scholarly journals Production of Ethanol from Livestock, Agricultural, and Forest Residuals: An Economic Feasibility Study

Environments ◽  
2019 ◽  
Vol 6 (8) ◽  
pp. 97 ◽  
Author(s):  
Kyoung S Ro ◽  
Mark A Dietenberger ◽  
Judy A Libra ◽  
Richard Proeschel ◽  
Hasan K. Atiyeh ◽  
...  
Keyword(s):  
Cost Analysis ◽  
Wheat Straw ◽  
Corn Stover ◽  
Regional Scale ◽  
Scale Up ◽  
Swine Manure ◽  
Economic Feasibility ◽  
Gasoline Prices ◽  
Construction Costs ◽  
Facility Size

In this study, the economic feasibility of producing ethanol from gasification followed by syngas fermentation via commercially available technologies was theoretically evaluated using a set of selected livestock and agricultural and forest residuals ranging from low valued feedstocks (i.e., wood, wheat straw, wheat straws blended with dewatered swine manure, and corn stover) to high valued oilseed rape meal. A preliminary cost analysis of an integrated commercial system was made for two cases, a regional scale 50 million gallon (189,271 m3) per year facility (MGY) and a co-op scale 1–2 MGY facility. The estimates for the minimum ethanol selling prices (MESP) depend heavily on the facility size and feedstock costs. For the 1–2 MGY (3785–7571 m3/y) facility, the MESP ranged from $5.61–$7.39 per gallon ($1.48–$1.95 per liter) for the four low-value feedstocks. These high costs suggest that the co-op scale even for the low-value feedstocks may not be economically sustainable. However, the MESP for the 50 MGY facility were significantly lower and comparable to gasoline prices ($2.24–$2.96 per gallon or $0.59–$0.78 per liter) for these low-value feedstocks, clearly showing the benefits of scale-up on construction costs and MESP.

scholarly journals Microdiversity and phylogeographic diversification of bacterioplankton in pelagic freshwater systems revealed through long-read amplicon sequencing

Microbiome ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Yusuke Okazaki ◽  
Shohei Fujinaga ◽  
Michaela M. Salcher ◽  
Cristiana Callieri ◽  
Atsushi Tanaka ◽  
...  
Keyword(s):  
16S Rrna ◽  
Regional Scale ◽  
Scale Up ◽  
Amplicon Sequencing ◽  
Freshwater Ecosystems ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Metagenomic Sequencing ◽  
Long Read

Abstract Background Freshwater ecosystems are inhabited by members of cosmopolitan bacterioplankton lineages despite the disconnected nature of these habitats. The lineages are delineated based on > 97% 16S rRNA gene sequence similarity, but their intra-lineage microdiversity and phylogeography, which are key to understanding the eco-evolutional processes behind their ubiquity, remain unresolved. Here, we applied long-read amplicon sequencing targeting nearly full-length 16S rRNA genes and the adjacent ribosomal internal transcribed spacer sequences to reveal the intra-lineage diversities of pelagic bacterioplankton assemblages in 11 deep freshwater lakes in Japan and Europe. Results Our single nucleotide-resolved analysis, which was validated using shotgun metagenomic sequencing, uncovered 7–101 amplicon sequence variants for each of the 11 predominant bacterial lineages and demonstrated sympatric, allopatric, and temporal microdiversities that could not be resolved through conventional approaches. Clusters of samples with similar intra-lineage population compositions were identified, which consistently supported genetic isolation between Japan and Europe. At a regional scale (up to hundreds of kilometers), dispersal between lakes was unlikely to be a limiting factor, and environmental factors or genetic drift were potential determinants of population composition. The extent of microdiversification varied among lineages, suggesting that highly diversified lineages (e.g., Iluma-A2 and acI-A1) achieve their ubiquity by containing a consortium of genotypes specific to each habitat, while less diversified lineages (e.g., CL500-11) may be ubiquitous due to a small number of widespread genotypes. The lowest extent of intra-lineage diversification was observed among the dominant hypolimnion-specific lineage (CL500-11), suggesting that their dispersal among lakes is not limited despite the hypolimnion being a more isolated habitat than the epilimnion. Conclusions Our novel approach complemented the limited resolution of short-read amplicon sequencing and limited sensitivity of the metagenome assembly-based approach, and highlighted the complex ecological processes underlying the ubiquity of freshwater bacterioplankton lineages. To fully exploit the performance of the method, its relatively low read throughput is the major bottleneck to be overcome in the future.

Scale-up and cost analysis of a photo-Fenton system for sanitary landfill leachate treatment

2016 ◽  
Vol 283 ◽  
pp. 76-88 ◽  
Author(s):  
Tânia F.C.V. Silva ◽  
Amélia Fonseca ◽  
Isabel Saraiva ◽  
Rui A.R. Boaventura ◽  
Vítor J.P. Vilar
Keyword(s):  
Cost Analysis ◽  
Landfill Leachate ◽  
Scale Up ◽  
Sanitary Landfill ◽  
Leachate Treatment ◽  
Fenton System ◽  
Sanitary Landfill Leachate

scholarly journals Lignocellulose pretreatment technologies affect the level of enzymatic cellulose oxidation by LPMO

2015 ◽  
Vol 17 (5) ◽  
pp. 2896-2903 ◽  
Author(s):  
Ursula Fabiola Rodríguez-Zúñiga ◽  
David Cannella ◽  
Roberto de Campos Giordano ◽  
Raquel de Lima Camargo Giordano ◽  
Henning Jørgensen ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Corn Stover ◽  
Sugarcane Bagasse ◽  
Cellulosic Ethanol ◽  
Cellulose Oxidation ◽  
Available Resources

Sugarcane bagasse, corn stover, and wheat straw are among the most available resources for the production of cellulosic ethanol.

Production of Methane Gas From Combinations of Wheat Straw and Swine Manure

1983 ◽  
Vol 26 (2) ◽  
pp. 0546-0548 ◽  
Author(s):  
J. R. Fischer ◽  
E. L. Iannotti ◽  
C. D. Fulhage
Keyword(s):  
Wheat Straw ◽  
Swine Manure ◽  
Methane Gas

scholarly journals TERENO: German network of terrestrial environmental observatories

2016 ◽  
Vol 2 ◽  
Author(s):  
Heye Reemt Bogena
Keyword(s):  
Regional Scale ◽  
Scale Up ◽  
Climate Change Impacts ◽  
Integrated Modelling ◽  
Small Scale ◽  
Catchment Scale ◽  
Terrestrial Environment ◽  
Regional Analyses ◽  
Research Facilities

Central elements of the TERENO network are “terrestrial observatories” at the catchment scale which were selected in climate sensitive regions of Germany for the regional analyses of climate change impacts. Within these observatories small scale research facilities and test areas are placed in order to accomplish energy, water, carbon and nutrient process studies across the different compartments of the terrestrial environment. Following a hierarchical scaling approach (point-plot-field) these detailed information and the gained knowledge will be transferred to the regional scale using integrated modelling approaches. Furthermore, existing research stations are enhanced and embedded within the observatories. In addition, mobile measurement platforms enable monitoring of dynamic processes at the local scale up to the determination of spatial pattern at the regional scale are applied within TERENO.

Expression of serpins by Clostridium thermocellum and simultaneous saccharification of lignocellulosic biomass to enhance ethanol production

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 8662-8676
Author(s):  
Maria Mushtaq ◽  
Muhammad Javaid Asad ◽  
Muhammad Zeeshan Hyder ◽  
Syed Muhammad Saqlan Naqvi ◽  
Saad Imran Malik ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Wheat Straw ◽  
Corn Stover ◽  
Rice Straw ◽  
Clostridium Thermocellum ◽  
Ethanol Yield ◽  
Biofuel Production ◽  
Yeast Fermentation ◽  
Second Generation Bioethanol ◽  
Simultaneous Saccharification

Utilization of biomass for production of second generation bioethanol was considered as a way to reduce burdens of fossil fuel in Pakistan. The materials wheat straw, rice straw, cotton stalk, corn stover, and peel wastes were used in this experiment. Various parameters, such as acidic and alkali pretreatment, enzymatic hydrolysis by cellulases, and effect of proteases inhibitors on ethanol production, were examined. Fermentation was completed by the yeasts Saccharomyces cerevisiae and Clostridium thermocellum separately, and their ethanol production were compared and maximum ethanol yield was obtained with wheat straw i.e.,11.3 g/L by S. cerevisiae and 8.5 g/L by C. thermocellum. Results indicated that a higher quantity of sugar was obtained from wheat straw (19.6 ± 1.6 g/L) followed by rice straw (17.6 ± 0.6 g/L) and corn stover (16.1 ± 0.9 g/L) compared to the other evaluated biomass samples. A higher yield of ethanol (11.3 g/L) was observed when a glucose concentration of 21.7 g/L was used, for which yeast fermentation efficiency was 92%. Results also revealed the increased in ethanol production (93%) by using celluases in combination with recombinant Serine protease inhibitors from C. thermocellum. It is expected that the use of recombinant serpins with cellulases will play a major role in the biofuel production by using agricultural biomass. This will also help in the economics of the biofuel.

scholarly journals Techno-Economic Bottlenecks of the Fungal Pretreatment of Lignocellulosic Biomass

Fermentation ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 30 ◽  
Author(s):  
Juliana Vasco-Correa ◽  
Ajay Shah
Keyword(s):  
Corn Stover ◽  
Production Cost ◽  
Low Cost ◽  
Economic Feasibility ◽  
Perennial Grasses ◽  
Process Improvements ◽  
Current State ◽  
Lignocellulosic Feedstocks ◽  
Fungal Pretreatment ◽  
Wastewater Generation

Fungal pretreatment is a biological process that uses rotting fungi to reduce the recalcitrance and enhance the enzymatic digestibility of lignocellulosic feedstocks at low temperature, without added chemicals and wastewater generation. Thus, it has been presumed to be low cost. However, fungal pretreatment requires longer incubation times and generates lower yields than traditional pretreatments. Thus, this study assesses the techno-economic feasibility of a fungal pretreatment facility for the production of fermentable sugars for a 75,700 m3 (20 million gallons) per year cellulosic bioethanol plant. Four feedstocks were evaluated: perennial grasses, corn stover, agricultural residues other than corn stover, and hardwood. The lowest estimated sugars production cost ($1.6/kg) was obtained from corn stover, and was 4–15 times as much as previous estimates for conventional pretreatment technologies. The facility-related cost was the major contributor (46–51%) to the sugar production cost, mainly because of the requirement of large equipment in high quantities, due to process bottlenecks such as low sugar yields, low feedstock bulk density, long fungal pretreatment times, and sterilization requirements. At the current state of the technology, fungal pretreatment at biorefinery scale does not appear to be economically feasible, and considerable process improvements are still required to achieve product cost targets.

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