scholarly journals Microbial Lipid Production from Corn Stover by the Oleaginous Yeast Rhodosporidium toruloides Using the PreSSLP Process

Energies ◽  
2019 ◽  
Vol 12 (6) ◽  
pp. 1053 ◽  
Author(s):  
Xiaozan Dai ◽  
Hongwei Shen ◽  
Qiang Li ◽  
Kamal Rasool ◽  
Qian Wang ◽  
...  
Keyword(s):  
Corn Stover ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Rhodosporidium Toruloides ◽  
Biodiesel Production ◽  
Advanced Technology ◽  
Lignocellulosic Materials ◽  
Microbial Lipid ◽  
Lipid Yield ◽  
Microbial Lipid Production

Dry acid pretreatment and biodetoxification (DryPB) has been considered as an advanced technology to treat lignocellulosic materials for improved downstream bioconversion. In this study, the lipid production from DryPB corn stover was investigated by the oleaginous yeast Rhodosporidium toruloides using a new process designated prehydrolysis followed by simultaneous saccharification and lipid production (PreSSLP). The results found that prehydrolysis at 50 °C and then lipid production at 30 °C improved lipid yield by more than 17.0% compared with those without a prehydrolysis step. The highest lipid yield of 0.080 g/g DryPB corn stover was achieved at a solid loading of 12.5%. The fatty acid distribution of lipid products was similar to those of conventional vegetable oils that are used for biodiesel production. Our results suggested that the integration of DryPB process and PreSSLP process can be explored as an improved technology for microbial lipid production from lignocellulosic materials.

scholarly journals Exogenous l-proline improved Rhodosporidium toruloides lipid production on crude glycerol

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Rasool Kamal ◽  
Yuxue Liu ◽  
Qiang Li ◽  
Qitian Huang ◽  
Qian Wang ◽  
...  
Keyword(s):  
Cell Growth ◽  
Lipid Content ◽  
Crude Glycerol ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Rhodosporidium Toruloides ◽  
Biodiesel Production ◽  
Lipid Yield ◽  
Stress Agent ◽  
Glycerol Consumption

Abstract Background Crude glycerol as a promising feedstock for microbial lipid production contains several impurities that make it toxic stress inducer at high amount. Under stress conditions, microorganisms can accumulate l-proline as a safeguard. Herein, l-proline was assessed as an anti-stress agent in crude glycerol media. Results Crude glycerol was converted to microbial lipids by the oleaginous yeast Rhodosporidium toruloides CGMCC 2.1389 in a two-staged culture mode. The media was supplied with exogenous l-proline to improve lipid production efficiency in high crude glycerol stress. An optimal amount of 0.5 g/L l-proline increased lipid titer and lipid yield by 34% and 28%, respectively. The lipid titer of 12.2 g/L and lipid content of 64.5% with a highest lipid yield of 0.26 g/g were achieved with l-proline addition, which were far higher than those of the control, i.e., lipid titer of 9.1 g/L, lipid content of 58% and lipid yield of 0.21 g/g. Similarly, l-proline also improved cell growth and glycerol consumption. Moreover, fatty acid compositional profiles of the lipid products was found suitable as a potential feedstock for biodiesel production. Conclusion Our study suggested that exogenous l-proline improved cell growth and lipid production on crude glycerol by R. toruloides. The fact that higher lipid yield as well as glycerol consumption indicated that l-proline might act as a potential anti-stress agent for the oleaginous yeast strain.

Microbial lipid production by the oleaginous yeast Cryptococcus curvatus O3 grown in fed-batch culture

2011 ◽  
Vol 35 (5) ◽  
pp. 1906-1911 ◽  
Author(s):  
Jie Zhang ◽  
Xu Fang ◽  
Xiao-Ling Zhu ◽  
Yan Li ◽  
Hai-Peng Xu ◽  
...  
Keyword(s):  
Batch Culture ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Microbial Lipid ◽  
Cryptococcus Curvatus ◽  
Fed Batch ◽  
Fed Batch Culture ◽  
Microbial Lipid Production

scholarly journals Volatile Fatty Acids from Lipid-Extracted Yeast Provide Additional Feedstock for Microbial Lipid Production

Catalysts ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1009
Author(s):  
Gwon Woo Park ◽  
Seongsoo Son ◽  
Myounghoon Moon ◽  
Subin Sin ◽  
Kyoungseon Min ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Response Surface Methodology ◽  
Carbon Source ◽  
Response Surface ◽  
Volatile Fatty Acids ◽  
Lipid Production ◽  
Biodiesel Production ◽  
Microbial Lipid ◽  
Initial Ph ◽  
Microbial Lipid Production

Microbial lipid production from oleaginous yeasts is a promising process for the sustainable development of the microbial biodiesel industry. However, the feedstock cost poses an economic problem for the production of microbial biodiesel. After lipid extraction, yeast biomass can be used as an organic source for microbial biodiesel production. In this study, volatile fatty acids (VFAs), produced via anaerobic digestion of a lipid-extracted yeast (LEY) residue, were utilized as a carbon source for the yeast Cryptococcus curvatus. The response surface methodology was used to determine the initial pH and inoculum volume for the optimal VFA production. The experimental result for VFA concentration was 4.51 g/L at an initial pH of 9 and an inoculation 25%. The optimization results from the response surface methodology showed that the maximal VFA concentration was 4.58 g/L at an initial pH of 8.40 and an inoculation of 39.49%. This study indicates that VFAs from LEY can be used as a carbon source for microbial biodiesel production, with the potential to significantly reduce feedstock costs.

Towards high-throughput optimization of microbial lipid production: from strain development to process monitoring

2020 ◽  
Vol 4 (12) ◽  
pp. 5958-5969
Author(s):  
Dania Awad ◽  
Samer Younes ◽  
Matthias Glemser ◽  
Franz M. Wagner ◽  
Gerhard Schenk ◽  
...  
Keyword(s):  
Process Monitoring ◽  
High Throughput ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Throughput Optimization ◽  
Microbial Lipid ◽  
Strain Development ◽  
Fast Neutron Irradiation ◽  
Microbial Lipid Production ◽  
First Time

We describe, for the first time, the use of fast neutron irradiation for the rapid metabolic optimization of an oleaginous yeast. The approach established in this study provides a progressive path towards automatable strain development efforts.

Microbial lipid production by oleaginous yeasts on Laminaria residue hydrolysates

RSC Advances ◽  
2016 ◽  
Vol 6 (32) ◽  
pp. 26752-26756 ◽  
Author(s):  
Xibin Zhang ◽  
Hongwei Shen ◽  
Xiaobing Yang ◽  
Qian Wang ◽  
Xue Yu ◽  
...  
Keyword(s):  
Lipid Production ◽  
Biodiesel Production ◽  
Microbial Lipid ◽  
Microbial Lipids ◽  
Oleaginous Yeasts ◽  
Microbial Lipid Production

Laminaria residues, major wastes from the kelp industry, can be effectively converted by oleaginous yeasts into microbial lipids as potential feedstock for biodiesel production.

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