scholarly journals SSEA3 and Sialyl Lewis a Glycan Expression Is Controlled by B3GALT5 LTR through Lamin A-NFYA and SIRT1-STAT3 Signaling in Human ES Cells

Cells ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 177
Author(s):  
Bi-He Cai ◽  
Hsueh-Yi Lee ◽  
Chi-Kan Chou ◽  
Po-Han Wu ◽  
Hsiang-Chi Huang ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Es Cells ◽  
Lamin A ◽  
Es Cell ◽  
Stat3 Signaling ◽  
Sialyl Lewis ◽  
Human Es Cells ◽  
Sialyl Lewis A ◽  
Ltr Promoter ◽  
Lewis A

B3GALT5 is involved in the synthesis of embryonic stem (ES) cell marker glycan, stage-specific embryonic antigen-3 (SSEA3). This gene has three native promoters and an integrated retroviral long terminal repeat (LTR) promoter. We found that B3GALT5-LTR is expressed at high levels in human ES cells. B3GALT5-LTR is also involved in the synthesis of the cancer-associated glycan, sialyl Lewis a. Sialyl Lewis a is expressed in ES cells and its expression decreases upon differentiation. Retinoic acid induced differentiation of ES cells, decreased the short form of NFYA (NFYAs), increased phosphorylation of STAT3, and decreased B3GALT5-LTR expression. NFYAs activated, and constitutively-active STAT3 (STAT3C) repressed B3GALT5-LTR promoter. The NFYAs and STAT3C effects were eliminated when their binding sites were deleted. Retinoic acid decreased the binding of NFYA to B3GALT5-LTR promoter and increased phospho-STAT3 binding. Lamin A repressed NFYAs and SSEA3 expression. SSEA3 repression mediated by a SIRT1 inhibitor was reversed by a STAT3 inhibitor. Repression of SSEA3 and sialyl Lewis a synthesis mediated by retinoic acid was partially reversed by lamin A short interfering RNA (siRNA) and a STAT3 inhibitor. In conclusion, B3GALT5-LTR is regulated by lamin A-NFYA and SIRT1-STAT3 signaling that regulates SSEA3 and sialyl Lewis a synthesis in ES cells, and sialyl Lewis a is also a ES cell marker.

Paired tumor marker of soluble E-selectin and its ligand sialyl Lewis A in colorectal cancer

2001 ◽  
Vol 36 (12) ◽  
pp. 823-829 ◽  
Author(s):  
Katsuki Ito ◽  
ChunLin Ye ◽  
Kenji Hibi ◽  
Chikako Mitsuoka ◽  
Reiji Kannagi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Tumor Marker ◽  
Sialyl Lewis ◽  
Sialyl Lewis A ◽  
Soluble E Selectin ◽  
Lewis A

scholarly journals Discovery of sialyl Lewis A and Lewis X modified protein cancer biomarkers using high density antibody arrays

2014 ◽  
Vol 96 ◽  
pp. 291-299 ◽  
Author(s):  
Jung-hyun Rho ◽  
Judson R. Mead ◽  
W. Shea Wright ◽  
Dean E. Brenner ◽  
James W. Stave ◽  
...  
Keyword(s):  
Cancer Biomarkers ◽  
High Density ◽  
Lewis X ◽  
Sialyl Lewis ◽  
Sialyl Lewis A ◽  
Antibody Arrays ◽  
Lewis A

Abstract B122: Human monoclonal antibodies to sialyl‐Lewis‐A with potent CDC and ADCC activity

2009 ◽  
Author(s):  
Ritsuko Sawada ◽  
Shu‐Man Sun ◽  
Feng Hong ◽  
Govind Ragupathi ◽  
Philip O. Livingston ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Adcc Activity ◽  
Human Monoclonal Antibodies ◽  
Sialyl Lewis ◽  
Sialyl Lewis A ◽  
Lewis A

ChemInform Abstract: A Sialyl-Lewis A-Asparagine Building Block for Glycopeptide-Synthesis.

ChemInform ◽  
2000 ◽  
Vol 31 (40) ◽  
pp. no-no
Author(s):  
Karen Peilstoecker ◽  
Horst Kunz
Keyword(s):  
Building Block ◽  
Sialyl Lewis ◽  
Sialyl Lewis A ◽  
Lewis A

Chromatin Profiling of the Globin Loci of Human ES Cells and ES-Derived Erythroid Cells.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 2535-2535
Author(s):  
Xiangdong Fang ◽  
Kai-Hsin Chang ◽  
Daniel Bates ◽  
Morgan Diegel ◽  
Richard Sandstrom ◽  
...  
Keyword(s):  
Cell Lines ◽  
High Throughput ◽  
Es Cells ◽  
Globin Gene ◽  
Dnase I ◽  
Erythroid Cells ◽  
Es Cell ◽  
Hypersensitive Site ◽  
Human Es Cells ◽  
Chromatin Profiling

Abstract Abstract 2535 Poster Board II-512 We used a high throughput approach to determine the chromatin profiles of the human β and α globin loci and their upstream and downstream regions in human undifferentiated ES cells, ES cell-derived erythroid cells, human fetal and adult origin erythroid cells and in primary cells and cell lines of endo-meso and ectodermal origins. All DNase I hypersensitive sites of the b-locus were absent in undifferentiated human ES cells except for HS2 of the b-globin locus control region. The chromatin profiles of the β and α globin loci of ES cell-derived erythroid cells were identical to those of fetal liver erythroid cells except that the hypersensitive site of the embryonic globin gene was more prominent. DNase I hypersensitive site 2 of the b-globin LCR, a potent enhancer, was present in all the cell lines and primary lineages we studied, providing direct evidence that it is ubiquitous. Several new erythroid specific DHSs were detected upstream of 5′HS7 of the β-LCR, raising the possibility that they play a role in the regulation of the β globin locus. The region downstream to 3′HS1 was depleted of DHSs except for the previously identified DHS mapping near the breakpoint of HPFH 1. Since DHSs are absent near the breakpoints of deletional HPFHs and db thalassemias and since enhancers are typically DHS positive, our results argue against the hypothesis of imported enhancers in the pathogenesis of deletional HPFH and db thalassemia mutants. All the previously identified erythroid specific DHSs of the α globin locus were absent in human ES cells. The α globin locus of ES cells, however, displayed three very prominent DHSs, which were located almost symmetrically about 40 Kb apart from each other and they were constitutively formed in all the lineages and cell lines we have studied; the 3′ and 5′ DHSs carried CTCF sites by ChIP-Seq assay raising the possibility that they mark the sites of chromatin insulators. Overall these results demonstrate the power of the new high throughput chromatin profiling approaches and their ability to uncover features of chromatin that may be of regulatory relevance. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Selectin Ligands Sialyl-Lewis a and Sialyl-Lewis x in Gastrointestinal Cancers

Biology ◽  
2017 ◽  
Vol 6 (4) ◽  
pp. 16 ◽  
Author(s):  
Marco Trinchera ◽  
Adele Aronica ◽  
Fabio Dall’Olio
Keyword(s):  
Sialyl Lewis X ◽  
Gastrointestinal Cancers ◽  
Lewis X ◽  
Sialyl Lewis ◽  
Selectin Ligands ◽  
Sialyl Lewis A ◽  
Lewis A

scholarly journals Human embryonic stem cells for brain repair?

2007 ◽  
Vol 363 (1489) ◽  
pp. 87-99 ◽  
Author(s):  
Su-Chun Zhang ◽  
Xue-Jun Li ◽  
M Austin Johnson ◽  
Matthew T Pankratz
Keyword(s):  
Stem Cells ◽  
Motor Neurons ◽  
Es Cells ◽  
Embryonic Stem ◽  
Dopamine Neurons ◽  
Neural Cells ◽  
Es Cell ◽  
Human Es Cells

Cell therapy has been perceived as the main or ultimate goal of human embryonic stem (ES) cell research. Where are we now and how are we going to get there? There has been rapid success in devising in vitro protocols for differentiating human ES cells to neuroepithelial cells. Progress has also been made to guide these neural precursors further to more specialized neural cells such as spinal motor neurons and dopamine-producing neurons. However, some of the in vitro produced neuronal types such as dopamine neurons do not possess all the phenotypes of their in vivo counterparts, which may contribute to the limited success of these cells in repairing injured or diseased brain and spinal cord in animal models. Hence, efficient generation of neural subtypes with correct phenotypes remains a challenge, although major hurdles still lie ahead in applying the human ES cell-derived neural cells clinically. We propose that careful studies on neural differentiation from human ES cells may provide more immediate answers to clinically relevant problems, such as drug discovery, mechanisms of disease and stimulation of endogenous stem cells.

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