scholarly journals Proliferation and Differentiation of Intestinal Caco-2 Cells Are Maintained in Culture with Human Platelet Lysate Instead of Fetal Calf Serum

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 3038
Author(s):  
Dalanda Wanes ◽  
Hassan Y. Naim ◽  
Franziska Dengler
Keyword(s):  
Cell Viability ◽  
Fetal Calf Serum ◽  
Human Platelet ◽  
Calf Serum ◽  
Platelet Lysate ◽  
Model Systems ◽  
Structural Protein ◽  
Cell Extracts ◽  
Gene And Protein Expression ◽  
Human Platelet Lysate

Cell lines are widely used as in vitro model systems and substitute for animal experiments. The frequently used Caco-2 cell line is considered to reflect characteristics of differentiated intestinal epithelium. However, the need to culture the cells with fetal calf serum (FCS) induces a high variability, risk of contamination and is ethically disputed. We tested the culture of Caco-2 cells with human platelet lysate (PL) instead of FCS. We compared cell viability and differentiation by measuring ATP levels, gene and protein expression of specific markers in total cell extracts, brush border membrane vesicles (BBM) and lipid rafts (LR). Cell viability was slightly enhanced in cells grown with PL compared to FCS. The cells differentiated to an intestinal phenotype like the cells cultured in FCS, as indicated by the similar gene expression levels of hexose and protein transport proteins and the structural protein VILLIN. BBM showed a comparable distribution of the intestinal hydrolases, indicating a maintained cell membrane polarity. The distribution of the marker protein FLOTILLIN-2 in LR was also similar. We conclude that PL is an exquisite and suitable replacement for FCS in the culture of Caco-2 cells that can eliminate many disadvantages incurred due to the use of FCS.

scholarly journals Human Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Eduardo Fernandez-Rebollo ◽  
Birgit Mentrup ◽  
Regina Ebert ◽  
Julia Franzen ◽  
Giulio Abagnale ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Fetal Calf Serum ◽  
Human Platelet ◽  
Calf Serum ◽  
Platelet Lysate ◽  
Human Platelet Lysate

Human platelet lysate is a feasible candidate to replace fetal calf serum as medium supplement for blood vascular and lymphatic endothelial cells

Cytotherapy ◽  
2014 ◽  
Vol 16 (9) ◽  
pp. 1238-1244 ◽  
Author(s):  
Pablo Hofbauer ◽  
Sabrina Riedl ◽  
Karin Witzeneder ◽  
Florian Hildner ◽  
Susanne Wolbank ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Fetal Calf Serum ◽  
Human Platelet ◽  
Calf Serum ◽  
Platelet Lysate ◽  
Lymphatic Endothelial Cells ◽  
Medium Supplement ◽  
Human Platelet Lysate

scholarly journals Human Platelet Lysate Can Replace Fetal Calf Serum as a Protein Source to Promote Expansion and Osteogenic Differentiation of Human Bone-Marrow-Derived Mesenchymal Stromal Cells

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 918 ◽  
Author(s):  
Maria Karadjian ◽  
Anne-Sophie Senger ◽  
Christopher Essers ◽  
Sebastian Wilkesmann ◽  
Raban Heller ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Fetal Calf Serum ◽  
Culture Media ◽  
Human Platelet ◽  
Calf Serum ◽  
Calcium Content ◽  
Platelet Lysate ◽  
Protein Source ◽  
Human Platelet Lysate

Fetal calf serum (FCS) is frequently used as a growth factor and protein source in bone-marrow-derived mesenchymal stromal cell (BMSC) culture media, although it is a xenogenic product presenting multiple disadvantages including but not limited to ethical concerns. A promising alternative for FCS is human platelet lysate (hPL), which is produced out of human platelet concentrates and happens to be a stable and reliable protein source. In this study, we investigated the influence of hPL in an expansion medium (ESM) and an osteogenic differentiation medium (ODM) on the proliferation and osteogenic differentiation capacity of human BMSC. Therefore, we assessed population doublings during cell expansion, performed alizarin red staining to evaluate the calcium content in the extracellular matrix and determined the activity of alkaline phosphatase (ALP) as osteogenic differentiation correlates. The proliferation rate of BMSC cultured in ESM supplemented with hPL exceeded the proliferation rate of BMSC cultured in the presence of FCS. Furthermore, the calcium content and ALP activity was significantly higher in samples incubated in hPL-supplemented ODM, especially in the early phases of differentiation. Our results show that hPL can replace FCS as a protein supplier in cell culture media and does not negatively affect the osteogenic differentiation capacity of BMSC.

scholarly journals Human platelet lysate delivered via an ocular wound chamber for the treatment of corneal epithelial injuries

2021 ◽  
Vol 206 ◽  
pp. 108493
Author(s):  
Gina L. Griffith ◽  
Andrew W. Holt ◽  
Elof Eriksson ◽  
Anthony J. Johnson ◽  
Jennifer S. McDaniel
Keyword(s):  
Human Platelet ◽  
Platelet Lysate ◽  
Corneal Epithelial ◽  
Human Platelet Lysate

scholarly journals Human platelet lysate improves human cord blood derived ECFC survival and vasculogenesis in three dimensional (3D) collagen matrices

2015 ◽  
Vol 101 ◽  
pp. 72-81 ◽  
Author(s):  
Hyojin Kim ◽  
Nutan Prasain ◽  
Sasidhar Vemula ◽  
Michael J. Ferkowicz ◽  
Momoko Yoshimoto ◽  
...  
Keyword(s):  
Cord Blood ◽  
Human Platelet ◽  
Three Dimensional ◽  
Platelet Lysate ◽  
Human Cord Blood ◽  
Collagen Matrices ◽  
Human Platelet Lysate ◽  
3D Collagen

Human platelet lysate: Replacing fetal bovine serum as a gold standard for human cell propagation?

Biomaterials ◽  
2016 ◽  
Vol 76 ◽  
pp. 371-387 ◽  
Author(s):  
Thierry Burnouf ◽  
Dirk Strunk ◽  
Mickey B.C. Koh ◽  
Katharina Schallmoser
Keyword(s):  
Human Cell ◽  
Gold Standard ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
Human Platelet Lysate ◽  
Fetal Bovine ◽  
Cell Propagation
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