scholarly journals Dental Pulp Stem Cell Recruitment Signals within Injured Dental Pulp Tissue

2016 ◽  
Vol 4 (2) ◽  
pp. 8 ◽  
Author(s):  
Charlotte Rombouts ◽  
Charlotte Jeanneau ◽  
Athina Bakopoulou ◽  
Imad About
Keyword(s):  
Stem Cell ◽  
Dental Pulp ◽  
Pulp Tissue ◽  
Cell Recruitment ◽  
Dental Pulp Tissue ◽  
Dental Pulp Stem Cell ◽  
Recruitment Signals

scholarly journals Modulation of Dental Pulp Stem Cell Odontogenesis in a Tunable PEG-Fibrinogen Hydrogel System

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Qiqi Lu ◽  
Mirali Pandya ◽  
Abdul Jalil Rufaihah ◽  
Vinicius Rosa ◽  
Huei Jinn Tong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dental Pulp ◽  
Cross Linking ◽  
Matrix Stiffness ◽  
Pulp Tissue ◽  
Injectable Hydrogels ◽  
Regenerative Endodontics ◽  
Dental Pulp Tissue ◽  
Dental Pulp Stem Cell ◽  
Hydrogel System

Injectable hydrogels have the great potential for clinical translation of dental pulp regeneration. A recently developed PEG-fibrinogen (PF) hydrogel, which comprises a bioactive fibrinogen backbone conjugated to polyethylene glycol (PEG) side chains, can be cross-linked after injection by photopolymerization. The objective of this study was to investigate the use of this hydrogel, which allows tuning of its mechanical properties, as a scaffold for dental pulp tissue engineering. The cross-linking degree of PF hydrogels could be controlled by varying the amounts of PEG-diacrylate (PEG-DA) cross-linker. PF hydrogels are generally cytocompatible with the encapsulated dental pulp stem cells (DPSCs), yielding >85% cell viability in all hydrogels. It was found that the cell morphology of encapsulated DPSCs, odontogenic gene expression, and mineralization were strongly modulated by the hydrogel cross-linking degree and matrix stiffness. Notably, DPSCs cultured within the highest cross-linked hydrogel remained mostly rounded in aggregates and demonstrated the greatest enhancement in odontogenic gene expression. Consistently, the highest degree of mineralization was observed in the highest cross-linked hydrogel. Collectively, our results indicate that PF hydrogels can be used as a scaffold for DPSCs and offers the possibility of influencing DPSCs in ways that may be beneficial for applications in regenerative endodontics.

scholarly journals Dental Pulp Tissue Regeneration Using Dental Pulp Stem Cells Isolated and Expanded in Human Serum

2017 ◽  
Vol 43 (4) ◽  
pp. 568-574 ◽  
Author(s):  
Evandro Piva ◽  
Susan A. Tarlé ◽  
Jacques E. Nör ◽  
Duohong Zou ◽  
Elizabeth Hatfield ◽  
...  
Keyword(s):  
Stem Cells ◽  
Human Serum ◽  
Tissue Regeneration ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Pulp Tissue ◽  
Dental Pulp Tissue ◽  
Pulp Tissue Regeneration
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