scholarly journals Molecular Analysis of Bacterial Community DNA in Sludge Undergoing Autothermal Thermophilic Aerobic Digestion (ATAD): Pitfalls and Improved Methodology to Enhance Diversity Recovery

Diversity ◽  
2010 ◽  
Vol 2 (4) ◽  
pp. 505-526 ◽  
Author(s):  
Anna V. Piterina ◽  
John Bartlett ◽  
J. Tony Pembroke
Keyword(s):  
Bacterial Community ◽  
Molecular Analysis ◽  
Aerobic Digestion ◽  
Thermophilic Aerobic Digestion ◽  
Autothermal Thermophilic Aerobic Digestion

scholarly journals A Unique Autothermal Thermophilic Aerobic Digestion Process Showing a Dynamic Transition of Physicochemical and Bacterial Characteristics from the Mesophilic to the Thermophilic Phase

2018 ◽  
Vol 84 (6) ◽  
Author(s):  
Yukihiro Tashiro ◽  
Kosuke Kanda ◽  
Yuya Asakura ◽  
Toshihiko Kii ◽  
Huijun Cheng ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Power Consumption ◽  
Electric Power ◽  
Full Scale ◽  
Final Phase ◽  
Aerobic Digestion ◽  
Content Type ◽  
Human Excreta ◽  
Thermophilic Aerobic Digestion ◽  
Autothermal Thermophilic Aerobic Digestion

ABSTRACTA unique autothermal thermophilic aerobic digestion (ATAD) process has been used to convert human excreta to liquid fertilizer in Japan. This study investigated the changes in physicochemical and bacterial community characteristics during the full-scale ATAD process operated for approximately 3 weeks in 2 different years. After initiating simultaneous aeration and mixing using an air-inducing circulator (aerator), the temperature autothermally increased rapidly in the first 1 to 2 days with exhaustive oxygen consumption, leading to a drastic decrease and gradual increase in oxidation-reduction potential in the first 2 days, reached >50°C in the middle 4 to 6 days, and remained steady in the final phase. Volatile fatty acids were rapidly consumed and diminished in the first 2 days, whereas the ammonia nitrogen concentration was relatively stable during the process, despite a gradual pH increase to 9.3. Principal-coordinate analysis of 16S rRNA gene amplicons using next-generation sequencing divided the bacterial community structures into distinct clusters corresponding to three phases, and they were similar in the final phase in both years despite different transitions in the middle phase. The predominant phyla (closest species, dominancy) in the initial, middle, and final phases wereProteobacteria(Arcobacter trophiarum, 19 to 43%;Acinetobacter towneri, 6.3 to 30%),Bacteroidetes(Moheibacter sediminis, 43 to 54%), andFirmicutes(Thermaerobacter composti, 11 to 28%;Heliorestis baculata, 2.1 to 16%), respectively. Two predominant operational taxonomic units (OTUs) in the final phase showed very low similarities to the closest species, indicating that the process is unique compared with previously published ones. This unique process with three distinctive phases would be caused by the aerator with complete aeration.IMPORTANCEAlthough the autothermal thermophilic aerobic digestion (ATAD) process has several advantages, such as a high degradation capacity, a short treatment period, and inactivation of pathogens, one of the factors limiting its broad application is the high electric power consumption for aerators with a full-scale bioreactor. We elucidated the dynamics of the bacterial community structures, as well as the physicochemical characteristics, in the ATAD process with a full-scale bioreactor from human excreta for 3 weeks. Our results indicated that this unique process can be divided into three distinguishable phases by an aerator with complete aeration and showed a possibility of shortening the digestion period to approximately 10 days. This research not only helps to identify which bacteria play significant roles and how the process can be improved and controlled but also demonstrates an efficient ATAD process with less electric power consumption for worldwide application.

scholarly journals Impact on degradation of antibiotics from poultry litter using Autothermal Thermophilic Aerobic Digestion (ATAD)

2021 ◽  
Vol 28 (1) ◽  
pp. 988-992
Author(s):  
Arivalagan Pugazhendhi ◽  
Sridevi Dhanarani Theivaraj ◽  
Gowri Manogari Boovaragamoorthy ◽  
Veerasamy Veeramani ◽  
Kathirvel Brindhadevi ◽  
...  
Keyword(s):  
Poultry Litter ◽  
Aerobic Digestion ◽  
Thermophilic Aerobic Digestion ◽  
Autothermal Thermophilic Aerobic Digestion

Efficiency of Autothermal Thermophilic Aerobic Digestion Under Two Different Oxygen Flow Rates

2009 ◽  
Vol 2009 (3) ◽  
pp. 397-408
Author(s):  
Sebnem Koyunluoglu Aynur ◽  
Sonali Dohale ◽  
Muriel Dumit ◽  
Rumana Riffat ◽  
Mohammad Abu-Orf ◽  
...  
Keyword(s):  
Oxygen Flow ◽  
Aerobic Digestion ◽  
Flow Rates ◽  
Thermophilic Aerobic Digestion ◽  
Autothermal Thermophilic Aerobic Digestion
Sign in / Sign up

Export Citation Format

Share Document