scholarly journals Integration of miRNA and mRNA Co-Expression Reveals Potential Regulatory Roles of miRNAs in Developmental and Immunological Processes in Calf Ileum during Early Growth

Cells ◽  
2018 ◽  
Vol 7 (9) ◽  
pp. 134 ◽  
Author(s):  
Duy Do ◽  
Pier-Luc Dudemaine ◽  
Bridget Fomenky ◽  
Eveline Ibeagha-Awemu
Keyword(s):  
Protein Metabolism ◽  
Intracellular Signaling ◽  
Early Life ◽  
Growth And Development ◽  
Target Genes ◽  
Early Growth ◽  
Mirna Sequencing ◽  
Significant Enrichment ◽  
Mrna Analysis ◽  
Functional Analyses

This study aimed to investigate the potential regulatory roles of miRNAs in calf ileum developmental transition from the pre- to the post-weaning period. For this purpose, ileum tissues were collected from eight calves at the pre-weaning period and another eight calves at the post-weaning period and miRNA expression characterized by miRNA sequencing, followed by functional analyses. A total of 388 miRNAs, including 81 novel miRNAs, were identified. A total of 220 miRNAs were differentially expressed (DE) between the two periods. The potential functions of DE miRNAs in ileum development were supported by significant enrichment of their target genes in gene ontology terms related to metabolic processes and transcription factor activities or pathways related to metabolism (peroxisomes), vitamin digestion and absorption, lipid and protein metabolism, as well as intracellular signaling. Integration of DE miRNAs and DE mRNAs revealed several DE miRNA-mRNA pairs with crucial roles in ileum development (bta-miR-374a—FBXO18, bta-miR-374a—GTPBP3, bta-miR-374a—GNB2) and immune function (bta-miR-15b—IKBKB). This is the first integrated miRNA-mRNA analysis exploring the potential roles of miRNAs in calf ileum growth and development during early life.

scholarly journals Conserved and Divergent Modulation of Calcification in Atherosclerosis and Aortic Valve Disease by Tissue Extracellular Vesicles

2020 ◽  
Author(s):  
Mark C. Blaser ◽  
Fabrizio Buffolo ◽  
Arda Halu ◽  
Florian Schlotter ◽  
Hideyuki Higashi ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Intracellular Signaling ◽  
Target Genes ◽  
Rho Gtpase ◽  
Enzymatic Digestion ◽  
Immunogold Electron Microscopy ◽  
Aortic Valves ◽  
Mirna Sequencing ◽  
Network Approaches ◽  
Gradient Separation

AbstractBackgroundFewer than 50% of patients develop calcification of both atherosclerotic plaques and aortic valves, implying differential pathogenesis. While circulating extracellular vesicles (EVs) act as biomarkers of cardiovascular diseases, tissue-entrapped EVs associate with early mineralization, but their contents, function, and contributions to disease remain unknown.ResultsGlobal proteomics of human carotid artery endarterectomies and calcified aortic valves from a total of 27 donors/patients revealed significant over-representation of proteins with vesicle-associated pathways/ontologies common to both diseases. We exploited enzymatic digestion, serial (ultra)centrifugation and OptiPrep density-gradient separation to isolate EV populations from diseased arteries and valves. Mass spectrometry found 22 EV marker proteins to be highly enriched in the four least-dense OptiPrep fractions while extracellular matrix proteins predominated in denser fractions, as confirmed by CD63 immunogold electron microscopy and nanoparticle tracking analysis. Proteomics and miRNA-sequencing of OptiPrep-enriched tissue EVs quantified 1,104 proteins and 123 miR cargoes linked to 5,182 target genes. Pathway networks of proteins and miR targets common to artery and valve tissue EVs revealed a shared regulation of Rho GTPase and MAPK intracellular signaling cascades. 179 proteins and 5 miRs were significantly altered between artery and valve EVs; multi-omics integration determined that EVs differentially modulated cellular contraction and p53-mediated transcriptional regulation in diseased vascular vs. valvular tissue.ConclusionsOur findings delineate a strategy to isolate, purify, and study protein and RNA cargoes from EVs entrapped in fibrocalcific tissues. Multi-omics and network approaches implicated tissue-resident EVs in human cardiovascular disease.

Studies on establishment of families and their early growth and development for surf clam Mactra chinensis Philipi

2010 ◽  
Vol 34 (4) ◽  
pp. 521-530 ◽  
Author(s):  
Xi-wu YAN ◽  
Qi WANG ◽  
Yue-huan ZHANG ◽  
Zhong-ming HUO ◽  
Yue ZHAO ◽  
...  
Keyword(s):  
Growth And Development ◽  
Early Growth ◽  
Surf Clam ◽  
Mactra Chinensis

scholarly journals Systematic integrated analyses of methylomic and transcriptomic impacts of early combined botanicals on estrogen receptor-negative mammary cancer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Itika Arora ◽  
Yuanyuan Li ◽  
Manvi Sharma ◽  
Michael R. Crowley ◽  
David K. Crossman ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Early Life ◽  
Mammary Cancer ◽  
Epigenetic Modifications ◽  
Green Tea Polyphenols ◽  
Cruciferous Vegetable ◽  
Exciting Potential ◽  
Genomic Scale ◽  
Functional Analyses ◽  
Estrogen Receptor Negative

AbstractDietary botanicals such as the cruciferous vegetable broccoli sprouts (BSp) as well as green tea polyphenols (GTPs) have shown exciting potential in preventing or delaying breast cancer (BC). However, little is known about their impact on epigenomic aberrations that are centrally involved in the initiation and progression of estrogen receptor-negative [ER(−)] BC. We have investigated the efficacy of combined BSp and GTPs diets on mammary tumor inhibition in transgenic Her2/neu mice that were administered the diets from prepubescence until adulthood. Herein, we present an integrated DNA methylome and transcriptome analyses for defining the early-life epigenetic impacts of combined BSp and GTPs on mammary tumors and our results indicate that a combinatorial administration of BSp and GTPs have a stronger impact at both transcriptome and methylome levels in comparison to BSp or GTPs administered alone. We also demonstrated a streamlined approach by performing an extensive preprocessing, quality assessment and downstream analyses on the genomic dataset. Our identification of differentially methylated regions in response to dietary botanicals administered during early-life will allow us to identify key genes and facilitate implementation of the subsequent downstream functional analyses on a genomic scale and various epigenetic modifications that are crucial in preventing ER(−) mammary cancer. Furthermore, our realtime PCR results were also found to be consistent with our genome-wide analysis results. These results could be exploited as a comprehensive resource for understanding understudied genes and their associated epigenetic modifications in response to these dietary botanicals.

The Pleiotropic Intricacies of Hedgehog Signaling: From Craniofacial Patterning to Carcinogenesis

FACE ◽  
2021 ◽  
pp. 273250162110243
Author(s):  
Mikhail Pakvasa ◽  
Andrew B. Tucker ◽  
Timothy Shen ◽  
Tong-Chuan He ◽  
Russell R. Reid
Keyword(s):  
Intracellular Signaling ◽  
Limb Development ◽  
Hedgehog Signaling ◽  
Target Genes ◽  
Craniofacial Development ◽  
Signaling Cascade ◽  
Signaling Mechanisms ◽  
Intracellular Signaling Cascade ◽  
And Function

Hedgehog signaling was discovered more than 40 years ago in experiments demonstrating that it is a fundamental mediator of limb development. Since that time, it has been shown to be important in development, homeostasis, and disease. The hedgehog pathway proceeds through a pathway highly conserved throughout animals beginning with the extracellular diffusion of hedgehog ligands, proceeding through an intracellular signaling cascade, and ending with the activation of specific target genes. A vast amount of research has been done elucidating hedgehog signaling mechanisms and regulation. This research has found a complex system of genetics and signaling that helps determine how organisms develop and function. This review provides an overview of what is known about hedgehog genetics and signaling, followed by an in-depth discussion of the role of hedgehog signaling in craniofacial development and carcinogenesis.

EARLY GROWTH RESPONSE (EGR)-1 AND ITS TARGET GENES CORRELATES WITH LUNG GRAFT FUNCTION IN RATS

2008 ◽  
Vol 86 (Supplement) ◽  
pp. 509
Author(s):  
O Yoshida ◽  
M Yamane ◽  
S Yamamoto ◽  
S Toyooka ◽  
T Ohto ◽  
...  
Keyword(s):  
Growth Response ◽  
Target Genes ◽  
Graft Function ◽  
Early Growth ◽  
Early Growth Response

Biology and development of galls induced by Lopesia sp. (Diptera: Cecidomyiidae) on leaves of Mimosa gemmulata (Leguminosae: Caesalpinioideae)

2018 ◽  
Vol 66 (2) ◽  
pp. 161 ◽  
Author(s):  
Elaine Cotrim Costa ◽  
Renê Gonçalves da Silva Carneiro ◽  
Juliana Santos Silva ◽  
Rosy Mary dos Santos Isaias
Keyword(s):  
Growth And Development ◽  
Glandular Trichomes ◽  
Early Growth ◽  
Vascular Bundles ◽  
Nutritive Tissue ◽  
Development Stages ◽  
First Instar ◽  
Galling Insects ◽  
Late Growth

Analyses of gall biology and development allow determination of morphogenesis events in host-plant organs that are altered by galling insects. Currently, we assume that there is a correlation between Lopesia sp. instars and the alterations in gall tissues on Mimosa gemmulata that generate the gall shape. The development of Lopesia sp. (three larval instars, pupae and adult) correlates positively with gall growth, especially on the anticlinal axis. First-instar larvae are found in galls at the stage of induction, Instar 2 in galls at early growth and development, Instar 3 in galls at late growth and development, pupae in galls at maturation, and the adult emerges from senescent galls. At induction, the larva stimulates cell differentiation in pinnula and pinna-rachis tissues on M. gemmulata. At early growth and development stages, cell division and expansion are increased, and non-glandular trichomes assist gall closing. Homogenous parenchyma and neoformed vascular bundles characterise late growth and development. At maturation, tissues are compartmentalised and cells achieve major expansion through elongation. At senescence, galls open by the falling of trichomes, and mechanical and nutritive cells have thickened walls. The neoformed nutritive tissue nurtures the developing Lopesia sp., whose feeding behaviour influences the direction of cell elongation, predominantly periclinal, determinant for gall bivalve shape.

Moving Targets

2012 ◽  
Author(s):  
Stephen J. Simpson ◽  
David Raubenheimer
Keyword(s):  
Life History ◽  
Natural Selection ◽  
Growth And Development ◽  
Early Growth ◽  
Evolutionary Change ◽  
Life History Strategies ◽  
Moving Targets ◽  
Gene Frequencies ◽  
Epigenetic Effects ◽  
As Species

This chapter studies intake and growth targets. For clarity, earlier chapters have treated intake and growth targets as static points integrated across a particular period in the life of an animal. In reality they are, of course, not static but rather trajectories that move in time. In the short term, the requirements of the animal change as environmental circumstances impose differing demands for nutrients and energy. At a somewhat longer timescale, targets move as the animal passes through the various stages of its life, from early growth and development to maturity, reproduction, and senescence. On an even longer timescale, nutritional traits are subject to natural selection and move as species evolve to exploit new or changing nutritional environments and to adopt differing life-history strategies. Presaging such evolutionary change in gene frequencies within populations are epigenetic effects, whereby the nutritional experiences of parents influence the behavior and metabolism of their offspring without requiring changes in gene frequencies.

Child nutrition

2018 ◽  
pp. 190-194
Author(s):  
Marly Cardoso ◽  
Jonathan Wells
Keyword(s):  
Public Health ◽  
Early Life ◽  
Growth And Development ◽  
Child Nutrition ◽  
Intervention Strategies ◽  
Health Interventions ◽  
Global Burden ◽  
Public Health Interventions ◽  
Good Nutrition ◽  
Healthy Growth

The ‘first 1000 days of life’, covering conception to age two, is recognised as a critical period for the growth and development of young children. Evidence suggests that good nutrition in early life can help adults become taller, stronger, healthier, and more intelligent and, thus, more economically productive, with many benefits for the next generation as well. The concept of a ‘window of opportunity’ is essential for designing intervention strategies. However, how to promote healthy growth in the first 2–3 years, without provoking excess weight gain and adiposity, is a major challenge to public health policymakers. This chapter addresses the global burden of childhood under and over-nutrition, highlighting the main public health interventions for improving child nutrition.

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