scholarly journals Monitoring the Production of High Diffraction-Quality Crystals of Two Enzymes in Real Time Using In Situ Dynamic Light Scattering

Crystals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 65
Author(s):  
Raphaël de Wijn ◽  
Kévin Rollet ◽  
Sylvain Engilberge ◽  
Alastair G. McEwen ◽  
Oliver Hennig ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Growth Conditions ◽  
Psychrophilic Bacterium ◽  
X Ray Diffraction ◽  
Crystallization System ◽  
Rational Production ◽  
Hen Egg White ◽  
High Diffraction

The reproducible preparation of well-diffracting crystals is a prerequisite for every structural study based on crystallography. An instrument called XtalController has recently been designed that allows the monitoring of crystallization assays using dynamic light scattering and microscopy, and integrates piezo pumps to alter the composition of the mother liquor during the experiment. We have applied this technology to study the crystallization of two enzymes, the CCA-adding enzyme of the psychrophilic bacterium Planococcus halocryophilus, and the lysozyme from hen egg white in the presence of a synthetic chemical nucleant. We were able to (i) detect early nucleation events and (ii) drive the crystallization system (through cycles of dissolution/crystallization) toward growth conditions yielding crystals with excellent diffraction properties. This technology opens a way to the rational production of samples for crystallography, ranging from nanocrystals for electron diffraction, microcrystals for serial or conventional X-ray diffraction, to larger crystals for neutron diffraction.

scholarly journals Monitoring the production of high diffraction-quality crystals of two enzymes in real time using in situ dynamic light scattering

2020 ◽  
Author(s):  
Raphaël de Wijn ◽  
Kévin Rollet ◽  
Sylvain Engilberge ◽  
Alastair G. McEwen ◽  
Oliver Hennig ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Growth Conditions ◽  
Psychrophilic Bacterium ◽  
X Ray Diffraction ◽  
Egg White Lysozyme ◽  
Crystallization System ◽  
Rational Production ◽  
Hen Egg White

ABSTRACTThe reproducible preparation of well diffracting crystals is a prerequisite for every structural study based on crystallography. An instrument called the XtalController has recently been designed that allows the monitoring of crystallization assays using dynamic light scattering and microscopy, and integrates piezo pumps to alter the composition of the mother liquor during the experiment. We have applied this technology to study the crystallization of two enzymes, the CCA-adding enzyme of the psychrophilic bacterium Planococcus halocryophilus and the hen egg white lysozyme in the presence of a synthetic chemical nucleant. We were able to i) detect early nucleation events and ii) drive the crystallization system (through cycles of dissolution/crystallization) towards growth conditions yielding crystals with excellent diffraction properties. This technology opens a way to the rational production of samples for crystallography, ranging from nanocrystals for electron diffraction, microcrystals for serial or conventional X-ray diffraction, to larger crystals for neutron diffraction.

Aqueous solubilization of hydrophobic supramolecular metal–organic nanocapsules

2014 ◽  
Vol 5 (6) ◽  
pp. 2554-2559 ◽  
Author(s):  
H. Kumari ◽  
S. R. Kline ◽  
J. L. Atwood
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Neutron Scattering ◽  
Metal Organic

Micelles of surfactant solubilized metal-seamed pyrogallol[4]arene based organic nanocapsules are synthesized and characterized using in situ neutron scattering and dynamic light scattering techniques, which show trends in sizes as a function of alkyl tails of pyrogallols and surfactants.

Vesicles made of PS-PI cyclic diblock copolymers: In situ freeze-drying cryo-TEM and dynamic light scattering experiments

2005 ◽  
Vol 128 ◽  
pp. 163 ◽  
Author(s):  
Jean-Luc Putaux ◽  
Edson Minatti ◽  
Christelle Lefebvre ◽  
Redouane Borsali ◽  
Michel Schappacher ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Freeze Drying ◽  
Diblock Copolymers

In Situ Dynamic-Light-Scattering Study of a Cellular Interface Arising During Directional Growth

1991 ◽  
Vol 15 (2) ◽  
pp. 197-202
Author(s):  
J.-M Laherrère ◽  
J.-C Tolédano ◽  
H Savary ◽  
R Mellet
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Directional Growth ◽  
Scattering Study

Dynamic Light Scattering at CdSe Nanocrystals and CdSe Cluster-Molecules

2000 ◽  
Vol 636 ◽  
Author(s):  
A. Eichhöfer ◽  
C.V. Hänisch ◽  
M. Jacobsohn ◽  
U. Banin
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
High Performance ◽  
Size Range ◽  
Blue Shift ◽  
Cdse Nanocrystals ◽  
Particle Sizes ◽  
X Ray Diffraction ◽  
X Ray

AbstractHigh performance dynamic light scattering (DLS) has been used to determine the hydrodynamic diameters of CdSe-nanocrystals as well as CdSe cluster molecules in a size range of 1 to 10 nm. The method enables the determination of the particle sizes including the ligand shells directly in solution. Consistent with the blue shift of the absorption onset by reducing the particle sizes the values for the hydrodynamic radii decrease. For the CdSe nanoparticles the measured sizes were compared with the diameters determined by TEM experiments; the sizes of the cluster molecules were on the other hand estimated from the space filling models of the single crystal X-ray structure determination. Regarding both types of compounds, DLS yield comparable values for the radii, indicating that it provides a potentially important tool in addition to other size determining techniques like TEM, which operates at more drastic conditions, and powder X-ray diffraction being difficult to interpret below 5 nm.

In Situ Heteroepitaxial Bi2Sr2CaCu2O8 Thin Films Prepared by Metalorganic Chemical Vapor Deposition

1993 ◽  
Vol 335 ◽  
Author(s):  
Frank Dimeo ◽  
Bruce W. Wessels ◽  
Deborah A. Neumayer ◽  
Tobin J. Marks ◽  
Jon L. Schindler ◽  
...  
Keyword(s):  
Thin Films ◽  
Chemical Vapor Deposition ◽  
Vapor Deposition ◽  
Metalorganic Chemical Vapor Deposition ◽  
Chemical Vapor ◽  
Oxide Phase ◽  
Growth Conditions ◽  
X Ray Diffraction ◽  
Metalorganic Chemical

AbstractBi2Sr2CaCu2O8 thin films have been prepared in situ by low pressure metalorganic chemical vapor deposition using fluorinated β–diketonate precursors. The influence of the growth conditions on the oxide phase stability and impurity phase formation was examined as well as the superconducting properties of the films. Thin films deposited on LaAIO3 substrates were epitaxial as confirmed by x-ray diffraction measurements, including θ-2θ and φ scans. Four probe resistivity measurements showed the films to be superconducting with a maximum Tc0 of 90 K without post annealing. This Tc0 is among the highest reported for thin films of the BSCCO (2212) phase, and approaches reported bulk values.

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