scholarly journals Mammalian Cell Behavior on Hydrophobic Substrates: Influence of Surface Properties

2019 ◽  
Vol 3 (2) ◽  
pp. 48 ◽  
Author(s):  
Michele Ferrari ◽  
Francesca Cirisano ◽  
M. Carmen Morán
Keyword(s):  
Cell Adhesion ◽  
Surface Chemistry ◽  
Cell Viability ◽  
Surface Charge ◽  
Surface Properties ◽  
Mammalian Cell ◽  
Cell Biology ◽  
Biomedical Applications ◽  
Cell Behavior ◽  
Water Repellent

The influence of different surface properties holding to a modification of the substrate towards hydrophobic or superhydrophobic behavior was reviewed in this paper. Cell adhesion, their communication, and proliferation can be strongly manipulated, acting on interfacial relationship involving stiffness, surface charge, surface chemistry, roughness, or wettability. All these features can play mutual roles in determining the final properties of biomedical applications ranging from fabrics to cell biology devices. The focus of this work is the mammalian cell viability in contact with moderate to highly water repellent coatings or materials and also in combination with hydrophilic areas for more specific application. Few case studies illustrate a range of examples in which these surface properties and design can be fruitfully matched to the specific aim.

scholarly journals Functionalized Poly(ethylene glycol)-Based Bioassay Surface Chemistry That Facilitates Bio-Immobilization and Inhibits Nonspecific Protein, Bacterial, and Mammalian Cell Adhesion

2007 ◽  
Vol 19 (18) ◽  
pp. 4405-4414 ◽  
Author(s):  
Gregory M. Harbers ◽  
Kazunori Emoto ◽  
Charles Greef ◽  
Steven W. Metzger ◽  
Heather N. Woodward ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Ethylene Glycol ◽  
Surface Chemistry ◽  
Mammalian Cell ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Hydrodynamically-Confined Microflows for Cell Adhesion Strength Measurement

2010 ◽  
Author(s):  
Kevin V. Christ ◽  
Kevin T. Turner
Keyword(s):  
Cell Adhesion ◽  
Adhesion Strength ◽  
Cell Biology ◽  
Microfluidic Devices ◽  
Cell Behavior ◽  
Shear Stresses ◽  
Strength Measurement ◽  
Coated Surfaces ◽  
Biology Research ◽  
Standard Techniques

Cell adhesion plays a fundamental role in numerous physiological and pathological processes, and measurements of the adhesion strength are important in fields ranging from basic cell biology research to the development of implantable biomaterials. Our group and others have recently demonstrated that microfluidic devices offer advantages for characterizing the adhesion of cells to protein-coated surfaces [1,2]. Microfluidic devices offer many advantages over conventional assays, including the ability to apply high shear stresses in the laminar regime and the opportunity to directly observe cell behavior during testing. However, a key disadvantage is that such assays require cells to be cultured inside closed microchannels. Assays based on closed channels restrict the types of surfaces that can be examined and are not compatible with many standard techniques in cell biology research. Furthermore, while techniques for cell culture in microchannels have become common, maintaining the viability of certain types of cells in channels remains a challenge.

Fabrication of Ordered Sub-Micron Topographies on Large-Area Poly(Urethane Urea) by Two-Stage Replication Molding

2004 ◽  
Vol 820 ◽  
Author(s):  
Keith R. Milner ◽  
Mallory Balmer ◽  
Henry J. Donahue ◽  
Alan J. Snyder ◽  
Christopher A. Siedlecki
Keyword(s):  
Cell Adhesion ◽  
Surface Chemistry ◽  
Cell Behavior ◽  
Material Surface ◽  
Biological Cell ◽  
Large Area ◽  
Molding Process ◽  
Two Stage ◽  
Cell Material Interactions

AbstractIt has been established that material surface topography can have a significant effect on biological cell adhesion, in the absence of changes in surface chemistry. Such investigations were typically performed using surface features with size on the order of microns, comparable to the dimensions of the cells. It has been demonstrated that sub-micron sized topographies that cannot be created via contact lithography also influence cell behavior. The ability to affect cell adhesion is a prime consideration in the development of novel biomaterials. This study reports a two-stage replication molding process for fabricating ordered sub-micron sized features over a large area of biomedical polyether(urethane urea). Such a technique has great applicability in the area of long-term implantable materials as a method for influencing cell-material interactions.

scholarly journals NANOPATTERNED INTERFACES FOR CONTROLLING CELL BEHAVIOR

Nano LIFE ◽  
2010 ◽  
Vol 01 (01n02) ◽  
pp. 63-77 ◽  
Author(s):  
KEVIN CHUNG ◽  
JESSICA A. DeQUACH ◽  
KAREN L. CHRISTMAN
Keyword(s):  
Gene Expression ◽  
Cell Adhesion ◽  
Surface Chemistry ◽  
Semiconductor Industry ◽  
Cell Behavior ◽  
Adhesion Receptors ◽  
Cell Responses ◽  
Nanoscale Fabrication

Many studies have demonstrated that microscale changes to surface chemistry and topography affect cell adhesion, proliferation, differentiation, and gene expression. More recently, studies have begun to examine cell behavior interactions with structures on the nanoscale since in vivo, cells recognize and adhere to cell adhesion receptors that are spatially organized on this scale. These studies have been enabled through various fabrication methods, many of which were initially developed for the semiconductor industry. This review explores cell responses to a variety of controlled topographical and biochemical cues using an assortment of nanoscale fabrication methods in order to elucidate which pattern dimensions are beneficial for controlling cell adhesion and differentiation.

scholarly journals Special Issue: Biointerface Coatings for Biomaterials and Biomedical Applications

Coatings ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 423
Author(s):  
Hsien-Yeh Chen ◽  
Peng-Yuan Wang
Keyword(s):  
Surface Properties ◽  
Material Science ◽  
Biomedical Applications ◽  
Material Surface ◽  
Special Issue

The success of recent material science and applications in biotechnologies should be credited to developments of malleable surface properties, as well as the adaptation of conjugation reactions to the material surface [...]

scholarly journals Influence of Graphene Quantum Dots Surface Charge on their Uptake and Clearance in Melanoma Cells

2021 ◽  
Author(s):  
Lakshmi Narashimhan Ramana ◽  
Le N.M. Dinh ◽  
Vipul Agarwal
Keyword(s):  
Quantum Dots ◽  
Surface Charge ◽  
Biomedical Applications ◽  
Graphene Quantum Dots ◽  
Melanoma Cells ◽  
Rapid Clearance

Graphene quantum dots (GQDs) continue to draw interest in biomedical applications. However, their efficacy gets compromised due to their rapid clearance from body. On one side, rapid clearance is desired...

scholarly journals Protein and Polysaccharide-Based Electroactive and Conductive Materials for Biomedical Applications

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4499
Author(s):  
Xiao Hu ◽  
Samuel Ricci ◽  
Sebastian Naranjo ◽  
Zachary Hill ◽  
Peter Gawason
Keyword(s):  
Cell Biology ◽  
Biomedical Applications ◽  
Human Life ◽  
Electrically Conductive ◽  
Conductive Materials ◽  
Production Strategies ◽  
Integral Role ◽  
Material Sciences ◽  
Novel Drug

Electrically responsive biomaterials are an important and emerging technology in the fields of biomedical and material sciences. A great deal of research explores the integral role of electrical conduction in normal and diseased cell biology, and material scientists are focusing an even greater amount of attention on natural and hybrid materials as sources of biomaterials which can mimic the properties of cells. This review establishes a summary of those efforts for the latter group, detailing the current materials, theories, methods, and applications of electrically conductive biomaterials fabricated from protein polymers and polysaccharides. These materials can be used to improve human life through novel drug delivery, tissue regeneration, and biosensing technologies. The immediate goal of this review is to establish fabrication methods for protein and polysaccharide-based materials that are biocompatible and feature modular electrical properties. Ideally, these materials will be inexpensive to make with salable production strategies, in addition to being both renewable and biocompatible.

Stable Conjugates of Peptides with Gold Nanorods for Biomedical Applications with Reduced Effects on Cell Viability

2013 ◽  
Vol 5 (10) ◽  
pp. 4076-4085 ◽  
Author(s):  
Carolina Adura ◽  
Simon Guerrero ◽  
Edison Salas ◽  
Luis Medel ◽  
Ana Riveros ◽  
...  
Keyword(s):  
Cell Viability ◽  
Gold Nanorods ◽  
Biomedical Applications

scholarly journals Promising biocompatible hydrogels of crosslinked polyelectrolytes for biomedical applications

2017 ◽  
Vol 3 (2) ◽  
pp. 695-698
Author(s):  
Andreas Brietzke ◽  
Christian von der Ehe ◽  
Sabine Illner ◽  
Claudia Matschegewski ◽  
Niels Grabow ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Ionic Liquids ◽  
Cell Viability ◽  
Biomedical Applications ◽  
High Potential ◽  
Cell Viability Assay ◽  
Mouse Fibroblasts ◽  
Viability Assay ◽  
Intelligent Implant ◽  
L929 Mouse

AbstractFor the development of intelligent implant systems hydrogels (HG) from crosslinked ionic liquids feature a high potential to be utilised as a drug depot. Biocompatibility of the HGs is one key prerequisite for biomedical applications. HGs were polymerised from a variety of different ionic monomers based on methacrylate, methacrylamide, styrene or vinyl imidazolium derivatives in aqueous solution. N,N'-methylenebisacrylamide was used as crosslinker. CellQuanti-Blue™ Cell Viability Assay Kit was implemented to proof viability of L929 mouse fibroblasts. The predominant part of the HG eluates generated only a marginal reduction of less than 15% cell viability at 100% eluate concentration. This underlines the excellent suitability of these HGs for biomedical applications and revealed some promising candidates for the development of drug depots for implants.

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