scholarly journals Unstimulated Parotid Saliva Sampling in Juvenile Idiopathic Arthritis and Healthy Controls: A Proof-of-Concept Study on Biomarkers

Diagnostics ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 251
Author(s):  
Alexandra Dimitrijevic Carlsson ◽  
Bijar Ghafouri ◽  
Carin Starkhammar Johansson ◽  
Per Alstergren
Keyword(s):  
Juvenile Idiopathic Arthritis ◽  
Flow Rate ◽  
Total Protein ◽  
Salivary Flow ◽  
Inflammatory Biomarkers ◽  
Healthy Children ◽  
Proof Of Concept ◽  
Parotid Saliva ◽  
Meso Scale Discovery ◽  
Concept Study

The aims of this proof-of-concept study were to develop a collecting method for unstimulated parotid saliva in juvenile idiopathic arthritis (JIA) patients and healthy children and to investigate if inflammatory biomarkers could be detected in these samples. Forty-five children with JIA (median age of 12 years and 25th–75th percentile of 10–15 years; 33 girls and 12 boys) and 16 healthy children as controls (median age of 13 years and 25–75th percentile of 10–13 years; 11 girls and 5 boys) were enrolled in this study. Unstimulated parotid saliva was collected with a modified Carlson–Crittenden collector. The salivary flow rate and salivary concentrations of total protein and inflammatory mediators were assessed. The Meso Scale Discovery electrochemiluminescence immunoassay was used for analyzing protein concentrations and the inflammatory biomarkers. Sufficient parotid saliva volumes to be analyzed could be collected with the collection device. JIA patients had a lower sampling saliva volume (p = 0.008) and saliva flow rate (p = 0.039) than controls. The total protein concentrations and inflammatory biomarkers were measured in the last six healthy subjects. The median protein concentration was 1312 µg/mL (25th percentile: 844 µg/mL and 75th percentile: 2062 µg/mL; n = 6) and quantifiable concentrations of 39 inflammatory proteins could be assessed in these samples. In conclusion, this study indicates that the saliva sampling method, as used in the present study, is able to collect sufficient sample volumes in children, and that it is possible to analyze various inflammatory biomarkers in the collected saliva.

Dental Caries and Salivary Alterations in Type I Diabetes

2011 ◽  
Vol 36 (2) ◽  
pp. 181-184 ◽  
Author(s):  
Kavita Rai ◽  
Amitha Hegde ◽  
Ananth Kamath ◽  
Suchetha Shetty
Keyword(s):  
Dental Caries ◽  
Flow Rate ◽  
Salivary Flow ◽  
Salivary Flow Rate ◽  
Control Group ◽  
Diabetic Patients ◽  
Type I ◽  
Healthy Children ◽  
Salivary Ph ◽  
Anti Oxidant

Insulin dependent diabetes mellitus is a severe disease that raises blood glucose levels because of hyperglycemia and insulinopenia. Fluctuations in water and electrolyte levels may result in xerostomia and other changes in the salivary composition. Since diabetes has an influence on oral health, it is important for the dentist to be aware of newer advances in the field of diabetes and to recognize specific oral problems related to diabetes. Thus, the dentist becomes an important part of the health care team for the patients with diabetes. Aim: The present study correlated salivary flow rate, salivary pH and total salivary antioxidant levels and dental caries in type I diabetic patients. Method: A total of 200 children that included 100 known diabetic children (study group) and 100 healthy children (controls) of both the sexes and from similar socioeconomic backgrounds formed the part of this study. Dental caries was assessed using DMFT index. The salivary total anti-oxidant level was estimated using phospho molybdic acid using spectrophotometric method. The salivary flow rate was recorded using the Zunt method and the salivary pH using the pH indicating paper. The results were statistically analyzed using t-test. Conclusions: The analyzed parameters showed increase in salivary anti-oxidant levels, reduced salivary flow rate, increase incidence of dental caries, salivary pH was decreased when compared to the control group.

Description of Active Joint Count Trajectories in Juvenile Idiopathic Arthritis

2014 ◽  
Vol 41 (12) ◽  
pp. 2466-2473 ◽  
Author(s):  
Roberta A. Berard ◽  
George Tomlinson ◽  
Xiuying Li ◽  
Kiem Oen ◽  
Alan M. Rosenberg ◽  
...  
Keyword(s):  
Juvenile Idiopathic Arthritis ◽  
Patient Characteristics ◽  
Joint Disease ◽  
Latent Classes ◽  
Growth Curve Analysis ◽  
Proof Of Concept ◽  
Active Joint ◽  
Concept Study ◽  
Latent Growth Curve Analysis ◽  
Longitudinal Joint

Objective.To describe the trajectories of longitudinal joint disease activity in juvenile idiopathic arthritis (JIA), and to examine associations of clinical and laboratory characteristics with the identified trajectories.Methods.A retrospective cohort study at 2 Canadian centers was performed. The longitudinal trajectories of active joint counts were described in a proof-of-concept study using a latent growth curve analysis. Baseline patient characteristics were compared across trajectory groups.Results.Data were analyzed on 659 children diagnosed with JIA between March 1980 and September 2009. The median age at diagnosis was 10.0 years (interquartile range 3.7–13.4) and 61% (402/659) were female. The International League of Associations for Rheumatology (ILAR) diagnoses were as follows: oligoarthritis (36%), enthesitis-related arthritis (20%), rheumatoid factor (RF)-negative polyarthritis (13%), undifferentiated arthritis (12%), psoriatic arthritis (8%), systemic arthritis (7%), and RF-positive polyarthritis (4%). Based on the trajectories of their active joint counts, the 659 patients were each classified in 1 of 5 latent classes (which can be described as high decreasing, moderate increasing, persistent moderate, persistent low, and minimal joint activity). These latent classes were clinically and statistically distinct from the ILAR categories.Conclusion.In this proof-of-concept study, in which we used an analytic methodology in a novel way, we identified 5 clinically and statistically distinct trajectories of disease course. The subsets of patients within each class were different from those described by the ILAR classification criteria. This successful application of this method supports its use in a chronic disease with a fluctuating course such as JIA. These methods should be expanded for the purposes of predictive modeling.

scholarly journals Saliva diagnostic utility in patients with type 2 diabetes: Future standard method

2019 ◽  
Vol 0 (0) ◽  
Author(s):  
Marwa Mrag ◽  
Asma Kassab ◽  
Asma Omezzine ◽  
Raoua Belkacem Chebil ◽  
Fatma Ben Fredj Ismail ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Flow Rate ◽  
Total Protein ◽  
Immunoglobulin A ◽  
Salivary Flow ◽  
Salivary Flow Rate ◽  
Oral Exam ◽  
Whole Saliva ◽  
Significant Difference

Summary Background The purpose of the present study was to assess saliva reliability in diagnosis and monitoring type 2 diabetes instead of blood. Methods Blood and unstimulated whole saliva were collected from 300 type 2 diabetic subjects and 300 healthy controls in fasting. Then, the salivary flow rate was calculated. All parameters including glucose, urea, amylase, total protein, albumin, C-reactive protein (CRP), immunoglobulin A (IgA), potassium, calcium and chloride were assessed in the supernatant, using an autoanalyzer. Oral exam was conducted by a single examiner on full mouth excluding third molars. Statistical analysis was performed by the SPSS 20.0 version. Results Saliva screening showed that glucose, urea, amylase, total protein, potassium, calcium and chloride were significantly higher in patients compared to controls (p < 0.05). Whereas, the IgA level and salivary flow rate were significantly reduced in patients (p < 0.05). No significant difference was found in albumin and CRP levels (p = 0.05). There was a significant positive correlation between salivary and plasma glucose levels (r = 0.887, and r = 0.900, p < 0.001), as well as, salivary and blood urea (r = 0.586, and r = 0.688, p < 0.001) in patients and controls, respectively. Conclusions From this study, saliva could be suggested as a useful diagnostic tool for type 2 diabetes.

Association of salivary alpha-amylase and salivary flow rate with working memory functioning in healthy children

Stress ◽  
2019 ◽  
Vol 22 (6) ◽  
pp. 670-678
Author(s):  
Enrique Francisco Maldonado ◽  
Mari Nislin ◽  
Ana Martínez-Escribano ◽  
Laura Marín ◽  
Alfredo Enguix ◽  
...  
Keyword(s):  
Working Memory ◽  
Flow Rate ◽  
Salivary Flow ◽  
Salivary Flow Rate ◽  
Alpha Amylase ◽  
Healthy Children ◽  
Salivary Alpha Amylase ◽  
Memory Functioning

Salivary flow rate, total protein and pH in caries-free children and adolescents aged between 5 and 18 years

2019 ◽  
Vol 24 (2) ◽  
Author(s):  
Iwona Przywitowska ◽  
Urszula Kaczmarek ◽  
Grzegorz Bartnicki ◽  
Alina Wrzyszcz-Kowalczyk
Keyword(s):  
Children And Adolescents ◽  
Flow Rate ◽  
Total Protein ◽  
Age Groups ◽  
Salivary Flow ◽  
Protein Levels ◽  
Saliva Flow Rate ◽  
Ph Values ◽  
Mixed Saliva ◽  
Saliva Flow

Introduction. Data regarding salivary flow and the levels of salivary components in developmental age are scarce and not fully consistent. Aim. The aim of the study was to compare unstimulated mixed saliva flow rate, pH and total protein in children aged between 5 and 18 years to obtain information on the functional maturation of salivary glands during the developmental period. Material and methods. A total of 90 children and adolescents (both sexes) aged between 5 and 18 years were examined. All subjects were caries-free (ICDA II score zero). Unstimulated mixed saliva was sampled from all patients to assess pH, total protein and flow rate. The subjects were divided into age groups 5-6, 13-14 and 18 years. The study was approved by the Bioethics Committee of the University (No. Nr KB-335/2013). Results. Significantly lower salivary flow rates were observed in 5-6 year olds vs. 13-14 and 18-year-olds. In contrast, pH values were significantly higher in the youngest group compared to older age groups. Total protein was the lowest in 5-6 year olds, higher in 13-14 year olds and the highest at the age of 18 years (significant difference between age groups of 5-6 and 18 years). A decreasing trend in pH values and an increasing one in protein levels were observed between the age groups. Considering the entire group of subjects, a positive correlation between age and salivary flow rate and protein levels, and a negative correlation with pH were found. Moreover, pH and protein levels decreased with increasing salivary flow. Conclusions. Unstimulated mixed saliva flow rate and total protein increase, while pH levels decrease between the ages of 5 to 18 years.

Salivary Flow Rate, pH, Buffering Capacity, Total Protein, Oxidative Stress and Antioxidant Capacity in Children with and without Dental Caries

2018 ◽  
Vol 42 (6) ◽  
pp. 445-449 ◽  
Author(s):  
Sudharani A Pyati ◽  
R Naveen Kumar ◽  
Vinod Kumar ◽  
N H Praveen Kumar ◽  
K M Parveen Reddy
Keyword(s):  
Antioxidant Capacity ◽  
Flow Rate ◽  
Total Protein ◽  
Chemical Properties ◽  
Salivary Flow ◽  
Buffering Capacity ◽  
Salivary Flow Rate ◽  
Ph Buffering ◽  
Total Antioxidant ◽  
Ph Buffering Capacity

Objectives: To measure and compare the levels of salivary flow rate, pH, buffering capacity, total protein, malondialdehyde (MDA) and total antioxidant capacity (TAC) between caries active and caries free children and to study the correlation between the DMFS/dfs score and above salivary parameters in caries active children. Study design: 50 caries active (DMFS/dfs ≥ 5) and 50 caries free (DMFS/dfs = 0) children aged between 6 to 12 years were included in the study. From all the children, unstimulated, mid-morning saliva samples were collected and salivary flow rate was calculated. Salivary pH, buffering capacity, total protein, MDA and TAC were measured. Results: The mean levels of salivary flow rate, pH, buffering capacity were significantly decreased (p &lt; 0.05) and total protein, MDA and TAC were significantly increased (p &lt; 0.05) in caries active children when compared to caries free controls. There was a proportionate decrease (p &lt; 0.05) in salivary flow rate, pH and buffering capacity and proportionate increase (p &gt; 0.05) in salivary total protein, MDA and TAC as DMFS/dfs score increased in caries active children. Conclusions: Significant alteration in the levels of salivary flow rate, pH, total proteins, MDA and TAC and their correlation with DMFS/dfs score in caries active children suggest, the levels of these physico-chemical properties of saliva can act as strong indicators of caries status in children.

Salivary Flow Rate, Calcium, Urea, Total Protein, and Amylase Levels in Fanconi Anemia

2010 ◽  
Vol 32 (2) ◽  
pp. e46-e49 ◽  
Author(s):  
Tatiana Maria Folador Mattioli ◽  
Ana Cláudia Koubik ◽  
Marina de Oliveira Ribas ◽  
Beatriz Helena Sottile França ◽  
João Armando Brancher ◽  
...  
Keyword(s):  
Fanconi Anemia ◽  
Flow Rate ◽  
Total Protein ◽  
Salivary Flow ◽  
Salivary Flow Rate
Sign in / Sign up

Export Citation Format

Share Document