scholarly journals Comparison of Humoral and Cellular CMV Immunity in Patients Awaiting Kidney Transplantation

Diagnostics ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1688
Author(s):  
Monika Lindemann ◽  
Benjamin Wilde ◽  
Justa Friebus-Kardash ◽  
Anja Gäckler ◽  
Oliver Witzke ◽  
...  
Keyword(s):  
Kidney Transplantation ◽  
T Cell ◽  
Immunosuppressive Therapy ◽  
Positive Control ◽  
T Cell Immunity ◽  
Median Response ◽  
Humoral And Cellular Immunity ◽  
Cell Immunity ◽  
Increased Risk ◽  
Cmv Dnaemia

Chronic kidney disease may alter antiviral T cell immunity. In the current study, we assessed in 63 patients prior to kidney transplantation how humoral and cellular immunity against cytomegalovirus (CMV) correlated using an interferon (IFN)-γ ELISpot (T-Track® CMV, Mikrogen, Neuried, Germany). The cohort comprised 24 patients with negative and 39 with positive CMV IgG. Whereas none of the patients with negative CMV IgG showed detectable responses to the T-Track® CMV, 26 out of 39 patients with positive CMV IgG had positive ELISpot responses. The median response to CMV pp65 in the CMV seronegative group was 0 spot forming units (SFU) per 200,000 PBMC (range 0–1) and in the seropositive group 43 SFU (range 0–750). Thus, 13 out of 39 patients with positive CMV serostatus (33%) had undetectable T cell immunity and may be at an increased risk of CMV reactivation. CMV pp65-specific ELISpot responses were 29.3-fold higher in seropositive patients with vs. without dialysis and 5.6-fold higher in patients with vs. without immunosuppressive therapy, but patients with dialysis and immunosuppressive therapy showed, as expected, lower responses to phytohemagglutinin, the positive control. This finding may be caused by (subclinical) CMV-DNAemia and a “booster” of CMV-specific T cells.

scholarly journals 2508. Absolute Lymphocyte Count and Adenovirus-Specific CD8+ T-cell Immunity as Immunological Predictors of Severe Adenovirus Disease After Kidney Transplantation

2018 ◽  
Vol 5 (suppl_1) ◽  
pp. S754-S754
Author(s):  
Jackrapong Bruminhent ◽  
Nopporn Apiwattanakul ◽  
Subencha Pinsai ◽  
Charat Thongprayoon ◽  
Suradej Hongeng ◽  
...  
Keyword(s):  
Kidney Transplantation ◽  
T Cell ◽  
Lymphocyte Count ◽  
Absolute Lymphocyte Count ◽  
Cd8 T Cell ◽  
T Cell Immunity ◽  
Cell Immunity

scholarly journals The Effect of Induction Therapy on Established CMV Specific T Cell Immunity in Living Donor Kidney Transplantation

2018 ◽  
pp. 251-260 ◽  
Author(s):  
L. STRANAVOVA ◽  
P. HRUBA ◽  
E. GIRMANOVA ◽  
I. TYCOVA ◽  
A. SLAVCEV ◽  
...  
Keyword(s):  
Kidney Transplantation ◽  
T Cell ◽  
Living Donor ◽  
T Cell Immunity ◽  
Transplant Recipients ◽  
Donor Kidney ◽  
Cell Immunity ◽  
Dialysis Vintage ◽  
Cmv Prophylaxis ◽  
Living Donor Kidney

Cytomegalovirus (CMV) infection influences both short and long term outcomes in immunosuppressed organ transplant recipients. The aim of this study was to evaluate the effect of different induction immunosuppression regimens on CMV specific T cell response in patients with already established CMV immunity. In 24 seropositive living donor kidney recipients, the frequency of CMV specific T cells was determined by ELISPOT (Enzyme-Linked ImmunoSpot) assay prior and 6 months after transplantation. Recipients’ peripheral blood mononuclear cells were stimulated with immediate-early (IE1) and phosphoprotein 65 (pp65) CMV-derived peptide pools and the number of cells producing interferon gamma (IFN-γ) was assessed. Patients received quadruple immunosuppression based either on depletive rabbit antithymocyte globulin (rATG) or non-depletive basiliximab induction and tacrolimus/mycophenolate mofetil/steroids. Patients with rATG induction received valgancyclovir prophylaxis. No effects of different induction agents on CMV specific T cell immunity were found at sixth month after kidney transplantation. There were no associations among dialysis vintage, pretransplant CMV specific T cell immunity, and later CMV DNAemia. Similarly, no effect of CMV prophylaxis on CMV specific T cell immunity was revealed. This study shows no effect of posttransplant immunosuppression on CMV specific T cell immunity in living donor kidney transplant recipients with CMV immunity already established, regardless of lymphocyte depletion and CMV prophylaxis.

scholarly journals Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals

2021 ◽  
Author(s):  
Carmen Camara ◽  
Daniel Lozano-Ojalvo ◽  
Eduardo Lopez-Granados ◽  
Estela Paz-Artal ◽  
Marjorie Pion ◽  
...  
Keyword(s):  
T Cell ◽  
Severe Acute Respiratory Syndrome ◽  
Cellular Immunity ◽  
Rapid Development ◽  
Vaccine Dose ◽  
T Cell Immunity ◽  
Current Standard ◽  
Standard Regimen ◽  
Humoral And Cellular Immunity ◽  
Cell Immunity

The rapid development and deployment of mRNA-based vaccines against the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) led to the design of accelerated vaccination schedules that have been extremely effective in naive individuals. While a two-dose immunization regimen with the BNT162b2 vaccine has been demonstrated to provide a 95% efficacy in naive individuals, the effects of the second vaccine dose in individuals who have previously recovered from natural SARS-CoV-2 infection has been questioned. Here we characterized SARS-CoV-2 spike-specific humoral and cellular immunity in naive and previously infected individuals during full BNT162b2 vaccination. Our results demonstrate that the second dose increases both the humoral and cellular immunity in naive individuals. On the contrary, the second BNT162b2 vaccine dose results in a reduction of cellular immunity in COVID-19 recovered individuals, which suggests that a second dose, according to the current standard regimen of vaccination, may be not necessary in individuals previously infected with SARS-CoV-2.

scholarly journals Dynamic Clonal Hematopoiesis and Functional T-cell Immunity in a Super-centenarian

2019 ◽  
Author(s):  
Erik B. van den Akker ◽  
Stavros Makrodimitris ◽  
Marc Hulsman ◽  
Martijn H. Brugman ◽  
Tanja Nikolic ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Immunity ◽  
List Type ◽  
Hematopoietic Stem ◽  
Blood Samples ◽  
Clonal Hematopoiesis ◽  
Cell Immunity ◽  
Increased Risk

AbstractThe aged hematopoietic system is characterized by decreased immuno-competence and by a reduced number of hematopoietic stem cells (HSCs) that actively generates new blood cell (age-related clonal hematopoiesis, ARCH). While both aspects are commonly associated with an increased risk of aging-related diseases, it is currently unknown to what extent these aspects co-occur during exceptional longevity. Here, we investigated these aspects in blood cells of an immuno-hematopoietically normal female who reached 111 years. Blood samples were collected across a 9-year period at ages 103, 110 and 111 years. We applied several genetic sequencing approaches to investigate clonality in peripheral blood samples and sorted cell subsets. Immuno-competence was characterized using flow cytometry, T-cell receptor excision circle (TREC) assays, and in vitro proliferation assays. We identified a single DNMT3A-mutated HSC clone with a complex subclonal architecture and observed ongoing subclonal dynamics within the 9-year timeframe of our sampling. The mutated HSC generated 78-87% myeloid cells, 6-7% of the B-cells, 6% of CD8+ T-cells, and notably 22% of the CD4+ T-cells. Intriguingly, we found that T-cells were capable of robust proliferation when challenged in vitro. Moreover, we observed a surprisingly high TREC content, indicative of recent generation of naive T-cells. Concluding, we observed long-term stability of extreme ARCH with ongoing clonal dynamics combined with functional T-cell immunity. Our results indicate that extreme ARCH does not compromise immuno-competence and that a clonally expanded CD4+ T-cell subset may serve as a potential hallmark of the supercentenarian immune system.Key pointsLongitudinal blood sampling from a female aged 103-111 revealed a dynamic clonal hematopoiesis contributing to myeloid and lymphoid subsetsDespite the highly advanced age and extreme clonal hematopoiesis we observed functional T-cell immunity

IL-7 and IL-15: Biology and Roles in T-Cell Immunity in Health and Disease

2008 ◽  
Vol 28 (4) ◽  
pp. 325-339 ◽  
Author(s):  
Hang-Rae Kim ◽  
Kyung-A Hwang ◽  
Sung-Hwan Park ◽  
Insoo Kang
Keyword(s):  
T Cell ◽  
T Cell Immunity ◽  
Cell Immunity ◽  
Health And Disease
Sign in / Sign up

Export Citation Format

Share Document