scholarly journals Steatosis Alters the Activity of Hepatocyte Membrane Transporters in Obese Rats

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2733
Author(s):  
Catherine M Pastor ◽  
Valérie Vilgrain
Keyword(s):  
Contrast Agent ◽  
Experimental Model ◽  
Liver Diseases ◽  
Gadobenate Dimeglumine ◽  
Membrane Transporters ◽  
Liver Imaging ◽  
Fat Accumulation ◽  
Obese Rats ◽  
Rat Livers ◽  
Gamma Counter

Fat accumulation (steatosis) in ballooned hepatocytes alters the expression of membrane transporters in Zucker fatty (fa/fa) rats. The aim of the study was to quantify the functions of these transporters and their impact on hepatocyte concentrations using a clinical hepatobiliary contrast agent (Gadobenate dimeglumine, BOPTA) for liver imaging. In isolated and perfused rat livers, we quantified BOPTA accumulation and decay profiles in fa/+ (normal) and fa/fa hepatocytes by placing a gamma counter over livers. Profiles of BOPTA accumulation and decay in hepatocytes were analysed with nonlinear regressions to characterise BOPTA influx and efflux across hepatocyte transporters. At the end of the accumulation period, BOPTA hepatocyte concentrations and influx clearances were not significantly different in fa/+ and fa/fa livers. In contrast, bile clearance was significantly lower in fatty hepatocytes while efflux clearance back to sinusoids compensated the low efflux into canaliculi. The time when BOPTA cellular efflux impacts the accumulation profile of hepatocyte concentrations was slightly delayed (2 min) by steatosis, anticipating a delayed emptying of hepatocytes. The experimental model is useful for quantifying the functions of hepatocyte transporters in liver diseases.

scholarly journals Isolated Perfused Rat Livers to Quantify the Pharmacokinetics and Concentrations of Gd-BOPTA

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Catherine M. Pastor
Keyword(s):  
Contrast Agent ◽  
Contrast Agents ◽  
Image Interpretation ◽  
Gadobenate Dimeglumine ◽  
Pharmacokinetic Parameters ◽  
Liver Imaging ◽  
Transfer Rates ◽  
Set Up ◽  
Rat Livers

With recent advances in liver imaging, the estimation of liver concentrations is now possible following the injection of hepatobiliary contrast agents and radiotracers. However, how these images are generated remains partially unknown. Most experiments that would be helpful to increase this understanding cannot be performed in vivo. For these reasons, we investigated the liver distribution of the magnetic resonance (MR) contrast agent gadobenate dimeglumine (Gd-BOPTA, MultiHance®, Bracco Imaging) in isolated perfused rat livers (IPRLs). In IPRL, we developed a new set up that quantifies simultaneously the Gd-BOPTA compartment concentrations and the transfer rates between these compartments. Concentrations were measured either by MR signal intensity or by count rates when the contrast agent was labelled by [153Gd]. With this experimental model, we show how the Gd-BOPTA hepatocyte concentrations are modified by temperature and liver flow rates. We define new pharmacokinetic parameters to quantify the canalicular transport of Gd-BOPTA. Finally, we present how transfer rates generate Gd-BOPTA concentrations in rat liver compartments. These findings better explain how liver imaging with hepatobiliary radiotracers and contrast agents is generated and improve the image interpretation by clinicians.

Evaluation of gadoxetate disodium as a contrast agent for mouse liver imaging: comparison with gadobenate dimeglumine

2009 ◽  
Vol 27 (1) ◽  
pp. 101-107 ◽  
Author(s):  
Shigeru Kiryu ◽  
Yusuke Inoue ◽  
Makoto Watanabe ◽  
Kiyoko Izawa ◽  
Morio Shimada ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Mouse Liver ◽  
Gadobenate Dimeglumine ◽  
Liver Imaging ◽  
Gadoxetate Disodium

A combination of borage seed oil and quercetin reduces fat accumulation and improves insulin sensitivity in obese rats

2020 ◽  
Vol 11 (5) ◽  
pp. 4512-4524 ◽  
Author(s):  
Paula Aranaz ◽  
María Zabala ◽  
Ana Romo-Hualde ◽  
David Navarro-Herrera ◽  
Miguel López-Yoldi ◽  
...  
Keyword(s):  
Insulin Sensitivity ◽  
Glucose Tolerance ◽  
Seed Oil ◽  
Liver Steatosis ◽  
Key Factors ◽  
Fat Accumulation ◽  
Obese Rats

Borage seed oil (BSO) combined with quercetin improves glucose tolerance, insulin sensitivity, liver steatosis and adiposity in pre-obese rats. The combination also inhibits adipogenesis in vitro by affecting some adipogenesis-key factors.

Evaluation of the hepatocyte-specific contrast agent gadobenate dimeglumine for MR imaging of acute hepatitis in a rat model

1997 ◽  
Vol 7 (1) ◽  
pp. 147-152 ◽  
Author(s):  
Pasquina Marzola ◽  
Fabio Maggioni ◽  
Eleonora Vicinanza ◽  
Massimo Daprà ◽  
Friedrich M. Cavagna
Keyword(s):  
Mr Imaging ◽  
Contrast Agent ◽  
Acute Hepatitis ◽  
Rat Model ◽  
Gadobenate Dimeglumine

scholarly journals MRI monitoring of Avastin™ antiangiogenesis therapy using B22956/1, a new blood pool contrast agent, in an experimental model of human cancer

2004 ◽  
Vol 20 (5) ◽  
pp. 865-873 ◽  
Author(s):  
Anda Preda ◽  
Viktor Novikov ◽  
Martina Möglich ◽  
Karl Turetschek ◽  
David M. Shames ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Experimental Model ◽  
Human Cancer ◽  
Blood Pool ◽  
Blood Pool Contrast Agent ◽  
Antiangiogenesis Therapy
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