scholarly journals Death-Associated Protein Kinase 1 Inhibits Progression of Thyroid Cancer by Regulating Stem Cell Markers

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2994
Author(s):  
Mi-Hyeon You ◽  
Woo Kyung Lee ◽  
Meihua Jin ◽  
Dong Eun Song ◽  
Sheue-yann Cheng ◽  
...  
Keyword(s):  
Stem Cell ◽  
Thyroid Cancer ◽  
Protein Kinase ◽  
Cell Lines ◽  
Cancer Metastasis ◽  
Thyroid Tissue ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Public Data ◽  
Death Associated Protein Kinase

The activation of metastatic reprogramming is vital for cancer metastasis, but little is known about its mechanism. This study investigated the potential role of death-associated protein kinase 1 (DAPK1) in thyroid cancer progression. We generated knockdown (KD) DAPK1 using siRNA or shRNA in 8505C and KTC-1 cell lines, which we transiently or stably overexpressed in MDA-T32 and BCPAP cell lines. DAPK1 KD in 8505C and KTC-1 cells significantly increased cell proliferation and colony formation compared with controls. We observed significant inhibition of cancer cell invasion in cells overexpressing DAPK1, but the opposite effect in KD cells. Tumorsphere formation significantly increased after inhibition of DAPK1 expression in 8505C cells and was significantly suppressed in DAPK1-overexpressing MDA-T32 and BCPAP cells. DAPK1 overexpression inhibited mRNA and protein levels of stem markers (OCT4, Sox2, KLF4, and Nanog). Furthermore, the expression of these markers increased after KD of DAPK1 in 8505C cells. Mechanistic studies suggest that DAPK1 may modulate the expression of stem cell markers through the inhibition of β-catenin pathways. These findings were consistent with the public data and our thyroid tissue analysis, which showed higher DAPK1 expression was associated with advanced-stage papillary thyroid cancer with a higher stemness index and lower disease-free survival.

scholarly journals Focal Adhesion Kinase Inhibition Contributes to Tumor Cell Survival and Motility in Neuroblastoma Patient-Derived Xenografts

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Laura L. Stafman ◽  
Adele P. Williams ◽  
Raoud Marayati ◽  
Jamie M. Aye ◽  
Hooper R. Markert ◽  
...  
Keyword(s):  
Stem Cell ◽  
Tumor Cell ◽  
Cell Survival ◽  
Cell Lines ◽  
Human Condition ◽  
Migration And Invasion ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Cycle Arrest

Abstract Patient-derived xenografts (PDXs) provide an opportunity to evaluate the effects of therapies in an environment that more closely resembles the human condition than that seen with long-term passage cell lines. In the current studies, we investigated the effects of FAK inhibition on two neuroblastoma PDXs in vitro. Cells were treated with two small molecule inhibitors of FAK, PF-573,228 (PF) and 1,2,4,5-benzentetraamine tetrahydrochloride (Y15). Following FAK inhibition, cell survival and proliferation decreased significantly and cell cycle arrest was seen in both cell lines. Migration and invasion assays were used to determine the effect of FAK inhibition on cell motility, which decreased significantly in both cell lines in the presence of either inhibitor. Finally, tumor cell stemness following FAK inhibition was evaluated with extreme limiting dilution assays as well as with immunoblotting and quantitative real-time PCR for the expression of stem cell markers. FAK inhibition decreased formation of tumorspheres and resulted in a corresponding decrease in established stem cell markers. FAK inhibition decreased many characteristics of the malignant phenotype, including cancer stem cell like features in neuroblastoma PDXs, making FAK a candidate for further investigation as a potential target for neuroblastoma therapy.

Relationships between multidrug resistance (MDR) and stem cell markers in human chronic myeloid leukemia cell lines

2010 ◽  
Vol 34 (6) ◽  
pp. 757-762 ◽  
Author(s):  
Daiane S. Marques ◽  
Juliana Z. Sandrini ◽  
Robert T. Boyle ◽  
Luis F. Marins ◽  
Gilma S. Trindade
Keyword(s):  
Stem Cell ◽  
Multidrug Resistance ◽  
Chronic Myeloid Leukemia ◽  
Cell Lines ◽  
Myeloid Leukemia ◽  
Leukemia Cell ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Leukemia Cell Lines ◽  
Myeloid Leukemia Cell

scholarly journals Cancer-initiating cells derived from established cervical cell lines exhibit stem-cell markers and increased radioresistance

BMC Cancer ◽  
2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Jacqueline López ◽  
Adela Poitevin ◽  
Veverly Mendoza-Martínez ◽  
Carlos Pérez-Plasencia ◽  
Alejandro García-Carrancá
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Cervical Cell ◽  
Increased Radioresistance

scholarly journals Analysis of Circulating Tumour Cells in Early-Stage Uveal Melanoma: Evaluation of Tumour Marker Expression to Increase Capture

Cancers ◽  
2021 ◽  
Vol 13 (23) ◽  
pp. 5990
Author(s):  
Aaron B. Beasley ◽  
Timothy W. Isaacs ◽  
Tersia Vermeulen ◽  
James Freeman ◽  
Jean-Louis DeSousa ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Uveal Melanoma ◽  
Early Stage ◽  
Tumour Cells ◽  
Stem Cell Markers ◽  
Circulating Tumour Cells ◽  
Marker Panel ◽  
Cell Markers ◽  
Molecular Features

(1) Background: The stratification of uveal melanoma (UM) patients into prognostic groups is critical for patient management and for directing patients towards clinical trials. Current classification is based on clinicopathological and molecular features of the tumour. Analysis of circulating tumour cells (CTCs) has been proposed as a tool to avoid invasive biopsy of the primary tumour. However, the clinical utility of such liquid biopsy depends on the detection rate of CTCs. (2) Methods: The expression of melanoma, melanocyte, and stem cell markers was tested in a primary tissue microarray (TMA) and UM cell lines. Markers found to be highly expressed in primary UM were used to either immunomagnetically isolate or immunostain UM CTCs prior to treatment of the primary lesion. (3) Results: TMA and cell lines had heterogeneous expression of common melanoma, melanocyte, and stem cell markers. A multi-marker panel of immunomagnetic beads enabled isolation of CTCs in 37/43 (86%) patients with UM. Detection of three or more CTCs using the multi-marker panel, but not MCSP alone, was a significant predictor of shorter progression free (p = 0.040) and overall (p = 0.022) survival. (4) Conclusions: The multi-marker immunomagnetic isolation protocol enabled the detection of CTCs in most primary UM patients. Overall, our results suggest that a multi-marker approach could be a powerful tool for CTC separation for non-invasive prognostication of UM.

scholarly journals Osteopontin Overexpression Induced Tumor Progression and Chemoresistance to Oxaliplatin through Induction of Stem-Like Properties in Human Colorectal Cancer

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Lui Ng ◽  
Timothy Wan ◽  
Ariel Chow ◽  
Deepak Iyer ◽  
Johnny Man ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Stem Cell ◽  
Poor Prognosis ◽  
Cancer Metastasis ◽  
Tumor Stage ◽  
Clinicopathological Parameters ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Response To Chemotherapy

Colorectal cancer (CRC) is one of the most common and fatal malignancies worldwide. The poor prognosis of colorectal cancer patients is due to development of chemoresistance and cancer metastasis. Recently osteopontin (OPN) has been associated with stem-like properties in colorectal cancer. This study further examined the clinicopathological significance of OPN in CRC and its effect on chemoresistance and transcription of stem cell markers. We examined the transcription level of OPN in 84 CRC patients and correlated the expression with their clinicopathological parameters. The associations of OPN overexpression with transcription of stem cell markers and response to chemotherapy in DLD1-OPN overexpressing clones and CRC patients were also investigated. Our results showed that OPN was significantly overexpressed in CRC, and its overexpression correlated with tumor stage and poor prognosis. Overexpression of CRC induced OCT4 and SOX2 expressionin vitroand correlated with SOX2 overexpression in CRC patients. In addition, DLD1-OPN overexpressing cells showed enhanced ability to survive upon oxaliplatin treatment, and OPN expression was higher in CRC patients who were resistant to oxaliplatin-involved chemotherapy treatment. Thus, CRC cells overexpressing OPN demonstrated stem-like properties and OPN inhibition is a potential therapeutic approach to combat CRC progression and chemoresistance.

Pancreatic Carcinoma Cell Lines Reflect Frequency and Variability of Cancer Stem Cell Markers in Clinical Tissue

2012 ◽  
Vol 49 (2) ◽  
pp. 88-98 ◽  
Author(s):  
S. Bünger ◽  
M. Barow ◽  
C. Thorns ◽  
S. Freitag-Wolf ◽  
S. Danner ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cancer Stem Cell ◽  
Pancreatic Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Cancer Stem Cell Markers ◽  
Carcinoma Cell Lines
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