scholarly journals The Role of CX3CL1 and ADAM17 in Pathogenesis of Diffuse Parenchymal Lung Diseases

Diagnostics ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1074
Author(s):  
Jan Urban ◽  
Magda Suchankova ◽  
Martina Ganovska ◽  
Vladimir Leksa ◽  
Frantisek Sandor ◽  
...  
Keyword(s):  
T Cells ◽  
Lung Diseases ◽  
Lung Parenchyma ◽  
Elisa Test ◽  
The Other ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Cytotoxic Lymphocytes ◽  
Diffuse Parenchymal Lung Diseases ◽  
Parenchymal Lung

Fractalkine (CX3CL1) is a unique chemokine that functions as a chemoattractant for effector cytotoxic lymphocytes and macrophages expressing fractalkine receptor CX3CR1. CX3CL1 exists in two forms—a soluble and a membrane-bound form. The soluble CX3CL1 is released from cell membranes by proteolysis by the TNF-α-converting enzyme/disintegrin-like metalloproteinase 17 (TACE/ADAM17) and ADAM10. In this study, we evaluated the diagnostic relevance and potential roles of CX3CL1 and ADAM17 in the pathogenesis of diffuse parenchymal lung diseases (DPLDs) in the human population. The concentration of CX3CL1 and ADAM17 was measured by the enzyme-linked immunosorbent assay (ELISA) test in bronchoalveolar lavage fluids of patients suffering from different DPLDs. The concentration of CX3CL1 was significantly higher in patients suffering from idiopathic pulmonary fibrosis (IPF) and hypersensitivity pneumonitis patients compared to the control group. A significantly higher concentration of CX3CL1 was measured in fibrotic DPLDs compared to non-fibrotic DLPD patients. We found a positive correlation of CX3CL1 levels with the number of CD8+ T cells, and a negative correlation with CD4+ T cells in BALF and diffusion capacity for carbon monoxide. The concentration of ADAM17 was significantly lower in the IPF group compared to the other DPLD groups. We noticed a significantly higher CX3CL1/ADAM17 ratio in the IPF group compared to the other DPLD groups. We suggest that CX3CL1 has a distinctive role in the pathogenesis of DPLDs. The level of CX3CL1 strongly correlates with the severity of lung parenchyma impairment. The results suggest that high values of CX3CL1/ADAM17 could be diagnostic markers for IPF.

Interstitial lung disease

2018 ◽  
Author(s):  
Patrick Davey ◽  
Sherif Gonem ◽  
David Sprigings
Keyword(s):  
Interstitial Lung Disease ◽  
Lung Disease ◽  
Lung Diseases ◽  
Lung Parenchyma ◽  
Interstitial Lung Diseases ◽  
Broad Group ◽  
Diffuse Parenchymal Lung Diseases ◽  
Parenchymal Lung

The interstitial lung diseases, also known as the diffuse or diffuse parenchymal lung diseases, are a broad group of pulmonary disorders which mainly affect the lung parenchyma as opposed to the airways. By convention, infectious and malignant conditions are excluded from this definition. Thus, the interstitial lung diseases comprise a group of conditions characterized by variable degrees of inflammation and fibrosis, centred on the lung interstitium and alveolar airspaces.

scholarly journals EFFECT OF VITAMIN E AND COD LIVER OIL SUPPLEMENT WITH BIVALENT OIL BASED VACCINE OF NEWCASTLE DISEASE AND INFECTIOUS BRONCHITIS DISEASE ON IMMUNE RESPONSE OF THE BROILERS.

2016 ◽  
Vol 47 (3) ◽  
Author(s):  
ABDULWAHID & et al.
Keyword(s):  
Immune Response ◽  
Vitamin E ◽  
Newcastle Disease ◽  
Challenge Test ◽  
Elisa Test ◽  
The Other ◽  
Control Group ◽  
Broiler Chicks ◽  
Cod Liver Oil ◽  
Infectious Bronchitis

This study was aimed to enhance the immune response of the broilers by vitamin E and Cod liver oil supplement with locally inactivated bivalent vaccine of Newcastle disease and Infectious bronchitis viruses. One hundred and twenty broiler chicks Ross 308 were divided to four groups at 30 chicks of each group. The 2nd group was vaccinated with 0.5ml/chick of locally inactivated ND-IB vaccine at 5 days old. The 3rd group was vaccinated with 0.5ml locally ND-IB vaccine and 0.1 ml/chick of VE. The 4th group was vaccinated with 0.5ml of locally ND-IB vaccine and 0.1ml/chick of Cod liver oil, while the first group was left without vaccination as a control group. All groups were vaccinated with attenuated ND-IB at 3 days old and attenuated IBD at 14 days old. The results of ELISA test revealed that the 3rd and 4th groups showed significantly increased (P≤0.05) in Ab titers against ND virus at aged 35 days, also, the 4th group showed significantly (P≤0.05) increased in Ab titers against IB virus at day 35 of age as compared with the other groups. The 4th group showed a significant (P≤0.05) increased in bursal index as compared with the other groups. The results of challenge test revealed that vaccinated groups resist the velogenic ND and IB viruses with relatively high productive range particularly the 4th group as compared with other groups. In conclusion, the VE and Cod liver oil supplement with local bivalent oil based vaccine of ND and IBV may enhance the immune response of the broilers particularly the 4th group which vaccinated with 0.5 ml of local bivalent oil based vaccine of ND-IB vaccine and 0.1 ml Cod liver oil.

scholarly journals Diagnostic approach in parenchymal lung diseases: transbronchial lung biopsy or cryobiopsy?

2020 ◽  
Vol 50 (6) ◽  
pp. 1535-1539
Author(s):  
Ali Kadri ÇIRAK ◽  
Nuran KATGI ◽  
Onur Fevzi ERER ◽  
Pınar ÇİMEN ◽  
Fatma Fevziye TUKSAVUL ◽  
...  
Keyword(s):  
Lung Biopsy ◽  
Lung Diseases ◽  
Interstitial Lung Diseases ◽  
Massive Hemorrhage ◽  
Open Lung Biopsy ◽  
Transbronchial Lung Biopsy ◽  
Diffuse Parenchymal Lung Diseases ◽  
Training And Research ◽  
Transbronchial Lung Cryobiopsy ◽  
Parenchymal Lung

Background/aim: Diagnosis of interstitial lung diseases requires a multidisciplinary approach, and a gold standard for histological diagnosis is open lung biopsy. Transbronchial lung biopsy (TBLB) and in recent years an alternative method, cryobiopsy (TBLC), are used for the diagnosis of parenchymal lung lesions. The aim of this study is to compare the efficacy of concomitant conventional TBLB and TBLC.Materials and methods: A total of 82 patients who underwent TBLC for diagnosis of diffuse parenchymal lung diseases at Dr. Suat Seren Chest Diseases and Surgery Training and Research Hospital between 2015 and 2018 were screened retrospectively and included in the study. Of the patients, 53.7% (n: 44) were male, and 46.4% (n:38) of them were female. The mean age was 58.37 (±9.33) years. First TBLB and then TBLC were performed to all patients in the same session and their diagnostic performances were compared.Results: Although both procedures were done in the same session, 45 patients (54.9%) were diagnosed with TBLB and 75 patients (91.5%) were diagnosed with TBLC (P ˂ 0.001). Hemorrhage was observed in 39 patients (47.6%), but only one had a massive hemorrhage. Pneumothorax was observed in 6 patients (7.3%) and none of them required tube drainage.Conclusion: Transbronchial lung cryobiopsy is a promising technique for the diagnosis of parenchymal lung diseases compared to transbronchial lung biopsy.

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