scholarly journals Impact of Chromosome 9 Numerical Imbalances in Oral Squamous Cell Carcinoma: A Pilot Grid-Based Centromere Analysis

Diagnostics ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 501
Author(s):  
Efthymios Kyrodimos ◽  
Aristeidis Chrysovergis ◽  
Nicholas Mastronikolis ◽  
Evangelos Tsiambas ◽  
Christos Riziotis ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Genetic Alterations ◽  
Chromosome 9 ◽  
Biological Behavior ◽  
Specific Gene ◽  
Tissue Sections ◽  
Grid Based

Oral squamous cell carcinoma (OSCC) is considered an aggressive malignancy, mainly due to its increased propensity to provide local and distant lymph node metastases. Gross chromosome instability (CI; polysomy/aneuploidy/monosomy), combined or not with specific gene alterations, is implicated in the development and progression of solid malignancies, including OSCC. In order to further study the relationship between these genetic alterations and the aggressive biological behavior of OSCCs, we investigated the frequency and impact of chromosome 9 numerical imbalances in these tumors. Fifty (n = 50) formalin-fixed, paraffin-embedded primary OSCC tissue sections were used. Chromogenic in situ hybridization (CISH) was implemented for detecting chromosome 9 (CEN—centromere enumeration) numerical alterations. Concerning the screening process in CISH slides, a novel, real-time reference and calibration grid platform was implemented. Chromosome 9 polysomy was observed in 8/50 (16%) tissue sections, whereas the rest of them demonstrated a normal, diploid pattern (42/50; 84%). Chromosome 9 polysomy was associated with the grade of differentiation of the examined tumors (p = 0.036). Chromosome 9 numerical imbalances (polysomy) were observed in sub-groups of OSCCs correlating with a progressive dedifferentiation of the malignant tissues. Concerning the implementation of the proposed grid-based platform as described above on CISH slides, it provides a novel, fast, and accurate screening mapping mechanism for detecting chromosome numerical imbalances.

Relationship Between mir15b and Sal-Like Protein 4 and Biological Behavior of Oral Squamous Cell Carcinoma

2021 ◽  
Vol 11 (2) ◽  
pp. 308-314
Author(s):  
Zengbo Wu ◽  
Yan Yan ◽  
Xianzhuo Chen ◽  
Yanling Liu ◽  
Dinggen Chen
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Normal Mucosa ◽  
Clinicopathological Features ◽  
Biological Behavior ◽  
Control Group ◽  
Tumor Tissues ◽  
Proliferation And Invasion

miR15b and SALL4 are involved in a variety of tumor progression. The roles of miR15b and SALL4 in oral squamous cell carcinoma (OSCC) remains unclear. The tumors and normal mucosa of OSCC patients were collected to detect miR15b and SALL4 level by Real-time PCR and analyze their correlation with OSCC clinicopathological features. Oral cancer Tca8113 cells were separated into control group; miR15b mimics group and miR15b inhibitor group followed by analysis of SALL4 expression, cell survival by MTT assay; cell invasion by Transwell chamber assay, as well as expression of N-cadherin and Vimentin and correlated with TNM stage, tumor volume and metastasis, and positively with differentiation TGF-β by Western blot. miR15b expression was decreased and SALL4 expression was increased in OSCC tumor tissues. miR15b was negatively degree (P < 0.05), whereas, opposite correlation of SALL4 with the above parameters was found (P < 0.05). miR15b and SALL4 were negatively correlated. MiR15b mimics significantly up-regulated MiR15b, decreased SALL4 expression, inhibited Tca8113 cell proliferation and invasion, as well as reduced N-cadherin, Vimentin and TGF-βexpression (P < 0.05). Opposite results were found in MiR15b inhibitor group. MiR15b expression is decreased and SALL 4 is increased in OSCC tumor tissues. MiR15b and SALL4 is closely related to OSCC clinicopathological features. MiR15b regulates the expression of EMT-related genes and TGF-β, thereby altering the proliferation and invasion of OSCC cells.

scholarly journals miR-222 regulates the cell biological behavior of oral squamous cell carcinoma by targeting PUMA

2014 ◽  
Vol 31 (3) ◽  
pp. 1255-1262 ◽  
Author(s):  
FANGFANG JIANG ◽  
WEI ZHAO ◽  
LIJIE ZHOU ◽  
LIN ZHANG ◽  
ZIFENG LIU ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Biological Behavior

scholarly journals Immunoexpression of P16 and Ki 67 in oral squamous cell carcinoma: Association with clinicopathological parameters.

2020 ◽  
Vol 3 (2) ◽  
pp. 135-144
Author(s):  
Shadan Omer ◽  
Payman Rashid
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Biological Behavior ◽  
Clinicopathological Parameters ◽  
Statistical Association ◽  
Ki 67 ◽  
Lower Lip ◽  
Significant Statistical Association

Background and Objectives: Oral squamous cell carcinoma (OSCC) is considered as a major health problem worldwide and has been associated with high recurrence rate and poor progno-sis. Advances in understanding of OSCC have not improved the outcome in their management significantly. Many studies have focused on the roles of biomolecular markers in OSCC. The use of p16 and Ki67 as biomarkers of biological behavior of oral squamous cell carcinoma is contro-versial. This study aimed to determine immunoexpression of P16 and Ki67 in oral squamous cell carcinoma and to evaluate their association with various clinicopathological parameters. Materials and Methods: Fifty cases of squamous cell carcinoma from different locations in the oral cavity were included in this cross sectional study. The cases were collected from Rizgary Teaching Hospital and Private Laboratories in Erbil city during a period of eight months from October 2018 to May 2019. The expression of p16 and Ki 67 were evaluated immunohistochem-ically; the findings were correlated with the age of the patients, gender, site of the tumor and grade of the tumor. Result: A total of 50 patients with oral squamous cell carcinoma were enrolled in this study the age ranged from 33 to 89 years, with a mean age ± SD of (64.24 ±12.01) years and more than half (52.0%) of them were males. Lower lip was the most common site of the tumor followed by upper lip and tongue (42.0%, 26.0% and 18.0%, respectively). Histopathological findings of the tumor showed that (54.0%) of the patients had moderately differentiated squamous cell carci-noma. However, (84.0%) of the patients showed negative expression of P 16, while Ki 67 ex-pression was positive among (76.0%) of them. No significant statistical association were found between immunoexpression of p16 and age, sex of patient, site of the tumor and grade of the tumor (P=0.67, P=0,095, P=0.696, P=0.454 respectively). No significant statistical association were found between immunoexpression of Ki67 and age, sex of patient, site of the tumor and grade of the tumor (P=0.637, P=0,411, P=0.353, P=1.00 respectively). Conclusion: in relation to the results obtained in this study no significant association were found between P16 and Ki 67 immunoexpression in oral squamous cell carcinoma with clinicopatho-logical parameters. Further researches have to be designed to better understand the role of p16 and Ki 67 in OSCC. Keywords: oral squamous cell carcinoma, immunoexpression, P16, Ki67.

scholarly journals Tumor-associated tissue eosinophilia in oral squamous cell carcinoma – A predictable biological behavior

2020 ◽  
Vol 3 ◽  
pp. 3-8
Author(s):  
Parikshya Shrestha ◽  
Keerthi Narayan ◽  
Varsha Vimal Kumar ◽  
Girish C. Hemadala ◽  
Sanjay Murgod
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Eosinophil Count ◽  
Malignant Neoplasm ◽  
Biological Behavior ◽  
Eosinophil Infiltration ◽  
Tissue Eosinophilia ◽  
Special Stain

Objectives: Squamous cell carcinoma of the oral cavity is the most common aggressive epithelial malignant neoplasm. Its biological behavior is influenced by the host immune cells, such as multifaceted eosinophils, associated with wound healing and tissue damage processes. Their presence within a variety of human cancers raises queries about their role. The infiltrations of tumor stroma by eosinophils are believed to play a significant role in progression of the carcinoma and could be either a potential diagnostic tool for stromal invasion or as a prognostic indicator. Its role in cancer still remains unclear since in the literature, there are very few studies showing improved prognosis and few contradictory studies showing poor prognosis. This study was conducted with an aim to compare the tumor-associated tissue eosinophilia in oral squamous cell carcinoma (OSCC) and normal tissue and to correlate the expression in different grades of carcinoma using a special stain that targets eosinophils exclusively and vividly. Materials and Methods: The study includes 30 samples, six normal, and 24 histopathologically diagnosed with OSCC. 5 μ thick sections were made and stained using special stain and examined under high power (×40), ten consecutive microscopic fields were studied. The average numbers of eosinophils were statistically analyzed. Results: Eosinophil count for carcinoma was higher compared to normal mucosa, but the comparison in different grades of cancer did not show much difference. Conclusion: Since eosinophil count was higher in carcinoma, eosinophil infiltration in dysplastic cases should prompt thorough evaluation for invasiveness.

scholarly journals Melatonin Inhibits the Progression of Oral Squamous Cell Carcinoma via Inducing miR-25-5p Expression by Directly Targeting NEDD9

2020 ◽  
Vol 10 ◽  
Author(s):  
Yanling Wang ◽  
Bo Tao ◽  
Jiaying Li ◽  
Xiaoqun Mao ◽  
Wei He ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Wound Healing ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Viability ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Colony Formation ◽  
Biological Behavior ◽  
Luciferase Reporter

Melatonin exerts anti-cancer roles in various types of cancers. However, to the best of our knowledge, its role in oral squamous cell carcinoma (OSCC) is unknown. The present study aimed to investigate the role of melatonin and its underlying mechanism in OSCC. MTT, colony formation, wound healing, and transwell invasion assays proved that melatonin played anti-tumor effects in OSCC cells by inhibiting cell viability, proliferation, migration, and invasion in a concentration-dependent manner. The RT-qPCR analysis showed that miR-25-5p was significantly upregulated after melatonin treatment. Further, miR-25-5p might be involved in melatonin-induced inhibitory effects on the biological behavior of OSCC. The expression of miR-25-5p was decreased in tumor tissues and OSCC cells detected by RT-qPCR. MTT assay, colony formation assay, and TUNEL staining indicated miR-25-5p overexpression inhibited OSCC cell viability, proliferation, and induced OSCC cell apoptosis. Furthermore, wound healing, transwell invasion assay, and animal experiments suggested that miR-25-5p might exert suppressive effects on the migration, invasion, and tumor formation of OSCC cells, while miR-25-5p knockdown exhibited the opposite effects in OSCC cells. Bioinformatics analysis, western blot analysis, and luciferase reporter assay suggested that neural precursor cell expressed developmentally downregulated protein 9 (NEDD9) was proved to be a putative target for miR-25-5p. The role of NEDD9 in inhibiting OSCC cell proliferation, invasion, and migration was verified with NEDD9 siRNA transfection. Thus, melatonin exerted anti-proliferative, anti-invasive, and anti-migrative effects on OSCC via miR-25-5p/NEDD9 pathway. Melatonin could be applied as a potential novel drug on treating OSCC.

scholarly journals Role of HPV-16 in Pathogenesis of Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma and Correlation of p16INK4A Expression in HPV-16 Positive Cases: An Immunohistochemical Study

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Gaurav Pralhad Agrawal ◽  
Priya Shirish Joshi ◽  
Anshita Agrawal
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Hpv Infection ◽  
Hpv 16 ◽  
Oral Epithelial Dysplasia ◽  
Tissue Sections ◽  
Oral Epithelial

The objective of current study is to evaluate the role of HPV-16 in the pathogenesis of oral epithelial dysplasias (OED) and oral squamous cell carcinoma (OSCC) by immunohistochemistry (IHC) and to know whether HPV-16 participates in disruption of the regulation of p16 INK4A suppressor protein in OED and OSCC by IHC. Histopathologically diagnosed 20 cases of OED and 20 cases of OSCC were selected from amongst the patients attending the OPD of Vasantdada Patil Dental College and Hospital, Sangli. Biopsy tissue section were then tested for HPV-16 by IHC. HPV-16 positive tissue sections were then again tested by p16 by IHC. Overall 22.5% of cases in our study were found to be positive for HPV 16 which includes 10% of cases of OED and 35% cases of OSCC. Amongst the HPV 16 positive cases, more than 60% of cells were positive for p16INK4A IHC in OED (50%) and OSCC (85.71%). Thus, HPV 16 participates in disruption of the regulation of p16INK4A suppressor protein and can be used as surrogate biomarker for detection of HPV infection in OED and OSCC.

scholarly journals Biological behavior of oral squamous cell carcinoma in the background of novel corona virus infection

Oral Oncology ◽  
2020 ◽  
Vol 110 ◽  
pp. 104781 ◽  
Author(s):  
Sachin C. Sarode ◽  
Gargi S. Sarode ◽  
Namrata Sengupta ◽  
Nilesh Kumar Sharma ◽  
Shankargouda Patil
Keyword(s):  
Squamous Cell Carcinoma ◽  
Virus Infection ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Biological Behavior ◽  
Corona Virus
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