scholarly journals Determination of Specific IgG to Identify Possible Food Intolerance in Athletes Using ELISA

Data ◽  
2021 ◽  
Vol 6 (11) ◽  
pp. 122
Author(s):  
Kristina Malsagova ◽  
Alexander Stepanov ◽  
Alexandra A. Sinitsyna ◽  
Alexander Izotov ◽  
Mikhail S. Klyuchnikov ◽  
...  
Keyword(s):  
Recovery Period ◽  
Food Intolerance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Dietary Recommendations ◽  
Peptide Antigens ◽  
Food Antigens ◽  
Adaptive Processes ◽  
Personalized Approach

Nutrition is considered one of the foundations of athletic performance, and post-workout nutritional recommendations are fundamental to the effectiveness of the recovery and adaptive processes. Therefore, at present, new directions in dietetics are being formed, focused on the creation of personalized diets. To identify the probable risk of somatic and allergic reactions upon contact with food antigens, we used the method of enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of IgG antibodies in the blood plasma of athletes against protein–peptide antigens accommodated in food. The study enrolled 40 athletes of boating and fighting sport disciplines. We found that the majority of the studied participants were characterized by an elevated IgG level against one or two food allergens (barley, almond, strawberry, etc.). Comparative analysis of the semiquantitative levels of IgG antibodies in athletes engaged in boating and fighting did not reveal significant differences between these groups. As a result, foods that are likely to cause the most pronounced immune response amongst the studied participants can be identified, which may indicate the presence of food intolerances. An athlete’s diet is influenced by both external and internal factors that can reduce or worsen the symptoms of a food intolerance/allergy associated with exercise. The range of foods is wide, and the effectiveness of a diet depends on the time, the place, and environmental factors. Therefore, during the recovery period (the post-competition period), athletes are advised to follow the instructions of doctors and nutritionists. An effective, comprehensive recovery strategy during the recovery period may enhance the adaptive response to fatigue, improving muscle function and increasing exercise tolerance. The data obtained may be useful for guiding the development of a new personalized approach and dietary recommendations covering the composition of athletes’ diet and the prevalence of food intolerance.

scholarly journals Technical and Diagnostic Performance of Five Commercial Anti-Diphtheria Toxoid IgG Enzyme-Linked Immunosorbent Assay Kits

2010 ◽  
Vol 17 (10) ◽  
pp. 1612-1616 ◽  
Author(s):  
A. Faruq ◽  
L. Dadson ◽  
H. Cox ◽  
F. Alcock ◽  
A. R. Parker
Keyword(s):  
Diagnostic Performance ◽  
Relative Sensitivity ◽  
Diphtheria Toxoid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Relative Specificity ◽  
Sensitivity Specificity ◽  
Antibody Levels ◽  
Good Agreement

ABSTRACT The technical and diagnostic performances of five commercially available enzyme-linked immunosorbent assays for the measurement of anti-diphtheria toxoid IgG antibodies were evaluated. There was good agreement between the relative sensitivities of the five assays, but the relative specificity of one of the assays differed from that of the other four assays. Three of the five assays possessed recoveries of the international reference material NIBSC 00/496 within the range of 90% to 110% at antibody levels >0.1 IU/ml. The data suggest that there are manufacture-dependent differences in relative sensitivity, specificity, and accuracy for the determination of anti-diphtheria toxoid IgG antibodies that could result in different diagnostic interpretations.

Validation of a chemiluminescent assay for specific SARS-CoV-2 antibody

2020 ◽  
Vol 58 (8) ◽  
pp. 1357-1364 ◽  
Author(s):  
Marie Tré-Hardy ◽  
Alain Wilmet ◽  
Ingrid Beukinga ◽  
Jean-Michel Dogné ◽  
Jonathan Douxfils ◽  
...  
Keyword(s):  
Clinical Performance ◽  
Elisa Test ◽  
Diagnostic Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Elisa Method ◽  
Serological Tests ◽  
Economy Of Scale

AbstractObjectivesFaced with the COVID-19 pandemic and its impact on the availability and quality of both therapeutic and diagnostic methods, the Belgian authorities have decided to launch a procedure for additional evaluation of the performance of serological tests offered for sale on the national territory. This has been proposed with a double aim: (1) an in-depth verification of the analytical and clinical performances presented by the manufacturer and (2) an economy of scale in terms of centralized validation for all the laboratories using the tests subject to evaluation.MethodsA retrospective validation study was conducted including the serum of 125 patients in order to determine the analytical and clinical performances of the LIAISON®SARS-CoV-2 from DiaSorin® detecting anti-SARS-CoV-2 IgG and to compare its clinical performance with the enzyme-linked immunosorbent assay (ELISA) test from Euroimmun®, one of the first commercially available tests allowing the detection of anti-SARS-CoV-2 IgA and IgG.ResultsThe performances of the LIAISON®SARS-CoV-2 satisfied all the acceptance criteria and provided “real world” analytical and clinical performances very close to the ones reported by the manufacturer in its insert kit. Comparison between the LIAISON®SARS-CoV-2 and the ELISA method did not reveal any difference between the two techniques in terms of sensitivities and specificities regarding the determination of the IgG.ConclusionsThis study reports the validation of the LIAISON®SARS-CoV-2 allowing to detect IgG antibodies specifically directed against SARS-CoV-2. The analytical and clinical performances are excellent, and the automation of the test offers important rates, ideal for absorbing an extension of testing.

scholarly journals IMMUNOLOGICAL EFFICIENCY OF THE TETANUS TOXOID USE FOR THE URGENT SPECIFIC TETANUS PREVENTION IN PATIENTS WITH THE WOUND INFECTION

2020 ◽  
pp. 54-57
Author(s):  
E. I. Hirka ◽  
M. S. Popov
Keyword(s):  
Wound Infection ◽  
Immunosorbent Assay ◽  
Tetanus Toxoid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Clostridium Tetani ◽  
Test Systems ◽  
Tetanus Immunity

Summary. Aim. The present study aimed to analyze the effectiveness of tetanus toxoid administration during the urgent specific tetanus prevention. Materials and methods. The determination of tetanus immunity levels in patients with the wound infection before immunization, then 2 and 4 weeks after vaccination. Studies of blood sera of patients were carried out in dynamics by enzyme-linked immunosorbent assay using a set of test systems for determining IgG antibodies to tetanus toxoid «Clostridium tetani toxin IgG», ELISA, Nova Tec Immundiagnostica GmbH, Germany. Results of the study were measured in International Units per milliliter (IU / ml). Results. The tetanus toxoid AP-Biolik using for the urgent specific tetanus prevention is induce protective tetanus immunity levels 2 weeks after vaccination and long-term specific immunity protection 4 weeks after vaccination. Conclusions. The immunological efficiency of AP-Biolik using was established during its applying for urgent specific tetanus prevention in patients with the wound infection.

scholarly journals AB0663 COMPARING METHODS FOR DETERMINING ANTIBODIES TO SARS-CoV-2 USING A RAPID TEST AND ENZYME-LINKED IMMUNOSORBENT ASSAY

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 1363.3-1364
Author(s):  
G. Gridneva ◽  
E. Aronova ◽  
S. Glukhova ◽  
M. Cherkasova ◽  
B. Belov ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
False Negative ◽  
Connective Tissue Diseases ◽  
Rapid Test ◽  
Test Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Igm Antibodies ◽  
History Of

Background:An accessible and sensitive and sensitive method for determining antibodies to a new coronavirus infection is often the key to timely provision of the necessary medical care to patients with rheumatic diseasesObjectives:Compare methods for determining antibodies to SARS-CoV-2 using a rapid test and enzyme-linked immunosorbent assay (ELISA)Methods:Methods for determining antibodies to SARS-CoV-2 using an express test (Chromatographic express test SARS-CoV-2 IgG / IgM (Xiamen Biotime Biotechnology, China)) and by ELISA (Reagent kit for enzyme immunoassay of class G immunoglobulins and class M to SARS-CoV-2 (Vector-Best, Russia)) were compared.80 patients were included with a diagnosis of rheumatoid arthritis 26 (33%), psoriatic arthritis - 9 (11%), osteoarthritis - 15 (19%), rheumatic heart disease 1 (1%), SLE 2 (3%), deramtomyositis 3 (4%), systemic sclerosis 5 (6%), systemic connective tissue diseases 4 (5%), including Sjogren’s syndrome, spondyloarthritis 15 (19%).17 (21%) denied a history of COVID-19 symptoms. 63 (79%) noted any signs of COVID-19 3.095 ± 1.45 months before the test (Median 3 [2; 4] months). 63 (79%) noted any signs of COVID-19 109 ± 43 days before the test (Median 111 [78; 135] months). The ELISA method was considered the standard.Results:When comparing the results of the express test and the determination of IgG antibodies to SARS-CoV-2 in serum, the following was obtained: the sensitivity of the express test is 99%. When comparing the results of the express test and the determination of IgM antibodies to SARS-CoV-2 in serum, it was obtained: among 66 samples with a negative result by the express test method, IgM was detected in 6 cases by ELISA/ So, 7.5% of 80 samples were false negative. In 3 of 14 samples with a positive result by the express test, IgM by ELISA was not detected. So, 3.75% of 80 samples were false-positive. (Table 1). When comparing the results of the IgM express test and ELISA, the following was obtained: the sensitivity of the express test was 33%, the specificity was 85%.Table 1.Antibodies, express-testAntibodies (ELISA), absentAntibodies (ELISA), presentRowTotalsIgGabsent011Row %0.00%100.00%present37679Row %3.80%96.20%Totals37780IgМabsent60666Row %90.91%9.09%present11314Row %78.57%21.43%Totals71980Conclusion:When comparing the results of the express test and the determination of IgG antibodies to SARS-CoV-2 in serum, the sensitivity of the express test is 99%. Determination of IgM antibodies to SARS-CoV-2 using a rapid test is less reliable than determination using ELISA.Disclosure of Interests:None declared.

An Enzyme Linked Immunosorbent Assay for Determination of Tissue Plasminogen Activator Applied to Patients with Thromboembolic Disease

1983 ◽  
Vol 50 (03) ◽  
pp. 740-744 ◽  
Author(s):  
Nils Bergsdorf ◽  
Torbjörn Nilsson ◽  
Per Wallén
Keyword(s):  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Specific Activity ◽  
Thromboembolic Disease ◽  
Tissue Type ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type Plasminogen Activator ◽  
Tissue Plasminogen ◽  
Normal Human

SummaryUtilizing the immunoglobulin fraction from a goat antiserum against human uterine tissue plasminogen activator, an enzyme- linked immunoassay for tissue-type plasminogen activator in human plasma has been developed. With the new method, the concentration of t-PA in normal human acidified plasma is found to be 4.0 ± 1.8 (SD) ng/ml. It increases to 12 ng/ml after a tomiquet test, and to 14 ng/ml after strenous physical exercise. In a group of patients with idiopathic thromboembolic disease, the resting t-PA concentration was 5 ng/ml and the post-occlusion value 16 ng/ml. Furthermore, the patients also exhibited a normal post-occlusion rise in the concentration of plasmin-α2-antiplasmin complex. However, in 37% of the post-occlusion patient plasmas, virtually no increase in t-PA could be detected by a specific activity assay. The results indicate that the reason for a defective post-occlusion fibrinolytic activity in a majority of cases may be the presence of increased concentrations of a fast-acting specific t-PA inhibitor.

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