scholarly journals Phosphophoryn and Dentin Sialoprotein Effects on Dental Pulp Cell Migration, Proliferation, and Differentiation

2018 ◽  
Vol 6 (4) ◽  
pp. 70 ◽  
Author(s):  
Shu-Feng Chuang ◽  
Yu-Hsuan Chen ◽  
Peter Ma ◽  
Helena Ritchie
Keyword(s):  
Cell Proliferation ◽  
Cell Migration ◽  
Dental Pulp ◽  
Matrix Protein ◽  
Type I ◽  
Pulp Cell ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Dental Pulp Cell ◽  
Dentin Sialoprotein

Phosphophoryn (PP) and dentin sialoprotein (DSP) are two of the most abundant dentin matrix non-collagenous proteins, and are derived from dentin sialoprotein-phosphophoryn (DSP-PP) mRNA. Mutations in the DSP-PP gene are linked to dentinogenesis imperfecta II and III. Previously, we reported transient DSP-PP expression in preameloblast cells first, followed by co-expression in preameloblasts and young odontoblasts, and finally sustained expression in odontoblasts. This phenomenon raised the possibility that DSP/PP proteins secreted by preameloblasts might promote dental pulp cell migration toward the dental pulp border and promote dental pulp cell differentiation. To examine the effects of DSP/PP proteins on dental pulp cell development, we investigated:(1) native PP effects on dental pulpcell migration and matrix protein expression; and (2) recombinant DSP/PP protein effects on cell proliferation and differentiation. We found that PP promoted cell migration and the expression of high levels of Col type I and PP in dental pulp cells. The addition of recombinant DSP/PP proteins affected cell proliferation and differentiation in a dental pulp cell line. These findings strongly suggest that DSP/PP may modulate cell migration, cell proliferation and differentiation, thus leading to dentin formation. DSP/PP protein may be useful clinically for pulp tissue regeneration.

scholarly journals Lin28 promotes dental pulp cell proliferation via upregulation of cyclin-dependent proteins and interaction with let-7a/IGF2BP2 pathways

2019 ◽  
Vol 113 ◽  
pp. 108742 ◽  
Author(s):  
Yan Liu ◽  
Ning Dong ◽  
Jiyu Miao ◽  
Chenxing Li ◽  
Xiaofei Wang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Dental Pulp ◽  
Pulp Cell ◽  
Dental Pulp Cell

scholarly journals Silencing hyperoxia-induced C/EBPα in neonatal mice improves lung architecture via enhanced proliferation of alveolar epithelial cells

2011 ◽  
Vol 301 (2) ◽  
pp. L187-L196 ◽  
Author(s):  
Guang Yang ◽  
Maurice D. Hinson ◽  
Jessica E. Bordner ◽  
Qing S. Lin ◽  
Amal P. Fernando ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Type I ◽  
Epithelial Cell Proliferation ◽  
Newborn Mice ◽  
Alveolar Epithelial ◽  
Lung Morphology ◽  
Cell Proliferation And Differentiation ◽  
Hyperoxic Exposure ◽  
Proliferation And Differentiation

Postnatal lung development requires proliferation and differentiation of specific cell types at precise times to promote proper alveolar formation. Hyperoxic exposure can disrupt alveolarization by inhibiting cell growth; however, it is not fully understood how this is mediated. The transcription factor CCAAT/enhancer binding protein-α (C/EBPα) is highly expressed in the lung and plays a role in cell proliferation and differentiation in many tissues. After 72 h of hyperoxia, C/EBPα expression was significantly enhanced in the lungs of newborn mice. The increased C/EBPα protein was predominantly located in alveolar type II cells. Silencing of C/EBPα with a transpulmonary injection of C/EBPα small interfering RNA (siRNA) prior to hyperoxic exposure reduced expression of markers of type I cell and differentiation typically observed after hyperoxia but did not rescue the altered lung morphology at 72 h. Nevertheless, when C/EBPα hyperoxia-exposed siRNA-injected mice were allowed to recover for 2 wk in room air, lung epithelial cell proliferation was increased and lung morphology was restored compared with hyperoxia-exposed control siRNA-injected mice. These data suggest that C/EBPα is an important regulator of postnatal alveolar epithelial cell proliferation and differentiation during injury and repair.

scholarly journals Au, Pd and maghemite nanofunctionalized hydroxyapatite scaffolds for bone regeneration

2020 ◽  
Vol 7 (5) ◽  
pp. 461-469
Author(s):  
Giovanna Calabrese ◽  
Salvatore Petralia ◽  
Claudia Fabbi ◽  
Stefano Forte ◽  
Domenico Franco ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Gold Nanorods ◽  
Type I ◽  
Maghemite Nanoparticles ◽  
Cell Growth Inhibition ◽  
Cytotoxic Effects ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
A Cell ◽  
Calcium Deposits

Abstract Nanotechnology plays a key role in the development of innovative scaffolds for bone tissue engineering (BTE) allowing the incorporation of nanomaterials able to improve cell proliferation and differentiation. In this study, Mg-HA-Coll type I scaffolds (Mg-HA-based scaffolds) were nanofunctionalized with gold nanorods (Au NRs), palladium nanoparticles (Pd NPs) and maghemite nanoparticles (MAG NPs). Nanofunctionalized Mg-HA-based scaffolds (NF-HA-Ss) were tested for their ability to promote both the proliferation and the differentiation of adipose-derived mesenchymal stem cells (hADSCs). Results clearly highlight that MAG nanofunctionalization substantially improves cell proliferation up to 70% compared with the control (Mg-HA-based scaffold), whereas both Au NRs and Pd NPs nanofunctionalization induce a cell growth inhibition of 94% and 89%, respectively. Similar evidences were found for the osteoinductive properties showing relevant calcium deposits (25% higher than the control) for MAG nanofunctionalization, while a decreasing of cell differentiation (20% lower than the control) for both Au NRs and Pd NPs derivatization. These results are in agreement with previous studies that found cytotoxic effects for both Pd NPs and Au NRs. The excellent improvement of both osteoconductivity and osteoinductivity of the MAG NF-HA-S could be attributed to the high intrinsic magnetic field of superparamagnetic MAG NPs. These findings may pave the way for the development of innovative nanostructured scaffolds for BTE.

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