scholarly journals Analysis of Allergen-Specific T Cell and IgE Reactivity to Different Preparations of Cow’s Milk-Containing Food Extracts

Cells ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 667 ◽  
Author(s):  
Meng Chen ◽  
Aaron Sutherland ◽  
Giovanni Birrueta ◽  
Susan Laubach ◽  
Stephanie Leonard ◽  
...  
Keyword(s):  
T Cell ◽  
Skin Prick Test ◽  
Food Allergies ◽  
Cow’S Milk ◽  
Cell Reactivity ◽  
Prick Test ◽  
Cow's Milk ◽  
Ige Reactivity ◽  
Significant Difference ◽  
T Cell Reactivity

Background: cow’s milk allergy (CM) is among the most common food allergies in young children and is often outgrown by adulthood. Prior to developing a tolerance to CM, a majority of CM-allergic children may tolerate extensively-heated CM. This study aims to characterize the IgE- and T cell-reactivity to unheated CM and the progressively more heated CM-containing foods. Methods: CM-containing food extracts from muffin, baked cheese, custard and raw, pasteurized CM commercial extract were tested for skin prick test reactivity, IgE binding and T cell reactivity as assessed by IL-5 and IFNγ production. Results: the skin prick test (SPT) reactivity was significantly decreased to muffin extract compared to raw, pasteurized CM. Both IgE- and T-cell reactivity were readily detectable against food extracts from all forms of CM. Western blot analysis of IgE reactivity revealed variability between extracts that was protein-specific. T cell-reactivity was detected against all four extracts with no significant difference in IL-5 or IFNγ production between them. Conclusion: our data indicate that despite reduced clinical reactivity, extracts from heated CM-containing foods retain immunogenicity when tested in vitro, particularly at the T cell level.

scholarly journals Cow's milk–specific T-cell reactivity of children with and without persistent cow's milk allergy: Key role for IL-10

2004 ◽  
Vol 113 (5) ◽  
pp. 932-939 ◽  
Author(s):  
Machteld M. Tiemessen ◽  
Adrie G. Van Ieperen-Van Dijk ◽  
Carla A.F.M. Bruijnzeel-Koomen ◽  
Johan Garssen ◽  
Edward F. Knol ◽  
...  
Keyword(s):  
T Cell ◽  
Cow’S Milk Allergy ◽  
Cow’S Milk ◽  
Milk Allergy ◽  
Cell Reactivity ◽  
Cow's Milk ◽  
Cow's Milk Allergy ◽  
T Cell Reactivity

Is the Skin Prick Test (SPT) for milk useful for diagnosis of Cow's Milk Allergy (CMA) in infants?

2009 ◽  
Vol 123 (2) ◽  
pp. S189-S189
Author(s):  
V.P. Oliveira ◽  
A.B.M. Castro ◽  
A.P.M. Pegas ◽  
A.K. Gushken ◽  
A.C. Pastorino ◽  
...  
Keyword(s):  
Skin Prick Test ◽  
Cow’S Milk Allergy ◽  
Cow’S Milk ◽  
Milk Allergy ◽  
Prick Test ◽  
Cow's Milk ◽  
Cow's Milk Allergy

Chemical modification of peanut conglutin reduces IgE reactivity but not T cell reactivity in peanut-allergic patients

2014 ◽  
Vol 44 (12) ◽  
pp. 1558-1566 ◽  
Author(s):  
E. van Hoffen ◽  
H. P. M. van der Kleij ◽  
C. F. den Hartog Jager ◽  
W. A. van Doorn ◽  
E. F. Knol ◽  
...  
Keyword(s):  
T Cell ◽  
Chemical Modification ◽  
Cell Reactivity ◽  
Ige Reactivity ◽  
T Cell Reactivity

Immune reactivity against a variety of mammalian milks and plant-based milk substitutes

2018 ◽  
Vol 85 (3) ◽  
pp. 358-365 ◽  
Author(s):  
Aristo Vojdani ◽  
Chris Turnpaugh ◽  
Elroy Vojdani
Keyword(s):  
Human Milk ◽  
Cow’S Milk ◽  
Immune Reactivity ◽  
Cow's Milk ◽  
Ige Reactivity ◽  
Sheep Milk ◽  
Significant Difference ◽  
Iga Antibodies ◽  
Milk Substitute ◽  
Milk Substitutes

The research reported here seeks to evaluate the allergenicity and antigenicity of different mammalian and plant-based milks/milk substitutes in healthy subjects. We used ELISA to measure IgE and IgG antibodies against cow, goat, sheep, camel, human milks, and soy, almond, and coconut plant-based milk substitutes, as well as IgA antibodies against all these apart from human milk, in 500 individuals in order to find the percentage of antibody elevation. IgG and IgE positivity showed that human milk was the least antigenic and allergenic, followed by camel milk. Cow's milk showed the highest percentage of elevation or reactivity. Among plant-based milk substitutes, the almond-based substitute was the most allergenic with the highest IgE reactivity, while the coconut milk substitute was lowest. For IgG and IgA immuno-reactivity, soy was first, with coconut again the lowest. We found IgE and IgG immune reactivity against coconut, almond and soymilks in some individuals who were non-reactive to mammalian milk, therefore, we should not assume that consumption of these milks is automatically without risk of allergenic response. We selected 24 samples out of the original 500 for the measurement of IgE antibodies against five different types of cow's milk, from non-organic to organic, A1 and A2. Statistical variance analysis detected no significant difference in IgE, IgG and IgA immune reactivities of the five different cow milks. Our results showed that if an individual is immuno-reactive to cow's milk, organic or not, the probability of reacting to goat and sheep milk is very high. Overall, the results presented here showed that for individuals allergic to cow's milk, the least allergenic alternatives in descending order are human, camel, sheep, and goat milks. Before choosing an alternative for cow's milk, one must go through accurate and quantitative blood testing for determination of IgE, IgG and IgA antibodies against different mammalian and plant-based milks/milk substitutes.

The patch test, skin prick test, and serum milk-specific IgE as diagnostic tools in cow's milk allergy in infants

Allergy ◽  
1999 ◽  
Vol 54 (8) ◽  
pp. 837-842 ◽  
Author(s):  
T Vanto ◽  
A Koivikko ◽  
E Valovirta ◽  
K Juntunen-backman ◽  
T Klemola ◽  
...  
Keyword(s):  
Skin Prick Test ◽  
Patch Test ◽  
Specific Ige ◽  
Cow’S Milk Allergy ◽  
Cow’S Milk ◽  
Diagnostic Tools ◽  
Milk Allergy ◽  
Prick Test ◽  
Cow's Milk ◽  
Cow's Milk Allergy
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