scholarly journals Phenomenological Consideration of Protein Crystal Nucleation; the Physics and Biochemistry behind the Phenomenon

Crystals ◽  
2017 ◽  
Vol 7 (7) ◽  
pp. 193 ◽  
Author(s):  
Christo Nanev
2013 ◽  
Vol 20 (4) ◽  
pp. 1049-1056 ◽  
Author(s):  
Masatoshi Maeki ◽  
Yuki Teshima ◽  
Saori Yoshizuka ◽  
Hiroshi Yamaguchi ◽  
Kenichi Yamashita ◽  
...  

Nanoscale ◽  
2018 ◽  
Vol 10 (26) ◽  
pp. 12256-12267 ◽  
Author(s):  
Mike Sleutel ◽  
Alexander E. S. Van Driessche

A historical overview and state-of-the-art analysis of the mechanism of protein crystal nucleation from an experimentalist's perspective.


2003 ◽  
Vol 36 (5) ◽  
pp. 1295-1296 ◽  
Author(s):  
Peter Nollert

The use of lipidic cubic phases as crystal nucleation and growth matrices is becoming popular and has yielded crystals of soluble and membrane proteins. So far, all of the membrane proteins crystallized by this method have been colored. This feature has facilitated the detection of the often encountered microcrystals in initial screening rounds. Indeed, small colorless protein crystals have poor optical contrast as a result of the small differences in refractive index of the protein crystal and the surrounding lipidic cubic phase. While a perfect preparation of a lipidic cubic phase is transparent and optically isotropic, in a crystallization setup it frequently disguises crystals due to cracks, inclusions, surface distortions and phase boundaries. Here, several specialized microscopic techniques and illumination conditions are compared and it is found that sufficient contrast is generated by cross polarization microscopy and by Hoffman modulation contrast microscopy for the detection of colorless protein crystals.


2008 ◽  
Vol 112 (15) ◽  
pp. 4725-4730 ◽  
Author(s):  
Bin Chen ◽  
Ricky B. Nellas ◽  
Samuel J. Keasler

2005 ◽  
Vol 275 (1-2) ◽  
pp. e1527-e1532 ◽  
Author(s):  
Anita Penkova ◽  
Olga Gliko ◽  
Ivaylo L. Dimitrov ◽  
Feyzim V. Hodjaoglu ◽  
Christo Nanev ◽  
...  

2004 ◽  
Vol 43 (No. 11A) ◽  
pp. L1442-L1444 ◽  
Author(s):  
Ai Niino ◽  
Hiroaki Adachi ◽  
Kazufumi Takano ◽  
Hiroyoshi Matsumura ◽  
Takayoshi Kinoshita ◽  
...  

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