scholarly journals PAOT-Liquid® Technology: An Easy Electrochemical Method for Evaluating Antioxidant Capacity of Wines

Diseases ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 10 ◽  
Author(s):  
Joël Pincemail ◽  
Mouna-Messaouda Kaci ◽  
Claire Kevers ◽  
Jessica Tabart ◽  
Raymond Ebabe Elle ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Polyphenols ◽  
Polyphenol Compounds ◽  
Protective Properties ◽  
Commercial Market ◽  
Total Antioxidant ◽  
Wine Polyphenols ◽  
Food Matrices ◽  
Highly Correlated

Polyphenol compounds present in high quantity in wines are well-known to have potent cardio-protective properties through several biological mechanisms including antioxidant activity [1]. A large number of methods have been developed for evaluating the antioxidant capacity of food matrices. Most of them have, however, the disadvantage of being time consuming and require specific analytical protocols and devices. In the present study, we present the electrochemical PAOT (Pouvoir Antioxydant Total)-Liquid® Technology which can be easily used by winemakers for evaluating antioxidant activity of wine during all steps of making process. The methodology is based on the measurement of electric potential variation resulting from chemical reactions between wine polyphenols and a free radical mediator M• as source of oxidants. Total antioxidant activity as estimated by the PAOT-Liquid® activity was 6.8 fold higher in red wines (n = 14) when compared to rosé (n = 3) and white (n = 3) wines bought in a commercial market. Moreover, PAOT-Liquid® activity was highly correlated with total polyphenols content (TPC) of all wines (r = 0.9540, p < 0.0001) and the classical DPPH (2,2-diphenyl-1-picryhydrazyl) assay which is often used for evaluating antioxidant capacity of food matrices (r = 0.9102, p < 0.0001).

EXTRACTION OPTIMIZATION AND ANTIOXIDANT ACTIVITY OF PHENOLIC COMPOUNDS FROM AVOCADO PEEL

2019 ◽  
pp. 49-59
Author(s):  
Nu Linh Giang Ton ◽  
Thi Hoai Nguyen ◽  
Quoc Hung Vo
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Antioxidant Capacity ◽  
Response Surface ◽  
Total Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Quadratic Model ◽  
Total Phenolic ◽  
Total Antioxidant

Avocado peel has been considered as a potential source of natural antioxidants in which phenolics are among the most important compounds. Therefore, this study aims to optimize the extraction process of phenolics using response surface methodology and evaluate the corresponding antioxidant activity. From the quadratic model, the optimal condition was determined including the ethanol concentration 54.55% (v/v), the solvent/solute ratio 71.82/1 (mL/g), temperature 53.03 oC and extraction time 99.09 min. The total phenolic content and the total antioxidant capacity at this condition with minor modifications were 26,74 ± 0,04 (mg GAE/g DW) and 188.06 ± 1.41 (mg AAE/g DW), respectively. The significant correlation between total phenolic content and total antioxidant capacity was also confirmed. Key words: response surface methodology, central composite rotatable design, total phenolic content, total antioxidant capacity, avocado peel

scholarly journals Anti-urease, total phenolic content and antioxidant activities of some Inula l. (Asteraceae) taxa in Turkey

Genetika ◽  
2020 ◽  
Vol 52 (3) ◽  
pp. 825-834
Author(s):  
Emre Sevindik ◽  
Sinem Aydin ◽  
Mehmet Paksoy ◽  
Bahar Sokmen
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Soxhlet Extraction ◽  
Total Phenolic ◽  
Food Industries ◽  
Total Antioxidant ◽  
Ethanol Extracts

The genus Inula belongs to family Asteraceae and it is medicinally important because it possesses cytotoxic, anticancer, hepatoprotective, antibacterial and anti-inflammatory properties. The aim of this research was to explore antioxidant and anti-urease activities of fifteen Inula taxa in Turkey. Ethanol extracts of Inula taxa were obtained by Soxhlet extraction. Antioxidant activity was determined through total antioxidant activity, total phenolic content and copper reducing antioxidant capacity (CUPRAC) analyses. The highest total phenolic content and total antioxidant capacity were found in I. orientalis (158.54?0.002 ?g GAE/mL) and I. macrocephala (189.16?0.002 ?g AAE/mL). CUPRAC activity increases with the increasing concentration of the extracts and the standard. Moreover, all the tested plants exhibited better anti-urease activity than standard thiourea except for I. anatolica and I. thapsoides subsp. thapsoides. Results obtained from this study demonstrate that Inula species might be utilized as a probable natural source in the pharmaceutical and food industries but further and more specific studies are needed to determine active antioxidative and anti-urease compounds in the extracts.

scholarly journals PHYTOCHEMICAL CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF THE NORTHERN MOROCCAN SPECIES: WITHANIA FRUTESCENS L.

2019 ◽  
pp. 276-279 ◽  
Author(s):  
ABDELFATTAH EL MOUSSAOUI ◽  
FATIMA ZAHRA JAWHARI ◽  
DALILA BOUSTA ◽  
AMINA BARI
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Mineral Elements ◽  
Free Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Phytochemical Analysis ◽  
Total Polyphenols

Objective: In this study, we were interested in qualitative, quantitative phytochemical characterization and evaluation of the antioxidant capacity of the total extracts of a plant from northern Morocco, the species selected for this study is Withania frutescens. Materials and Methods: Analysis of mineral elements by inductive coupling plasma-atomic absorption spectroscopy (ICP-AES), phytochemical screening, polyphenol and tannin assays, evaluation of antioxidant activity by the 1,2-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method and reducing power. Results: The plant contains alkaloids, saponins, tannins, mucilages, and coumarins. It has a relatively high content of total polyphenols and tannins of 19.53±0.018 μg genetic generalized epilepsy/mg MS and 6.258±0.062 μg Eqcat/mg MS, respectively. The analysis of mineral elements by ICP-AES shows that our species is rich in mineral elements which are calcium, magnesium, and sodium, and it is devoid of metallic elements such as nickel, lead, cadmium, and cobalt. The evaluation of antioxidant activity by the DPPH free radical scavenging method shows that the half maximal inhibitory concentration of the tested extracts has an antiradical activity of about 0.056±0.008 μg/ml for the ethanol extract and 0.213±0.004 μg/ml for the methanol extract compared to the butylated hydroxytoluene value of 0.009±0.0004 μg/ml which was used as a reference. The reducing capacity test shows that methanolic extract has a high antioxidant capacity (0.213±0.006) compared to ethanolic extract (0.043±0.004) but remains low compared to ascorbic acid (0.003±0.0004) which was used as reference. Conclusion: Phytochemical analysis of W. frutescens shows that this plant is rich in high quantities of alkaloids, saponins, mucilage, tannins, and coumarins. It contains an average amount of total polyphenols and tannins that confer significant antioxidant activity to the plant studied.

scholarly journals Evaluation of Antioxidant and Antibacterial Properties of Magnolia champaca L (Magnoliaceae) Stem Bark Extract

2020 ◽  
Vol 23 (2) ◽  
pp. 96-102
Author(s):  
Md Mahadi Hasan ◽  
Masuma Akter ◽  
Md Ekramul Islam ◽  
Md Aziz Abdur Rahman ◽  
Mst Shahnaj Parvin
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antibacterial Properties ◽  
Reducing Power ◽  
Stem Bark ◽  
Total Antioxidant ◽  
Pharmacologically Active

The current study was designated to explore the antioxidant and antibacterial properties of the methanolic extract of Magnolia champaca stem bark and its different fractions. Antioxidant activity was assessed using total antioxidant capacity, ferric reducing power, DPPH, hydroxyl and hydrogen peroxide scavenging assay. Antibacterial activity was evaluated against five gram positive and five gram negative bacteria using disc diffusion assay method. Among the different fractions, chloroform fraction (CHF) and ethyl acetate fraction (EAF) showed the highest antioxidant activity whereas aqueous fraction (AQF) showed lowest activity in DPPH radical scavenging assay with IC50 of 12.12, 22.41 and 55.16 μg/ml, respectively. Both of the extracts CHF and EAF also exhibited highest total antioxidant capacity, ferric reducing power and hydrogen peroxide scavenging activity with concentration dependent manner when compared to standard BHT. Moderate to potent antibacterial activity was observed against all tested organisms compared to standard azithromycin. The results from the present study revealed that the different fractions of stem bark of M. champaca specially CHF and EAF possess antioxidant and antibacterial property which support its use in traditional medicine and suggesting that the plant may be further investigated to discover its pharmacologically active natural products. Bangladesh Pharmaceutical Journal 23(2): 96-102, 2020

scholarly journals Relations between polyphenols content and antioxidant activity in vine grapes and leaves

2009 ◽  
Vol 26 (Special Issue) ◽  
pp. S25-S32 ◽  
Author(s):  
J. Balík ◽  
M. Kyseláková ◽  
N. Vrchotová ◽  
J. Tříska ◽  
M. Kumšta ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Plasmopara Viticola ◽  
Total Polyphenols ◽  
Antioxidant Power ◽  
Grape Berries ◽  
Caftaric Acid ◽  
Lateral Shoots ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power ◽  
Botryotinia Fuckeliana

The occurrence and content of some polyphenols and the antioxidant activity of compounds present in grape berries, stems and leaves of <i>Vitis vinifera</i> L. were evaluated. Three white and three blue varieties of grapevine were investigated. The contents were determined of <i>trans</i>-resveratrol, <i>trans</i>-piceid, caftaric acid, tryptophan, catechin, epicatechin, total polyphenols, and flavanols, both in healthy material and in the samples of the plant material infested with microorganisms (<i>Botryotinia fuckeliana</i> Whetzel anamorph <i>Botrytis cinerea</i> Pers.; Uncinula necator (Schw.) Burr; <i>Plasmopara viticola</i> (Berk. & M.A. Curtis) Berl & De Toni). The antioxidant activity of the extracts obtained was determined by different methods: FRAP (Ferric Reducing Antioxidant Power), DPPH (2,2-diphenyl-1-picrylhydrazyl radical) and TAC-PCL (Total Antioxidant Capacity of Photochemiluminescence). The content of <i>trans</i>-resveratrol varied between 0.3–2.3 mg/kg and 0.7–12.1 mg/kg in non-infested and infested grape berries, respectively. The content of <i>trans</i>-piceid between 0.6–2.9 mg/kg and 1.5–6.3 mg/kg in non-infested and infested grape berries, respectively. The content of trans-resveratrol varied between 2.5–10.3 mg/kg and 3.7–20.9 mg/kg in healthy and in infected leaves, respectively. The content <i>trans</i>-piceid varied between 11.3–58.4 mg/kg and 18.5–60.9 mg/kg in the healthy and in the infected leaves, respectively. The highest content of trans-resveratrol was found in stems (16.3–276.3 mg/kg). In young lateral shoots, the highest levels of <i>trans</i>-piceid (12.6–99.7 mg/kg) and caftaric acid (474–2257 mg/kg) were determined. The infested grape berries showed a higher antioxidant activity, which was most closely correlated with the content of total polyphenols (correlation coefficient = 0.8336–0.9952).

scholarly journals Chemical Characterization and Antioxidant Activity of Cedrus atlantica Manetti Tar (Atlas Cedar Tar)

2020 ◽  
Vol 8 (2) ◽  
pp. 244-255
Author(s):  
Inssaf Skanderi ◽  
Ourida Chouitah
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Condensed Tannins ◽  
Chemical Characterization ◽  
Antioxidant Power ◽  
Cedrus Atlantica ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power ◽  
Total Polyphenolic Content

Cedrus atlantica Manetti Tar was analyzed by gas chromatography coupled to mass spectrometry, it was subjected to analyzes to know their total polyphenolic and condensed tannins contents, also their Ferric-reducing antioxidant power and Total antioxidant capacity. Chemical characterization identified 88 constituents where Himachalene and α-atlantone isomers (14.51 % - 4.07 %), Calacorene (3.52 %) and ar-Turmerone 3.35 %, were the major components, the total polyphenolic content and condensed tannins contents were 57.15 ± 0.15 milligrams equivalent of gallic acid /g tar and 4.41 ± 0.05 milligrams equivalent of catechin /g tar respectively .This extract showed remarkable Ferric-reducing antioxidant power with effective concentration equal to 50 ± 0.075 mg /mL ± 0,00028 and total antioxidant capacity equal to 262.75 mg equivalents of ascorbic acid /g tar ± 14,43. The experimental results indicated that our tar has promotive antioxidant activity.

scholarly journals Antioxidant Capacity and Polyphenolic Compounds of Blackberries Produced in Different Climates

HortScience ◽  
2019 ◽  
Vol 54 (12) ◽  
pp. 2209-2213 ◽  
Author(s):  
Camila P. Croge ◽  
Francine L. Cuquel ◽  
Paula T.M. Pintro ◽  
Luiz A. Biasi ◽  
Claudine M. De Bona
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Abiotic Factors ◽  
Ascorbic Acid Content ◽  
Climatic Conditions ◽  
Total Polyphenols ◽  
Chemical Structures ◽  
Varied Chemical ◽  
Monomeric Anthocyanins

Antioxidants are compounds with varied chemical structures that are affected by biotic and abiotic factors. The objective of this study was to characterize and compare bioactive compounds and the antioxidant capacity of fruit from four blackberry cultivars produced under different climatic conditions. Ascorbic acid content, total polyphenols, flavonoids, monomeric anthocyanins and antioxidant activity of the fruit were evaluated, and high levels of bioactive compounds as well as antioxidant activity were observed regardless of the cultivar or growing location. The results showed that bioactive production is affected by the cultivar and environment. Furthermore, the antioxidant potential of the blackberry fruit depends on the total phenolics and anthocyanin.

scholarly journals Phytochemical Diversity in Rhizomes of Three Reynoutria Species and their Antioxidant Activity Correlations Elucidated by LC-ESI-MS/MS Analysis

2019 ◽  
Author(s):  
Izabela Nawrot-Hadzik ◽  
Sylwester Ślusarczyk ◽  
Sebastian Granica ◽  
Jakub Hadzik ◽  
Adam Matkowski
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Therapeutic Effects ◽  
Similar Species ◽  
Reynoutria Japonica ◽  
Esi Ms ◽  
Total Antioxidant ◽  
First Time

One of the richest natural sources of resveratrol - the rhizome of Reynoutria japonica in East Asia is a well-known traditional herb (Hu zhang) used in various inflammatory diseases, infections, skin diseases, scald, hyperlipidemia. Although, it has been recently included in the European Pharmacopoeia, still in Europe is an untapped resource. Some of the therapeutic effects are likely to be influenced by its antioxidant properties and this in turn is frequently associated with a high stilbene content. However, some literatures suggested that other compounds than stilbenes may add to the total antioxidant capacity. Hence, the aim of this research was to examine rhizomes of R. japonica and less studied, morphologically similar species, R. sachalinensis and R. x bohemica for their phytochemical composition and antioxidant activity and to clarify the relationship between the antioxidant activity and compounds by statistical methods. HPLC/UV/ESI-MS studies of three Reynoutria species revealed 171 compounds comprising stilbenes, carbohydrates, procyanidins, flavan-3-ols, anthraquinones, phenylpropanoids, lignin oligomers, hydroxycinnamic acids, naphthalenes and their derivatives. Our studies confirmed the presence of procyanidins with high degree of polymerization, up to decamers in the rhizomes of R. japonica and brings new data on the presence of these compounds in other Reynoutria species. A procyanidin trimer digallate was described for the first time in the studied plants. Moreover, we suggested a presence of new for these species, dianthrone glycosides and previously unrecorded phenylpropanoid disaccharide esters and hydroxycinnamic acid derivatives, mainly in R. sachalinensis. Furthemore, compounds tentatively annotated as lignin oligomers were observed for the first time in studied species. The rhizomes of all Reynoutria species exhibited strong antioxidant activity. Statistical analysis demonstrated that proanthocyanidins should be considered as important contributors to the total antioxidant capacity.

scholarly journals Correlation of Total Polyphenolic Content with Antioxidant Activity of Hydromethanolic Extract and Their Fractions of the Salvia officinalis Leaves from Different Regions of Morocco

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Zakaria Khiya ◽  
Yassine Oualcadi ◽  
Abderrahmane Gamar ◽  
Fatima Berrekhis ◽  
Touria Zair ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cyclic Voltammetry ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Condensed Tannins ◽  
Salvia Officinalis ◽  
Total Phenolic ◽  
Total Antioxidant ◽  
Pharmaceutical Industries

The purpose of this study is to determine the total content of phenols, flavonoids, and condensed tannins, as well as on the antioxidant activity of the extract, and their fractions were measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), phosphomolybdate reduction (or total antioxidant capacity), and cyclic voltammetry (CV). The hydromethanolic extract of Salvia officinalis showed the highest values of total phenolic (176 mgGAE/g of extract) and condensed tannins (162.53 mgEC/g of extract) from the Boulemane and Khenifra regions, respectively. The results showed that the best DPPH assay was found in the ethyl acetate fraction of Salvia officinalis leaves of the Boulemane region (IC50 = 0.002 mg/ml). For the ethyl acetate and butanolic fractions of Salvia officinalis leaves, those collected from different regions have a better reducing capacity (EC50 = 0.021 mg/ml, respectively). For the total antioxidant capacity, the best activity was found in the aqueous fraction of Salvia officinalis leaves of the Boulemane region (108 mgGAE/g of extract). By the cyclic voltammetry method, hydromethanolic extract of Salvia officinalis leaves from the Boulemane region showed an important result (288.8 mgGAE/g). There was a positive correlation between total phenol content (TPC), condensed tannin content (TCT), and total antioxidant capacity (TAC) (r = 0.932, r = 0.896, respectively). The main compounds that have been identified in the hydromethanolic extract of Salvia officinalis are ascorbic acid, gallic acid, 4-hydroxybenzoic acid, tannic acid, and rutin. Due to their antioxidant property, the leaf extracts from Salvia officinalis are used as natural preservative ingredients in food and/or pharmaceutical industries.

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