scholarly journals Comparative Study Regarding the Properties of Methylene Blue and Proflavine and Their Optimal Concentrations for In Vitro and In Vivo Applications

Diagnostics ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 223 ◽  
Author(s):  
Maria-Eliza Nedu ◽  
Mihaela Tertis ◽  
Cecilia Cristea ◽  
Alexandru Valentin Georgescu
Keyword(s):  
Methylene Blue ◽  
Fluorescent Dyes ◽  
Tissue Level ◽  
In Vivo Studies ◽  
Cell Nuclei ◽  
In Vivo Experiments ◽  
Bacteriostatic Agent ◽  
Anti Inflammatory

Methylene blue and proflavine are fluorescent dyes used to stain nucleic acid from the molecular level to the tissue level. Already clinically used for sentinel node mapping, detection of neuroendocrine tumors, methemoglobinemia, septic shock, ifosfamide-induced encephalopathy, and photodynamic inactivation of RNA viruses, the antimicrobial, anti-inflammatory, and antioxidant effect of methylene blue has been demonstrated in different in vitro and in vivo studies. Proflavine was used as a disinfectant and bacteriostatic agent against many gram-positive bacteria, as well as a urinary antiseptic involved in highlighting cell nuclei. At the tissue level, the anti-inflammatory effects of methylene blue protect against pulmonary, renal, cardiac, pancreatic, ischemic-reperfusion lesions, and fevers. First used for their antiseptic and antiviral activity, respectively, methylene blue and proflavine turned out to be excellent dyes for diagnostic and treatment purposes. In vitro and in vivo studies demonstrated that both dyes are efficient as perfusion and tissue tracers and permitted to evaluate the minimal efficient concentration in different species, as well as their pharmacokinetics and toxicity. This review aims to identify the optimal concentrations of methylene blue and proflavine that can be used for in vivo experiments to highlight the vascularization of the skin in the case of a perforasome (both as a tissue tracer and in vascular mapping), as well as their effects on tissues. This review is intended to be a comparative and critical presentation of the possible applications of methylene blue (MB) and proflavine (PRO) in the surgical field, and the relevant biomedical findings from specialized literature to date are discussed as well.

scholarly journals COVID-19 lessons for medical practice. Methylene blue is a time-tested innovation

KIDNEYS ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 36-41
Author(s):  
D.D. Ivanov
Keyword(s):  
Methylene Blue ◽  
Stone Formation ◽  
Carbon Monoxide Poisoning ◽  
Antimicrobial Effect ◽  
Renal Calculi ◽  
In Vivo Studies ◽  
Anti Inflammatory ◽  
Tract Infections

Significance of methylene blue (MB) in therapy of methemoglobinemia, malaria and urinary tract infections (UTI) is well known. The antimicrobial, anti-inflammatory, and antioxidant effect of MB has been demonstrated in different in vitro and in vivo studies. Necessity to find the remedy against COVID-19 attracted the interest of investigators and general practitioners to MB worldwide. The review includes various experiences of MB use, in particular in the patients treated with MB during the COVID-19 epidemic with a preventive effect, as well as own experience on this topic. MB is widely used as a dye in a variety of biological sciences applications — diagnostic procedures and the treatment of multiple disorders, including cyanide, and carbon monoxide poisoning, and is considered to be nontoxic. The beneficial effects of MB in the management of patients with multiple small renal calculi, especially with infected stones, and prevention of new stone formation were presented in a 5-year study. Moreover, due to MB antifungal potential and antimicrobial effect, it reduces symptoms of UTI, improves quality of life and could prevent the recurrence of disease. The patented complex of MB with sage leaf extract and American cranberry fruit extract (Pembina Blue®) demonstrated significant reduction of pain and spasm in cystitis patients. Given the limited number of available UTI therapies with concomitant increase in drug resistance, the demand for the search of new safe and effective UTI treatments are inevitable. Considering the fact that MB is FDA-approved compound that is already used for various therapeutic options, and also owing to the distinct antioxidant, antifungal, antibacterial and anti-inflammatory mechanisms presented in this review, MB could be considered as a promising drug for future.

ANTITUMOR EFFECT OF COLLOIDAL SILVER BISILICATE IN EXPERIMENTAL IN VITRO AND IN VIVO STUDIES

2019 ◽  
Vol 65 (5) ◽  
pp. 760-765
Author(s):  
Margarita Tyndyk ◽  
Irina Popovich ◽  
A. Malek ◽  
R. Samsonov ◽  
N. Germanov ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Tumor Growth ◽  
Tumor Cells ◽  
Human Tumor ◽  
Significant Inhibition ◽  
In Vivo Studies ◽  
Ehrlich Carcinoma ◽  
In Vivo Experiments

The paper presents the results of the research on the antitumor activity of a new drug - atomic clusters of silver (ACS), the colloidal solution of nanostructured silver bisilicate Ag6Si2O7 with particles size of 1-2 nm in deionized water. In vitro studies to evaluate the effect of various ACS concentrations in human tumor cells cultures (breast cancer, colon carcinoma and prostate cancer) were conducted. The highest antitumor activity of ACS was observed in dilutions from 2.7 mg/l to 5.1 mg/l, resulting in the death of tumor cells in all studied cell cultures. In vivo experiments on transplanted Ehrlich carcinoma model in mice consuming 0.75 mg/kg ACS with drinking water revealed significant inhibition of tumor growth since the 14th day of experiment (maximally by 52% on the 28th day, p < 0.05) in comparison with control. Subcutaneous injections of 2.5 mg/kg ACS inhibited Ehrlich's tumor growth on the 7th and 10th days of the experiment (p < 0.05) as compared to control.

scholarly journals The Challenging Melanoma Landscape: From Early Drug Discovery to Clinical Approval

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 3088
Author(s):  
Mariana Matias ◽  
Jacinta O. Pinho ◽  
Maria João Penetra ◽  
Gonçalo Campos ◽  
Catarina Pinto Reis ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
Drug Evaluation ◽  
Three Dimensional ◽  
Experimental Models ◽  
In Vivo Studies ◽  
Murine Models ◽  
In Vivo Experiments ◽  
Novel Therapeutic Strategies

Melanoma is recognized as the most dangerous type of skin cancer, with high mortality and resistance to currently used treatments. To overcome the limitations of the available therapeutic options, the discovery and development of new, more effective, and safer therapies is required. In this review, the different research steps involved in the process of antimelanoma drug evaluation and selection are explored, including information regarding in silico, in vitro, and in vivo experiments, as well as clinical trial phases. Details are given about the most used cell lines and assays to perform both two- and three-dimensional in vitro screening of drug candidates towards melanoma. For in vivo studies, murine models are, undoubtedly, the most widely used for assessing the therapeutic potential of new compounds and to study the underlying mechanisms of action. Here, the main melanoma murine models are described as well as other animal species. A section is dedicated to ongoing clinical studies, demonstrating the wide interest and successful efforts devoted to melanoma therapy, in particular at advanced stages of the disease, and a final section includes some considerations regarding approval for marketing by regulatory agencies. Overall, considerable commitment is being directed to the continuous development of optimized experimental models, important for the understanding of melanoma biology and for the evaluation and validation of novel therapeutic strategies.

scholarly journals Anti-Inflammatory Potential of Daturaolone from Datura innoxia Mill.: In Silico, In Vitro and In Vivo Studies

2021 ◽  
Vol 14 (12) ◽  
pp. 1248
Author(s):  
Muhammad Waleed Baig ◽  
Humaira Fatima ◽  
Nosheen Akhtar ◽  
Hidayat Hussain ◽  
Mohammad K. Okla ◽  
...  
Keyword(s):  
In Silico ◽  
Therapeutic Potential ◽  
In Vivo Studies ◽  
Metabolic Reaction ◽  
Datura Innoxia ◽  
In Vivo Models ◽  
Immobility Time ◽  
Anti Inflammatory

Exploration of leads with therapeutic potential in inflammatory disorders is worth pursuing. In line with this, the isolated natural compound daturaolone from Datura innoxia Mill. was evaluated for its anti-inflammatory potential using in silico, in vitro and in vivo models. Daturaolone follows Lipinski’s drug-likeliness rule with a score of 0.33. Absorption, distribution, metabolism, excretion and toxicity prediction show strong plasma protein binding; gastrointestinal absorption (Caco-2 cells permeability = 34.6 nm/s); no blood–brain barrier penetration; CYP1A2, CYP2C19 and CYP3A4 metabolism; a major metabolic reaction, being aliphatic hydroxylation; no hERG inhibition; and non-carcinogenicity. Predicted molecular targets were mainly inflammatory mediators. Molecular docking depicted H-bonding interaction with nuclear factor kappa beta subunit (NF-κB), cyclooxygenase-2, 5-lipoxygenase, phospholipase A2, serotonin transporter, dopamine receptor D1 and 5-hydroxy tryptamine. Its cytotoxicity (IC50) value in normal lymphocytes was >20 µg/mL as compared to cancer cells (Huh7.5; 17.32 ± 1.43 µg/mL). Daturaolone significantly inhibited NF-κB and nitric oxide production with IC50 values of 1.2 ± 0.8 and 4.51 ± 0.92 µg/mL, respectively. It significantly reduced inflammatory paw edema (81.73 ± 3.16%), heat-induced pain (89.47 ± 9.01% antinociception) and stress-induced depression (68 ± 9.22 s immobility time in tail suspension test). This work suggests a possible anti-inflammatory role of daturaolone; however, detailed mechanistic studies are still necessary to corroborate and extrapolate the findings.

scholarly journals Su1819 – New Class of Anti-Inflammatory Therapeutics Based on Gold (III) Complexes in Intestinal Inflammation – Proof of Concept Based on in Vitro and in Vivo Studies

2019 ◽  
Vol 156 (6) ◽  
pp. S-623
Author(s):  
Julia B. Krajewska ◽  
Jakub Wlodarczyk ◽  
Przemyslaw Taciak ◽  
Remigiusz Szczepaniak ◽  
Jakub Fichna
Keyword(s):  
Intestinal Inflammation ◽  
In Vivo Studies ◽  
Proof Of Concept ◽  
New Class ◽  
Anti Inflammatory

Anti-inflammatory effect ofRhus vernivifluaStokes by suppression of iNOS-mediated Akt and ERK pathways: in-vitro and in-vivo studies

2011 ◽  
Vol 63 (5) ◽  
pp. 679-687 ◽  
Author(s):  
Chang Hwa Jung ◽  
Jeong-Hyun Kim ◽  
Ji Hye Kim ◽  
Joo Hee Chung ◽  
Han-Seok Choi ◽  
...  
Keyword(s):  
In Vivo Studies ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals In Vitro and in Vivo Studies on Anti-Inflammatory Effects of Traditional Okinawan Vegetable Methanol Extracts

ACS Omega ◽  
2019 ◽  
Vol 4 (13) ◽  
pp. 15660-15664
Author(s):  
Junichi Nagata ◽  
Hiroyuki Yokodera ◽  
Goki Maeda
Keyword(s):  
In Vivo Studies ◽  
Methanol Extracts ◽  
Anti Inflammatory

scholarly journals Effect of Resveratrol on In Vitro and In Vivo Models of Diabetic Retinophathy: A Systematic Review

2019 ◽  
Vol 20 (14) ◽  
pp. 3503 ◽  
Author(s):  
Mario D. Toro ◽  
Katarzyna Nowomiejska ◽  
Teresio Avitabile ◽  
Robert Rejdak ◽  
Sarah Tripodi ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Risk Of Bias ◽  
In Vivo Studies ◽  
Exclusion Criteria ◽  
In Vivo Models ◽  
In Vivo Experiments ◽  
Retinal Pathology ◽  
Full Text Review

A large number of preclinical studies suggest the involvement of resveratrol in the prevention and treatment of eye diseases induced by oxidative stress and inflammation. We tested the hypothesis that resveratrol influences many pathways of in vitro and in vivo models of diabetic retinopathy through a systematic literature review of original articles. The review was conducted in accordance with the PRISMA guidelines. A literature search of all original articles published until April 2019 was performed. The terms “resveratrol” in combination with “retina”, “retinal pathology”, “diabetic retinopathy” and “eye” were searched. Possible biases were identified with the adopted SYRCLE’s tool. Eighteen articles met inclusion/exclusion criteria for full-text review. Eleven of them included in vitro experiments, 11 studies reported in vivo data and 3 studies described both in vitro and in vivo experiments. Most of the in vivo studies did not include data that would allow exclusion of bias risks, according to SYRCLE’s risk of bias tool. Both in vitro and in vivo data suggest anti-apoptotic, anti-inflammatory and anti-oxidative actions of resveratrol in models of diabetic retinopathy. However, results on its anti-angiogenic effects are contradictory and need more rigorous studies.

scholarly journals Ultrafine particles affect the balance of endogenous pro- and anti-inflammatory lipid mediators in the lung: in-vitro and in-vivo studies

2012 ◽  
Vol 9 (1) ◽  
pp. 27 ◽  
Author(s):  
Ingrid Beck-Speier ◽  
Erwin Karg ◽  
Heidrun Behrendt ◽  
Tobias Stoeger ◽  
Francesca Alessandrini
Keyword(s):  
Ultrafine Particles ◽  
Lipid Mediators ◽  
In Vivo Studies ◽  
Anti Inflammatory
Sign in / Sign up

Export Citation Format

Share Document