scholarly journals Widely Targeted Metabolomic and Transcriptomic Analyses of a Novel Albino Tea Mutant of “Rougui”

Forests ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 229
Author(s):  
Pengjie Wang ◽  
Yucheng Zheng ◽  
Yongchun Guo ◽  
Baoshun Liu ◽  
Shan Jin ◽  
...  
Keyword(s):  
Heat Shock ◽  
Yellow Leaf ◽  
Chloroplast Biogenesis ◽  
Heat Shock Transcription Factors ◽  
Metabolite Profiles ◽  
Leaf Coloration ◽  
Leaf Mutant ◽  
Metabolic Mechanism ◽  
Shock Proteins ◽  
Leaf Mutants

Albino tea mutants with specific shoot colors (white or yellow) have received increasing attention from researchers due to their unique phenotypes, beneficial metabolites, and special flavor. In this study, novel natural yellow leaf mutants of the same genetic background of “Rougui” were obtained, and the transcriptome and metabolite profiles of the yellow leaf mutant (YR) and original green cultivar (GR) were investigated. A total of 130 significantly changed metabolites (SCMs) and 55 differentially expressed genes (DEGs) were identified in YR compared to GR. The leaf coloration of YR was primarily affected by pigment metabolism including of chlorophyll, carotenoids, and flavonoids, and the co-expression of three heat shock proteins (HSPs) and four heat shock transcription factors (HSFs) may also regulate leaf coloration by affecting chloroplast biogenesis. Of the 130 SCMs, 103 showed clearly increased abundance in YR, especially nucleotides and amino acids and their derivatives and flavonoids, suggesting that YR may be an ideal albino tea germplasm for planting and breeding. Our results may help to characterize the leaf coloration and metabolic mechanism of albino tea germplasm.

scholarly journals Transcriptional profile of AvrRpt2EA-mediated resistance and susceptibility response to Erwinia amylovora in apple

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Susan Schröpfer ◽  
Isabelle Vogt ◽  
Giovanni Antonio Lodovico Broggini ◽  
Andreas Dahl ◽  
Klaus Richter ◽  
...  
Keyword(s):  
Heat Shock ◽  
Fire Blight ◽  
Erwinia Amylovora ◽  
Bacterial Disease ◽  
Resistance Response ◽  
Heat Shock Transcription Factors ◽  
Induce Resistance ◽  
Apple Cultivars ◽  
Shock Proteins ◽  
Susceptible Response

AbstractMost of the commercial apple cultivars are highly susceptible to fire blight, which is the most devastating bacterial disease affecting pome fruits. Resistance to fire blight is described especially in wild Malus accessions such as M. × robusta 5 (Mr5), but the molecular basis of host resistance response to the pathogen Erwinia amylovora is still largely unknown. The bacterial effector protein AvrRpt2EA was found to be the key determinant of resistance response in Mr5. A wild type E. amylovora strain and the corresponding avrRpt2EA deletion mutant were used for inoculation of Mr5 to induce resistance or susceptible response, respectively. By comparison of the transcriptome of both responses, 211 differentially expressed genes (DEGs) were identified. We found that heat-shock response including heat-shock proteins (HSPs) and heat-shock transcription factors (HSFs) are activated in apple specifically in the susceptible response, independent of AvrRpt2EA. Further analysis on the expression progress of 81 DEGs by high-throughput real-time qPCR resulted in the identification of genes that were activated after inoculation with E. amylovora. Hence, a potential role of these genes in the resistance to the pathogen is postulated, including genes coding for enzymes involved in formation of flavonoids and terpenoids, ribosome-inactivating enzymes (RIPs) and a squamosa promoter binding-like (SPL) transcription factor.

Activation of heat-shock transcription factor in rat heart after heat shock and exercise

1995 ◽  
Vol 268 (6) ◽  
pp. C1387-C1394 ◽  
Author(s):  
M. Locke ◽  
E. G. Noble ◽  
R. M. Tanguay ◽  
M. R. Feild ◽  
S. E. Ianuzzo ◽  
...  
Keyword(s):  
Heat Shock ◽  
Cultured Cells ◽  
Treadmill Running ◽  
Rat Myocardium ◽  
Sprague Dawley ◽  
Mobility Shift ◽  
Heat Shock Element ◽  
Heat Shock Transcription Factors ◽  
Sprague Dawley Rats ◽  
Shock Proteins

Stress-induced transcriptional regulation of the heat-shock proteins (HSP) is mediated by activation and binding of the heat-shock transcription factors (HSF) to the heat-shock element (HSE). Given the similarities between the stressors known to activate the HSF in cultured cells and the physiological stresses known to occur during exercise, HSF activation was examined in the hearts from exercising animals. Sprague-Dawley rats (5 rats/group) were run on a treadmill (24 m/min) for either 0, 20, 40, or 60 min or to exhaustion (102 +/- 7 min). Protein extracts were assessed for HSF activation by mobility-shift gels. Extracts from the hearts of nonrunning rats demonstrated no HSF activation, whereas HSF activation was detected in 80% of the hearts from animals that run for at least 40 min. These results demonstrate that treadmill running is capable of activating the HSF and increasing 70-kDa HSP mRNA in the rat myocardium.

scholarly journals Priming by High Temperature Stress Induces MicroRNA Regulated Heat Shock Modules Indicating Their Involvement in Thermopriming Response in Rice

Life ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 291
Author(s):  
Akhilesh Kumar Kushawaha ◽  
Ambreen Khan ◽  
Sudhir Kumar Sopory ◽  
Neeti Sanan-Mishra
Keyword(s):  
High Temperature ◽  
Heat Shock ◽  
Temperature Stress ◽  
High Throughput Sequencing ◽  
High Temperature Stress ◽  
Short Exposure ◽  
Heat Shock Transcription Factors ◽  
Survival And Development ◽  
Subsequent Exposure ◽  
Shock Proteins

Rice plants often encounter high temperature stress, but the associated coping strategies are poorly understood. It is known that a prior shorter exposure to high temperature, called thermo-priming, generally results in better adaptation of the plants to subsequent exposure to high temperature stress. High throughput sequencing of transcript and small RNA libraries of rice seedlings primed with short exposure to high temperature followed by high temperature stress and from plants exposed to high temperature without priming was performed. This identified a number of transcripts and microRNAs (miRs) that are induced or down regulated. Among them osa-miR531b, osa-miR5149, osa-miR168a-5p, osa-miR1846d-5p, osa-miR5077, osa-miR156b-3p, osa-miR167e-3p and their respective targets, coding for heat shock activators and repressors, showed differential expression between primed and non-primed plants. These findings were further validated by qRT-PCR. The results indicate that the miR-regulated heat shock proteins (HSPs)/heat shock transcription factors (HSFs) may serve as important regulatory nodes which are induced during thermo-priming for plant survival and development under high temperatures.

Heat shock proteins in willow (Salix viminalis)

1990 ◽  
Vol 80 (2) ◽  
pp. 301-306
Author(s):  
Tiina Vahala ◽  
Tage Eriksson ◽  
Peter Engstrom
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Salix Viminalis ◽  
Shock Proteins
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