scholarly journals Photoinhibition of Streptococcus mutans Biofilm-Induced Lesions in Human Dentin by Violet-Blue Light

2019 ◽  
Vol 7 (4) ◽  
pp. 113 ◽  
Author(s):  
Grace Gomez Felix Gomez ◽  
Frank Lippert ◽  
Masatoshi Ando ◽  
Andrea F. Zandona ◽  
George J. Eckert ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Blue Light ◽  
In Vitro Study ◽  
Microtiter Plate ◽  
Significance Level ◽  
Supernatant Liquid ◽  
Colony Forming Units ◽  
Human Dentin ◽  
Mineral Loss

This in vitro study determined the effectiveness of violet-blue light on Streptococcus mutans (UA159) biofilm induced dentinal lesions. Biofilm was formed on human dentin specimens in a 96-well microtiter plate and incubated for 13 h in the presence of tryptic soy broth (TSB) or TSB supplemented with 1% sucrose (TSBS). Violet-blue light (405 nm) from quantitative light-induced fluorescence (QLFTM) was used to irradiate the biofilm. Supernatant liquid was removed, and the biofilm was irradiated continuously with QLF for 5 min twice daily with an interval of 6 h for 5 d, except with one treatment on the final day. Colony forming units (CFU) of the treated biofilm, changes in fluorescence (∆F; QLF-Digital BiluminatorTM), lesion depth (L), and integrated mineral loss (∆Z; both transverse microradiography) were quantified at the end of the fifth day. Statistical analysis used analysis of variance (ANOVA), testing at a 5% significance level. In the violet-blue light irradiated groups, there was a significant reduction (p < 0.05) of bacterial viability (CFU) of S. mutans with TSB and TSBS. Violet-blue light irradiation resulted in the reduction of ∆F and L of the dentinal surface with TSBS. These results indicate that violet-blue light has the capacity to reduce S. mutans cell numbers.

scholarly journals Adherence of Streptococcus mutans to Nanoceramic and Nanohybrid Resin Composites: An In vitro Study

2020 ◽  
pp. 128-133
Author(s):  
Krishnakanth Jaju ◽  
Iffat Nasim
Keyword(s):  
Streptococcus Mutans ◽  
Bacterial Adhesion ◽  
Resin Composite ◽  
In Vitro Study ◽  
Bacterial Suspension ◽  
Resin Composites ◽  
Colony Forming Units ◽  
Significant Difference ◽  
Test Control

Streptococcus mutans (S. mutans) is one of the cariogenic microorganisms. The restorative materials which harbor a biofilm with high number of S. mutans can accelerate the occurrence of dental caries. The purpose of this study was to evaluate the adherence of S. mutans to nanoceramic and nanohybrid resin composites. Fifteen discs of each material (Nanohybrid resin composite, Nanoceramic resin composite) were prepared, polished, and sterilized in a gamma radiation chamber. Specimens were exposed to the S. mutans bacterial suspension (0.5 McFarland) and were incubated for 4 hours. Specimens were rinsed and sonicated in normal saline, 10 μl of the obtained suspension was cultured in a sterile blood agar medium. After 24 hours, the number of colony forming units of S. mutans was counted. A sterility test control was considered for each group of materials. The data was analyzed by Independent t test. The means and standard deviations of the logarithmic counts of the colonies on the surfaces of nanohybrid resin composites and nanoceramic resin composite were equal to 3.2±0.87 and 2.8±0.324 respectively. Ceram X Universal did not show any significant difference in the bacterial adhesion compared to Filtek Z350XT. Both composites showed similar behaviour in terms of S. mutans colonization in a simple biofilm formation model.

scholarly journals Streptococcus mutans adherence to conventional and self-ligating brackets: an in vitro study

2021 ◽  
Vol 26 (6) ◽  
Author(s):  
Murilo Fernando Neuppmann FERES ◽  
Fernanda VICIONI-MARQUES ◽  
Fábio Lourenço ROMANO ◽  
Marina Guimarães ROSCOE ◽  
Vinícius Matsuzaki de SOUZA ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Bacterial Adhesion ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bacterial Suspension ◽  
Colony Forming Units ◽  
Conventional Brackets ◽  
Adhesion Rate ◽  
Post Hoc

ABSTRACT Introduction: Although self-ligating brackets presumably provide better hygiene conditions, no consensus has been reached so far. Objective: Therefore, the objective of this study was to evaluate, in an in vitro experimental design, the adherence of Streptococcus mutans (SM) in self-ligating and conventional brackets of different manufacturers and ligature types. Methods: Four commercial brands of maxillary premolar metal brackets were tested (Abzil®; Morelli®; 3M Unitek®; and GAC®). Each one was subdivided into three groups, which varied according to the type of ligature and bracket model (metallic, elastic, and self-ligating), totalizing twelve groups, composed of six brackets each. Previously sterilized brackets were initially immersed in saliva for one hour, and subsequently washed and added in a bacterial suspension, maintained in aerobiosis for 72 hours. The adhered bacteria were then separated and quantified by colony forming units (CFU/mL) counting after 48 hours of growth. The groups were compared by Kruskal-Wallis and Dunn post-hoc tests (p< 0.05). Results: Regardless of the commercial brand, self-ligating brackets had significantly less CFU/mL. However, according to comparisons performed within each commercial brand, only Abzil® self-ligating brackets had significantly lower biofilm adhesion. Among all of the self-ligating models, GAC® brackets presented the highest bacterial adhesion rate. Conclusions: Self-ligating brackets are likely to present lower rates of biofilm adhesion. Particularly, Abzil® and GAC® self-ligating brackets are less likely to accumulate biofilm. Although such results are derived from an in vitro study, practitioners might acknowledge findings concerning bacterial adhesion as one of the relevant features to be considered during bracket selection.

Cariogenic Potential of Infant Formulas–An In Vitro Study

2019 ◽  
Vol 43 (3) ◽  
pp. 181-184
Author(s):  
Nurit Dagon ◽  
Ronit Bar-Ness Greenstein ◽  
Yardena Mazor ◽  
Tal Ratson
Keyword(s):  
Streptococcus Mutans ◽  
In Vitro Study ◽  
Distilled Water ◽  
Water Type ◽  
Infant Formulas ◽  
Anaerobic Conditions ◽  
Bacterial Fermentation ◽  
Colony Forming Units ◽  
Significant Difference

Objectives: To compare the in vitro cariogenic potential of two infant formulas (IF, for ages 6–12 months) processed with sterile distilled water with or without 1 ppm fluoride. Study design: Nine specimens in each water type were inoculated with Streptococcus mutans (SM) suspension. The specimens were then divided into one group of samples cultured immediately on Mitis salivarius (MS) agar plates (T0) and another group of specimens cultured on MS agar plates after incubation at 37°C for 4 hours in anaerobic conditions (T4). Six-fold dilutions of each sample were incubated for 48 hours, and colony-forming units (CFUs) of SM were numerated. The pH changes associated with bacterial fermentation of each of the suspensions were measured at T0 and at T4 following incubation. Results: The pH was lower at T4 than at T0 in both IF. The SM colonies increased significantly at T4 compared to T0 in both IF (P &lt; 0.001). There was no significant difference between the CFUs of the mediums at T0 and T4 or relation between SM colonies of IF based on fluoridated and non-fluoridated distilled water. Conclusion: The cariogenic potential was not significantly different between two IF prepared with fluoridated or non-fluoridated distilled water.

scholarly journals Photodynamic inactivation of planktonic cultures of Streptococcus mutans using erythrosine irradiated by LED

2020 ◽  
Vol 23 (2) ◽  
Author(s):  
Maria Ângela Lacerda Rangel Esper ◽  
Junia Oliveira Barbosa ◽  
Janaína De Araújo Alvarenga ◽  
Juliana Campos Junqueira ◽  
Alessandra nara De Souza Rastelli ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Light Emitting Diode ◽  
In Vitro Study ◽  
Control Group ◽  
Photodynamic Inactivation ◽  
Experimental Conditions ◽  
Light Emitting ◽  
Colony Forming Units ◽  
Led Irradiation

Objective: The aim of this in vitro study was to evaluate the efficacy of photodynamic inactivation (PDI) with erythrosine (E), using a light-emitting diode (LED) on planktonic cultures of Streptococcus mutans. Material and Methods: A Streptococcus mutans strain (UA 159) was used to prepare the suspensions containing 107 cells/mL, which was tested under different experimental conditions: a) LED irradiation in the presence of erythrosine as a photosensitizer (E+L+); b) LED irradiation only (P-L+); c) treatment with erythrosine only (E+L-); and d) no LED irradiation or photosensitizer (P) treatment, which served as a control group (P-L-). After treatment, strains were seeded onto MSBS agar for determination of the number of colony-forming units (CFU/mL). Results: The results were submitted to analysis of variance and the Tukey test (p < 0.05). No reduction in the number of CFU/mL was observed in the treatment group with erythrosine (E+L+) when compared to the control (P-L-). Conclusion: PDI using erythrosine did not reduce the number of CFUs per millimeter within the parameters in this study.KEYWORDSErythrosine; Decay; Photodynamic inactivation; Light-emitting diode.

scholarly journals Effect of Probiotic Enterococcus durans on the Adhesion of Clinically Isolated Streptococcus mutans

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Sayedeh Fatemeh Mousavi ◽  
Amjad Ahmadi ◽  
Rashid Ramazanzadeh ◽  
Bijan Nuori ◽  
Faranak Shafiee
Keyword(s):  
Streptococcus Mutans ◽  
Bacterial Colonization ◽  
Statistical Significance ◽  
In Vitro Study ◽  
Microtiter Plate ◽  
Inhibitory Effect ◽  
Vitro Study ◽  
Clinical Samples ◽  
Enterococcus Durans

Background: Streptococcus mutans is the most cariogenic microorganism with high adherence ability to the tooth surfaces. The probiotics have attracted attention as a new and bioecological technique to inhibit oral bacterial colonization. Enterococcal strains, such as Enterococcus durans, are abundant in nature and can be identified as a probiotic. Objectives: Since the reduction of adhesion can be an effective way to decrease the cariogenic potential of S. mutans, the present study aimed to evaluate the inhibitory effect of E. durans on the adhesion of S. mutans. Methods: In this in vitro study, the standard strain bacteria of probiotic E. durans and Streptococcus mutans and 12 clinical samples of S. mutans were used. The ability of S. mutans biofilm formation was assessed. Then, the effect of E. durans on S. mutans adhesion was determined via microtiter plate technique by two methods: (1) Adding a mixed suspension of E. durans and S. mutans simultaneously; (2) adding E. durans 30 minutes before the inoculation of S. mutans to the system. The data were analyzed with SPSS 20 by using paired t-test, and statistical significance was set at P < 0.05. Results: The results showed a decrease in adhesion in the presence of E. durans, with the use of both methods (P < 0.05). Conclusions: Based on the results of this in vitro study, the use of probiotic E. durans decreased the adhesion of S. mutans.

scholarly journals Apical extrusion of bacteria following the use of reciprocating single-file and rotary multi-file instrumentation systems in oval root canals

2020 ◽  
Vol 23 (4) ◽  
pp. 8p
Author(s):  
Safa Kurnaz ◽  
Irem Bag ◽  
Aysel Gulbandilar
Keyword(s):  
Statistical Significance ◽  
In Vitro Study ◽  
Control Group ◽  
Significance Level ◽  
Root Canals ◽  
Single File ◽  
Colony Forming Units ◽  
Apical Extrusion ◽  
Significant Difference

Objective: All instrumentation techniques and instruments are associated with apical extrusion during chemomechanical preparation, and this causes postoperative pain and flare-up. However, it is controversial whether reciprocal systems or rotary systems cause more apical extrusion. The objective of this in vitro study was to determine the differences in the amounts of apically extruded bacteria (AEB) associated with nickel-titanium rotary and reciprocating systems when used in oval-shaped root canals. Material and Methods: Seventy human mandibular premolar teeth with oval-shaped canals were randomly assigned to four experimental groups (15 teeth in each group) and one control group (10 teeth). The root canals were contaminated with Enterococcus faecalis and instrumented using two full-sequence rotary instruments (ProTaper Universal [PTU] and ProTaper Next [PTN]) and two reciprocating single-file instruments (Reciproc [R] and WaveOne [WO]). A 0.9% NaCl solution was used as an irrigant, and the bacterial extrusion was quantified as the number of colony-forming units for each sample. The results were statistically analyzed using the Kruskal-Wallis one-way analysis of variance and the Mann-Whitney U test, and the statistical significance level was set at p <0.05. Results: The R system was associated with the highest amount of bacterial extrusion (p <0.05). The PTU system caused more bacterial extrusion than the PTN and WO systems (p <0.05). There was no significant difference between the PTN and WO systems (p >0.05). Conclusions: All instrumentation techniques caused apical bacterial extrusion. The instrument design and preparation techniques affect the number of extruded bacteria.KEYWORDS Apical extrusion; Bacteria; Endodontics; Root canal preparation.

Exploring the anti-caries properties of baicalin against Streptococcus mutans: an in vitro study

Biofouling ◽  
2021 ◽  
pp. 1-9
Author(s):  
Arval Viji Elango ◽  
Sahana Vasudevan ◽  
Karthi Shanmugam ◽  
Adline Princy Solomon ◽  
Prasanna Neelakantan
Keyword(s):  
Streptococcus Mutans ◽  
In Vitro Study ◽  
Vitro Study

ERYTHROSINE AS A PHOTOSENSITIZER FOR ANTIMICROBIAL PHOTODYNAMIC THERAPY WITH BLUE LIGHT-EMITTING DIODES – AN IN VITRO STUDY

2021 ◽  
pp. 102445
Author(s):  
Marcela Leticia Leal Gonçalves ◽  
Elaine Marcílio Santos ◽  
Ana Cláudia Muniz Renno ◽  
Anna Carolina Ratto Tempestini Horliana ◽  
Matheus de Almeida Cruz ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Blue Light ◽  
Light Emitting Diodes ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antimicrobial Photodynamic Therapy ◽  
Light Emitting

scholarly journals Effectiveness of a silicon-based root canal sealer for filling of simulated lateral canals

2007 ◽  
Vol 18 (1) ◽  
pp. 20-23 ◽  
Author(s):  
João Vicente Baroni Barbizam ◽  
Matheus Souza ◽  
Doglas Cecchin ◽  
Jakob Dabbel
Keyword(s):  
Root Canal ◽  
In Vitro Study ◽  
Root Canal Sealer ◽  
Human Teeth ◽  
Significance Level ◽  
Working Length ◽  
Group 3 ◽  
Group 2 ◽  
Silicon Based

The purpose of this in vitro study was to evaluate the ability of a silicon-based root canal sealer, compared to zinc oxide and eugenol and an epoxy resin-based sealers, for filling of simulated lateral canals. Thirty extracted single-rooted human teeth were selected, conventional access was made and the working length was established 1 mm from the apical foramen. Three simulated lateral canals, one in each root third (coronal, middle and apical) were prepared in both the mesial and distal surfaces of each tooth using a size 15 reamer adapted to a low-speed handpiece. Each root canal was instrumented using ProTaper rotary files up to file F3 at the working length, and then irrigated with 2.5% NaOCl followed by EDTA. The teeth were assigned to 3 groups (n=10), according to the root canal sealer: Roeko Seal (Group 1), Sealer 26 (group 2) and Grossman's sealer (Group 3). Gutta-percha cold lateral condensation technique was performed in all groups. Postoperative radiographs were taken and the images were projected for evaluation of the quality of lateral canal filling. Data were submitted to statistical analysis by Kruskal Wallis test at 5% significance level. The results showed that Grossman's sealer filled a larger number of lateral canals than Roeko Seal (p<0.05) and Sealer 26 (p<0.01). It may be concluded that Roeko Seal silicone-based root canal sealer was not as effective as the Grossman's sealer for filling of simulated lateral canals. The lateral canals localized in the apical third of the root were more difficult to be filled.

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