scholarly journals Apoptotic Volume Decrease (AVD) Is Independent of Mitochondrial Dysfunction and Initiator Caspase Activation

Cells ◽  
2012 ◽  
Vol 1 (4) ◽  
pp. 1156-1167 ◽  
Author(s):  
Emi Maeno ◽  
Takeshi Tsubata ◽  
Yasunobu Okada
Keyword(s):  
Mitochondrial Dysfunction ◽  
Caspase Activation ◽  
Apoptotic Volume Decrease ◽  
Volume Decrease

scholarly journals Cell Volume Regulation and Apoptotic Volume Decrease in Rat Distal Colon Superficial Enterocytes

2013 ◽  
Vol 32 (6) ◽  
pp. 1551-1565 ◽  
Author(s):  
Stefania Antico ◽  
Maria Giulia Lionetto ◽  
Maria Elena Giordano ◽  
Roberto Caricato ◽  
Trifone Schettino
Keyword(s):  
Cell Volume ◽  
Volume Regulation ◽  
Cell Volume Regulation ◽  
Distal Colon ◽  
Apoptotic Volume Decrease ◽  
Rat Distal Colon ◽  
Volume Decrease

scholarly journals Concentration Dependence of the Antioxidant and Prooxidant Activity of Trolox in HeLa Cells: Involvement in the Induction of Apoptotic Volume Decrease

Antioxidants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1058
Author(s):  
Maria Elena Giordano ◽  
Roberto Caricato ◽  
Maria Giulia Lionetto
Keyword(s):  
Antioxidant Activity ◽  
Vitamin E ◽  
Concentration Dependence ◽  
Hela Cells ◽  
Prooxidant Activity ◽  
Prooxidant Effect ◽  
Apoptotic Process ◽  
Apoptotic Volume Decrease ◽  
Dose Dependent ◽  
Volume Decrease

Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), a hydrophilic analog of vitamin E, is known for its strong antioxidant activity, being a high radical scavenger of peroxyl and alkoxyl radicals. Under particular conditions, Trolox may also exhibit prooxidant properties. The present work aimed at studying the dual antioxidant/prooxidant behavior of Trolox over a wide range of concentrations (from 2.5 to 160 µM) in HeLa cells. In particular, the study addressed the dose-dependent effects of Trolox on the oxidative cell status and vitality of HeLa cells, focusing on the potential role of the vitamin E analog in the induction of one of the first steps of the apoptotic process, Apoptotic Volume Decrease (AVD). In HeLa cells, Trolox showed significant antioxidant activity, expressed as the ability to reduce the endogenous ROS production detected by the ROS-sensitive probe 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate (CM-H2DCFDA), at low concentrations (range: 2.5–15 µM), but exerted a dose-dependent prooxidant effect at higher concentrations after 24 h exposure. The prooxidant effect was paralleled by the reduction in cell viability due to the induction of the apoptotic process. The dual behavior, antioxidant at lower concentrations and prooxidant at higher concentrations, was evident also earlier after 2 h incubation, and it was paralleled by the isotonic shrinkage of the cells, ascribed to AVD. The use of SITS, known Cl− channel blocker, was able to completely inhibit the Trolox-induced isotonic cell shrinkage, demonstrating the involvement of the vitamin E analog in the alteration of cell volume homeostasis and, in turn, in the AVD induction. In conclusion, the study shed light on the concentration dependence of the Trolox antioxidant/prooxidant activity in HeLa cells and revealed its role in the induction of one of the first events of apoptosis, AVD, at high concentrations.

scholarly journals Role of TASK2 in the Control of Apoptotic Volume Decrease in Proximal Kidney Cells

2007 ◽  
Vol 282 (50) ◽  
pp. 36692-36703 ◽  
Author(s):  
Sébastien L'Hoste ◽  
Mallorie Poet ◽  
Christophe Duranton ◽  
Radia Belfodil ◽  
Herv é Barriere ◽  
...  
Keyword(s):  
Kidney Cells ◽  
Apoptotic Volume Decrease ◽  
Volume Decrease

scholarly journals Apoptotic volume decrease as a geometric determinant for cell dismantling into apoptotic bodies

2010 ◽  
Vol 17 (11) ◽  
pp. 1665-1671 ◽  
Author(s):  
R Núñez ◽  
S M Sancho-Martínez ◽  
J M L Novoa ◽  
F J López-Hernández
Keyword(s):  
Apoptotic Bodies ◽  
Apoptotic Volume Decrease ◽  
Volume Decrease

Deregulation of apoptotic volume decrease and ionic movements in multidrug-resistant tumor cells: role of chloride channels

2010 ◽  
Vol 298 (1) ◽  
pp. C14-C25 ◽  
Author(s):  
K. A. Poulsen ◽  
E. C. Andersen ◽  
C. F. Hansen ◽  
T. K. Klausen ◽  
C. Hougaard ◽  
...  
Keyword(s):  
Cell Volume ◽  
Tumor Cells ◽  
Chloride Channels ◽  
Caspase 3 ◽  
Anion Channel ◽  
Multidrug Resistant ◽  
Apoptotic Volume Decrease ◽  
Ehrlich Ascites ◽  
Ionic Movements ◽  
Volume Decrease

Changes in cell volume and ion gradients across the plasma membrane play a pivotal role in the initiation of apoptosis. Here we explore the kinetics of apoptotic volume decrease (AVD) and ion content dynamics in wild-type (WT) and multidrug-resistant (MDR) Ehrlich ascites tumor cells (EATC). In WT EATC, induction of apoptosis with cisplatin (5 μM) leads to three distinctive AVD stages: an early AVD1 (4–12 h), associated with a 30% cell water loss; a transition stage AVDT (∼12 to 32 h), where cell volume is partly recovered; and a secondary AVD2 (past 32 h), where cell volume was further reduced. AVD1 and AVD2 were coupled to net loss of Cl−, K+, Na+, and amino acids (ninhydrin-positive substances), whereas during AVDT, Na+ and Cl− were accumulated. MDR EATC was resistant to cisplatin, showing increased viability and less caspase 3 activation. Compared with WT EATC, MDR EATC underwent a less pronounced AVD1, an augmented AVDT, and a delay in induction of AVD2. Changes in AVD were associated with inhibition of Cl− loss during AVD1, augmented NaCl uptake during AVDT, and a delay of Cl− loss during AVD2. Application of the anion channel inhibitor NS3728 inhibited AVD and completely abolished the differences in AVD, ionic movements, and caspase 3 activation between WT and MDR EATC. Finally, the maximal capacity of volume-regulated anion channel was found to be strongly repressed in MDR EATC. Together, these data suggest that impairment of AVD, primarily via modulation of NaCl movements, contribute to protection against apoptosis in MDR EATC.

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