
Phyllanthus Amarus ◽  
Root Tissues

The mutually association between a fungus and root of a higher plants are called as Mycorrhiza, when the fungal hyphae live on root surface is called Ectomycorrhiza and when penetrate the root and grows inside the root tissue is called Endomycorrhiza. The different type of Arbuscular Mycorrhizal fungi (AMF) were evaluated for the symbiotic relationship with Phyllanthus amarus in Western Ghats. Glomus fasciculatum, Gigospora margarita, etc., are isolated in the forest rhizosphere soil. In the presence of AMF in root tissues generally had greater plant height, biomass, stem diameter, number of leaves and phosphorus content where rich in amount compared to other plant species.

scholarly journals Effects of two wood-based biochars on the fate of added fertilizer nitrogen—a 15N tracing study

2021 ◽  
Author(s):  
Subin Kalu ◽  
Gboyega Nathaniel Oyekoya ◽  
Per Ambus ◽  
Priit Tammeorg ◽  
Asko Simojoki ◽  
...  
Keyword(s):  
Plant Uptake ◽  
Plant Biomass ◽  
Application Rate ◽  
Control Treatment ◽  
Organic N ◽  
N Leaching ◽  
Soil N ◽  
Mineral N ◽  
Total N ◽  
Application Rates

AbstractA 15N tracing pot experiment was conducted using two types of wood-based biochars: a regular biochar and a Kon-Tiki-produced nutrient-enriched biochar, at two application rates (1% and 5% (w/w)), in addition to a fertilizer only and a control treatment. Ryegrass was sown in pots, all of which except controls received 15N-labelled fertilizer as either 15NH4NO3 or NH415NO3. We quantified the effect of biochar application on soil N2O emissions, as well as the fate of fertilizer-derived ammonium (NH4+) and nitrate (NO3−) in terms of their leaching from the soil, uptake into plant biomass, and recovery in the soil. We found that application of biochars reduced soil mineral N leaching and N2O emissions. Similarly, the higher biochar application rate of 5% significantly increased aboveground ryegrass biomass yield. However, no differences in N2O emissions and ryegrass biomass yields were observed between regular and nutrient-enriched biochar treatments, although mineral N leaching tended to be lower in the nutrient-enriched biochar treatment than in the regular biochar treatment. The 15N analysis revealed that biochar application increased the plant uptake of added nitrate, but reduced the plant uptake of added ammonium compared to the fertilizer only treatment. Thus, the uptake of total N derived from added NH4NO3 fertilizer was not affected by the biochar addition, and cannot explain the increase in plant biomass in biochar treatments. Instead, the increased plant biomass at the higher biochar application rate was attributed to the enhanced uptake of N derived from soil. This suggests that the interactions between biochar and native soil organic N may be important determinants of the availability of soil N to plant growth.

scholarly journals Use of the Signature Fatty Acid 16:1ω5 as a Tool to Determine the Distribution of Arbuscular Mycorrhizal Fungi in Soil

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Christopher Ngosong ◽  
Elke Gabriel ◽  
Liliane Ruess
Keyword(s):  
Fatty Acid ◽  
Mycorrhizal Fungi ◽  
Plant Root ◽  
Background Level ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Biomass Estimation ◽  
Lipid Fatty Acid ◽  
Neutral Lipid Fatty Acid ◽  
Root Symbionts

Biomass estimation of arbuscular mycorrhiza (AM) fungi, widespread plant root symbionts, commonly employs lipid biomarkers, predominantly the fatty acid 16:1ω5. We briefly reviewed the application of this signature fatty acid, followed by a case study comparing biochemical markers with microscopic techniques in an arable soil following a change to AM non-host plants after 27 years of continuous host crops, that is, two successive cropping seasons with wheat followed by amaranth. After switching to the non-host amaranth, spore biomass estimated by the neutral lipid fatty acid (NLFA) 16:1ω5 decreased to almost nil, whereas microscopic spore counts decreased by about 50% only. In contrast, AM hyphal biomass assessed by the phospholipid (PLFA) 16:1ω5 was greater under amaranth than wheat. The application of PLFA 16:1ω5 as biomarker was hampered by background level derived from bacteria, and further enhanced by its incorporation from degrading spores used as microbial resource. Meanwhile, biochemical and morphological assessments showed negative correlation for spores and none for hyphal biomass. In conclusion, the NLFA 16:1ω5 appears to be a feasible indicator for AM fungi of the Glomales group in the complex field soils, whereas the use of PLFA 16:1ω5 for hyphae is unsuitable and should be restricted to controlled laboratory studies.

scholarly journals Response of Arbuscular Mycorrhizal Fungi to Simulated Climate Changes by Reciprocal Translocation in Tibetan Plateau

2015 ◽  
Vol 43 (2) ◽  
pp. 488-493
Author(s):  
Zhaoyong SHI ◽  
Xubin YIN ◽  
Bede MICKAN ◽  
Fayuan WANG ◽  
Ying ZHANG ◽  
...  
Keyword(s):  
Tibetan Plateau ◽  
Arbuscular Mycorrhizal Fungi ◽  
Reciprocal Translocation ◽  
Mycorrhizal Fungi ◽  
Climate Changes ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Spore Density ◽  
The Tibetan Plateau ◽  
Colonization Frequency

Arbuscular mycorrhiza (AM) fungi are considered as an important factor in predicting plants and ecosystem responses to climate changes on a global scale. The Tibetan Plateau is the highest region on Earth with abundant natural resources and one of the most sensitive region to climate changes. To evaluate the complex response of arbuscular mycorrhizal fungi colonization and spore density to climate changes, a reciprocal translocation experiment was employed in Tibetan Plateau. The reciprocal translocation of quadrats to AM colonization and spore density were dynamic. Mycorrhizal colonization frequency presented contrary changed trend with elevations of quadrat translocation. Colonization frequency reduced or increased in majority quadrats translocated from low to high or from high to low elevation. Responses of colonization intensity to translocation of quadrats were more sensitive than colonization frequency. Arbuscular colonization showed inconsistent trend in increased or decreased quadrat. Vesicle colonization decreased with changed of quadrat from low to high elevations. However, no significant trend was observed. Although spore density was dynamic with signs of decreasing or increasing in translocated quadrats, the majority enhanced and declined respectively in descent and ascent quadrat treatments. It is crucial to understand the interactions between AM fungi and prairie grasses to accurately predict effects of climate change on these diverse and sensitive ecosystems. This study provided an opportunity for understanding the effect of climate changes on AM fungi.

scholarly journals Effect of Winter Cover Crops on Soil Nitrogen Availability, Corn Yield, and Nitrate Leaching

2001 ◽  
Vol 1 ◽  
pp. 22-29 ◽  
Author(s):  
S. Kuo ◽  
B. Huang ◽  
R. Bembenek
Keyword(s):  
Cover Crops ◽  
Cover Crop ◽  
N Fertilizer ◽  
N Leaching ◽  
Corn Yield ◽  
N Availability ◽  
Soil N ◽  
Soil N Availability ◽  
N Concentration ◽  
Fertilizer Rates

Biculture of nonlegumes and legumes could serve as cover crops for increasing main crop yield, while reducing NO3leaching. This study, conducted from 1994 to 1999, determined the effect of monocultured cereal rye (Secale cereale L.), annual ryegrass (Lolium multiflorum), and hairy vetch (Vicia villosa), and bicultured rye/vetch and ryegrass/vetch on N availability in soil, corn (Zea mays L.) yield, and NO3-N leaching in a silt loam soil. The field had been in corn and cover crop rotation since 1987. In addition to the cover crop treatments, there were four N fertilizer rates (0, 67, 134, and 201 kg N ha-1, referred to as N0, N1, N2, and N3, respectively) applied to corn. The experiment was a randomized split-block design with three replications for each treatment. Lysimeters were installed in 1987 at 0.75 m below the soil surface for leachate collection for the N0, N2, and N3treatments. The result showed that vetch monoculture had the most influence on soil N availability and corn yield, followed by the bicultures. Rye or ryegrass monoculture had either no effect or an adverse effect on corn yield and soil N availability. Leachate NO3-N concentration was highest where vetch cover crop was planted regardless of N rates, which suggests that N mineralization of vetch N continued well into the fall and winter. Leachate NO3-N concentration increased with increasing N fertilizer rates and exceeded the U.S. Environmental Protection Agency’s drinking water standard of 10 mg N l�1 even at recommended N rate for corn in this region (coastal Pacific Northwest). In comparisons of the average NO3-N concentration during the period of high N leaching, monocultured rye and ryegrass or bicultured rye/vetch and ryegrass/vetch very effectively decreased N leaching in 1998 with dry fall weather. The amount of N available for leaching (determined based on the presidedress nitrate test, the amount of N fertilizer applied, and N uptake) correlated well with average NO3-N during the high N leaching period for vetch cover crop treatment and for the control without the cover crops. The correlation, however, failed for other cover crops largely because of variable effectiveness of the cover crops in reducing NO3leaching during the 5 years of this study. Further research is needed to determine if relay cover crops planted into standing summer crops is a more appropriate approach than fall seeding in this region to gain sufficient growth of the cover crop by fall. Testing with other main crops that have earlier harvest dates than corn is also needed to further validate the effectiveness of the bicultures to increase soil N availability while protecting the water quality.

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