scholarly journals Usefulness of the IgA and IgG Responses to Macrophage Migration Inhibitory Factor for the Diagnosis of Tuberculosis

Diagnostics ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 991
Author(s):  
Ji Yeon Lee ◽  
Byoung-Jun Kim ◽  
Jee-min Kim ◽  
Junghyun Kim ◽  
Joon-Sung Joh ◽  
...  
Keyword(s):  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Latent Tuberculosis ◽  
Protective Role ◽  
Antibody Responses ◽  
Inhibitory Factor ◽  
Macrophage Migration ◽  
Serological Tests ◽  
Iga Antibody ◽  
Ifn Γ

Serological tests offer the potential in order to improve the diagnosis of tuberculosis (TB). Macrophage migration inhibitory factor (MIF) plays a protective role in infection control in TB; however, to date, no studies on antibody responses to MIF have been reported. We measured immunoglobulin (Ig)A and IgG responses to MIF in individuals with either active tuberculosis (ATB; n = 65), latent tuberculosis (LTBI; n = 53), or in non-infected individuals (NI; n = 62). The QuantiFERON-TB Gold In-Tube (QFT-GIT) assay was used in order to screen for LTBI. The level of IgA against MIF was significantly lower in LTBI and ATB patients than in NI individuals, was significantly related to LTBI and ATB diagnosis, and it could discriminate between LTBI and ATB. In contrast, the level of IgG against MIF was significantly lower in LTBI patients than in NI individuals and was significantly related to LTBI diagnosis. Anti-MIF IgG levels were significantly lower in AFB-negative TB, minimal TB, and new ATB patients, than in the NI group. IgA and IgG levels against MIF both showed significant negative correlations with IFN-γ levels, as assessed using the QFT-GIT test. Although none of the antibodies could achieve high diagnostic predictive power individually, our results suggest the possibility of using IgA antibody responses to MIF in the diagnosis of LTBI and ATB.

The protective role of macrophage migration inhibitory factor in acute kidney injury after cardiac surgery

2018 ◽  
Vol 10 (441) ◽  
pp. eaan4886 ◽  
Author(s):  
Christian Stoppe ◽  
Luisa Averdunk ◽  
Andreas Goetzenich ◽  
Josefin Soppert ◽  
Arnaud Marlier ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Cardiac Surgery ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Kidney Injury ◽  
Protective Role ◽  
Inhibitory Factor ◽  
Macrophage Migration

scholarly journals Macrophage Migration Inhibitory Factor Contributes to Host Defense against Acute Trypanosoma cruzi Infection

2006 ◽  
Vol 74 (6) ◽  
pp. 3170-3179 ◽  
Author(s):  
José L. Reyes ◽  
Luis I. Terrazas ◽  
Bertha Espinoza ◽  
David Cruz-Robles ◽  
Virgilia Soto ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Defense ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Inhibitory Factor ◽  
Interleukin 12 ◽  
Macrophage Migration ◽  
Necrosis Factor Alpha ◽  
Ifn Γ ◽  
Oxide Synthase

ABSTRACT Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that is involved in the host defense against several pathogens. Here we used MIF−/− mice to determine the role of endogenous MIF in the regulation of the host immune response against Trypanosoma cruzi infection. MIF−/− mice displayed high levels of blood and tissue parasitemia, developed severe heart and skeletal muscle immunopathology, and succumbed to T. cruzi infection faster than MIF+/+ mice. The enhanced susceptibility of MIF−/− mice to T. cruzi was associated with reduced levels of proinflammatory cytokines, such as tumor necrosis factor alpha, interleukin-12 (IL-12), IL-18, gamma interferon (IFN-γ), and IL-1β, in their sera and reduced production of IL-12, IFN-γ, and IL-4 by spleen cells during the early phase of infection. At all time points, antigen-stimulated splenocytes from MIF+/+ and MIF−/− mice produced comparable levels of IL-10. MIF−/− mice also produced significantly less Th1-associated antigen-specific immunoglobulin G2a (IgG2a) throughout the infection, but both groups produced comparable levels of Th2-associated IgG1. Lastly, inflamed hearts from T. cruzi-infected MIF−/− mice expressed increased transcripts for IFN-γ, but fewer for IL-12 p35, IL-12 p40, IL-23, and inducible nitric oxide synthase, compared to MIF+/+ mice. Taken together, our findings show that MIF plays a role in controlling acute T. cruzi infection.

scholarly journals Macrophage Migration Inhibitory Factor: A Downregulator of Early T Cell-Dependent IFN-γ Responses inPlasmodium chabaudi adami(556 KA)-Infected Mice

2011 ◽  
Vol 186 (11) ◽  
pp. 6271-6279 ◽  
Author(s):  
Diane Tshikudi Malu ◽  
Benoit Bélanger ◽  
François Desautels ◽  
Karine Kelendji ◽  
Esther Dalko ◽  
...  
Keyword(s):  
T Cell ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Inhibitory Factor ◽  
Macrophage Migration ◽  
Ifn Γ

Chimeric DNA vaccines encoding Eimeria acervulina macrophage migration inhibitory factor (E.MIF) induce partial protection against experimental Eimeria infection

2015 ◽  
Vol 60 (3) ◽  
Author(s):  
Xiaokai Song ◽  
Ruirui Zhang ◽  
Lixin Xu ◽  
Ruofeng Yan ◽  
Xiangrui Li
Keyword(s):  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Target Genes ◽  
Dna Vaccines ◽  
Inhibitory Factor ◽  
Macrophage Migration ◽  
Eimeria Acervulina ◽  
Duodenal Lesions ◽  
Ifn Γ

AbstractChimeric DNA vaccines co-expressing Eimeria acervulina macrophage migration inhibitory factor (E.MIF) and chicken IL-2 (IL-2) or interferon-γ (IFN-γ) were constructed and their efficacies against E. acervulina were evaluated. The open reading frame (ORF) of E.MIF was cloned from E. acervulina merozoites and subcloned into the eukaryotic expression vector pVAX1 with chicken cytokine gene IFN-γ or IL-2 to construct the DNA vaccines pVAX-E.MIF-IFN-γ, pVAX-E.MIF-IL-2 and pVAX-E.MIF. The in vivo transfection of the target genes was detected by use of reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Immunizations were carried out by vaccinating chickens twice with a dose rate of 100 μg intramuscularly. Seven days post second immunization, all chickens except the unchallenged control group were challenged orally with 1 × 105 sporulated oocysts of E. acervulina. Seven days later, the duodenum was collected. The results showed that the target genes were expressed effectively in vivo. DNA vaccines and the recombinant E.MIF protein could alleviate body weight loss and duodenal lesions significantly compared to the control groups. Furthermore, pVAX-E.MIF-IL-2 and pVAX-E.MIF-IFN-γ induced anticoccidial indexs (ACIs) of 179.12 and 170, respectively, which were significantly higher than that of pVAX-E.MIF (ACI = 162.31). Our results demonstrated that E.MIF is a potential vaccine candidate against E. acervulina and chicken IFN-γ or IL- 2 may be used as genetic adjuvants to improve the efficacies of DNA vaccines against avian coccidiosis.

scholarly journals Protective role of macrophage migration inhibitory factor in nonalcoholic steatohepatitis

2014 ◽  
Vol 28 (12) ◽  
pp. 5136-5147 ◽  
Author(s):  
Daniel Heinrichs ◽  
Marie‐Luise Berres ◽  
Melanie Coeuru ◽  
Meike Knauel ◽  
Andreas Nellen ◽  
...  
Keyword(s):  
Migration Inhibitory Factor ◽  
Nonalcoholic Steatohepatitis ◽  
Macrophage Migration Inhibitory Factor ◽  
Protective Role ◽  
Inhibitory Factor ◽  
Macrophage Migration
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