scholarly journals Optimization of Cattle Manure and Food Waste Co-Digestion for Biohydrogen Production in a Mesophilic Semi-Continuous Process

Energies ◽  
2020 ◽  
Vol 13 (15) ◽  
pp. 3848
Author(s):  
Shuang Liu ◽  
Wenzhe Li ◽  
Guoxiang Zheng ◽  
Haiyan Yang ◽  
Longhai Li
Keyword(s):  
Hydrogen Production ◽  
Food Waste ◽  
Substrate Concentration ◽  
Continuous Process ◽  
Cattle Manure ◽  
Biohydrogen Production ◽  
Interactive Effects ◽  
Hydrogen Yield ◽  
Mixing Ratio ◽  
Organic Solid

Biohydrogen production from organic solid waste has shown particular advantages over other methods owing to the combination of waste reduction and bioenergy production. In this study, biohydrogen production from the co-digestion of cattle manure and food waste was optimized in a mesophilic semi-continuous process. To maximize hydrogen production, the effects of the mixing ratio (the proportion of food waste in the substrate), substrate concentration, and hydraulic retention time (HRT) on the co-digestion were systematically analyzed using a Box–Behnken design. The results showed that strong interactive effects existed between the three factors, and they had a direct effect on the responses. Hydrogen was primarily produced via the butyrate pathway, which was accompanied by the competing heterolactic fermentation pathway. Propionate and valerate produced from lipids and proteins, respectively, were obtained along with butyrate. The optimal process parameters included a mixing ratio of 47% to 51%, a substrate concentration of 76 to 86 g L−1, and an HRT of 2 d. Under these optimal conditions, the hydrogen production rate and hydrogen yield were higher than 1.00 L L−1 d−1 and 30.00 mL g−1 VS, respectively, and the predicted results were consistent with the experimental data. The results indicate that the co-digestion of cattle manure and food waste is a practical and economically promising approach for biohydrogen production.

scholarly journals Statistical Optimization of Biohydrogen Production from Palm Oil Mill Effluent by Natural Microflora

2009 ◽  
Vol 3 (1) ◽  
pp. 79-86 ◽  
Author(s):  
Zatilfarihiah Rasdi ◽  
Nor Aini Abdul Rahman ◽  
Suraini Abd-Aziz ◽  
Phang Lai-Yee ◽  
Mohd Zulkhairi ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Palm Oil ◽  
Substrate Concentration ◽  
Oxygen Demand ◽  
Biohydrogen Production ◽  
Palm Oil Mill Effluent ◽  
Optimum Conditions ◽  
Heat Treated ◽  
Natural Microflora ◽  
Palm Oil Mill

In this study, palm oil mill effluent (POME) was used as the substrate for biohydrogen production. Heat-treated POME sludge acclimated with POME incubated at 37°C for 24 hours was used as seed culture. Preliminary screening on the effects of inocula sizes, heat treatment, substrate concentration and pH of incubation by using a factorial design (FD) were conducted under mesophilic condition (37°C) using a serum vial (160 mL). The experimental results from two-level FD showed that pH and Chemical Oxygen Demand (COD) of POME significantly affected biohydrogen production. Optimizations of the specific hydrogen production (Ps) and the hydrogen production rate (Rm) were achieved by using a central composite design (CCD). The maximum Ps of 272 mL H2/g carbohydrate was obtained under optimum conditions of pH 5.75 and substrate concentration of 80 g/L. The maximum Rm of 98 mL H2/h was calculated under the optimum conditions of pH 5.98 and substrate concentration of 80 g/L. The optimized conditions obtained were subjected to a confirmation run and it showed reproducible data with a Ps of 226 mL H2/g carbohydrate and Rm of 72 mL H2/h.

Enhanced biohydrogen production from cotton stalk hydrolysate of Enterobacter cloacae WL1318 by overexpression of the formate hydrogen lyase activator gene

2020 ◽  
Author(s):  
Qin Zhang ◽  
Shaolin You ◽  
Yanbin Li ◽  
Xiaowei Qu ◽  
Hui Jiang
Keyword(s):  
Hydrogen Production ◽  
Recombinant Strain ◽  
Wild Strain ◽  
Enterobacter Cloacae ◽  
Biohydrogen Production ◽  
Hydrogen Yield ◽  
Cotton Stalk ◽  
Formate Hydrogen Lyase ◽  
High Efficient ◽  
Cotton Stalk Hydrolysate

Abstract Background: Biohydrogen production from lignocellulose has become an important hydrogen production method due to its diversity, renewability, and cheapness. Overexpression of the formate hydrogen lyase activator (fhlA) gene is a promising tactic for enhancement of hydrogen production in facultative anaerobic Enterobacter. As a species of Enterobacter, Enterobacter cloacae was reported as high efficient hydrogen-producing bacterium. However, little work has been reported in terms of cloning and expressing the fhlA gene in E. cloacae for lignocellulose-based hydrogen production.Results: In this study, the formate hydrogen lyase activator (fhlA) gene was cloned and overexpressed in Enterobacter cloacae WL1318. We found that the recombinant strain significantly enhanced cumulative hydrogen production by 188% following fermentation of cotton stalk hydrolysate for 24 h, and maintained improved production above 30% throughout the fermentation process compared to the wild strain. Accordingly, overexpression of the fhlA gene resulted in an enhanced hydrogen production potential (P) and maximum hydrogen production rate (Rm), as well as a shortened lag phase time (λ) for the recombinant strain. Additionally, the recombinant strain also displayed improved glucose (12%) and xylose (3.4%) consumption and hydrogen yield Y(H2/S) (37.0%) compared to the wild strain. Moreover, the metabolites and specific enzyme profiles demonstrated that reduced flux in the competitive branch, including succinic, acetic, and lactic acids, and ethanol generation, coupled with increased flux in the pyruvate node and formate splitting branch, benefited hydrogen synthesis. Conclusions: The results conclusively prove that overexpression of fhlA gene in E. cloacae WL1318 can effectively enhance the hydrogen production from cotton stalk hydrolysate, and reduce the metabolic flux in the competitive branch. It’s the first attempt to engineer the fhlA gene in the hydrogen producing bacterium E. cloacae. This work provides a highly efficient engineered bacterium for biohydrogen production from fermentation of lignocellulosic hydrolysate in the future.

scholarly journals Effect of Different Inoculum Combination on Biohydrogen Production from Melon Fruit Waste

2018 ◽  
Vol 7 (2) ◽  
pp. 101-109 ◽  
Author(s):  
Yumechris Amekan ◽  
Dyah Sekar A P Wangi ◽  
Muhammad Nur Cahyanto ◽  
Sarto Sarto ◽  
Jaka Widada
Keyword(s):  
Hydrogen Production ◽  
Operating Conditions ◽  
Production Performance ◽  
Biohydrogen Production ◽  
Cow Dung ◽  
Strictly Anaerobic ◽  
Hydrogen Yield ◽  
Production Type ◽  
Fruit Waste ◽  
Melon Fruit

The natural microbial consortium from many sources widely used for hydrogen production. Type of substrate and operating conditions applied on the biodigesters of the natural consortium used as inoculum impact the variation of species and number of microbes that induce biogas formation, so this study examined the effect of different inoculum source and its combination of biohydrogen production performance. The hydrogen producing bacteria from fruit waste digester (FW), cow dung digester (CD), and tofu waste digester (TW) enriched under strictly anaerobic conditions at 37OC. Inoculums from 3 different digesters (FW, CD, and TW) and its combination (FW-CD, CD-TW, FW-TW, and FW-CD-TW) were used to test the hydrogen production from melon waste with volatile solids (VS) concentration of 9.65 g/L, 37°C and initial pH 7.05 ± 0.05. The results showed that individual and combined inoculum produced the gas comprising hydrogen and carbon dioxide without any detectable methane. The highest cumulative hydrogen production of 743 mL (yield 207.56 mL/gVS) and 1,132 mL (yield 231.02 mL/gVS) was shown by FW and FW-CD-TW, respectively. Butyric, acetate, formic and propionic were the primary soluble metabolites produced by all the cultures, and the result proves that higher production of propionic acid can decrease hydrogen yield. Clostridium perfringens and Clostridium baratii prominently seen in all single and combination inoculum. Experimental evidence suggests that the inoculum from different biodigesters able to adapt well to the environmental conditions and the new substrate after a combination process as a result of metabolic flexibility derived from the microbial diversity in the community to produce hydrogen. Therefore, inoculum combination could be used as a strategy to improve systems for on-farm energy recovery from animal and plant waste to processing of food and municipal waste.Article History: Received February 5th 2018; Received in revised form May 7th 2018; Accepted June 2nd 2018; Available onlineHow to Cite This Article: Amekan, Y., Wangi, D.S.A.P., Cahyanto, M.N., Sarto and Widada, J. (2018) Effect of Different Inoculum Combination on Biohydrogen Production from Melon Fruit Waste. Int. Journal of Renewable Energy Development, 7(2), 101-109.https://doi.org/10.14710/ijred.7.2.101-10

scholarly journals Understanding the NaCl-dependent behavior of hydrogen production of a marine bacterium,Vibrio tritonius

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6769
Author(s):  
Nurhidayu Al-saari ◽  
Eri Amada ◽  
Yuta Matsumura ◽  
Mami Tanaka ◽  
Sayaka Mino ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Clean Energy ◽  
Biohydrogen Production ◽  
Hydrogen Yield ◽  
Substrate Consumption ◽  
Molar Yield ◽  
Fermentative Hydrogen Production ◽  
Dependent Behavior ◽  
Wide Range ◽  
Fermentative Hydrogen

Biohydrogen is one of the most suitable clean energy sources for sustaining a fossil fuel independent society. The use of both land and ocean bioresources as feedstocks show great potential in maximizing biohydrogen production, but sodium ion is one of the main obstacles in efficient bacterial biohydrogen production.Vibrio tritoniusstrain AM2 can perform efficient hydrogen production with a molar yield of 1.7 mol H2/mol mannitol, which corresponds to 85% theoretical molar yield of H2production, under saline conditions. With a view to maximizing the hydrogen production using marine biomass, it is important to accumulate knowledge on the effects of salts on the hydrogen production kinetics. Here, we show the kinetics in batch hydrogen production ofV. tritoniusstrain AM2 to investigate the response to various NaCl concentrations. The modified Han–Levenspiel model reveals that salt inhibition in hydrogen production usingV. tritoniusstarts precisely at the point where 10.2 g/L of NaCl is added, and is critically inhibited at 46 g/L. NaCl concentration greatly affects the substrate consumption which in turn affects both growth and hydrogen production. The NaCl-dependent behavior of fermentative hydrogen production ofV. tritoniuscompared to that ofEscherichia coliJCM 1649 reveals the marine-adapted fermentative hydrogen production system inV. tritonius.V. tritoniusAM2 is capable of producing hydrogen from seaweed carbohydrate under a wide range of NaCl concentrations (5 to 46 g/L). The optimal salt concentration producing the highest levels of hydrogen, optimal substrate consumption and highest molar hydrogen yield is at 10 g/L NaCl (1.0% (w/v)).

scholarly journals Improvement of Hydrogen Production during Anaerobic Fermentation of Food Waste Leachate by Enriched Bacterial Culture Using Biochar as an Additive

2021 ◽  
Vol 9 (12) ◽  
pp. 2438
Author(s):  
Van Hong Thi Pham ◽  
Jaisoo Kim ◽  
Soonwoong Chang ◽  
Woojin Chung
Keyword(s):  
Renewable Energy ◽  
Hydrogen Production ◽  
Food Waste ◽  
Renewable Energy Sources ◽  
High Energy ◽  
Waste To Energy ◽  
Energy Sources ◽  
Hydrogen Yield ◽  
Food Waste Leachate ◽  
Waste Leachate

It has become urgent to develop cost-effective and clean technologies for the rapid and efficient treatment of food waste leachate, caused by the rapid accumulation of food waste volume. Moreover, to face the energy crisis, and to avoid dependence on non-renewable energy sources, the investigation of new sustainable and renewable energy sources from organic waste to energy conversion is an attractive option. Green energy biohydrogen production from food waste leachate, using a microbial pathway, is one of the most efficient technologies, due to its eco-friendly nature and high energy yield. Therefore, the present study aimed to evaluate the ability of an enriched bacterial mixture, isolated from forest soil, to enhance hydrogen production from food waste leachate using biochar. A lab-scale analysis was conducted at 35 °C and at different pH values (4, no adjustment, 6, 6.5, 7, and 7.5) over a period of 15 days. The sample with the enriched bacterial mixture supplemented with an optimum of 10 g/L of biochar showed the highest performance, with a maximum hydrogen yield of 1620 mL/day on day three. The total solid and volatile solid removal rates were 78.5% and 75% after 15 days, respectively. Acetic and butyrate acids were the dominant volatile fatty acids produced during the process, as favorable metabolic pathways for accelerating hydrogen production.

Performance comparison of a continuous-flow stirred-tank reactor and an anaerobic sequencing batch reactor for fermentative hydrogen production depending on substrate concentration

2005 ◽  
Vol 52 (10-11) ◽  
pp. 23-29 ◽  
Author(s):  
S.-H. Kim ◽  
S.-K. Han ◽  
H.-S. Shin
Keyword(s):  
Hydrogen Production ◽  
Substrate Concentration ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Hydrogen Yield ◽  
Anaerobic Sequencing Batch Reactor ◽  
Fermentative Hydrogen Production ◽  
Carbohydrate Degradation ◽  
Fermentative Hydrogen ◽  
Substrate Concentrations

This study was conducted to compare the performance of a continuous-flow stirred-tank reactor (CSTR) and an anaerobic sequencing batch reactor (ASBR) for fermentative hydrogen production at various substrate concentrations. Heat-treated anaerobic sludge was utilized as an inoculum, and hydraulic retention time (HRT) for each reactor was maintained at 12h. At the influent sucrose concentration of 5g COD/L, start-up was not successful in both reactors. The CSTR, which was started-up at 10g COD/L, showed stable hydrogen production at the influent sucrose concentrations of 10–60g COD/L during 203 days. Hydrogen production was dependent on substrate concentration, resulting in the highest performance at 30g COD/L. At the lower substrate concentration, the hydrogen yield (based on hexose consumed) decreased with biomass reduction and changes in fermentation products. At the higher substrate concentration, substrate inhibition on biomass growth caused the decrease of carbohydrate degradation and hydrogen yield (based on hexose added). The ASBR showed higher biomass concentration and carbohydrate degradation efficiency than the CSTR, but hydrogen production in the ASBR was less effective than that in the CSTR at all the substrate concentrations.

Effect of protein on biohydrogen production from starch of food waste

2008 ◽  
Vol 57 (7) ◽  
pp. 1031-1036 ◽  
Author(s):  
H. B. Ding ◽  
X. Y. Liu ◽  
O. Stabnikova ◽  
J.-Y. Wang
Keyword(s):  
Hydrogen Production ◽  
Food Waste ◽  
Organic Nitrogen ◽  
Volatile Fatty Acids ◽  
Biohydrogen Production ◽  
Food Wastes ◽  
Hydrogen Production Rate ◽  
Production Potential ◽  
Protein Ratio ◽  
Protein Rich

This study demonstrated the influence of protein on biohydrogen production from carbohydrates, especially starch, by using different combinations of two model food wastes, rice as starch-rich and soybean residue as protein-rich food waste. It was found the maximum specific hydrogen production potential, 0.99 mol H2/mol initial starch as glucose, and the maximum specific hydrogen production rate, 530 ml H2/h g-VS, occurred at a starch/protein ratio of 1.7. The protein content in the initial food waste not only provided buffering capacity to neutralize the volatile fatty acids as concurrent products but also enhanced the hydrogen production by providing readily available organic nitrogen such as soluble proteins and amino acids to microorganisms.

scholarly journals Effect of Substrate Concentration on Photo-Fermentation Bio-Hydrogen Production Process from Starch-Rich Agricultural Leftovers under Oscillation

2020 ◽  
Vol 12 (7) ◽  
pp. 2700 ◽  
Author(s):  
Haorui Zhang ◽  
Jiaolin Li ◽  
Quanguo Zhang ◽  
Shengnan Zhu ◽  
Shuai Yang ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Production Process ◽  
Production Rate ◽  
Substrate Concentration ◽  
Biogas Production ◽  
Fermentation Broth ◽  
Static Condition ◽  
Hydrogen Yield ◽  
Biogas Yield ◽  
Oscillation Condition

China has plenty of starch-rich agricultural leftovers, which can be degraded and further utilized for biogas production. Potato, which has more and more cultivated areas, was taken as a substrate. The pH, OD540, biogas yield, hydrogen yield, biogas production rate, and hydrogen production rate were determined to evaluate the effect of substrate concentration on the photo-fermentation bio-hydrogen production process under an oscillation condition. Results showed that the photo-fermentation period was extended to 264 h under oscillation, which was two times longer than the static condition. It was found that 8 g per 100 mL fermentation broth was the most suitable substrate concentration under oscillation, the cumulative hydrogen yield was 510 mL VS−1, and the hydrogen content was 38.36%.

scholarly journals BIOHYDROGEN PRODUCTION FROM WASTES OF PLANT AND ANIMAL ORIGIN VIA DARK FERMENTATION

2019 ◽  
Vol 27 (2) ◽  
pp. 101-113 ◽  
Author(s):  
Weronika Cieciura-Włoch ◽  
Sebastian Borowski
Keyword(s):  
Hydrogen Production ◽  
Sugar Beet ◽  
Dark Fermentation ◽  
Biohydrogen Production ◽  
Animal Origin ◽  
Hydrogen Yield ◽  
Vegetable Waste ◽  
Fruit And Vegetable ◽  
Kitchen Waste ◽  
High Hydrogen

This study investigated the batch experiments on biohydrogen production from wastes of plant and animal origin. Several substrates including sugar beet pulp (SBP), sugar beet leaves (SBL), sugar beet stillage (SBS), rye stillage (RS), maize silage (MS), fruit and vegetable waste (FVW), kitchen waste (KW) and slaughterhouse waste (SHW) including intestinal wastes, meat tissue, post flotation sludge were tested for their suitability for hydrogen production. Generally, the substrates of plant origin were found to be appropriate for dark fermentation, and the highest hydrogen yield of 280 dm3 H2/kg VS was obtained from fruit and vegetable waste. Contrary to these findings, slaughterhouse waste as well as kitchen waste turned out to be unsuitable for hydrogen production although their methane potential was high. It was also concluded that the combined thermal pretreatment with substrate acidification was needed to achieve high hydrogen yields from wastes.

scholarly journals Biohydrogen production by fermentive bacterium Clostridium sp. Tr2 using batch fermenter system controlled pH under dark fermentation

2018 ◽  
Vol 9 (1) ◽  
pp. 4-10
Author(s):  
Thi Thu Huyen Nguyen ◽  
Thi Yen Dang ◽  
Thuy Hien Lai
Keyword(s):  
Hydrogen Production ◽  
Alternative Energy ◽  
Fossil Fuels ◽  
Dark Fermentation ◽  
Gas Production ◽  
Biohydrogen Production ◽  
Great Promise ◽  
Hydrogen Yield ◽  
Promising Alternative ◽  
Fermentative Bacteria

Limitation of fuels reserves and contribution of fossil fuels to the greenhouse effect leads to develop anew, clean and sustainable energy. Among the various options, biohydrogen appears as a promising alternative energy source. The fermentative hydrogen production process holds a great promise for commercial processes. Hydrogen production by fermentative bacteria is a very complex and greatly influenced by pH. This paper presents biohydrogen production by bacterial strain Clostridium sp. Tr2. Operational pH strongly affected its hyrogen production. Its gas production rate as well as obtained gas product were roughly increase twice under controlled pH at 6 than non-controlled condition. Dark fermentation for hydrogen production of strain Tr2 was performed under bottle as well as automatic fermenter scale under optimal nutritional and environmental conditions at 30°C, initial pH at 6.5, then pH was controlled at 6 for bioreactor scale (BioFlo 110). Bioreactor scale was much better for hydrogen production of strain Tr2. Clostridium sp. Tr2 produced 0.74 L hydro (L medium)-1 occupying 72.6 % of total gas under bottle scale while it produced 2.94 L hydro (L medium)-1 occupying 95.82 % of total gas under fermenter scale. Its maximum obtained hydrogen yield of Clostridium sp. Tr2 under bioreactor scale Bioflo 110 in optimal medium with controlled pH 6 was 2.31 mol hydro (mol glucose)-1. Dự trữ nhiên liệu có giới hạn và việc sử dụng nhiên liêu hoá thạch góp phần không nhỏ gây hiệu ứng nhà kính dẫn đến cần phải phát triển năng lượng mới, sạch và bền vững. Trong số các giải pháp, hydro sinh học xuất hiện như một nguồn năng lượng thay thế đầy hứa hẹn. Quá trình lên men sản xuất hydro có tiềm năng lớn để áp dụng trong sản xuất thương mại. Tuy nhiên qúa trình này rất phức tạp và chịu ảnh hưởng lớn bởi pH. Nghiên cứu này trình bày sản xuất hydro sinh học do chủng vi khuẩn Clostridium sp. Tr2. Quá trình sản xuất hydro của chủng này bị ảnh hưởng mạnh mẽ bởi pH thay đổi trong quá trình lên men. Tốc độ tạo khí cũng như lượng khí thu được của chủng này tăng gần gấp đôi trong môi trường có duy trì pH ở pH 6 so với môi trường không kiểm soát pH. Quá trình lên men tối sản xuất hydro của chủng Tr2 được thực hiện ở quy mô bình thí nghiệm cũng như bình lên men tự động trong điều kiện môi trường tối ưu ở 30°C, pH ban đầu 6.5, ở qui mô bình lên men tự động (BioFlo 110), pH môi trường sau đó được duy trì ổn định ở pH 6. Lên men sản xuất hdyro của chủng Tr2 trong bình lên men tự động tốt hơn rất nhiều so với lên men trong bình thí nghiệm. Clostridium sp. Tr2 chỉ tạo ra được 0,74 L hydro (L medium)-1 chiếm 72,6 % tổng thể tích khí thu được ở điều kiện lên men bình thí nghiệm trong khi chủng này sản xuất được 2,94 L hydro (L medium)-1 chiếm 95,82 % tổng thể tích khí ở điều kiện lên men tự động. Sản lượng hydro thu được lớn nhất của chủng này trong bình lên men tự động BioFlo 110 trong trong môi trường tối ưu có kiểm soát pH tại pH 6 là 2,31 mol hydro (mol glucose)-1.

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