scholarly journals Raman Spectroscopy for Assessment of Hard Dental Tissues in Periodontitis Treatment

Diagnostics ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1595
Author(s):  
Elena V. Timchenko ◽  
Irina V. Bazhutova ◽  
Oleg О. Frolov ◽  
Larisa Т. Volova ◽  
Pavel Е. Timchenko
Keyword(s):  
Raman Spectroscopy ◽  
Dental Plaque ◽  
Tissue Repair ◽  
Plaque Formation ◽  
Discriminant Model ◽  
Dental Tissues ◽  
Spectral Changes ◽  
Dental Procedures ◽  
Before And After ◽  
Hard Dental Tissues

The objective of this work was to use Raman spectroscopy to assess hard dental tissues after professional oral hygiene treatment and curettage. Spectral changes were identified, and the discriminant model of the specific changes of intensity of the Raman lines (i.e., of dentin, cementum, and enamel), before and after the dental procedures, was developed. This model showed that 6 weeks after the procedures, the hard dental tissues did not have differences and, thus, provided similar conditions for bio-film and dental plaque formation, tissue repair, and new attachment to the surface of the root.

scholarly journals Erosive Effect of Acidic Beverages On Dental Filling Materials Surface (In Vitro Study)

2021 ◽  
Author(s):  
Yuliya Morozova ◽  
Barbora Novotná ◽  
Pavel Holík ◽  
Iva Voborná ◽  
Jana Zapletalová
Keyword(s):  
In Vitro Study ◽  
Dental Amalgam ◽  
Vitro Study ◽  
Dental Tissues ◽  
Dental Filling ◽  
Filling Materials ◽  
Before And After ◽  
Acidic Conditions ◽  
Hard Dental Tissues

Abstract Erosive tooth wear, that is characterized like irreversible loss of hard dental tissues due to influence of external and internal acids of nonbacterial origin, is one of the most common damage of a tooth surface. Erosive factors also affect the surface of dental reconstruction materials that ideally should be resistant against them. Dental filling materials designed for this purpose must be able to withstand these effects in a comparable or even better way than enamel. In our in vitro study we investigated using the nanoindentation the hardness of currently available dental filling materials (dental composite resin, self curing and dual cures glassionomer cement, dental amalgam) before and after erosive attacks (exposure to Coca Cola for 5 min and 14 days) and consequently compare it with them of human dental enamel. Furtermore we analised the surface topography of the samples before and after acidic exposure by confocal laser scanning microscope. In our experiments the filling materials were affected by the acidic environment only minimally in comparison with hard dental tissues. We confirmed the fact that dental amalgam is still the most mechanically and chemically resistant filling material. Both GIC materials showed the worst resistance against acidic conditions and higher surface roughness even before exposure. We also declared excellent withstanding of resin composite materials against acidic conditions that was even better than natural enamel. Furthermore by CLSM was detected almost unaltered surface of these filling materials. This fact as well as adhesive properties of resin composites allow to assume that this material could be the most suitable material for dental erosion reconstruction.

scholarly journals Improving the effectiveness of tooth preparation by using alternative methods

2020 ◽  
pp. 4-9
Author(s):  
N. O. Gevkaliuk
Keyword(s):  
Morphological Characteristics ◽  
Alternative Methods ◽  
Maximum Height ◽  
Preparation Methods ◽  
Dental Tissues ◽  
Dental Procedures ◽  
Effective Removal ◽  
Rotary Instruments ◽  
Invasive Method ◽  
Hard Dental Tissues

Summary. Preparation of hard tissues of teeth is an integral stage of the invasive method of treating caries and is a complex effect of various factors that make it possible to remove tissues affected by this process and the formation of a cavity of arbitrary shape, based on the principles of gentle preparation, which consists in the most effective removal of demineralized with the preservation of intact tissues. The aim of the study – determination of enamel and dentin surface aspects when using ultrasonic type of odontopreparation compared with the preparation of teeth with rotary instruments. Materials and Methods. Intact retinal teeth of the chewing group, removed by surgical indications, were used as material for the morphological study. After special preparation, the teeth were dissected using ultrasound with the help of special nozzles and in the classical way using rotary instruments, subject to the protocols of manipulations. Subsequently, histological preparations of these teeth were made and examined by light microscopy at magnification x 400. Results and Discussion. In the case of ultrasound preparation, single invaginations were visualized on the surface, which were dissociated by prongs and equal sections of dentin. The tips of dentin prongs are smoothed, the taper is about 800. The maximum height of the prong does not exceed 1.3 microns. In the area of the bottom and walls of the cavity all over the existing lubricated layer in the form of a thin structureless weakly basophilic line, smaller in area compared to the same in the cavity, prepared with rotary tools. At preparation with rotary instruments, small invaginations alternating with pronounced prongs were observed at the bottom and walls. Flat levels were not visualized. The taper of the prongs is about 600 the maximum height of the prong is 2.4 microns. In the region of the bottom and walls of the cavity along the boundary of the preparation, a structured layer is smeared in the form of a thin sharply basophilic line. Conclusions. The comparative characterization of dental preparation methods makes it possible to recommend an ultrasound method as a method of choice for a specific contingent of individuals, since it not only provides painless preparation and minimizes stress during dental procedures, but is also gentler on the morphological characteristics of hard dental tissues.

scholarly journals Possibility of Raman spectroscopy method use for evaluation of periodontitis-affected tooth enamel before and after open curettage

2021 ◽  
Vol 2103 (1) ◽  
pp. 012055
Author(s):  
P E Timchenko ◽  
E V Timchenko ◽  
I V Bazhutova ◽  
O O Frolov ◽  
L T Volova ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Mineral Composition ◽  
Raman Spectra ◽  
Evaluation Method ◽  
Tooth Enamel ◽  
Organic Matrix ◽  
Chemometric Analysis ◽  
Spectroscopy Method ◽  
Spectral Changes ◽  
Before And After

Abstract The results of the research of enamel of periodontitis-affected teeth before and after open curettage are presented in the work. The Raman spectroscopy method was used as an evaluation method of curettage influence on tooth enamel. Chemometric analysis of the Raman spectra of tooth enamel before and after curettage was made. Spectral changes of tooth enamel were detected. It was found that after the procedure of open curettage spectral changes occur in tooth enamel related to the changes in mineral composition and organic matrix forming.

scholarly journals MORPHOLOGICAL STRUCTURE OF ENAMY, DENTINE OF TEETH AND COMPOSITE FORMING MATERIALS IN VITRO

2018 ◽  
Vol 22 (4) ◽  
pp. 176-179
Author(s):  
V. I Kolodkina ◽  
Armenak Valer'evich Arutyunov
Keyword(s):  
Morphological Structure ◽  
Adhesive Bond ◽  
Point Of View ◽  
Dental Tissues ◽  
Direct Exposure ◽  
Urgent Task ◽  
Before And After ◽  
Hard Dental Tissues ◽  
General Provision

Solving the problem of increasing the effectiveness of treatment of diseases of hard tooth tissues is an urgent task not only for the dental community of doctors, but for dentistry in general. Provision of edge tightness and high adhesive bond of copolytic material with enamel and dentin to prevent the occurrence of micro-permeability of restoration for toxic agents and subsequent inflammation of pulp is one of the most urgent problems of therapeutic dentistry. Universal microhybrid composites used for sealing all groups of teeth were created in the last century, however, a number of important problems remain unresolved until now. The purpose of this study was to assess the morphological structure of the enamel and dentin of the teeth before and after using restorative sealing materials with and without sealant coating. The data obtained as a result of the research allows us to estimate from the scientific point of view the changes in the micro-morphological structure of enamel and dentin at the points of contact with the composite material, accompanied by a decrease in the microhardness of the dentine on the background of unsatisfactory oral hygiene, increased consumption of soft and carbohydrate-rich foods, salivary capacity, as well as direct exposure of microorganisms and products of their vital activity to hard dental tissues.

scholarly journals Hard Dental Tissues Regeneration—Approaches and Challenges

Materials ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2558
Author(s):  
Mihaela Olaru ◽  
Liliana Sachelarie ◽  
Gabriela Calin
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Growth Factors ◽  
Present Review ◽  
Modern Concept ◽  
Dental Tissues ◽  
Tissue Engineering Approach ◽  
Hard Dental Tissues ◽  
Review Reports

With the development of the modern concept of tissue engineering approach and the discovery of the potential of stem cells in dentistry, the regeneration of hard dental tissues has become a reality and a priority of modern dentistry. The present review reports the recent advances on stem-cell based regeneration strategies for hard dental tissues and analyze the feasibility of stem cells and of growth factors in scaffolds-based or scaffold-free approaches in inducing the regeneration of either the whole tooth or only of its component structures.

The role of sugar-rich diet and salivary proteins in dental plaque formation and oral health

2021 ◽  
Author(s):  
Asma Gasmi Benahmed ◽  
Amin Gasmi ◽  
Maryam Dadar ◽  
Maria Arshad ◽  
Geir Bjørklund
Keyword(s):  
Oral Health ◽  
Dental Plaque ◽  
Plaque Formation ◽  
Salivary Proteins
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