scholarly journals Phytophthora Species from Xinjiang Wild Apple Forests in China

Forests ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 927
Author(s):  
Xu ◽  
Huai ◽  
Hamiti ◽  
Zhang ◽  
Zhao
Keyword(s):  
Internal Transcribed Spacer ◽  
Phytophthora Species ◽  
Soil Samples ◽  
Tianshan Mountain ◽  
Natural Ecosystems ◽  
Malus Sieversii ◽  
Germplasm Resource ◽  
Its Sequence Analysis ◽  
Canopy Drip ◽  
Comprehensive Study

Phytophthora species are well-known destructive forest pathogens, especially in natural ecosystems. The wild apple (Malus sieversii (Ledeb.) Roem.) is the primary ancestor of M. domestica (Borkh.) and important germplasm resource for apple breeding and improvement. During the period from 2016 to 2018, a survey of Phytophthora diversity was performed at four wild apple forest plots (Xin Yuan (XY), Ba Lian (BL), Ku Erdening (KE), and Jin Qikesai (JQ)) on the northern slopes of Tianshan Mountain in Xinjiang, China. Phytophthora species were isolated from baiting leaves from stream, canopy drip, and soil samples and were identified based on morphological observations and the rDNA internal transcribed spacer (ITS) sequence analysis. This is the first comprehensive study from Xinjiang to examine the Phytophthora communities in wild apple forests The 621 resulting Phytophthora isolates were found to reside in 10 different Phytophthora species: eight known species (P. lacustris being the most frequent, followed by P. gonapodyides, P. plurivora, P. gregata, P. chlamydospora, P. inundata, P. virginiana, and P. cactorum) and two previously unrecognized species (P. sp. CYP74 and P. sp. forestsoil-like). The highest species richness of Phytophthora occurred at BL, followed by XY. P. lacustris was the dominant species at BL, XY, and JQ, while P. gonapodyides was the most common at KE. In the present paper, the possible reasons for their distribution, associated implications, and associated diseases are discussed.

scholarly journals WildTermitomycesSpecies Collected from Ondo and Ekiti States Are More Related to African Species as Revealed by ITS Region of rDNA

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Victor Olusegun Oyetayo
Keyword(s):  
Sequence Analysis ◽  
Phylogenetic Tree ◽  
Molecular Identification ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Its Sequence ◽  
African Countries ◽  
African Species ◽  
Its Sequence Analysis ◽  
Degree Of Similarity

Molecular identification of eighteenTermitomycesspecies collected from two states, Ondo and Ekiti in Nigeria was carried out using the internal transcribed spacer (ITS) region. The amplicons obtained from rDNA ofTermitomycesspecies were compared with existing sequences in the NCBI GenBank. The results of the ITS sequence analysis discriminated between all theTermitomycesspecies (obtained from Ondo and Ekiti States) andTermitomycessp. sequences obtained from NCBI GenBank. The degree of similarity of T1 to T18 to gene ofTermitomycessp. obtained from NCBI ranges between 82 and 99 percent.Termitomycesspecies from Garbon with ascension number AF321374 was the closest relative of T1 to T18 except T12 that has T. eurhizus and T. striatus as the closet relative. Phylogenetic tree generated with ITS sequences obtained from NCBI GenBank data revealed that T1 to T18 are more related toTermitomycesspecies indigenous to African countries such as Senegal, Congo, and Gabon.

scholarly journals Ecosystems of protected area of the Natural Park “Medeu”

2021 ◽  
Vol 20 (1) ◽  
pp. 169-173
Author(s):  
L. A. Dimeyeva ◽  
V. N. Permitina ◽  
R. E. Sadvokasov ◽  
B. Sh. Kaliyev ◽  
Zh. K. Salmukhanbetova
Keyword(s):  
Protected Area ◽  
Buffer Zone ◽  
Mountain Range ◽  
Natural Ecosystems ◽  
Red Data Book ◽  
Malus Sieversii ◽  
Natural Park ◽  
Data Book ◽  
Celtis Australis ◽  
Anthropogenic Ecosystems

The article presents the results of mapping ecosystems of the protected area of the Natural Park “Medeu”,located on the northern slope of the Trans-Ili Alatau mountain range. The map of ecosystems and a legend has beencompiled. The legend contains 13 numbers. The largest sections of the legend are the natural ecosystems of the protectedarea and the natural-anthropogenic ecosystems of the buffer zone and the zone of limited economic activity. The habitatsof species from Red Data book of Kazakhstan that form communities (Malus sieversii (Ledeb.) M. Roem., Armeniacavulgaris Lam., Celtis australis subsp. caucasica (Willd.) C. C. Towns. identified, and their brief characteristics are given.

scholarly journals A new species of Acrophialophora from Guizhou Province, China

Phytotaxa ◽  
2017 ◽  
Vol 302 (3) ◽  
pp. 266
Author(s):  
YANWEI ZHANG ◽  
YAO WANG ◽  
GUI-PING ZENG ◽  
WANHAO CHEN ◽  
ZOU XIAO ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Distinct Species ◽  
Soil Samples ◽  
Guizhou Province ◽  
Beijing City ◽  
Living Culture ◽  
A New Species ◽  
Β Tubulin

Acrophialophora liboensis, a new fungus from soil samples in Libo County, Guizhou Province, China, is illustrated and described on the basis of morphological and molecular sequences data. Phylogenetic analysis based on internal transcribed spacer (ITS) and β-tubulin sequences demonstrated that A. liboensis is a distinct species closely related to A. cinerea and A. furcata. Morphologically, A. liboensis is characterized by solitary and lateral phialides tapering into thin necks and long chains of ellipsoidal or oval conidia. The ex-type living culture has been deposited in CGMCC, Beijing City, China.

scholarly journals The Genus Phytophthora Anno 2012

2012 ◽  
Vol 102 (4) ◽  
pp. 348-364 ◽  
Author(s):  
Laurens P. N. M. Kroon ◽  
Henk Brouwer ◽  
Arthur W. A. M. de Cock ◽  
Francine Govers
Keyword(s):  
New Species ◽  
Late Blight ◽  
19Th Century ◽  
Phytophthora Species ◽  
Plant Diseases ◽  
Natural Ecosystems ◽  
Monthly Basis ◽  
Irish Famine ◽  
Great Irish Famine ◽  
The 19Th Century

Plant diseases caused by Phytophthora species will remain an ever increasing threat to agriculture and natural ecosystems. Phytophthora literally means plant destroyer, a name coined in the 19th century by Anton de Bary when he investigated the potato disease that set the stage for the Great Irish Famine. Phytophthora infestans, the causal agent of potato late blight, was the first species in a genus that at present has over 100 recognized members. In the last decade, the number of recognized Phytophthora species has nearly doubled and new species are added almost on a monthly basis. Here we present an overview of the 10 clades that are currently distinguished within the genus Phytophthora with special emphasis on new species that have been described since 1996 when Erwin and Ribeiro published the valuable monograph ‘Phytophthora diseases worldwide’ (35).

Comparison of the ITS Sequences of 5 Common Potentilla Species in Jilin Province of China

2012 ◽  
Vol 554-556 ◽  
pp. 1690-1693 ◽  
Author(s):  
Shao Xuan Zhang ◽  
Xin Rui Liu ◽  
Bo Chuan Wang ◽  
Yun Hui Ling ◽  
De Jun Sun ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Its Region ◽  
Scientific Data ◽  
Jilin Province ◽  
Its Sequences ◽  
Universal Primers ◽  
Its Sequence ◽  
Its Sequence Analysis ◽  
Pcr Products

To find the differences in the internal transcribed spacer(ITS) sequences and provide scientific data for the authentication of Potentilla chinensis and its related species, we extracted the genome DNA from the leaves of 5 common Potetilla species in Jilin Province, amplified the ITS region using ITS universal primers of angiosperm, and sequenced the purified PCR products directly. Polymorphism of ITS sequences was found within P. chinensis and the sequence data suggested that our samples of this species might be related to hybridization. Other 4 species showed intraspecies-stability in ITS sequence. The ITS sequences of these 5 Potentilla species are significantly different. So ITS sequence analysis and other methods derived from it can be used in authentication of Potentilla.

scholarly journals Perennial Existence of Organochlorine Pesticides in the Soils of Amghara, Kuwait

2021 ◽  
Author(s):  
Hassan Alshemmari
Keyword(s):  
Distribution Pattern ◽  
Organochlorine Pesticides ◽  
Total Concentration ◽  
Residue Analysis ◽  
Soil Samples ◽  
Average Value ◽  
Ionization Mode ◽  
High Degree ◽  
The Impact ◽  
Comprehensive Study

Abstract A comprehensive study from the surface soil samples of 14 locations from Amghara, Kuwait were assessed for the investigation organochlorine pesticides (OCPs). Due the high degree of contamination in the environment and the health consequences of OCPs, the assessment of these compounds had a significant concern. There is limited informations regarding the distribution pattern of OCPs in the soil samples of Kuwait. The study comprises 12 OCPs including their isomers. Pesticide residue analysis was done with a gas chromatograph for organochlorine pesticides (OCPs) coupled to a triple quadruple mass spectrometer in electron ionization mode. The total concentration of OCPs were in the range of 209.39 pg/g -7449.18 pg/g with an average value of 1313.04 pg/g. DDT had higher concentrations in soil samples (969.52 pg/g) than the other pesticides, according to the findings. The distribution pattern of OCPs in the Amghara soils revealed their origin as both historical and recent application of pesticides. The impact of soil pH on the distribution of DDTs in Amghara soil samples were also investigated. The study further looked at how residual quantities could be used to determine health risks of both children and adults. Children and adults in all the locations were subject to negligible cancer risk, according to the health risk evaluation. OCP's cancer dangers from ingestion, dermal exposure, and inhalation of soil particles indicated that all stations were in the safe zone.

scholarly journals Variability in phosphite sensitivity observed within and between seven Phytophthora species

2022 ◽  
Author(s):  
Shannon Hunter ◽  
Rebecca McDougal ◽  
Nari Williams ◽  
Peter Scott
Keyword(s):  
Mycelial Growth ◽  
Prolonged Exposure ◽  
Intraspecific Variability ◽  
Phytophthora Species ◽  
Growth Experiment ◽  
Natural Ecosystems ◽  
Defence Responses ◽  
Needle Blight

AbstractPhosphite is used to control and manage many phytophthora diseases in horticultural systems worldwide and natural ecosystems in Australia, Africa, New Zealand and parts of Northern America and Europe. Phosphite does not kill Phytophthora species, but inhibits growth while also stimulating host defence responses. Phytophthora species differ in their underlying tolerance to phosphite and isolates have been shown to acquire tolerance after prolonged exposure. Intra- and inter-specific variability in phosphite sensitivity is of interest to determine the efficacy and sustainability of phosphite for the treatment of phytophthora diseases, which continue to spread globally. Seven Phytophthora species were tested for their sensitivity to phosphite in vitro in a mycelial growth experiment. Phytophthora agathidicida was the species most sensitive to phosphite, being inhibited by 98.7% on average at the lowest phosphite treatment (15 µg/mL phosphite), followed by P. aleatoria, P. cinnamomi, P. pluvialis, P. multivora, P. kernoviae and P. citricola. Huge intraspecific variability was observed with P. kernoviae, which raises the question of whether diseases caused by P. kernoviae such as phytophthora needle blight of Pinus radiata could be managed effectively with phosphite. Further work is required to determine the phosphite sensitivity of different introduced and native Phytophthora species growing in key hosts and whether tolerance observed in vitro is also expressed in vivo.

scholarly journals An overview of Australia’s Phytophthora species assemblage in natural ecosystems recovered from a survey in Victoria

IMA Fungus ◽  
2016 ◽  
Vol 7 (1) ◽  
pp. 47-58 ◽  
Author(s):  
William A. Dunstan ◽  
Kay Howard ◽  
Giles E. StJ. Hardy ◽  
Treena I. Burgess
Keyword(s):  
Phytophthora Species ◽  
Species Assemblage ◽  
Natural Ecosystems
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