scholarly journals Genetic Structure of Norway Spruce Ecotypes Studied by SSR Markers

Forests ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 110 ◽  
Author(s):  
Zuzana Bínová ◽  
Jiří Korecký ◽  
Jakub Dvořák ◽  
Jan Bílý ◽  
Dagmar Zádrapová ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Norway Spruce ◽  
Ssr Markers ◽  
Expressed Sequence Tag ◽  
Morphological Differentiation ◽  
Common Garden ◽  
High Elevation ◽  
Morphological Variants ◽  
Common Garden Experiments ◽  
Genetically Determined

Norway spruce is a widespread and economically highly important tree species in Central Europe which occurs there in different morphotypic forms (also known as ecotypes). Previously established common garden experiments indicated that the morphological differentiation is most likely genetically determined. The genetic structure of Norway spruce morphological variants might be an indicator (marker) of specific sustainability in forest ecosystems. In this study, we investigated 436 individuals from autochthonous populations belonging to three different ecotypes. The main aim was to evaluate a level of genetic intra and interpopulation diversity among the low, medium and high-elevation ecotypes using both expressed sequence tag simple sequence repeats (EST – SSR) and genomic SSR markers. Sixteen highly polymorphic microsatellite loci folded in two newly designed multiplexes were used to depicture the genetic structure of targeted trees. Important allele frequency parameters, such as the mean expected (0.722, SE = 0.061) and observed (0.585, SE = 0.062) heterozygosity and mean effective number of alleles (Ne = 5.943, SE = 1.279), were estimated. The low genetic differentiation detected among different ecotypes (Fst = 0.008) was further discussed and clarified.

Genetic structure of wild emmer wheat populations as reflected by transcribed versus anonymous SSR markers

Genome ◽  
2008 ◽  
Vol 51 (3) ◽  
pp. 187-195 ◽  
Author(s):  
Zvi Peleg ◽  
Tzion Fahima ◽  
Shahal Abbo ◽  
Tamar Krugman ◽  
Yehoshua Saranga
Keyword(s):  
Genetic Structure ◽  
Ssr Markers ◽  
Expressed Sequence Tag ◽  
Triticum Turgidum ◽  
Gene Diversity ◽  
Polymorphic Information Content ◽  
Allelic Diversity ◽  
Wild Emmer Wheat ◽  
Emmer Wheat ◽  
Wild Emmer

Simple sequence repeat (SSR) markers have become a major tool in population genetic analyses. The anonymous genomic SSRs (gSSRs) have been recently supplemented with expressed sequence tag (EST) derived SSRs (eSSRs), which represent the transcribed regions of the genome. In the present study, we used 8 populations of wild emmer wheat ( Triticum turgidum subsp. dicoccoides ) to compare the usefulness of the two types of SSR markers in assessing allelic diversity and population structure. gSSRs revealed significantly higher diversity than eSSRs in terms of average number of alleles (14.92 vs. 7.4, respectively), polymorphic information content (0.87 vs. 0.68, respectively), and gene diversity (He; 0.55 vs. 0.38, respectively). Despite the overall differences in the level of diversity, Mantel tests for correlations between eSSR and gSSR pairwise genetic distances were found to be significant for each population as well as for all accessions jointly (RM = 0.54, p = 0.01). Various genetic structure analyses (AMOVA, PCoA, STRUCTURE, unrooted UPGMA tree) revealed a better capacity of eSSRs to distinguish between populations, while gSSRs showed a higher proportion of intrapopulation (among accessions) diversity. We conclude that eSSR and gSSR markers should be employed in conjunction to obtain a high inter- and intra-specific (or inter- and intra-varietal) distinctness.

scholarly journals Genetic Variation Related to High Elevation Adaptation Revealed by Common Garden Experiments in Pinus yunnanensis

2020 ◽  
Vol 10 ◽  
Author(s):  
Yan-Qiang Sun ◽  
Wei Zhao ◽  
Chao-Qun Xu ◽  
Yulan Xu ◽  
Yousry A. El-Kassaby ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Common Garden ◽  
High Elevation ◽  
Pinus Yunnanensis ◽  
Common Garden Experiments

Experimental evidence for the biological species status in Lake Tana's Labeobarbus flock (Cyprinidae)

2010 ◽  
Vol 60 (2) ◽  
pp. 183-193 ◽  
Author(s):  
Arjan Palstra ◽  
Martin de Graaf ◽  
Leo Nagelkerke ◽  
Ferdinand Sibbing
Keyword(s):  
Genetic Basis ◽  
Direct Proof ◽  
Morphological Differentiation ◽  
Common Garden ◽  
Biological Species ◽  
Species Flock ◽  
Lake Tana ◽  
Cyprinid Fishes ◽  
Morphological Differences ◽  
Common Garden Experiments

AbstractLake Tana (Ethiopia) harbours the only known remaining intact species flock of large (max. 100 cm standard length, SL) cyprinid fishes (15 Labeobarbus spp.). In 'common garden' experiments progeny of the riverine spawning benthivorous L. tsanensis, and of the piscivorous L. truttiformis and L. megastoma was raised under similar environmental conditions to test if interspecific morphological differentiation would occur. Interspecific morphological differences and divergence were clearly observed early in ontogeny (≤ 40 mm SL). This study is the first to demonstrate direct proof for the genetic basis of morphological differentiation among these labeobarbs, providing further support that Lake Tana's labeobarb species are true biological species.

Sex ratio and life history traits at reaching sexual maturity in the dioecious shrub Fuchsia parviflora: field and common garden experiments

2021 ◽  
pp. 1-6
Author(s):  
Jessica S. Ambriz ◽  
Clementina González ◽  
Eduardo Cuevas
Keyword(s):  
Natural Population ◽  
Sexual Maturity ◽  
Sex Ratios ◽  
Natural Populations ◽  
Common Garden ◽  
Common Garden Experiment ◽  
Trade Offs ◽  
Reproductive Biomass ◽  
Common Garden Experiments ◽  
Growth And Reproduction

Abstract Fuchsia parviflora is a dioecious shrub that depends on biotic pollination for reproduction. Previous studies suggest that the male plants produce more flowers, and male-biased sex ratios have been found in some natural populations. To assess whether the biased sex ratios found between genders in natural populations are present at the point at which plants reach sexual maturity, and to identify possible trade-offs between growth and reproduction, we performed a common garden experiment. Finally, to complement the information of the common garden experiment, we estimated the reproductive biomass allocation between genders in one natural population. Sex ratios at reaching sexual maturity in F. parviflora did not differ from 0.5, except in one population, which was the smallest seedling population. We found no differences between genders in terms of the probability of germination or flowering. When flowering began, female plants were taller than males and the tallest plants of both genders required more time to reach sexual maturity. Males produced significantly more flowers than females, and the number of flowers increased with plant height in both genders. Finally, in the natural population studied, the investment in reproductive biomass was seven-fold greater in female plants than in male plants. Our results showed no evidence of possible trade-offs between growth and reproduction. Despite the fact that female plants invest more in reproductive biomass, they were taller than the males after flowering, possibly at the expense of herbivory defence.

scholarly journals Characterization of the Genetic Diversity Present in a Diverse Sesame Landrace Collection Based on Phenotypic Traits and EST-SSR Markers Coupled With an HRM Analysis

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 656
Author(s):  
Evangelia Stavridou ◽  
Georgios Lagiotis ◽  
Parthena Kalaitzidou ◽  
Ioannis Grigoriadis ◽  
Irini Bosmali ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Geographical Origin ◽  
Expressed Sequence Tag ◽  
Phenotypic Diversity ◽  
Molecular Data ◽  
Phenotypic Traits ◽  
Dissimilarity Matrix ◽  
Heterotic Groups ◽  
Hrm Analysis ◽  
Sesame Genotypes

A selection of sesame (Sesamum indicum L.) landraces of different eco-geographical origin and breeding history have been characterized using 28 qualitative morpho-physiological descriptors and seven expressed sequence tag-simple sequence repeat (EST-SSR) markers coupled with a high-resolution melting (HRM) analysis. The most variable qualitative traits that could efficiently discriminate landraces, as revealed by the correlation analyses, were the plant growth type and position of the branches, leaf blade width, stem pubescence, flowering initiation, capsule traits and seed coat texture. The agglomerative hierarchical clustering analysis based on a dissimilarity matrix highlighted three main groups among the sesame landraces. An EST-SSR marker analysis revealed an average polymorphism information content (PIC) value of 0.82, which indicated that the selected markers were highly polymorphic. A principal coordinate analysis and dendrogram reconstruction based on the molecular data classified the sesame genotypes into four major clades. Both the morpho-physiological and molecular analyses showed that landraces from the same geographical origin were not always grouped in the same cluster, forming heterotic groups; however, clustering patterns were observed for the Greek landraces. The selective breeding of such traits could be employed to unlock the bottleneck of local phenotypic diversity and create new cultivars with desirable traits.

Adaptation of Mediterranean forest species to climate: lessons from common garden experiments

2021 ◽  
Author(s):  
JA Ramírez‐Valiente ◽  
L Santos del Blanco ◽  
R Alía ◽  
JJ Robledo‐Arnuncio ◽  
J Climent
Keyword(s):  
Common Garden ◽  
Mediterranean Forest ◽  
Forest Species ◽  
Common Garden Experiments

Genetic structure of cultivated Zanthoxylum species investigated with SSR markers

2018 ◽  
Vol 14 (6) ◽  
Author(s):  
Yang Hu ◽  
Lu Tian ◽  
Jingwei Shi ◽  
Jieyun Tian ◽  
Lili Zhao ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Ssr Markers

Genetic structure of Iranian olive cultivars and their relationship with Mediterranean's cultivars revealed by SSR markers

2014 ◽  
Vol 178 ◽  
pp. 175-183 ◽  
Author(s):  
Zahra Noormohammadi ◽  
Isabel Trujillo ◽  
Angjelina Belaj ◽  
Saeede Ataei ◽  
Mehdi Hosseini-Mazinan
Keyword(s):  
Genetic Structure ◽  
Ssr Markers ◽  
Olive Cultivars
Sign in / Sign up

Export Citation Format

Share Document