scholarly journals Light Energy Partitioning under Various Environmental Stresses Combined with Elevated CO2 in Three Deciduous Broadleaf Tree Species in Japan

Climate ◽  
2019 ◽  
Vol 7 (6) ◽  
pp. 79 ◽  
Author(s):  
Mitsutoshi Kitao ◽  
Hiroyuki Tobita ◽  
Satoshi Kitaoka ◽  
Hisanori Harayama ◽  
Kenichi Yazaki ◽  
...  
Keyword(s):  
Elevated Co2 ◽  
Tree Species ◽  
Adaptive Response ◽  
Environmental Stresses ◽  
Light Energy ◽  
Photochemical Quenching ◽  
N Limitation ◽  
Elevated O3 ◽  
Broadleaf Tree ◽  
Non Photochemical Quenching

Understanding plant response to excessive light energy not consumed by photosynthesis under various environmental stresses, would be important for maintaining biosphere sustainability. Based on previous studies regarding nitrogen (N) limitation, drought in Japanese white birch (Betula platyphylla var. japonica), and elevated O3 in Japanese oak (Quercus mongolica var. crispula) and Konara oak (Q. serrata) under future-coming elevated CO2 concentrations, we newly analyze the fate of absorbed light energy by a leaf, partitioning into photochemical processes, including photosynthesis, photorespiration and regulated and non-regulated, non-photochemical quenchings. No significant increases in the rate of non-regulated non-photochemical quenching (JNO) were observed in plants grown under N limitation, drought and elevated O3 in ambient or elevated CO2. This suggests that the risk of photodamage caused by excessive light energy was not increased by environmental stresses reducing photosynthesis, irrespective of CO2 concentrations. The rate of regulated non-photochemical quenching (JNPQ), which contributes to regulating photoprotective thermal dissipation, could well compensate decreases in the photosynthetic electron transport rate through photosystem II (JPSII) under various environmental stresses, since JNPQ+JPSII was constant across the treatment combinations. It is noteworthy that even decreases in JNO were observed under N limitation and elevated O3, irrespective of CO2 conditions, which may denote a preconditioning-mode adaptive response for protection against further stress. Such an adaptive response may not fully compensate for the negative effects of lethal stress, but may be critical for coping with non-lethal stress and regulating homeostasis. Regarding the three deciduous broadleaf tree species, elevated CO2 appears not to influence the plant responses to environmental stresses from the viewpoint of susceptibility to photodamage.

scholarly journals DAY-LENGTH-DEPENDENT DELAYED-GREENING1, the Arabidopsis Homolog of the Cyanobacterial H+-Extrusion Protein, Is Essential for Chloroplast pH Regulation and Optimization of Non-Photochemical Quenching

2019 ◽  
Vol 60 (12) ◽  
pp. 2660-2671 ◽  
Author(s):  
Kyohei Harada ◽  
Takatoshi Arizono ◽  
Ryoichi Sato ◽  
Mai Duy Luu Trinh ◽  
Akira Hashimoto ◽  
...  
Keyword(s):  
Ph Regulation ◽  
Regulatory Protein ◽  
Protein A ◽  
Continuous Light ◽  
Membrane Vesicles ◽  
Light Energy ◽  
Day Length ◽  
Chloroplast Envelope ◽  
Photochemical Quenching ◽  
Non Photochemical Quenching

Abstract Plants convert solar energy into chemical energy through photosynthesis, which supports almost all life activities on earth. Because the intensity and quality of sunlight can change dramatically throughout the day, various regulatory mechanisms help plants adjust their photosynthetic output accordingly, including the regulation of light energy accumulation to prevent the generation of damaging reactive oxygen species. Non-photochemical quenching (NPQ) is a regulatory mechanism that dissipates excess light energy, but how it is regulated is not fully elucidated. In this study, we report a new NPQ-regulatory protein named Day-Length-dependent Delayed-Greening1 (DLDG1). The Arabidopsis DLDG1 associates with the chloroplast envelope membrane, and the dldg1 mutant had a large NPQ value compared with wild type. The mutant also had a pale-green phenotype in developing leaves but only under continuous light; this phenotype was not observed when dldg1 was cultured in the dark for ≥8 h/d. DLDG1 is a homolog of the plasma membrane-localizing cyanobacterial proton-extrusion-protein A that is required for light-induced H+ extrusion and also shows similarity in its amino-acid sequence to that of Ycf10 encoded in the plastid genome. Arabidopsis DLDG1 enhances the growth-retardation phenotype of the Escherichia coli K+/H+ antiporter mutant, and the everted membrane vesicles of the E. coli expressing DLDG1 show the K+/H+ antiport activity. Our findings suggest that DLDG1 functionally interacts with Ycf10 to control H+ homeostasis in chloroplasts, which is important for the light-acclimation response, by optimizing the extent of NPQ.

Shade lichens are characterized by rapid relaxation of non-photochemical quenching on transition to darkness

2021 ◽  
Vol 53 (5) ◽  
pp. 409-414
Author(s):  
Richard P. Beckett ◽  
Farida V. Minibayeva ◽  
Kwanele W. G. Mkhize
Keyword(s):  
Chlorophyll Fluorescence ◽  
Quantum Yield ◽  
Light Energy ◽  
Photochemical Quenching ◽  
Photosynthetic Organisms ◽  
Light Levels ◽  
Lower Light ◽  
Optimal Productivity ◽  
Sun And Shade ◽  
Non Photochemical Quenching

AbstractNon-photochemical quenching (NPQ) plays an important role in protecting photosynthetic organisms from photoinhibition by dissipating excess light energy as heat. However, excess NPQ can greatly reduce the quantum yield of photosynthesis at lower light levels. Recently, there has been considerable interest in understanding how plants balance NPQ to ensure optimal productivity in environments in which light levels are rapidly changing. In the present study, chlorophyll fluorescence was used to study the induction and relaxation of non-photochemical quenching (NPQ) in the dark and the induction of photosynthesis in ten species of lichens, five sampled from exposed and five sampled from shaded habitats. Here we show that the main difference between sun and shade lichens is the rate at which NPQ relaxes in the dark, rather than the speed that photosynthesis starts upon illumination. During the first two minutes in the dark, NPQ values in the five sun species declined only by an average of 2%, while by contrast, in shade species the average decline was 40%. For lichens growing in microhabitats where light levels are rapidly changing, rapid relaxation of NPQ may enable their photobionts to use the available light most efficiently.

scholarly journals Non-Photochemical Quenching. A Response to Excess Light Energy

2001 ◽  
Vol 125 (4) ◽  
pp. 1558-1566 ◽  
Author(s):  
Patricia Müller ◽  
Xiao-Ping Li ◽  
Krishna K. Niyogi
Keyword(s):  
Light Energy ◽  
Photochemical Quenching ◽  
Excess Light ◽  
Non Photochemical Quenching

scholarly journals Light compensation points in shade-grown seedlings of deciduous broadleaf tree species with different successional traits raised under elevated CO2

Plant Biology ◽  
2015 ◽  
Vol 18 ◽  
pp. 22-27 ◽  
Author(s):  
M. Kitao ◽  
T. Hida ◽  
N. Eguchi ◽  
H. Tobita ◽  
H. Utsugi ◽  
...  
Keyword(s):  
Elevated Co2 ◽  
Tree Species ◽  
Broadleaf Tree

scholarly journals The chloroplast NADPH thioredoxin reductase C, NTRC, controls non-photochemical quenching of light energy and photosynthetic electron transport inArabidopsis

2016 ◽  
Vol 39 (4) ◽  
pp. 804-822 ◽  
Author(s):  
Belén Naranjo ◽  
Clara Mignée ◽  
Anja Krieger-Liszkay ◽  
Dámaso Hornero-Méndez ◽  
Lourdes Gallardo-Guerrero ◽  
...  
Keyword(s):  
Electron Transport ◽  
Thioredoxin Reductase ◽  
Photosynthetic Electron Transport ◽  
Light Energy ◽  
Photochemical Quenching ◽  
Non Photochemical Quenching

scholarly journals Light potentials of photosynthetic energy storage in the field: what limits the ability to use or dissipate rapidly increased light energy?

2021 ◽  
Vol 8 (12) ◽  
Author(s):  
Atsuko Kanazawa ◽  
Abhijnan Chattopadhyay ◽  
Sebastian Kuhlgert ◽  
Hainite Tuitupou ◽  
Tapabrata Maiti ◽  
...  
Keyword(s):  
Environmental Conditions ◽  
Electron Flow ◽  
Crop Productivity ◽  
Open Science ◽  
High Light ◽  
Light Energy ◽  
Photochemical Quenching ◽  
Plant Photosynthesis ◽  
Photosynthetic Control ◽  
Non Photochemical Quenching

The responses of plant photosynthesis to rapid fluctuations in environmental conditions are critical for efficient conversion of light energy. These responses are not well-seen laboratory conditions and are difficult to probe in field environments. We demonstrate an open science approach to this problem that combines multifaceted measurements of photosynthesis and environmental conditions, and an unsupervised statistical clustering approach. In a selected set of data on mint ( Mentha sp.), we show that ‘light potentials’ for linear electron flow and non-photochemical quenching (NPQ) upon rapid light increases are strongly suppressed in leaves previously exposed to low ambient photosynthetically active radiation (PAR) or low leaf temperatures, factors that can act both independently and cooperatively. Further analyses allowed us to test specific mechanisms. With decreasing leaf temperature or PAR, limitations to photosynthesis during high light fluctuations shifted from rapidly induced NPQ to photosynthetic control of electron flow at the cytochrome b 6 f complex. At low temperatures, high light induced lumen acidification, but did not induce NPQ, leading to accumulation of reduced electron transfer intermediates, probably inducing photodamage, revealing a potential target for improving the efficiency and robustness of photosynthesis. We discuss the implications of the approach for open science efforts to understand and improve crop productivity.

Photosynthesis, light energy partitioning, and photoprotection in the shade-demanding species Panax notoginseng under high and low level of growth irradiance

2016 ◽  
Vol 43 (6) ◽  
pp. 479 ◽  
Author(s):  
Jun-Wen Chen ◽  
Shuang-Bian Kuang ◽  
Guang-Qiang Long ◽  
Sheng-Chao Yang ◽  
Zhen-Gui Meng ◽  
...  
Keyword(s):  
Panax Notoginseng ◽  
High Light ◽  
Light Energy ◽  
Quantum Yields ◽  
Photochemical Quenching ◽  
Light Illumination ◽  
Low Light ◽  
Psii Photochemistry ◽  
Growth Irradiance ◽  
Non Photochemical Quenching

Partitioning of light energy into several pathways and its relation to photosynthesis were examined in a shade-demanding species Panax notoginseng (Burkill) F.H.Chen ex C.Y.Wu & K.M.Feng grown along a light gradient. In fully light-induced leaves, the actual efficiency of PSII photochemistry (ΔF/Fmʹ), electron transport rate (ETR), non-photochemical quenching (NPQ) and photochemical quenching (qP) were lower in low-light-grown plants; this was also the case in fully dark-adapted leaves under a simulated sunfleck. In response to varied light intensity, high-light-grown plants showed greater quantum yields of light-dependent non-photochemical quenching (ΦNPQ) and PSII photochemistry (ΦPSII) and smaller quantum yields of fluorescence and constitutive thermal dissipation (Φf,d). Under the simulated sunfleck, high-light-grown plants showed greater ΦPSII and smaller Φf,d. There were positive relationships between net photosynthesis (Anet) and ΦNPQ+f,d and negative relationships between Anet and ΦPSII in fully light-induced leaves; negative correlations of Anet with ΦNPQ+f,d and positive correlations of Anet with ΦPSII were observed in fully dark-adapted leaves. In addition, more nitrogen was partitioned to light-harvesting components in low-light-grown plants, whereas leaf morphology and anatomy facilitate reducing light capture in high-light-grown plants. The pool of xanthophyll pigments and the de-epoxidation state was greater in high-light-grown plants. Antioxidant defence was elevated by increased growth irradiance. Overall, the evidences from P. notoginseng suggest that in high-light-grown shade-demanding plants irradiated by high light more electrons were consumed by non-net carboxylative processes that activate the component of NPQ, that low-light-grown plants correspondingly protect the photosynthetic apparatus against photodamage by reducing the efficiency of PSII photochemistry under high light illumination, and that during the photosynthetic induction, the ΔpH-dependent (qE) component of NPQ might dominate photoprotection, but the NPQ also depresses the enhancement of photosynthesis via competition for light energy.

Combining Gas Exchange and Chlorophyll Fluorescence to Assess the Adaptability of Medicinal Plant Aesculus chinensis Compared to Two Imported Aesculus Species

2013 ◽  
Vol 726-731 ◽  
pp. 4330-4336
Author(s):  
Hai Yan Fu ◽  
Fu Qiang Song ◽  
Jia Sen Wu ◽  
Xiang Shi Kong ◽  
Dan Dan Qi
Keyword(s):  
Chlorophyll Fluorescence ◽  
Electron Transport ◽  
Gas Exchange ◽  
Tree Species ◽  
Light Energy ◽  
The Other ◽  
Photochemical Quenching ◽  
Chlorophyll Fluorescence Parameters ◽  
Psii Photochemistry

Analysis of gas exchange and determination of chlorophyll fluorescence parameters in leaves of three tree species including Aesculus chinensis, A. octandra and A. hybrida were conducted under field conditions and then comparison was carried out. The results showed that the light compensation point (LCP) was significantly different among the three tree species, of which the LCP of A. chinensis with 12.53 μmol·m-2·s-1 that of the lowest was notable lower than that of the other two species (36.11 and 46.41 μmol·m-2·s-1respectively). On the other hand, the light saturation point (LSP) of the three tree species also showed remarked different and the LSP of A. chinensis was 1475 μmol·m-2·s-1 which was dramatic higher than that of the other two species respective to 1366.67 and 1025 μmol·m-2·s-1. Beside, the maximum net photosynthetic rate (MNPR) was different too, MNPR of A. chinensis was 9.47μmol CO 2·m-2·s-1which was higher than the other two species (5.91 and 2.30 μmol CO 2·m-2·s-1 respectively), indicating A. chinensis had a higher photosynthetic capacity and stronger utilization ability for light energy. Moreover, the electron transport rate (ETR) of A. chinensis was higher than A. octandra and A. hybrida, the ETR of the former was 55.800 that were 1.33 and 1.44 times of the later two respectively. Quantum yield of PSII photochemistry (ФPSII) in A. chinensis was higher than A. octandra and A. hybrida, the ФPSII of the former was 0.470 that were 1.21 and 1.15 times of the later two respectively. Furthermore, the photochemical quenching (qP) of A. chinensis was 0.975 much higher than A. octandra and A. hybrida respective to 1.10 and 1.10 times of the later two respectively. These three photochemical parameters with dramatic different among the three different tree species suggested A. chinensis had a high activity of electron transport and conversion efficiency for light energy.

scholarly journals Structure of the Quenched Cyanobacterial OCP-Phycobilisome Complex

2021 ◽  
Author(s):  
Maria Agustina Dominguez-Martin ◽  
Paul V Sauer ◽  
Markus Sutter ◽  
Henning Kirst ◽  
David Bina ◽  
...  
Keyword(s):  
Active Form ◽  
Light Energy ◽  
Structural Basis ◽  
Photochemical Quenching ◽  
Regulatory Domain ◽  
Dynamic Surface ◽  
Photosynthetic Organisms ◽  
Cryo Electron Microscopy ◽  
Orange Carotenoid Protein ◽  
Non Photochemical Quenching

Photoprotection is an essential mechanism in photosynthetic organisms to balance the harvesting of light energy against the risks of photodamage. In cyanobacteria, photoprotective non-photochemical quenching relies on the interaction between a photoreceptor, the Orange Carotenoid Protein (OCP), and the antenna, the phycobilisome (PBS). Here we report the first structure of the OCP-PBS complex at 2.7 Å overall resolution obtained by cryo-electron microscopy. The structure shows that the 6.2 MDa PBS is quenched by four 34 kDa OCP organized as two dimers. The complex also reveals that the structure of the active form of the OCP is drastically different than its resting, non-quenching form, with an ~60 Å displacement of its regulatory domain. These results provide a high-resolution blueprint of the structural basis of the protective quenching of excess excitation energy that enables cyanobacteria to harvest light energy and fix CO2 across environmentally diverse and dynamic surface of our planet.

scholarly journals UV-A/B radiation rapidly activates photoprotective mechanisms in Chlamydomonas reinhardtii

2020 ◽  
Author(s):  
Ryutaro Tokutsu ◽  
Konomi Fujimura-Kamada ◽  
Tomohito Yamasaki ◽  
Keisuke Okajima ◽  
Jun Minagawa
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Red Light ◽  
Action Spectrum ◽  
Biological Significance ◽  
Ultra Violet ◽  
Light Energy ◽  
Photochemical Quenching ◽  
Protein Accumulation ◽  
Unicellular Green Alga ◽  
Non Photochemical Quenching

Conversion of light energy into chemical energy through photosynthesis in the chloroplasts of photosynthetic organisms is essential for photoautotrophic growth. However, the conversion of excess light energy into thermal energy by non-photochemical quenching (NPQ) is important for avoiding the generation of reactive oxygen species and maintaining efficient photosynthesis. In the unicellular green alga Chlamydomonas reinhardtii, NPQ is activated as a photoprotective mechanism through wavelength-specific light signaling pathways mediated by the phototropin (blue light) and UVR8 (ultra-violet light, UV) photoreceptors. NPQ-dependent photoprotection improves cell survival under high-light conditions; however, the biological significance of photoprotection being activated by light with different qualities remains poorly understood. Here, we demonstrate that NPQ-dependent photoprotection is activated more rapidly by UV than by visible light. We found that induction of gene expression and protein accumulation related to photoprotection was significantly faster and greater in magnitude under UV treatment compared to that under blue- or red-light treatment. Furthermore, the action spectrum of UV-dependent induction of photoprotective factors implied that Chlamydomonas sense relatively long-wavelength UV (including UV-A/B), whereas the model dicot plant Arabidopsis thaliana preferentially senses relatively short-wavelength UV (mainly UV-B/C) for induction of photoprotective responses. Therefore, we hypothesize that Chlamydomonas developed a UV response distinct from that of land plants.

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