scholarly journals Peculiarities of Protein Crystal Nucleation and Growth

Crystals ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 422 ◽  
Author(s):  
Christo Nanev
Keyword(s):  
Crystallization Process ◽  
Protein Crystallization ◽  
Nucleation And Growth ◽  
Crystal Nucleation ◽  
Protein Crystal ◽  
Solution Flow ◽  
Molecular Scale ◽  
Novel Approaches ◽  
Lattice Formation ◽  
Complete Account

This paper reviews investigations on protein crystallization. It aims to present a comprehensive rather than complete account of recent studies and efforts to elucidate the most intimate mechanisms of protein crystal nucleation. It is emphasized that both physical and biochemical factors are at play during this process. Recently-discovered molecular scale pathways for protein crystal nucleation are considered first. The bond selection during protein crystal lattice formation, which is a typical biochemically-conditioned peculiarity of the crystallization process, is revisited. Novel approaches allow us to quantitatively describe some protein crystallization cases. Additional light is shed on the protein crystal nucleation in pores and crevices by employing the so-called EBDE method (equilibration between crystal bond and destructive energies). Also, protein crystal nucleation in solution flow is considered.

Control of Protein Crystal Nucleation and Growth Using Stirring Solution

2004 ◽  
Vol 43 (No. 11A) ◽  
pp. L1442-L1444 ◽  
Author(s):  
Ai Niino ◽  
Hiroaki Adachi ◽  
Kazufumi Takano ◽  
Hiroyoshi Matsumura ◽  
Takayoshi Kinoshita ◽  
...  
Keyword(s):  
Nucleation And Growth ◽  
Crystal Nucleation ◽  
Protein Crystal

Enhancement of nucleation of protein crystals on nano-wrinkled surfaces

2016 ◽  
Vol 186 ◽  
pp. 187-197 ◽  
Author(s):  
Praveen K. Bommineni ◽  
Sudeep N. Punnathanam
Keyword(s):  
Free Energy ◽  
Protein Concentration ◽  
Protein Crystallization ◽  
Crystal Nucleation ◽  
Protein Crystal ◽  
Protein Crystals ◽  
Free Energy Barrier ◽  
High Quality ◽  
High Quality Protein ◽  
Sinusoidal Surface

The synthesis of high quality protein crystals is essential for determining their structure. Hence the development of strategies to facilitate the nucleation of protein crystals is of prime importance. Recently, Ghatak and Ghatak [Langmuir 2013, 29, 4373] reported heterogeneous nucleation of protein crystals on nano-wrinkled surfaces. Through a series of experiments on different proteins, they were able to obtain high quality protein crystals even at low protein concentrations and sometimes without the addition of a precipitant. In this study, the mechanism of protein crystal nucleation on nano-wrinkled surfaces is studied through Monte Carlo simulations. The wrinkled surface is modeled by a sinusoidal surface. Free-energy barriers for heterogeneous crystal nucleation on flat and wrinkled surfaces are computed and compared. The study reveals that the enhancement of nucleation is closely related to the two step nucleation process seen during protein crystallization. There is an enhancement of protein concentration near the trough of the sinusoidal surface which aids in nucleation. However, the high curvature at the trough acts as a deterrent to crystal nucleus formation. Hence, significant lowering of the free-energy barrier is seen only if the increase in the protein concentration at the trough is very high.

A double cell for controlling nucleation and growth of protein crystals

1989 ◽  
Vol 22 (2) ◽  
pp. 115-118 ◽  
Author(s):  
M. Przybylska
Keyword(s):  
Crystal Growth ◽  
Protein Crystallization ◽  
Heavy Atom ◽  
Nucleation And Growth ◽  
The Other ◽  
Crystal Nucleation ◽  
Diffusion Method ◽  
Vapour Diffusion ◽  
Reservoir Solution ◽  
The Stability

A simple device for protein crystallization is described that consists of two connected cells, one for the hanging- or sitting-drop vapour diffusion method and the other for changing the concentration of the reservoir solution. It has been found useful for decoupling crystal nucleation from crystal growth, for improving the size and the stability of crystals, and in the preparation of heavy-atom derivatives.

Beneficial Effect of Solubility Enhancers on Protein Crystal Nucleation and Growth

Langmuir ◽  
2009 ◽  
Vol 25 (8) ◽  
pp. 4579-4587 ◽  
Author(s):  
Rajendrakumar A. Gosavi ◽  
Venkateswarlu Bhamidi ◽  
Sasidhar Varanasi ◽  
Constance A. Schall
Keyword(s):  
Beneficial Effect ◽  
Nucleation And Growth ◽  
Crystal Nucleation ◽  
Protein Crystal ◽  
Solubility Enhancers

scholarly journals The bee Tetragonula builds its comb like a crystal

2020 ◽  
Vol 17 (168) ◽  
pp. 20200187 ◽  
Author(s):  
Silvana S. S. Cardoso ◽  
Julyan H. E. Cartwright ◽  
Antonio G. Checa ◽  
Bruno Escribano ◽  
Antonio J. Osuna-Mascaró ◽  
...  
Keyword(s):  
Crystal Growth ◽  
Lattice Model ◽  
Stingless Bees ◽  
Nucleation And Growth ◽  
Coupled Map Lattice ◽  
Three Dimensions ◽  
Crystal Nucleation ◽  
Target Pattern ◽  
Brood Comb ◽  
Molecular Scale

Stingless bees of the genus Tetragonula construct a brood comb with a spiral or a target pattern architecture in three dimensions. Crystals possess these same patterns on the molecular scale. Here, we show that the same excitable-medium dynamics governs both crystal nucleation and growth and comb construction in Tetragonula , so that a minimal coupled-map lattice model based on crystal growth explains how these bees produce the structures seen in their bee combs.

scholarly journals Raman Spectroscopy of Protein Crystal Nucleation and Growth

2014 ◽  
Vol 10 (3) ◽  
pp. 202-207 ◽  
Author(s):  
Pechkova Eugenia ◽  
Parshina Evgenia ◽  
Maksimov Evgenii ◽  
Kutusov Nikolai ◽  
Brazhe Nadezda ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Nucleation And Growth ◽  
Crystal Nucleation ◽  
Protein Crystal
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