scholarly journals The Impact of Immunomodulatory Treatment on Kappa Free Light Chains as Biomarker in Neuroinflammation

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 842 ◽  
Author(s):  
Franz Felix Konen ◽  
Ulrich Wurster ◽  
Torsten Witte ◽  
Konstantin Fritz Jendretzky ◽  
Stefan Gingele ◽  
...  
Keyword(s):  
Intravenous Immunoglobulins ◽  
Light Chains ◽  
High Dose ◽  
Free Light Chains ◽  
High Dose Methylprednisolone ◽  
Before And After ◽  
Immune Therapies ◽  
The Stability ◽  
Sample Method ◽  
The Impact

Background: Kappa free light chains (KFLC) are a promising new biomarker to detect neuroinflammation. Still, the impact of pre-analytical effects on KFLC concentrations was not investigated. Methods: KFLC concentrations were measured in serum and cerebrospinal fluid (CSF) of patients with a newly diagnosed multiple sclerosis (MS) or clinically isolated syndrome (CIS) before (n = 42) or after therapy with high-dose methylprednisolone (n = 65). In prospective experiments, KFLC concentrations were analyzed in the same patients in serum before and after treatment with high-dose methylprednisolone (n = 16), plasma exchange (n = 12), immunoadsorption (n = 10), or intravenous immunoglobulins (n = 10). In addition, the influence of storage time, sample method, and contamination of CSF with blood were investigated. Results: Patients diagnosed with MS/CIS and treated with methylprednisolone showed significantly lower KFLC concentrations in serum as untreated patients. Repeated longitudinal investigations revealed that serum KFLC concentrations continuously decreased after each application of methylprednisolone. In contrast, other immune therapies and further pre-analytical conditions did not influence KFLC concentrations. Conclusion: Our results show prominent effects of steroids on KFLC concentrations. In contrast, various other pre-analytical conditions did not influence KFLC concentrations, indicating the stability of this biomarker.

Dosimetric evaluation of methyl red radiochromic film for radiation processing

2020 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Awad AL Zahrany ◽  
Khalid Rabaeh ◽  
Molham Eyadeh ◽  
Ahmed Basfar
Keyword(s):  
Gamma Ray ◽  
Dose Range ◽  
Absorbed Dose ◽  
Radiochromic Film ◽  
High Dose ◽  
Methyl Red ◽  
Radiation Processing ◽  
Content Type ◽  
The Stability ◽  
The Impact

Purpose The purpose of this paper is to present a radiochromic film dosimeter containing polyvinyl alcohol (PVA) matrix and various concentrations of methyl red (MR) dye for high dose measurements. Design/methodology/approach The MR-PVA films were exposed to irradiation up to 60 kGy using 60Co source of gamma ray. The ultraviolet and visible regions (UV/VIS) spectrophotometry were used to examine the optical density of pre-and post-irradiated dosimeters at 424 nm. Findings The dose sensitivity of MR-PVA films increases significantly with increasing MR dye concentrations in the dose range of 5 to 60 kGy. The impact of relative humidity, irradiation temperature, dose rate and the stability of the films has been analyzed. The overall uncertainty of the MR-PVA film dosimeter is 6.12% (Double Standard-deviation, 95% confidence level). Practical implications It was found that the MR-PVA films may be used as high dose dosimeter with an acceptable overall uncertainty in routine industrial radiation processing. Originality/value The color bleaching of irradiated MR-PVA films in terms of specific absorbance curves increases significantly with increasing absorbed dose up to 60 kGy.

scholarly journals Effects Of The Combined Heat And Cryogenic Treatment On The Stability Of Austenite In A High Co-Ni Steel

2015 ◽  
Vol 60 (3) ◽  
pp. 2131-2137 ◽  
Author(s):  
M. Gruber ◽  
S. Ploberger ◽  
G. Ressel ◽  
M. Wiessner ◽  
M. Hausbauer ◽  
...  
Keyword(s):  
Phase Distribution ◽  
Cryogenic Treatment ◽  
Tempering Temperature ◽  
Factors Affecting ◽  
Transmission Electron ◽  
Reverted Austenite ◽  
Before And After ◽  
Different Temperatures ◽  
The Stability ◽  
The Impact

Abstract The stability of austenite is one of the most dominant factors affecting the toughness properties of high Co-Ni steels such as Aermet 100 and AF1410. Thus, the aim of this work was to get a deeper understanding on the impact of combined heat and cryogenic treatment on the stability of retained and reverted austenite. In order to characterize the evolution of the phase fraction of austenite during tempering at different temperatures and times, X-ray diffraction analyses were carried out. The stability of austenite, which was formed during tempering, was analyzed with dilatometric investigations by studying the transformation behavior of the austenite during cooling from tempering temperature down to −100°C. Additionally, transmission electron microscopy investigations were performed to characterize the chemical composition and phase distribution of austenite and martensite before and after tempering.

scholarly journals Stability issues in presence variable distributed generation into radial distribution network

2018 ◽  
Vol 218 ◽  
pp. 01005 ◽  
Author(s):  
Rahman Yuli Asmi ◽  
Agus Siswanto ◽  
Irwan Mahmudi
Keyword(s):  
Distributed Generation ◽  
Voltage Stability ◽  
Reactive Power ◽  
Load Condition ◽  
Stability Index ◽  
Before And After ◽  
Voltage Deviation ◽  
Bus Voltage ◽  
The Stability ◽  
The Impact

Related to environmental issues resulting from the use of traditional energy sources, drive usage of renewable energy is increasing. Changes in the structure of the network will certainly affect the changes in voltage stability. In this paper, discuss the impact of the stability after distributed generation penetration whose its output intermittent relatively. The simulation based PSAT software and tested into IEEE 30 bus system. Observation of voltage deviation and SVSI-index on some load buses in conditions before and after integration of wind generation. Load condition is a very determining factor of bus voltage stability index. This relates to the active power and reactive power needs that must be injected by DG penetration.

scholarly journals The effect of nasal provocation with a single-dose allergen on the physical and cognitive performance of patients with ragweed allergy

2017 ◽  
Vol 104 (4) ◽  
pp. 334-343
Author(s):  
M Tokodi ◽  
E Csábi ◽  
Á Kiricsi ◽  
E Kollár ◽  
AH Molnár ◽  
...  
Keyword(s):  
Working Memory ◽  
Physical Performance ◽  
Cognitive Abilities ◽  
Working Memory Capacity ◽  
Memory Capacity ◽  
Allergen Exposure ◽  
High Dose ◽  
Single Shot ◽  
Before And After ◽  
The Impact

Purpose This study aims to compare the impact of active allergic rhinitis on physical and cognitive abilities of trained allergic athletes to untrained allergic patients. Methods Cognitive, respiratory, and fitness functions were assessed before and after allergen exposure. Participants in both groups were provoked intranasally with ragweed allergen. Results The group of athletes revealed significantly higher average values in peak inspiratory flow and fitness index before and after provocation. In neuropsychological assessments, athletes performed significantly better after allergen provocation in complex working memory capacity. Due to single acute allergen exposure, the size of the nasal cavity and nasal inspiratory peak flow significantly decreased in both groups. The physical performance of both groups did not change after provocation. Executive functions and complex working memory capacity of athletes significantly improved resulting from provocation. Conclusions A single-shot allergen in high dose might cause an increase in mental concentration, which was more pronounced in the group of athletes. This study indicates that acute exposure to allergen cannot affect the physical performance and may result in increased mental focus in patients with allergy notwithstanding the declining respiratory functions.

The impact of a change in prescribing policy on antipsychotic prescribing in a general adult psychiatric hospital

2014 ◽  
Vol 31 (3) ◽  
pp. 167-173 ◽  
Author(s):  
J. Kelly ◽  
F. Kelly ◽  
K. Santlal ◽  
S. O’Ceallaigh
Keyword(s):  
Mental Health ◽  
Psychiatric Hospital ◽  
Antipsychotic Medication ◽  
High Dose ◽  
Evidence Based ◽  
Second Generation Antipsychotics ◽  
Before And After ◽  
Antipsychotic Prescribing ◽  
Clinically Significant ◽  
The Impact

ObjectivesTo examine the impact of a change in local prescribing policy on the adherence to evidence-based prescribing guidelines for antipsychotic medication in a general adult psychiatric hospital.MethodsAll adult in-patients had their clinical record and medication sheet reviewed. Antipsychotic prescribed, dose prescribed and documented indications for prescribing were recorded. This was done before and after the implementation of the change in hospital antipsychotic prescribing policy.ResultsThere were no significant differences in age, sex, Mental Health Act status, psychiatric diagnosis or documented indications for prescribing multiple or high dose antipsychotics between the two groups. There was an increase in the preferential prescribing of multiple second-generation antipsychotics (p=0.01) in the context of a significant reduction in the prescribing of multiple antipsychotics overall (p=0.02). There were no significant reductions in prescribing of mixed generations of antipsychotics (p=0.12), high dose antipsychotics (p=1.00) or as required (PRN) antipsychotics (p=0.74).ConclusionsChanges in local prescribing policy can improve adherence to quality prescribing guidelines and cause clinically significant improvements in patterns of prescribing in a general adult psychiatric hospital.

scholarly journals Defining the impact of individual sample variability on routine immunoassay of serum free light chains (sFLC) in multiple myeloma

2013 ◽  
Vol 171 (2) ◽  
pp. 201-209 ◽  
Author(s):  
S. H. K. Murng ◽  
L. Follows ◽  
P. Whitfield ◽  
J. A. Snowden ◽  
K. Swallow ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Light Chains ◽  
Individual Sample ◽  
Free Light Chains ◽  
Serum Free ◽  
Serum Free Light Chains ◽  
The Impact

scholarly journals Switching from conventional therapy to burosumab injection has the potential to prevent nephrocalcinosis in patients with X-linked hypophosphatemic rickets

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Daisuke Harada ◽  
Kaoru Ueyama ◽  
Kyoko Oriyama ◽  
Yoshihito Ishiura ◽  
Hiroko Kashiwagi ◽  
...  
Keyword(s):  
Conventional Therapy ◽  
Hypophosphatemic Rickets ◽  
High Dose ◽  
Normal Range ◽  
Urine Calcium ◽  
Metabolic Bone ◽  
Renal Phosphate Reabsorption ◽  
Before And After ◽  
Fgf 23 ◽  
The Impact

Abstract Objectives X-linked hypophosphatemic rickets (XLH) is a congenital fibroblast growth factor (FGF)23-related metabolic bone disease that is treated with active vitamin D and phosphate as conventional therapies. Complications of these therapies include nephrocalcinosis (NC) caused by excessive urine calcium and phosphate concentrations. Recently, an anti-FGF23 antibody, burosumab, was developed and reported to be effective in poorly-controlled or severe XLH patients. This study aimed to reveal the impact of switching treatments in relatively well-controlled XLH children with the Rickets Severity Scale less than 2.0. Methods The effects of the two treatments in eight relatively well-controlled XLH children with a mean age of 10.4 ± 1.9 years were compared retrospectively for the same treatment duration (31 ± 11 months) before and after the baseline. Results Actual doses of alfacalcidol and phosphate as conventional therapy were 150.9 ± 43.9 ng/kg and 27.5 ± 6.3 mg/kg per day, respectively. Renal echography revealed spotty NC in 8/8 patients, but no aggravation of NC was detected by switching treatments. Switching treatments increased TmP/GFR (p=0.002) and %TRP (p<0.001), and improved the high urine calcium/creatinine ratio to the normal range (p<0.001) although both treatments controlled disease markers equally. Additionally, low intact parathyroid hormone during conventional therapy was increased within the normal range by switching treatments. Conclusions Our results suggest that a high dose of alfacalcidol was needed to control the disease, but it caused hypercalciuria and NC. We concluded that switching treatments in relatively well-controlled XLH children improved renal phosphate reabsorption and decreased urine calcium extraction, and may have the potential to prevent NC.

scholarly journals Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies

Vaccines ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 267
Author(s):  
Alessandro Torelli ◽  
Elena Gianchecchi ◽  
Martina Monti ◽  
Pietro Piu ◽  
Irene Barneschi ◽  
...  
Keyword(s):  
World Health ◽  
Who Guidelines ◽  
Serum Samples ◽  
Serological Tests ◽  
Freeze Thaw ◽  
Before And After ◽  
The Stability ◽  
Health Organization ◽  
Internal Procedures ◽  
The Impact

Background: Vaccine effectiveness relies on various serological tests, whose aim is the measurement of antibody titer in serum samples collected during clinical trials before and after vaccination. Among the serological assays required by the regulatory authorities to grant influenza vaccine release there are: Hemagglutination inhibition (HAI), microneutralization (MN), and Single Radial Hemolysis (SRH). Although antibodies are regarded to be relatively stable, limited evidences on the effect of multiple freeze–thaw cycles on the stability of antibodies in frozen serum samples are available so far. In view of this, the present paper aimed to evaluate the impact of multiple freeze–thaw cycles on influenza antibody stability, performing HAI, MN and SRH assays. Methods: Ten serum samples were divided into 14 aliquots each, stored at −20 °C and taken through a total of 14 freeze–thaw cycles to assess influenza antibody stability. Each assay measurement was carried out following internal procedures based on World Health Organization (WHO) guidelines. Results: No statistically significant effect of 14 freeze–thaw cycles on antibody stability, measured through three different assays, was observed. Conclusions: Collectively, these data demonstrated that specific influenza antibody present in serum samples are stable up to 14 freeze–thaw cycles.

Effect of Work Discipline and Competency on Employee Performance at Goddes Bakery Bali-Indonesia

2021 ◽  
Vol 8 (12) ◽  
pp. 488-496
Author(s):  
Ida Ayu Sri Meitri ◽  
I Made Setena ◽  
Ni Putu Sri Mariyatni
Keyword(s):  
Regression Analysis ◽  
Raw Materials ◽  
Employee Performance ◽  
Linear Regression Analysis ◽  
Multiple Linear Regression Analysis ◽  
Knowledge And Skills ◽  
Before And After ◽  
Saturated Sample ◽  
Sample Method ◽  
The Impact

The purpose of this study was to examine the impact of the skills and abilities of Goddes Bakery Bali employees and their compliance with all applicable regulations in the company. There are some employees who work in the production department often experience errors with the lack of knowledge and skills of employees, so many breads are rejected which results in a lack of efficiency in raw materials, employees are often absent for no reason even though the company has carried out daily briefings before and after doing work. This will clearly affect the performance of employees and also the performance of the company. Based on this, it is deemed necessary to examine the effect of competence and discipline on employee performance. Data was collected by distributing questionnaires which were used as research instruments. Sampling was carried out using the saturated sample method or the census method, with 34 respondents. Respondents. Data analysis used multiple linear regression analysis. The results show that Work discipline has a positive and significant effect on employee performance at Goodes Bakery Bali. Which means, the higher the employee's work discipline, the employee's performance will increase. Competence has a positive and significant effect on employee performance at Goodes Bakery Bali. Which means, the higher the competence of employees, the performance of employees will increase. Keywords: Performance, Discipline, Competence.

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