scholarly journals Assessing Autophagy in Sciatic Nerves of a Rat Model that Develops Inflammatory Autoimmune Peripheral Neuropathies

Cells ◽  
2017 ◽  
Vol 6 (3) ◽  
pp. 30 ◽  
Author(s):  
Susana Brun ◽  
Nicolas Schall ◽  
Hélène Jeltsch-David ◽  
Jérôme de Sèze ◽  
Sylviane Muller
Keyword(s):  
Rat Model ◽  
Peripheral Neuropathies ◽  
Sciatic Nerves

scholarly journals Bone marrow derived stem cells facilitate axonal regeneration in a rat model of 2, 5-hexanedione-induced neuropathy via miRNA-dependent and independent routes

2021 ◽  
Author(s):  
Enjun Zuo ◽  
Shuhai Hu ◽  
Cong Zhang ◽  
Xiaoxia Shi ◽  
Zhaofei Yang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Stem Cells ◽  
Bone Marrow ◽  
Western Blotting ◽  
Rat Model ◽  
Molecular Mechanisms ◽  
Axon Regeneration ◽  
Autologous Transplantation ◽  
Mtor Inhibitors ◽  
Sciatic Nerves

Abstract Background Peripheral polyneuropathies are clinically difficult situations and can be caused by exposure to occupational hazardous materials, such as N-hexane, which is still being used in developing countries. Autologous transplantation with bone marrow-mesenchymal stem cells (BMSCs) could be a promising therapeutical approach but its effect on toxic chemical-induced neuropathy remains undetermined. The present study sought to understand whether BMSCs could improve axon regeneration in Hexane-caused polyneuropathies and attempted to understand the associated molecular mechanisms. Methods A rat model of 2,5-hexanedine (HD)-induced polyneuropathy was established by intraperitoneally injection of 400 mg/kg/day HD, 5 times/week for 5 weeks. After the development of characteristic phenotypes, rats were given a single bolus tail-vein injection of BMSCs (5×107 cells/kg) and followed-up for 5 weeks. Sciatic nerves of HD/HD + BMSCs treated rats, as a representative tissue for peripheral nerve, were analyzed using electron microscopy, immunohistochemistry, western blotting and RNA sequencing. To further dissect the underlying mechanism, cultured DRG neurons +/- Schwann cells were challenged with HD/BMSC-derived conditional medium (BMSC-CM), in the presence or absence of NGF/Akt/mTOR inhibitors, recombinant NGF, miRNA mimics or miRNA inhibitors and further tested for changes in cellular and molecular levels. Results BMSCs engrafting significantly improved motor function recovery. Accelerated axon regeneration in sciatic nerves was evidenced by transmission electron microscopy, GAP43 western blotting and MBP/SMI312 immunostaining. Increased NGF expression and signaling activation was evidenced in response to BMSC/BMSC-CM treatment. In cultured cells, BMSC-CM’s effect was abolished by anti-NGF antibody or TrkA inhibitor K252a but reinforced by recombinant NGF. The activation of PI3K-Akt-mTOR-CREB signaling was observed and Akt and mTOR inhibitors were shown to attenuate BMSCS-promoted axon regeneration. While NGF was shown to be secreted by BMSCs in culture medium, miRNA array suggested certain miRNAs might also participate to regulate NGF expression. Three let-7 family miRNAs, including let-7a-5p, let-7d-5p and let-7e-5p, and 69 other miRNAs were found to be differentially expressed in HD and HD/BMSC groups. The regulatory effect of let-7e-5p on NGF expression was examined using miRNA mimics and inhibitors. Conclusions Together, our study demonstrated that BMSC transplantation significantly improve axon regeneration in hexane-induced polyneuropathy by activating NGF-PI3K-Akt-mTOR-CREB signaling via two independent mechanisms.

Protective effects of the calcium-channel blocker flunarizine on crush injury of sciatic nerves in a rat model

2010 ◽  
Vol 58 (4) ◽  
pp. 530 ◽  
Author(s):  
Jin-Rong Tang ◽  
Le Wu ◽  
Ping Zhang ◽  
Long-Bin Yu ◽  
Qi Niu ◽  
...  
Keyword(s):  
Calcium Channel ◽  
Calcium Channel Blocker ◽  
Rat Model ◽  
Channel Blocker ◽  
Crush Injury ◽  
Protective Effects ◽  
Sciatic Nerves

1417: PREVENTION OF GLYCOCALYX DEGRADATION ON SCIATIC NERVES BY DANAPAROID SODIUM IN A SEPTIC RAT MODEL

2016 ◽  
Vol 44 (12) ◽  
pp. 430-430
Author(s):  
Hirofumi Hino ◽  
Arisa Miura ◽  
Sachi Shinmi ◽  
Soichiro Inoue
Keyword(s):  
Rat Model ◽  
Danaparoid Sodium ◽  
Sciatic Nerves

Incorporation of Cholesterol into Peripheral Nerve Myelin

1972 ◽  
Vol 30 ◽  
pp. 334-335
Author(s):  
Frank A. Rawlins
Keyword(s):  
Electron Microscope ◽  
Sciatic Nerve ◽  
Electron Microscope Autoradiography ◽  
Myelin Sheaths ◽  
Microscope Autoradiography ◽  
Grain Distribution ◽  
Sciatic Nerves ◽  
Autoradiographic Analysis ◽  
Old Mice ◽  
Short Times

Several speculations exist as to the site of incorporation of preformed molecules into myelin. The possibility that an autoradiographic analysis of cholesterol-1,2-H3 incorporation at very short times after injection might shed some light in the solution of that problem led to the present experiment.Cholesterol-1,2-H3 was injected intraperitoneally into 24 tenday old mice. The animals were then sacrificed at 10,20,30,40,60,90,120 and 180 min after the injection and the sciatic nerves were processed for electron microscope autoradiography. To analyze the grain distribution in the autoradiograms of cross and longitudinal sections from each sciatic nerve myelin sheaths were subdivided into three compartments named: outer 1/3, middle 1/3 and inner 1/3 compartments.It was found that twenty min. after the injection of cholesterol -1.2-H3 (Figs. 1 and 2), 55% of the total number of grains (t.n.g) found in myelin were within the outer 1/3 compartment, 9% were within the middle 1/3 and 36% within the inner 1/3 compartment

1045: Pioglitazone Ameliorates Penile Corpora Veno-Occlusive Dysfunction (CVOD) in a Rat Model of Type 2 Diabetes

2005 ◽  
Vol 173 (4S) ◽  
pp. 283-284
Author(s):  
Istvan Kovanecz ◽  
Monica G. Ferrini ◽  
Hugo H. Davila ◽  
Jacob Rajfer ◽  
Nestor F. Gonzalez-Cadavid
Keyword(s):  
Type 2 Diabetes ◽  
Rat Model

V1341: The Rat Model of Cavernous Nerve Injury: Practical Aspects

2005 ◽  
Vol 173 (4S) ◽  
pp. 364-365
Author(s):  
John F. Donohue ◽  
Michael Mullerad ◽  
Philip S. Li ◽  
Peter T. Scardino ◽  
John P. Mulhall
Keyword(s):  
Nerve Injury ◽  
Rat Model ◽  
Cavernous Nerve ◽  
Cavernous Nerve Injury

1441: Experimental Success of Biodegradable Conduit Graft for Regeneration and Reconnection of Transected Cavernous Nerves in a Rat Model

2004 ◽  
Vol 171 (4S) ◽  
pp. 379-380
Author(s):  
Shinichi Hisasue ◽  
Ryuichi Kato ◽  
Yoshikazu Sato ◽  
Takahiro Suetomi ◽  
Atsushi Takahashi ◽  
...  
Keyword(s):  
Rat Model ◽  
Cavernous Nerves
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