scholarly journals Co-Expression of CD34, CD90, OV-6 and Cell-Surface Vimentin Defines Cancer Stem Cells of Hepatoblastoma, Which Are Affected by Hsp90 Inhibitor 17-AAG

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2598
Author(s):  
Mieun Lee-Theilen ◽  
Julia R. Hadhoud ◽  
Giulietta Volante ◽  
Delaine D. Fadini ◽  
Julia Eichhorn ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cell Surface ◽  
Hsp90 Inhibitor ◽  
Chemotherapeutic Drug ◽  
Therapeutic Approaches ◽  
Pediatric Solid Tumors ◽  
Tumor Research ◽  
And Migration ◽  
Emt Markers

Cancer stem cells (CSCs) are nowadays one of the major focuses in tumor research since this subpopulation was revealed to be a great obstacle for successful treatment. The identification of CSCs in pediatric solid tumors harbors major challenges because of the immature character of these tumors. Here, we present CD34, CD90, OV-6 and cell-surface vimentin (csVimentin) as reliable markers to identify CSCs in hepatoblastoma cell lines. We were able to identify CSC characteristics for the subset of CD34+CD90+OV-6+csVimentin+-co-expressing cells, such as pluripotency, self-renewal, increased expression of EMT markers and migration. Treatment with Cisplatin as the standard chemotherapeutic drug in hepatoblastoma therapy further revealed the chemo-resistance of this subset, which is a main characteristic of CSCs. When we treated the cells with the Hsp90 inhibitor 17-AAG, we observed a significant reduction in the CSC subset. With our study, we identified CSCs of hepatoblastoma using CD34, CD90, OV-6 and csVimentin. This set of markers could be helpful to estimate the success of novel therapeutic approaches, as resistant CSCs are responsible for tumor relapses.

scholarly journals Therapeutic Approaches to Target Cancer Stem Cells

Cancers ◽  
2011 ◽  
Vol 3 (3) ◽  
pp. 3331-3352 ◽  
Author(s):  
Arlhee Diaz ◽  
Kalet Leon
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Therapeutic Approaches ◽  
Target Cancer

scholarly journals Chronic Oxidative Stress Promotes Molecular Changes Associated with Epithelial Mesenchymal Transition, NRF2, and Breast Cancer Stem Cell Phenotype

Antioxidants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 633 ◽  
Author(s):  
Ana Čipak Gašparović ◽  
Lidija Milković ◽  
Nadia Dandachi ◽  
Stefanie Stanzer ◽  
Iskra Pezdirc ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Therapy Resistance ◽  
Mesenchymal Transition ◽  
Chronic Oxidative Stress ◽  
Emt Markers

Oxidative stress plays a role in carcinogenesis, but it also contributes to the modulation of tumor cells and microenvironment caused by chemotherapeutics. One of the consequences of oxidative stress is lipid peroxidation, which can, through reactive aldehydes such as 4-hydroxy-2-nonenal (HNE), affect cell signaling pathways. On the other hand, cancer stem cells (CSC) are now recognized as a major factor of malignancy by causing metastasis, relapse, and therapy resistance. Here, we evaluated whether oxidative stress and HNE modulation of the microenvironment can influence CSC growth, modifications of the epithelial to mesenchymal transition (EMT) markers, the antioxidant system, and the frequency of breast cancer stem cells (BCSC). Our results showed that oxidative changes in the microenvironment of BCSC and particularly chronic oxidative stress caused changes in the proliferation and growth of breast cancer cells. In addition, changes associated with EMT, increase in glutathione (GSH) and Nuclear factor erythroid 2-related factor 2 (NRF2) were observed in breast cancer cells grown on HNE pretreated collagen and under chronic oxidative stress. Our results suggest that chronic oxidative stress can be a bidirectional modulator of BCSC fate. Low levels of HNE can increase differentiation markers in BCSC, while higher levels increased GSH and NRF2 as well as certain EMT markers, thereby increasing therapy resistance.

scholarly journals Therapeutic approaches targeting cancer stem cells

2018 ◽  
Vol 14 (7) ◽  
pp. 1469 ◽  
Author(s):  
Chuanwu Zhu ◽  
Yunzhi Pan ◽  
Sai Ma ◽  
Kaiyue Cao ◽  
Sufang Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Therapeutic Approaches

Targeting Cancer Stem Cells Pathways for the Effective Treatment of Cancer

2020 ◽  
Vol 21 (3) ◽  
pp. 258-278 ◽  
Author(s):  
Ashish Ranjan Dwivedi ◽  
Amandeep Thakur ◽  
Vijay Kumar ◽  
Ira Skvortsova ◽  
Vinod Kumar
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Effective Treatment ◽  
Single Agent ◽  
Clinical Results ◽  
Review Article ◽  
Therapeutic Approaches ◽  
Design Synthesis ◽  
Complete Eradication ◽  
Current Review

Resistance to chemotherapy and relapse are major hurdles for the effective treatment of cancer. Major reason for this is a small sub population of cancer stem cells (CSCs) and its microenvironment. CSCs are critical driving force for several types of cancer, such as gastric, colon, breast and many more. Hence, for the complete eradication of cancer, it is necessary to develop therapeutic approaches that can specifically target CSCs. Chemical agents that target different proteins involved in CSC signaling pathways, either as single agent or simultaneously targeting two or more proteins have generated promising pre-clinical and clinical results. In the current review article, we have discussed various targets and cellular pathways that can be explored for the effective and complete eradication of CSCs. Some latest developments in the field of design, synthesis and screening of ligands to target cancer stem cells have been summarized in the current review article.

Cancer Stem Cells Equipped with Powerful Hedgehog Signaling and Better Epigenetic Memory: Avenues to Look for Cancer Therapeutics

2019 ◽  
Vol 19 (11) ◽  
pp. 877-884 ◽  
Author(s):  
Ishita Tandon ◽  
Asawari Waghmode ◽  
Nilesh Kumar Sharma
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Sonic Hedgehog ◽  
Hedgehog Signaling ◽  
Cancer Therapeutics ◽  
Complex Nature ◽  
Epigenetic Memory ◽  
Therapeutic Approaches ◽  
Shh Signaling ◽  
Memory Power

Complex nature of the tumor is depicted at the cellular landscape by showing heterogeneity in the presence of cancer cells, cancer-associated stromal cells, mesenchymal stem cells and cancer stem cells (CSCs). One of the plausible views in cancer formation is suggested as the theory of cancer CSCs that is known as a source of initiation of tumorigenesis. In essence, these powerful CSCs are equipped with high Sonic Hedgehog (SHH) signaling and epigenetic memory power that support various tumor hallmarks. Truly, nature justifies its intent by limiting these stem cells with a potential to turn into CSCs and in turn suppressing the high risk of humans and other organisms. In short, this mini-review addresses the contribution of SHH signaling to allow reprogramming of epigenetic memory within CSCs that support tumor hallmarks. Besides, this paper explores therapeutic approaches to mitigate SHH signaling that may lead to a blockade of the pro-tumor potential of CSCs.

CD133 and CD44 Cell surface markers do not identify cancer stem cells in primary human gastric tumors

2012 ◽  
Vol 227 (6) ◽  
pp. 2686-2693 ◽  
Author(s):  
Alba Rocco ◽  
Eleonora Liguori ◽  
Giuseppe Pirozzi ◽  
Virginia Tirino ◽  
Debora Compare ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cell Surface ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Gastric Tumors
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