scholarly journals Induction of Cell Death Mechanisms and Apoptosis by Nanosecond Pulsed Electric Fields (nsPEFs)

Cells ◽  
2013 ◽  
Vol 2 (1) ◽  
pp. 136-162 ◽  
Author(s):  
Stephen Beebe ◽  
Nova Sain ◽  
Wei Ren
Keyword(s):  
Cell Death ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Nanosecond Pulsed Electric Fields ◽  
Cell Death Mechanisms

Cell Death Induced by Nanosecond Pulsed Electric Fields and its Dependence on Pulse Duration

2018 ◽  
Vol 101 (3) ◽  
pp. 38-48 ◽  
Author(s):  
RYUYA ADACHI ◽  
SHOTA HATAYAMA ◽  
NOBUAKI OHNISHI ◽  
SUNAO KATSUKI ◽  
TOSHIAKI WADA ◽  
...  
Keyword(s):  
Cell Death ◽  
Pulse Duration ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Nanosecond Pulsed Electric Fields

Cell Death induced by Nanosecond Pulsed Electric Fields and its Dependence on Pulse Duration

2017 ◽  
Vol 137 (6) ◽  
pp. 320-327
Author(s):  
Ryuta Andachi ◽  
Shota Hatayama ◽  
Nobuaki Ohnishi ◽  
Sunao Katsuki ◽  
Toshiaki Wada ◽  
...  
Keyword(s):  
Cell Death ◽  
Pulse Duration ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Nanosecond Pulsed Electric Fields

scholarly journals Towards Solid Tumor Treatment by Nanosecond Pulsed Electric Fields

2009 ◽  
Vol 8 (4) ◽  
pp. 289-306 ◽  
Author(s):  
Axel T. Esser ◽  
Kyle C. Smith ◽  
T. R. Gowrishankar ◽  
James C. Weaver
Keyword(s):  
Cell Death ◽  
Electric Field ◽  
Spatial Scale ◽  
Electric Fields ◽  
Solid Tumor ◽  
Irreversible Electroporation ◽  
Pulsed Electric Fields ◽  
Underlying Mechanism ◽  
Tumor Ablation ◽  
Nanosecond Pulsed Electric Fields

Local and drug-free solid tumor ablation by large nanosecond pulsed electric fields leads to supra-electroporation of all cellular membranes and has been observed to trigger nonthermal cell death by apoptosis. To establish pore-based effects as the underlying mechanism inducing apoptosis, we use a multicellular system model (spatial scale 100 μm) that has irregularly shaped liver cells and a multiscale liver tissue model (spatial scale 200 mm). Pore histograms for the multicellular model demonstrate the presence of only nanometer-sized pores due to nanosecond electric field pulses. The number of pores in the plasma membrane is such that the average tissue conductance during nanosecond electric field pulses is even higher than for longer irreversible electroporation pulses. It is shown, however, that these nanometer-sized pores, although numerous, only significantly change the permeability of the cellular membranes to small ions, but not to larger molecules. Tumor ablation by nanosecond pulsed electric fields causes small to moderate temperature increases. Thus, the underlying mechanism(s) that trigger cell death by apoptosis must be non-thermal electrical interactions, presumably leading to different ionic and molecular transport than for much longer irreversible electroporation pulses.

scholarly journals Nanosecond Pulsed Electric Fields Induce Endoplasmic Reticulum Stress Accompanied by Immunogenic Cell Death in Murine Models of Lymphoma and Colorectal Cancer

Cancers ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 2034 ◽  
Author(s):  
Alessandra Rossi ◽  
Olga N. Pakhomova ◽  
Peter A. Mollica ◽  
Maura Casciola ◽  
Uma Mangalanathan ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Death ◽  
Er Stress ◽  
Cell Lines ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Immunogenic Cell Death ◽  
Cancer Cell Death ◽  
Cell Plasma ◽  
Nanosecond Pulsed Electric Fields

Depending on the initiating stimulus, cancer cell death can be immunogenic or non-immunogenic. Inducers of immunogenic cell death (ICD) rely on endoplasmic reticulum (ER) stress for the trafficking of danger signals such as calreticulin (CRT) and ATP. We found that nanosecond pulsed electric fields (nsPEF), an emerging new modality for tumor ablation, cause the activation of the ER-resident stress sensor PERK in both CT-26 colon carcinoma and EL-4 lymphoma cells. PERK activation correlates with sustained CRT exposure on the cell plasma membrane and apoptosis induction in both nsPEF-treated cell lines. Our results show that, in CT-26 cells, the activity of caspase-3/7 was increased fourteen-fold as compared with four-fold in EL-4 cells. Moreover, while nsPEF treatments induced the release of the ICD hallmark HMGB1 in both cell lines, extracellular ATP was detected only in CT-26. Finally, in vaccination assays, CT-26 cells treated with nsPEF or doxorubicin equally impaired the growth of tumors at challenge sites eliciting a protective anticancer immune response in 78% and 80% of the animals, respectively. As compared to CT-26, both nsPEF- and mitoxantrone-treated EL-4 cells had a less pronounced effect and protected 50% and 20% of the animals, respectively. These results support our conclusion that nsPEF induce ER stress, accompanied by bona fide ICD.

Differential effects of nanosecond pulsed electric fields on cells representing progressive ovarian cancer

2021 ◽  
Vol 142 ◽  
pp. 107942
Author(s):  
Hongmei Liu ◽  
Yajun Zhao ◽  
Chenguo Yao ◽  
Eva M. Schmelz ◽  
Rafael V. Davalos
Keyword(s):  
Ovarian Cancer ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Differential Effects ◽  
Nanosecond Pulsed Electric Fields

scholarly journals Time-Lapse, Live-Cell Imaging of Potassium Efflux in Cell Exposed to Nanosecond Pulsed Electric Fields

2021 ◽  
Vol 120 (3) ◽  
pp. 223a
Author(s):  
Flavia Mazzarda ◽  
Esin B. Sozer ◽  
Julia L. Pittaluga ◽  
Claudia Muratori ◽  
P. Thomas Vernier
Keyword(s):  
Electric Fields ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Pulsed Electric Fields ◽  
Time Lapse ◽  
Live Cell ◽  
Potassium Efflux ◽  
Nanosecond Pulsed Electric Fields
Sign in / Sign up

Export Citation Format

Share Document