scholarly journals Does Endometriosis Influence the Embryo Quality and/or Development? Insights from a Large Retrospective Matched Cohort Study

Diagnostics ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 83 ◽  
Author(s):  
Ana M. Sanchez ◽  
Luca Pagliardini ◽  
Greta C. Cermisoni ◽  
Laura Privitera ◽  
Sofia Makieva ◽  
...  
Keyword(s):  
Potential Effect ◽  
Fertilization Rate ◽  
Women Patients ◽  
Cleavage Stage ◽  
Ongoing Pregnancy Rate ◽  
Vitro Fertilization ◽  
Morphological Evaluation ◽  
Negative Effect

In vitro fertilization can be an effective tool to manage the endometriosis-associated infertility, which accounts for 10% of the strategy indications. Nevertheless, a negative effect of endometriosis on IVF outcomes has been suggested. The aim of this study was to evaluate the potential effect of endometriosis in the development of embryos at cleavege stage in assisted reproduction treatment cycles. A total of 429 cycles from women previously operated for moderate/severe endometriosis were compared with 851 cycles from non-affected women. Patients were matched by age, number of oocyte retrieved and study period. A total of 3818 embryos in cleavage stage have been analyzed retrospectively. Overall, no difference was found between women with and without endometriosis regarding the number of cleavage stage embryos obtained as well as the percentage of good/fair quality embryos. Excluding cycles in which no transfers were performed or where embryos were frozen in day three, no difference was observed for blastulation rate or the percentage of good/fair blastocysts obtained. Despite similar fertilization rate and number/quality of embryos, a reduction in ongoing pregnancy rate was observed in patients affected, possibly due to an altered endometrial receptivity or to the limited value of the conventional morphological evaluation of the embryo.

Development and implementation of intracytoplasmic sperm injection (ICSI)

1995 ◽  
Vol 7 (2) ◽  
pp. 211 ◽  
Author(s):  
GD Palermo ◽  
J Cohen ◽  
M Alikani ◽  
A Adler ◽  
Z Rosenwaks
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Human Sperm ◽  
Fertilization Rate ◽  
Semen Parameters ◽  
Ongoing Pregnancy Rate ◽  
Vitro Fertilization ◽  
Human Oocytes ◽  
Intracytoplasmic Injection ◽  
Previously Treated

The purpose of this paper is to elucidate the experimental steps that led to the development of intracytoplasmic sperm injection (ICSI) and its application in the human. ICSI has become the most successful micromanipulation procedure for treating male infertility. A total of 355 in vitro fertilization (IVF) cycles utilizing ICSI are described; 180 couples were previously treated in 509 IVF cycles but achieved no fertilization and 175 couples could not be treated by IVF because of extremely poor semen parameters. Of the 3063 metaphase II (M II) oocytes retrieved, 2970 were injected with a survival rate of 93.6%, yielding 1917 bipronuclear zygotes (64.5%). In 148 patients, a foetal heart was evidenced by ultrasound; 11 of these patients miscarried between 7 and 13 weeks of gestation. The ongoing pregnancy rate was 38.6% (137/355) per retrieval and 40.5% (137/338) per embryo replacement. At the time of writing, there were 22 deliveries and one therapeutic abortion for a trisomy 21 chromosomal abnormality. In addition, 66 singleton, 37 twin, 10 triplet and 1 quadruplet pregnancies were ongoing. The concentration of motile spermatozoa in the ejaculate only slightly influenced the fertilization rate (P < 0.001) and the pregnancy outcome (P < 0.01). A preliminary injection procedure utilizing intracytoplasmic injection of isolated sperm heads was performed in 35 M II human oocytes with resultant fertilization and cleavage rates of 74% and 73% respectively. Skills in ICSI were acquired by injecting hamster and unfertilized human oocytes with human sperm. ICSI can be used to successfully treat couples who have failed IVF or who have too few spermatozoa for conventional in vitro insemination.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Biological and Biochemical factors Predictive of Oocyte survival, Fertilization, Pregnancy in oocyte thawing cycles

2021 ◽  
pp. 1-12
Author(s):  
Enrica Capitanio ◽  
Alessia Galimberti ◽  
Laura Zanga ◽  
Federica Paternostro ◽  
Sara Melis ◽  
...  
Keyword(s):  
Fertilization Rate ◽  
Clinical Pregnancy ◽  
Clinical Factors ◽  
Ongoing Pregnancy ◽  
Ongoing Pregnancy Rate ◽  
Vitro Fertilization ◽  
Factors Affecting ◽  
Thawing Cycle ◽  
Oocyte Freezing

Optimization and monitoring of IVF treatments requires good data on the effect and magnitude of clinical factors affecting treatment outcome. Many factors have been known to affect IVF outcomes. Currently there are still no data to predict whether a patient who undergoes In Vitro Fertilization (IVF) cycles can be considered a good candidate for oocyte freezing. The aim of this study was therefore to evaluate which biological and biochemical factors can be predictive of oocyte survival and fertilization, as well as of clinical pregnancy in oocyte thawing cycles. This study showed that none of the factors available on the day of the pick-up is able to predict (in case of oocyte cryopreservation) the success of a subsequent oocyte thawing cycle. Only the transfer of at least one Grade 1 embryo after oocyte thawing cycle has a statistically significant impact on pregnancy. Unfortunately, this cannot be considered an elective factor to guide the clinician and/or the embryologist in choosing patient's treatment as it is not available on the day of the oocyte pick up but it is a result of oocyte thawing. Keywords: Oocyte thawing; Biological and biochemical markers; Fertilization rate; Ongoing pregnancy rate

scholarly journals IN VITRO MATURATION OF BOVINE OOCYTES FOR IN VITRO FERTILIZATION

2009 ◽  
Vol 15 (1) ◽  
pp. 16-19
Author(s):  
Ioan GROZA ◽  
Simona CIUPE ◽  
Mihai CENARIU ◽  
Emoke PALL ◽  
Anamaria PETREAN
Keyword(s):  
In Vitro Fertilization ◽  
Embryonic Development ◽  
In Vitro Maturation ◽  
Cultivation Conditions ◽  
Vitro Fertilization ◽  
Morphological Evaluation ◽  
17Β Estradiol ◽  
Bovine Oocytes

The objective of the present study was to asses the quality of various cultivation media used for the maturation of bovine oocytes that are prepared for IVF. Upon collection from slaughtered bovine ovaries and after morphological evaluation, a total number of 513 viable oocytes have been selected for cultivation, being divided into 3 batches, 171 oocytes / batch. The oocytes belonging to batch 1 were cultivated in TCM 199 NaHCO3 + 10% FCS + FSH 20 μl/ml. The oocytes belonging to batch 2 were cultivated in TCM 199 NaHCO3 + 10% FCS + HCG 2.3 x 103 UI/ml + FSH 8 μl/ml + pyruvate 0.25 mM + 17β estradiol 1 μl/ml. The oocytes belonging to batch 3 were cultivated in TCM 199 NaHCO3 + 10% FCS + 17β estradiol 1 μl/ml + FSH 20 μl/ml. The cultivation conditions, for all three batches, were: 24 hours at 39°C, 5% CO2. Spermatozoa have been prepared using the Percoll method and IVF of the matured oocytes has been performed. Embryonic development has been assessed 72 hours and then up to 10 days after IVF. The results showed the superior quality of the oocytes belonging to batch 2 and matured using TCM 199 NaHCO3 + 10% FCS + HCG 2.3x103 UI/ml + FSH 8 μl/ml + pyruvate 0.25 mM + 17β estradiol 1 μl/ml, as their use for IVF yielded the highest number of viable embryos.

scholarly journals Pyometra does not affect some molecular quality-related parameters of canine oocytes

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Piotr Pawlak ◽  
Ewelina Warzych ◽  
Zofia E. Madeja ◽  
Tomasz Nowak ◽  
Dorota Lechniak
Keyword(s):  
Mrna Level ◽  
Oocyte Quality ◽  
Potential Effect ◽  
Animal Cloning ◽  
Maternal Effect Genes ◽  
Negative Effect ◽  
Reproductive Techniques ◽  
Two Parameters

AbstractCystic endometrial hyperplasia-pyometra complex (CEH/P) significantly perturbs the reproductive performance of affected bitches and ovariohysterectomy (OHE) is a commonly applied treatment. Thus the only way to take advantage of the genetic potential of valuable females is application of assisted reproductive techniques (ART) mainly in vitro embryo production (IVP) or in some exceptional cases animal cloning by somatic cell nuclear transfer (SCNT). The aim of our study was to examine a potential effect of the CEH/P status on the quality of oocytes from females subjected to OHE. In total, 828 immature oocytes collected from ovaries of 33 bitches (21 control, 12 CEH/P) were subjected to genetic analyses (mRNA expression of two maternal-effect genes: GDF-9, OCT4 and mitochondrial DNA – mtDNA - content). Oocytes of CEH/P females were characterized by a higher mtDNA content (471 696) than gametes of their healthy counterparts (368 175; p<0,005). Transcripts for the two genes were detected in all samples and the mRNA level was not affected by the CEH/P status. In conclusion, the CEH/P complex does not exert a negative effect on oocyte quality reflected by the two parameters examined in this study.

scholarly journals THE QUALITY OF EMBRYOS RESULTED FROM IN VITRO FERTILIZATION BY USING FROZEN SEMEN THAWED IN DIFFERENT TEMPERATURES

2017 ◽  
Vol 3 (1) ◽  
pp. 23
Author(s):  
Rakhmi Yasri ◽  
Ristika Handarini ◽  
Muhammad Imron
Keyword(s):  
In Vitro Fertilization ◽  
Key Words ◽  
Embryo Quality ◽  
Fertilization Rate ◽  
Test Results ◽  
Vitro Fertilization ◽  
Frozen Semen ◽  
Different Temperatures

In vitro fertilization technology in cows is an effort done to utilize ovary waste from cows slughtered in abbatoir. This study was aimed at assessing the qualiy of embryos resulted from in vitro fertilization by using frozen semen thawed in different temperatures. In order to get qualty semen, standardized thawing method is required. It was expected from this study that an optimum thawing temperature for frozen semen was determined to obtain quality transferable embryos. Three treatments consisting of thawing with water 37°C for 30 second (T1), thawing with water 25°C for 30 second (T2), and thawing with water 10°C for 30 second (T3). Data were subjected to an an anlysis of variance (Anova) and a Duncan test. Results showed that oocytes fertilized with frozen semen thawed at 37°C and 10°C had higher fertilization rate and excellent-grade embryos (P<0.05) than did the ones fertilized with frozen semen thawed at 25°C. However, no different effect of thawing temperatures was found on transferable and degenerated embryos (P>0.05). It was concluded that embryos fertilized with Brahman frozen semen in thawed at 37°C had the highest number of embryos (49.66±2.88) and excellent-grade embryos (22.00±4.35). Key words: Embryo quality, In vitro fertilization, frozen semen thawing, Brahman bull.

scholarly journals A Brief Incubation of Cumulus-Enclosed Mouse Eggs in a Calcium-Free Medium Containing a High Concentration of Calcium-Chelator Markedly Improves Preimplantation Development

2020 ◽  
Vol 17 (10) ◽  
pp. 3505
Author(s):  
Valeria Merico ◽  
Silvia Garagna ◽  
Maurizio Zuccotti
Keyword(s):  
In Vitro Fertilization ◽  
Mammalian Species ◽  
Developmental Rate ◽  
Cumulus Cells ◽  
Fertilization Rate ◽  
Preimplantation Development ◽  
High Concentration ◽  
Vitro Fertilization ◽  
Positive Effects

The presence of cumulus cells (CCs) surrounding ovulated eggs is beneficial to in vitro fertilization and preimplantation development outcomes in several mammalian species. In the mouse, this contribution has a negligible effect on the fertilization rate; however, it is not yet clear whether it has positive effects on preimplantation development. Here, we compared the rates of in vitro fertilization and preimplantation development of ovulated B6C3F1 CC-enclosed vs. CC-free eggs, the latter obtained either after a 5 min treatment in M2 medium containing hyaluronidase or after 5–25 min in M2 medium supplemented with 34.2 mM EDTA (M2-EDTA). We found that, although the maintenance of CCs around ovulated eggs does not increment their developmental rate to blastocyst, the quality of the latter is significantly enhanced. Most importantly, for the first time, we describe a further quantitative and qualitative improvement, on preimplantation development, when CC-enclosed eggs are isolated from the oviducts in M2-EDTA and left in this medium for a total of 5 min prior to sperm insemination. Altogether, our results establish an important advancement in mouse IVF procedures that would be now interesting to test on other mammalian species.

Sexual Function and Fertility Quality of Life in Women Using In Vitro Fertilization

2015 ◽  
Vol 12 (4) ◽  
pp. 985-993 ◽  
Author(s):  
Nicole K. Smith ◽  
Jody Madeira ◽  
Heather R. Millard
Keyword(s):  
Quality Of Life ◽  
In Vitro Fertilization ◽  
Sexual Function ◽  
Vitro Fertilization
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