scholarly journals Gingival Crevicular Placental Alkaline Phosphatase Is an Early Pregnancy Biomarker for Pre-Eclampsia

Diagnostics ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 661
Author(s):  
Alejandra Chaparro ◽  
Maximiliano Monckeberg ◽  
Ornella Realini ◽  
Marcela Hernández ◽  
Fernanda Param ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Alkaline Phosphatase ◽  
Systolic Blood Pressure ◽  
Gingival Crevicular Fluid ◽  
Classification Model ◽  
Study Cohort ◽  
Placental Alkaline Phosphatase ◽  
Predictive Values ◽  
Diagnostic Strategies ◽  
Crevicular Fluid

Early and innovative diagnostic strategies are required to predict the risk of developing pre-eclampsia (PE). The purpose of this study was to evaluate the performance of gingival crevicular fluid (GCF) placental alkaline phosphatase (PLAP) concentrations to correctly classify women at risk of PE. A prospectively collected, retrospectively stratified cohort study was conducted, with 412 pregnant women recruited at 11–14 weeks of gestation. Physical, obstetrical, and periodontal data were recorded. GCF and blood samples were collected for PLAP determination by ELISA assay. A multiple logistic regression classification model was developed, and the classification efficiency of the model was established. Within the study cohort, 4.3% of pregnancies developed PE. GCF-PLAP concentration was 3- to 6-fold higher than in plasma samples. GCF-PLAP concentrations and systolic blood pressure were greater in women who developed PE (p = 0.015 and p < 0.001, respectively). The performance of the multiparametric model that combines GCF-PLAP concentration and the levels of systolic blood pressure (at 11–14 weeks gestation) showed an association of systolic blood pressure and GCF-PLAP concentrations with the likelihood of developing PE (OR:1.07; 95% CI 1.01–1.11; p = 0.004 and OR:1.008, 95% CI 1.000–1.015; p = 0.034, respectively). The model had a sensitivity of 83%, a specificity of 72%, and positive and negative predictive values of 12% and 99%, respectively. The area under the receiver operating characteristic (AUC-ROC) curve was 0.77 and correctly classified 72% of PE pregnancies. In conclusion, the multivariate classification model developed may be of utility as an aid in identifying pre-symptomatic women who subsequently develop PE.

Gingival Crevicular Fluid Alkaline Phosphatase Levels in Postmenopausal Women: Effects of Phase I Periodontal Treatment

2006 ◽  
Vol 77 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Özlem Daltaban ◽  
Işıl Saygun ◽  
Belgin Bal ◽  
Köksal Baloş ◽  
Muhittin Serdar
Keyword(s):  
Alkaline Phosphatase ◽  
Phase I ◽  
Postmenopausal Women ◽  
Gingival Crevicular Fluid ◽  
Periodontal Treatment ◽  
Crevicular Fluid

A new ultrasensitive chemiluminescent assay for the site-specific quantification of alkaline phosphatase in gingival crevicular fluid

1993 ◽  
Vol 28 (4) ◽  
pp. 266-273 ◽  
Author(s):  
I. L. C. Chapple ◽  
J. B. Matthews ◽  
G. H. G. Thorpe ◽  
H. D. Glenwright ◽  
J. M. Smith ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Gingival Crevicular Fluid ◽  
Site Specific ◽  
Crevicular Fluid

scholarly journals Plasma S1P links to hypertension and biomarkers of inflammation, metabolism and cardiovascular disease – findings from a translational investigation

2020 ◽  
Author(s):  
Amra Jujic ◽  
Frank Matthes ◽  
Lotte Vanherle ◽  
Henning Petzka ◽  
Marju Orho-Melander ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Cardiovascular Disease ◽  
Systolic Blood Pressure ◽  
Immune Cell ◽  
Vascular Dysfunction ◽  
Plasma Concentrations ◽  
Human Study ◽  
Experimental Hypertension ◽  
Study Cohort ◽  
Markers Of Inflammation

Recent studies identified sphingosine-1-phosphate (S1P) as an important player in immune cell trafficking and vascular dysfunction contributing to the development and progression of overt hypertension. Although targeting S1P signaling revealed therapeutic potential in different experimental hypertension studies, validations of S1P-blood pressure associations in humans are lacking. In a translational approach, we explored the associations between plasma S1P, quantified using LC-MS, and blood pressure in a family-based study cohort (MOS) study, and in a longitudinally conducted murine hypertension cohort. In MOS, linear multivariate regression analyses showed that plasma S1P associates with increased systolic blood pressure. Study subjects with systolic blood pressure ≥ 140 mmHg presented with significantly higher S1P plasma concentrations compared to subjects with blood pressure ≤ 120 mmHg independent of age and sex. The S1P-blood pressure association was validated in a murine model where plasma S1P increased with systolic blood pressure. In a sub-sample of the human study population, proteomic profiling for markers of inflammation, metabolism and cardiovascular disease was carried out using proximity Extension Assays. Testing S1P associations revealed multiple significant interactions, some of them with marked sex-specificity. Amongst them, interleukin 18, which exerts apparent vascular and immune responses during hypertension and associates to adverse cardiovascular events, strongly correlates with plasma S1P concentrations in females but not males in both humans and mice. In vitro and ex vivo validation of S1P effects on endothelial and monocytic cells of murine or human origin and resistance arteries isolated from mice disclosed augmented expression of different vascular dysfunction and inflammation markers in response to exogenously added S1P. Taken together, our translational findings strongly suggest a link between plasma S1P and systolic blood pressure as well as several inflammation and cardiovascular disease biomarkers in humans, encouraging further studies to investigate S1Ps potential as a therapeutic target in hypertensive disease.

Alkaline phosphatase activity in gingival crevicular fluid during human orthodontic tooth movement

2002 ◽  
Vol 122 (5) ◽  
pp. 548-556 ◽  
Author(s):  
Giuseppe Perinetti ◽  
Michele Paolantonio ◽  
Michele D'Attilio ◽  
Domenico D'Archivio ◽  
Domenico Tripodi ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Orthodontic Tooth Movement ◽  
Crevicular Fluid

Dentition phase and chronological age in relation to gingival crevicular fluid alkaline phosphatase activity in growing subjects

2011 ◽  
Vol 12 (2) ◽  
pp. 100-106 ◽  
Author(s):  
Giuseppe Perinetti ◽  
Tiziano Baccetti ◽  
Bruno Di Leonardo ◽  
Roberto Di Lenarda ◽  
Luca Contardo
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Gingival Crevicular Fluid ◽  
Chronological Age ◽  
Crevicular Fluid

Diagnostic Potential of Host Response Mediators

1993 ◽  
Vol 7 (2) ◽  
pp. 175-181 ◽  
Author(s):  
S. Offenbacher ◽  
J.G. Collins ◽  
P.A. Heasman
Keyword(s):  
Disease Activity ◽  
Host Response ◽  
Gingival Crevicular Fluid ◽  
Diagnostic Tools ◽  
Predictive Values ◽  
Diagnostic Measures ◽  
Diagnostic Potential ◽  
Crevicular Fluid ◽  
Sensitivity Specificity ◽  
Considerable Body

This review summarizes the data which relate the validation and application of host response markers as diagnostic tests forperiodontal diseases. Practical considerations regarding the general application and evaluation of a diagnostic test for periodontal disease are presented. Experiments which have documented the performance of host response markers as diagnostic measures of disease activity in terms of sensitivity, specificity, and predictive values are summarized. Particular emphasis is placed on the diagnostic potential of gingival crevicular fluid (GCF) components. The considerable body of evidence supporting the potential application of GCF-PGE2 levels for predicting episodes of disease progression and reflecting disease activity is summarized and placed into perspective for the development of future diagnostic tools.

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