scholarly journals Impact of Different Irrigant Agitation Methods on Bacterial Elimination from Infected Root Canals

2019 ◽  
Vol 7 (3) ◽  
pp. 64 ◽  
Author(s):  
Wajih Hage ◽  
Roeland J. G. De Moor ◽  
Désirée Hajj ◽  
Germain Sfeir ◽  
Dolla K. Sarkis ◽  
...  

Activation techniques are essential for root canal disinfection but may result in incomplete removal of bacteria. The aim of our study was to assess the antibacterial action of sonically, ultrasonically and laser-activated irrigation and 5.25% sodium hypochlorite (NaOCl) on Enterococcus faecalis in an infected tooth. Forty-four extracted mandibular premolars were mechanically prepared, sterilized, and inoculated with E. faecalis for 1 week. Bacterial counts after inoculation were evaluated in 4 randomly chosen teeth, remaining root canals were divided into 4 groups. Group A: laser-activated irrigation by photon-induced photoacoustic streaming, Group B: the sonic irrigation by EDDY, Group C: ultrasonic irrigation by EndoUltra, and Group D: 5.25% NaOCl. Colony forming unit (CFU) counts were measured and Kolmogorov–Smirnov, Wilcoxon, Kruskal–Wallis and Mann–Whitney tests were used to determine differences. The mean of CFU was found to significantly decrease in group D, 2110 ± 1015.93 (p < 0.001). Changes in measurement levels followed the same trend over time in groups A 27.40 ± 30.15, B 81.3 ± 85.68 and C 44.40 ± 67.12 (p = 0.141). The average CFU after irrigation in all groups was significantly greater than 0. Within the limitations of this study, all activation techniques were superior to NaOCl 5.25% in reducing E. faecalis from the infected tooth model.

2020 ◽  
Author(s):  
Obeagu Emmanuel Ifeanyi

The hematological features of Gongronema latifolium, aqueous leaves extract was evaluated using standard methods. After 10 days of consecutive infusions into 9 experimental animals (rabbits). The rabbits were monitored and the following parameters determined; hemoglobin (HB), PCV, Platelet, WBC, Differential White Blood Cell. The Rabbits were grouped into 4, one consisting of control (group A), group B was fed with 0.5 mg/kg, group C with 1.0 mg/kg, and Group D with 1.5mg/kg of the aqueous extract of Gongronemalatifolium. The mean values obtained for hemoglobin estimation for the control group is 5.9 ± 4.1 g/dl, 9.1 ± 2.9 g/dl for group B 10.2 ± 1.8 g/dl for group C and 12.8 ± 0.1 g/dl for group D with no significant increase on the PCV estimation, the mean value for the control (group A) is 17.7 ± 12.3%, 27.3 ± 8.7% for group B, group C (30.6 ± 5.4%) and D (28.4 ± 0.3) show increase that statistically significant (p > 0.01). the platelet counts of group C (600 ± 0 x 109/L) and D(600 ± 0 x 109/L) show significant increase (p > 0.01) when compared with the control (600 ± 00). But the platelet value of group B (550 ± 50 x 109/L) shows no difference. No significant changes were observed in the White Blood Counts of the test groups B (3.5 ± 0.5 x 109/L), C (1.9 + 2.1 x 109/L) and D(3.6 ± 0.4) when compared with the control group (2.9 ± 1.9). The values obtained from the differential White Blood Counts (Neutrophils, Lymphocytes, Eosinophils and Monocytes) were not significant. Therefore, Gongronemalatifolium, when properly taken as a nutritional diet, causes beneficial changes on hemoglobin, packed cell volumes and platelet counts of consumers.


2019 ◽  
Vol 7 (1) ◽  
pp. 2 ◽  
Author(s):  
Marc Krikor Kaloustian ◽  
Walid Nehme ◽  
Claire El Hachem ◽  
Carla Zogheib ◽  
Nabil Ghosn ◽  
...  

We assessed the efficiency of two shaping file systems and two passive ultrasonic irrigation (PUI) devices for removing filling material during retreatment. The mesial canals from 44 extracted mandibular molars were prepared and obturated. The teeth were randomly divided into two groups, and then one group was retreated with Reciproc R25 (VDW, Munich, Germany) (n = 44) and the other group was retreated with 2Shape (TS, Micro Mega, Besançon, France) (n = 44). A micro-computed tomography (CT) scan was taken before and after the retreatment to assess the volume of the filling material remnants. The teeth were then randomly divided into four groups to test two different PUI devices: Irrisafe (Satelec Acteon Group, Merignac, France) and Endo Ultra (Vista Dental Products, Racine, WI, USA). The teeth in Group A were retreated with 2Shape to test the Endo Ultra (n = 22) device, the teeth in Group B were retreated with 2Shape in order to test the Irrisafe (n = 22) device, the teeth in Group C were retreated with Reciproc to test the Endo Ultra (n = 22) device, and Group D was retreated with Reciproc to test the Irrisafe (n = 22) device. A third micro-CT scan was taken after the retreatment to test the PUIs. The percentage of Gutta-Percha (GP) and sealer removed was 94.75% for TS2 (p < 0.001) and 89.3% for R25 (p < 0.001). The PUI significantly enhanced the removal of the filling material by 0.76% for Group A (p < 0.001), 1.47% for Group B (p < 0.001), 2.61% for Group C (p < 0.001), and by 1.66% for Group D (p < 0.001). 2Shape was more effective at removing the GP and sealer during retreatment (p = 0.018). The supplementary approach with PUI significantly improved filling material removal, with no statistical difference between the four groups (p = 0.106).


2005 ◽  
Vol 13 (2) ◽  
pp. 147-151 ◽  
Author(s):  
Daniela Ribeiro da Silva ◽  
Ivaldo Gomes de Moraes

The purpose of the present study was to evaluate the influence of some auxiliary agents of biomechanical preparation of the root canal on the filling of artificial lateral canals in extracted human teeth. A total of eighty single-rooted teeth were employed, which were submitted to preparation of three artificial lateral canals in one of the proximal aspects at the cervical, middle and apical thirds, besides one in the buccal aspect. The main canals were prepared by Profile 0.4 rotary instruments through the crown-down technique and irrigated with the irrigants investigated, as follows: Group A - 1% sodium hypochlorite and final irrigation with trisodium EDTA for 5 minutes; Group B - Endogel (2% chlorhexidine gel); Group C - Endo PTC and Dakin's solution and final irrigation with Tergentol- Furacin; and Group D - File Eze. The root canals were obturated by the Tagger's hybrid technique and then radiographed for assessment of the penetration rate of the filling materials in the lateral canals. Analysis of the results demonstrated no statistically significant difference (p<0.05) between the irrigants both to each other and as regards the location (thirds and aspects) of the artificial lateral canals.


2014 ◽  
Vol 42 (4) ◽  
Author(s):  
Maria Angelica Zoppi ◽  
Ambra Iuculano ◽  
Giovanni Monni

AbstractWe investigated the umbilical vein volume flow (UVVF) at 11–14 weeks in monochorionic-diamniotic (MCDA) twins, focusing on the occurrence of complications. We considered 87 MCDA pregnancies. We used “E-flow” to detect the umbilical vein and measured the mean velocities and diameters of veins, also calculating the UVVF. Pregnancies were divided into four groups: a (twin-to-twin transfusion, TTTS); b (selective intrauterine growth restriction, sIUGR); c (discordance of fluid, DF); and d (with “normal” outcome). The main outcome of the study was the assessment of the difference of UVVF between twin 1 and twin 2 (larger and smaller twin). In eight of eight pairs of group a and seven of seven pairs of group b, the UVVF of fetuses 1 were significantly different than fetuses 2 (P<0.05). The UVVFs of fetuses 2 of group a were significantly lower in respect to the UVVFs of fetuses 2 of group d (P<0.05). We provide evidence of an important difference in UVVF in pairs that successively developed TTTS or sIUGR. The smaller fetuses of group a (TTTS) showed a lower venous return than fetuses 2 of group d (normal). First-trimester UVVF is lower in fetuses with a smaller crown-rump length in twin pairs at risk of TTTS or IUGR.


2020 ◽  
Author(s):  
Ben Xu ◽  
Yi-ji Peng ◽  
Guo-Zhong Ma ◽  
Qian Zhang

Abstract Background: To introduce a novel “three-port” trocar placement technique for laparoscopic radical prostatectomy (LRP) in prostate cancer (PCa) patients.Methods: We retrospectively reviewed 300 patients with PCa who received surgical treatment between November 2010 and June 2015 at our institution. They were divided into group A: three-port LRP, group B: conventional four-five port LRP, group C: open RP (ORP) and group D: robotic-assisted RP (RARP). A learning curve was analyzed by dividing patients of group A into the early and late stages.Results: All groups were comparable with regard to the preoperative characteristics except for the relatively smaller prostate volume in group A. The three-port LRP operations were performed successfully with only 8 cases conversion to the conventional LRP. None of any severe complications or conversion to ORP occurred. In group A, the mean operative time (OT) duration was 113.8min, the mean estimated blood loss (EBL) was 94.2ml, the mean drainage days was 4.0d, the mean hospitalization was 5.1d, and 27.8% of the prostate specimen margins (PSM) were positive. The differences of OT, EBL, drainage days, hospitalization and transfusion in group A were statistically significant among the majority of the other groups (p<0.05). After undergoing the early stages of a learning curve analysis in three-port LRP, the EBL was obviously decreased.Conclusions: Three-port LRP is a novel technique that exhibits superior intraoperative advantages to the conventional LRP. Due to its less OT, EBL, drainage days, hospitalization and transfusion with a shorter learning curve, it should be recommended and popularized in the clinical practice!


2013 ◽  
Vol 2 (2) ◽  
pp. 8-12
Author(s):  
Rubina Qasim ◽  
Humaira Naushaba ◽  
Md Enayet Ullah ◽  
Hasna Hena ◽  
Hosna Ara Perven

Background : Ovarian diseases and infertility are the very common health problems among the female population. Alteration in the volume of the ovary occurs throughout the reproductive life which may predispose to ovarian diseases such as cyst, polycystic ovary and ovarian carcinoma. Moreover, the reduction in ovarian volume leading to decrease the fertility of a women also. So the present study is designed to assess the volume of the ovary in Bangladeshi women in different age groups which will provide a complete and standarize the data in Bangladeshi population and also compare the data with other countries. It will provide resourcefull information which may further determine the ovarian reserve and reproductive age of female for improving the accurate diagnosis and management of ovarian diseases as well as infertility problems. Study design: Cross sectional and analytical type of study. Materials: The present study was performed on 65 cadaveric ovaries of both side in Bangladeshi female age ranging from 5 years to 65 years. Methods: The samples were divided into four different age groups. They were group A or prepubertal group (5-12years), group B or reproductive group (13-45 years), group C or perimenopausal group (46-51years) and group D or postmenopausal group (52-65years). Results: The mean (±SD) volumes were 2.84 + 0.22 milliliter and 2.83±0.24 milliliter in group A, 8.64 + 0.89 milliliter and 8.61± 0.89 milliliter in group B, 6.85 + 0.79 milliliter and 6.84±0.76 milliliter in group C and 2.62 + 0. 52 milliliter and 2.61± 0.50 milliliter in group D in the right & left ovaries respectively. The highest mean volume was observed in group B and lowest mean volume was in group D in both ovaries. Statistically highly significant difference (P<0.001) were found when group A was compared with group B & C, group B was compared with group C & D and group C was compared with group D. But when group A was compared with group D, it was not significant (P<0.50).The mean difference in volumes of right and left ovary between group A, group B, group C and group D were statistically not significant. Conclusion: In this study, volume of the ovary does not vary in between right and left ovary in any age group. DOI: http://dx.doi.org/10.3329/updcj.v2i2.15482 Update Dent. Coll. j: 2012; 2 (2): 08-12


2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Shan Chen ◽  
Yu-Tong Jin ◽  
Zheng-Yang Zhu ◽  
Ling-Tao Wu ◽  
Ping Yang ◽  
...  

Objective. To investigate the site of action of sinapine thiocyanate (ST), following acupoint herbal patching (AHP). Methods. Twenty Wistar rats were randomized into five groups (groups A, B, C, D, and E), and all groups received the same AHP in vivo. Skin samples were excised at 2 h, 4 h, 6 h, 10 h, and 26 h after AHP administration from group A to group E separately and the concentrations of ST in the skin were determined using a liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) method. A pharmacokinetic profile of ST following AHP was performed at the same time in a group of five Wistar rats to detect plasma levels at the same time intervals. Results. The mean ± SD ST concentrations (ng/ml) at 2 h (group A), 4 h (group B), 6 h (group C), 10 h (group D), and 26 h (group E) after AHP administration were 250.01±61.99, 61.01±30.41, 40.12±26.94, 78.66±59.43, and 19.55±18.95, respectively. No ST was detected in rats’ plasma samples at the same time points. Conclusions. The site of action of ST following AHP is in the skin.


2020 ◽  
Author(s):  
Ben Xu ◽  
Hai-feng Song ◽  
Cheng Luo ◽  
Qian Zhang

Abstract Background: To introduce a novel “three-port” trocar placement technique for laparoscopic radical prostatectomy (LRP) in prostate cancer (PCa) patients.Methods: We retrospectively reviewed 300 patients with PCa who received surgical treatment between November 2010 and June 2015 at our institution. They were divided into group A: three-port LRP, group B: conventional four-five port LRP, group C: open RP (ORP) and group D: robotic-assisted RP (RARP). A learning curve was analyzed by dividing patients of group A into the early and late stages.Results: All groups were comparable with regard to the preoperative characteristics except for the relatively smaller prostate volume in group A. The three-port LRP operations were performed successfully with only 8 cases conversion to the conventional LRP. None of any severe complications or conversion to ORP occurred. In group A, the mean operative time (OT) duration was 113.8 min, the mean estimated blood loss (EBL) was 94.2 ml, the mean drainage days was 4.0d, the mean hospitalization was 5.1 d, and 27.8% of the prostate specimen margins (PSM) were positive. The differences of OT, EBL, drainage days, hospitalization and transfusion in group A were statistically significant among the majority of the other groups (p<0.05). After undergoing the early stages of a learning curve analysis in three-port LRP, the EBL was obviously decreased.Conclusions: Three-port LRP is a novel technique that exhibits superior intraoperative advantages to the conventional LRP. Due to its less OT, EBL, drainage days, hospitalization and transfusion with a shorter learning curve, it should be recommended and popularized in the clinical practice!


Mediscope ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 1-6
Author(s):  
Dilruba Siddiqua ◽  
Abu Sadat Mohammad Nurunnabi ◽  
Fatema Johora ◽  
Hasna Hena ◽  
Sunjida Shahriah ◽  
...  

Background: The human adrenal gland shows a distinct proportion of two histological zones, named the cortex and the medulla, from outer inwards, in its histological appearance with differences in its cellular components and functions. Objective: The present study aims to see the variation in the proportion of the cortex and the medulla of the adrenal glands with age in a Bangladeshi population. Methods: A cross-sectional, descriptive study was done in the Department of Anatomy, Dhaka Medical College, Dhaka, from July 2008 to June 2009, based on collection of 140 postmortem human adrenal glands from 70 unclaimed dead bodies, in the Department of Forensic Medicine, Dhaka Medical College, Dhaka. The samples were divided into four age-groups including A (11-20 years), B (21-30 years), C (31-40 years) & D (41-60 years). Histological slides were prepared by using routine haematoxylin and eosin stain (H & E). Five best prepared slides from each group were examined under light compound microscope with low magnification. The thickness of adrenal cortex and medulla were measured by using ocular and stage micrometer and then converted into percentage volume. Results: The mean volume of the right adrenal cortex were found 83.64±3.71% in group A, 83.90±1.75% in group B, 83.74±5.78% in group C, 84.80±3.82% in group D, while the mean volume of the corresponding medulla were found 18.16±3.23% in group A, 15.70±2.71% in group B, 16.26±3.97% in group C, 16.20±4.04% in group D. The mean volume of the left adrenal cortex were found 84.64±3.49%, 84.90±1.75%, 84.20±3.40%, 85.44±2.66% in group A, B, C and D respectively, while the mean volume of the corresponding medulla were found 17.26±3.84%, 17.00±2.37%, 16.00±3.20% and 14.36±2.33% in group A, B, C and D respectively. The differences among the groups were not statistically significant. Conclusion: No difference was found in the proportion of the cortex and the medulla of adrenal gland in different age-groups. Mediscope Vol. 8, No. 1: January 2021, Page 1-6


2000 ◽  
Vol 93 (5) ◽  
pp. 766-773 ◽  
Author(s):  
Seung-Chyul Hong ◽  
Kwan-Soo Kang ◽  
Dae Won Seo ◽  
Seung Bong Hong ◽  
Munhyang Lee ◽  
...  

Object. Surgical treatment of cortical dysplasia (CD) together with intractable seizures is challenging because both visualization and localization of the lesion are difficult, correlation with seizure foci requires comprehensive study, and the surgical outcomes reported thus far are unsatisfactory. The authors report their experience in the surgical treatment of CD classified according to a surgical point of view.Methods. The definition of CD used in this study was a dysplastic lesion visible on magnetic resonance (MR) images or a lesion that, although not visible on MR images, was diagnosed as moderate-to-severe dysplasia by using pathological analysis. During the last 4.5 years, the authors treated 36 patients with intractable epilepsy accompanied by CD. They divided the 36 cases of CD into four characteristic groups: Group A, diffuse bilateral hemispheric dysplasia; Group B, diffuse lobar dysplasia; Group C, focal dysplasia; and Group D, a moderate to severe degree of CD with a normal appearance on MR images. All but one patient in Group C were monitored in the epilepsy monitoring unit by using subdural electrodes for seizure localization and functional mapping.The incidence of CD among a cohort of 291 patients who had undergone epilepsy surgery at the authors' center during the study period was 12.4%. The mean age of the 36 patients was 21.3 years and the mean age at seizure onset was 8.5 years. The mean follow-up period was 26 months. Twenty-six patients (72.2%) belonged to Engel Class I or II (20 and six, respectively). There were five cases in Group A, nine in Group B, nine in Group C, and 13 in Group D. Patients in Groups A and B were significantly younger at seizure onset and had significantly poorer surgical outcomes compared with patients in Groups C and D (p < 0.05). If outcome is compared on the basis of the extent of removal of CD, patients in whom CD was completely removed had significantly better outcomes than those in whom CD was only partially removed (p < 0.001).Conclusions. The authors conclude that intractable epilepsy accompanied by CD can be treated surgically using comprehensive preoperative approaches. Deliberate resective procedures aimed at complete removal of dysplastic tissue ensure excellent seizure control without permanent neurological deficit.


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